Effect of triclosan on interferon-γ production and major histocompatibility complex class II expression in human gingival fibroblasts

Background, aims: The effect of triclosan (2,4,4′‐trichloro‐2′‐hydroxyl‐diphenyl ether) on the production of interferon‐γ (IFN‐γ) and the expression of major histocompatibility complex (MHC) class II antigen was studied in human gingival fibroblasts isolated from 4 individuals. Methods/Results: AII...

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Veröffentlicht in:	Journal of clinical periodontology 2000-10, Vol.27 (10), p.733-737
Hauptverfasser:	Mustafa, Manal, Bakhiet, Moiz, Wondimu, Biniyam, Modéer, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Infective Agents, Local - pharmacology Cells, Cultured Child Dentistry Enzyme-Linked Immunosorbent Assay Fibroblasts - drug effects Fibroblasts - metabolism Gingiva - cytology Gingiva - drug effects Gingiva - metabolism gingival fibroblasts Histocompatibility Antigens Class II - biosynthesis Humans IFN-γ IFN‐γ, MHC class II Interferon-gamma - biosynthesis Medicin och hälsovetenskap MHC class II Phytohemagglutinins - pharmacology Triclosan - pharmacology
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container_title	Journal of clinical periodontology
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creator	Mustafa, Manal Bakhiet, Moiz Wondimu, Biniyam Modéer, Thomas
description	Background, aims: The effect of triclosan (2,4,4′‐trichloro‐2′‐hydroxyl‐diphenyl ether) on the production of interferon‐γ (IFN‐γ) and the expression of major histocompatibility complex (MHC) class II antigen was studied in human gingival fibroblasts isolated from 4 individuals. Methods/Results: AII cell lines demonstrated high IFN‐γ production in 24‐h cultures of human gingival fibroblasts stimulated by phytohemagglutinin (PHA) (5 μg/ml). Human gingival fibroblasts showed a high expression of MHC class II when stimulated with 500 and 1000 pg/ml rIFN‐γ in 7‐day cultures. Treatment of the cells with triclosan (0.5 μg/ml) reduced both IFN‐γ production and MHC class II expression in human gingival fibroblast cultures. Similar inhibitory effects on IFN‐γ production and MHC class II expression were observed when the anti‐inflammatory agent dexamethazone (1 μM) was used. Conclusion: The present study further supports the view that the agent has an anti‐inflammatory effect in addition to its antibacterial capacity.
doi_str_mv	10.1034/j.1600-051x.2000.027010733.x
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Methods/Results: AII cell lines demonstrated high IFN‐γ production in 24‐h cultures of human gingival fibroblasts stimulated by phytohemagglutinin (PHA) (5 μg/ml). Human gingival fibroblasts showed a high expression of MHC class II when stimulated with 500 and 1000 pg/ml rIFN‐γ in 7‐day cultures. Treatment of the cells with triclosan (0.5 μg/ml) reduced both IFN‐γ production and MHC class II expression in human gingival fibroblast cultures. Similar inhibitory effects on IFN‐γ production and MHC class II expression were observed when the anti‐inflammatory agent dexamethazone (1 μM) was used. 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Methods/Results: AII cell lines demonstrated high IFN‐γ production in 24‐h cultures of human gingival fibroblasts stimulated by phytohemagglutinin (PHA) (5 μg/ml). Human gingival fibroblasts showed a high expression of MHC class II when stimulated with 500 and 1000 pg/ml rIFN‐γ in 7‐day cultures. Treatment of the cells with triclosan (0.5 μg/ml) reduced both IFN‐γ production and MHC class II expression in human gingival fibroblast cultures. Similar inhibitory effects on IFN‐γ production and MHC class II expression were observed when the anti‐inflammatory agent dexamethazone (1 μM) was used. 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subjects	Anti-Infective Agents, Local - pharmacology Cells, Cultured Child Dentistry Enzyme-Linked Immunosorbent Assay Fibroblasts - drug effects Fibroblasts - metabolism Gingiva - cytology Gingiva - drug effects Gingiva - metabolism gingival fibroblasts Histocompatibility Antigens Class II - biosynthesis Humans IFN-γ IFN‐γ, MHC class II Interferon-gamma - biosynthesis Medicin och hälsovetenskap MHC class II Phytohemagglutinins - pharmacology Triclosan - pharmacology
title	Effect of triclosan on interferon-γ production and major histocompatibility complex class II expression in human gingival fibroblasts
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