Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice
To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain dev...
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| Veröffentlicht in: | Brain research 2000-09, Vol.876 (1), p.55-61 |
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| creator | Kask, Kalev Jerecic, Jasna Zamanillo, Daniel Wilbertz, Johannes Sprengel, Rolf H. Seeburg, Peter |
| description | To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of β-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons. |
| doi_str_mv | 10.1016/S0006-8993(00)02599-3 |
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In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(00)02599-3</identifier><identifier>PMID: 10973593</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Aging - metabolism ; Animals ; Animals, Newborn - growth & development ; Animals, Newborn - metabolism ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Cre recombinase ; Fundamental and applied biological sciences. Psychology ; Genes, Reporter - physiology ; High-affinity kainate receptor subunit ; Immunohistochemistry ; Integrases - genetics ; Integrases - metabolism ; Lac Operon - physiology ; lacZ reporter mice ; Mice ; Mice, Transgenic - genetics ; Protein Isoforms - metabolism ; Receptors, Kainic Acid - genetics ; Receptors, Kainic Acid - metabolism ; RNA, Messenger - metabolism ; Tissue Distribution ; Vertebrates: nervous system and sense organs ; Viral Proteins ; YAC transgenic</subject><ispartof>Brain research, 2000-09, Vol.876 (1), p.55-61</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-b28a71ff0ff48c6a12f60a916148efe6c7cbc064aacf3227f1c8be64f2fe26af3</citedby><cites>FETCH-LOGICAL-c459t-b28a71ff0ff48c6a12f60a916148efe6c7cbc064aacf3227f1c8be64f2fe26af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006899300025993$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1501939$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10973593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:16842997$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Kask, Kalev</creatorcontrib><creatorcontrib>Jerecic, Jasna</creatorcontrib><creatorcontrib>Zamanillo, Daniel</creatorcontrib><creatorcontrib>Wilbertz, Johannes</creatorcontrib><creatorcontrib>Sprengel, Rolf</creatorcontrib><creatorcontrib>H. Seeburg, Peter</creatorcontrib><title>Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of β-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Animals, Newborn - growth & development</subject><subject>Animals, Newborn - metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Cre recombinase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Reporter - physiology</subject><subject>High-affinity kainate receptor subunit</subject><subject>Immunohistochemistry</subject><subject>Integrases - genetics</subject><subject>Integrases - metabolism</subject><subject>Lac Operon - physiology</subject><subject>lacZ reporter mice</subject><subject>Mice</subject><subject>Mice, Transgenic - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>Receptors, Kainic Acid - genetics</subject><subject>Receptors, Kainic Acid - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Tissue Distribution</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Viral Proteins</subject><subject>YAC transgenic</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EosvCI4B8QAgOgbGdOPEJrRZoEZU4AAdOluMdV6ZJnNrJtn17vM2qcOvJ9uizZ_x_hLxk8J4Bkx9-AIAsGqXEW4B3wCulCvGIrFhT80LyEh6T1T1yQp6l9CcfhVDwlJwwULWolFiRi0-4xy6MPQ6T6egYg_Md0uDopfGDmZBGtDhOIdI0t_PgJ_ptw3Jxj6bDHW1v6TYixZsxYko-DNQP9PdmS6dohnSBg7e09xafkyfOdAlfHNc1-fXl88_tWXH-_fTrdnNe2LJSU9HyxtTMOXCubKw0jDsJRjHJygYdSlvb1oIsjbFOcF47ZpsWZem4Qy6NE2tSLO-maxznVo_R9ybe6mC8PpYu8w61BKhzHmvyZuHzz69mTJPufbLYdWbAMCddcy5YVYoHQVbLkgtoMlgtoI0hpYjufgYG-qBO36nTBy8aQN-p04cGr44N5rbH3X-3FlcZeH0ETLKmczlg69M_rgKmhMrYxwXDHPPeY9TJehws7nxWOeld8A9M8hfUQrbj</recordid><startdate>20000908</startdate><enddate>20000908</enddate><creator>Kask, Kalev</creator><creator>Jerecic, Jasna</creator><creator>Zamanillo, Daniel</creator><creator>Wilbertz, Johannes</creator><creator>Sprengel, Rolf</creator><creator>H. Seeburg, Peter</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20000908</creationdate><title>Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice</title><author>Kask, Kalev ; Jerecic, Jasna ; Zamanillo, Daniel ; Wilbertz, Johannes ; Sprengel, Rolf ; H. Seeburg, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-b28a71ff0ff48c6a12f60a916148efe6c7cbc064aacf3227f1c8be64f2fe26af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Animals, Newborn - growth & development</topic><topic>Animals, Newborn - metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Cre recombinase</topic><topic>Fundamental and applied biological sciences. 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During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>10973593</pmid><doi>10.1016/S0006-8993(00)02599-3</doi><tpages>7</tpages></addata></record> |
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| subjects | Aging - metabolism Animals Animals, Newborn - growth & development Animals, Newborn - metabolism Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Cre recombinase Fundamental and applied biological sciences. Psychology Genes, Reporter - physiology High-affinity kainate receptor subunit Immunohistochemistry Integrases - genetics Integrases - metabolism Lac Operon - physiology lacZ reporter mice Mice Mice, Transgenic - genetics Protein Isoforms - metabolism Receptors, Kainic Acid - genetics Receptors, Kainic Acid - metabolism RNA, Messenger - metabolism Tissue Distribution Vertebrates: nervous system and sense organs Viral Proteins YAC transgenic |
| title | Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice |
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