Inhibition of DNA synthesis in human gliomas by roscovitine
THE early effect of 1–100 μM roscovitine, a purine analogue and cyclin-dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [H]methylthymidine plus vehicle alone or the proper concent...
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| Veröffentlicht in: | Neuroreport 1999-08, Vol.10 (12), p.2563-2567 |
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| container_title | Neuroreport |
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| creator | Yakisich, J Sebastian Boethius, Jörgen Lindblom, Ingrid Ohlsson Wallstedt, Lars Vargas, Victor Idoyaga Sidén, Åke Cruz, Mabel H |
| description | THE early effect of 1–100 μM roscovitine, a purine analogue and cyclin-dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [H]methylthymidine plus vehicle alone or the proper concentration of roscovitine for 30–90 min. The DNA synthesis rate was assessed by measurement of [H]methylthymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 μM roscovitine inhibited DNA synthesis by 71–97% (average 89 ± 8%, p |
| doi_str_mv | 10.1097/00001756-199908200-00023 |
| format | Article |
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The tissue was incubated immediately after resection with DMEM containing [H]methylthymidine plus vehicle alone or the proper concentration of roscovitine for 30–90 min. The DNA synthesis rate was assessed by measurement of [H]methylthymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 μM roscovitine inhibited DNA synthesis by 71–97% (average 89 ± 8%, p <0.0001). This inhibitory effect of roscovitine appeared within 30 min of incubation and was concentration dependent.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/00001756-199908200-00023</identifier><identifier>PMID: 10574370</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Adult ; Antineoplastic agents ; Antineoplastic Agents - therapeutic use ; Biological and medical sciences ; Chemotherapy ; DNA, Neoplasm - biosynthesis ; Female ; Glioblastoma - drug therapy ; Glioblastoma - metabolism ; Humans ; Male ; Medical sciences ; Middle Aged ; Neoplasm Proteins - analysis ; Pharmacology. 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The tissue was incubated immediately after resection with DMEM containing [H]methylthymidine plus vehicle alone or the proper concentration of roscovitine for 30–90 min. The DNA synthesis rate was assessed by measurement of [H]methylthymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 μM roscovitine inhibited DNA synthesis by 71–97% (average 89 ± 8%, p <0.0001). This inhibitory effect of roscovitine appeared within 30 min of incubation and was concentration dependent.</description><subject>Adult</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Chemotherapy</subject><subject>DNA, Neoplasm - biosynthesis</subject><subject>Female</subject><subject>Glioblastoma - drug therapy</subject><subject>Glioblastoma - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - analysis</subject><subject>Pharmacology. 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Drug treatments</topic><topic>Purines - therapeutic use</topic><topic>Roscovitine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yakisich, J Sebastian</creatorcontrib><creatorcontrib>Boethius, Jörgen</creatorcontrib><creatorcontrib>Lindblom, Ingrid Ohlsson</creatorcontrib><creatorcontrib>Wallstedt, Lars</creatorcontrib><creatorcontrib>Vargas, Victor Idoyaga</creatorcontrib><creatorcontrib>Sidén, Åke</creatorcontrib><creatorcontrib>Cruz, Mabel H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yakisich, J Sebastian</au><au>Boethius, Jörgen</au><au>Lindblom, Ingrid Ohlsson</au><au>Wallstedt, Lars</au><au>Vargas, Victor Idoyaga</au><au>Sidén, Åke</au><au>Cruz, Mabel H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of DNA synthesis in human gliomas by roscovitine</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>1999-08-20</date><risdate>1999</risdate><volume>10</volume><issue>12</issue><spage>2563</spage><epage>2567</epage><pages>2563-2567</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>THE early effect of 1–100 μM roscovitine, a purine analogue and cyclin-dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [H]methylthymidine plus vehicle alone or the proper concentration of roscovitine for 30–90 min. The DNA synthesis rate was assessed by measurement of [H]methylthymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 μM roscovitine inhibited DNA synthesis by 71–97% (average 89 ± 8%, p <0.0001). This inhibitory effect of roscovitine appeared within 30 min of incubation and was concentration dependent.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>10574370</pmid><doi>10.1097/00001756-199908200-00023</doi><tpages>5</tpages></addata></record> |
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| ispartof | Neuroreport, 1999-08, Vol.10 (12), p.2563-2567 |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adult Antineoplastic agents Antineoplastic Agents - therapeutic use Biological and medical sciences Chemotherapy DNA, Neoplasm - biosynthesis Female Glioblastoma - drug therapy Glioblastoma - metabolism Humans Male Medical sciences Middle Aged Neoplasm Proteins - analysis Pharmacology. Drug treatments Purines - therapeutic use Roscovitine |
| title | Inhibition of DNA synthesis in human gliomas by roscovitine |
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