Platelet-Leukocyte Cross Talk in Whole Blood
Abstract—Thrombosis and inflammation involve complex platelet-leukocyte interaction, the details of which are not fully elucidated. Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditionsat 37°C, with normal calcium concentr...
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| Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2000-12, Vol.20 (12), p.2702-2708 |
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| container_title | Arteriosclerosis, thrombosis, and vascular biology |
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| creator | Li, Nailin Hu, Hu Lindqvist, Malin Wikström-Jonsson, Eva Goodall, Alison H Hjemdahl, Paul |
| description | Abstract—Thrombosis and inflammation involve complex platelet-leukocyte interaction, the details of which are not fully elucidated. Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditionsat 37°C, with normal calcium concentrations, and with shear force. Platelet P-selectin and leukocyte CD11b expression were used to monitor platelet and leukocyte activation, respectively, and platelet-leukocyte aggregation (PLA) was analyzed. The leukocyte-specific agonist N-formyl-methionyl-leucyl-phenylalanine (10 mol/L) increased P-selectin–positive platelets from 2.5±0.1% to 5.1±0.6% (P |
| doi_str_mv | 10.1161/01.atv.20.12.2702 |
| format | Article |
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Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditionsat 37°C, with normal calcium concentrations, and with shear force. Platelet P-selectin and leukocyte CD11b expression were used to monitor platelet and leukocyte activation, respectively, and platelet-leukocyte aggregation (PLA) was analyzed. The leukocyte-specific agonist N-formyl-methionyl-leucyl-phenylalanine (10 mol/L) increased P-selectin–positive platelets from 2.5±0.1% to 5.1±0.6% (P <0.05). The increase was inhibited by either the platelet-activating factor (PAF) antagonist SR27417, the superoxide anion scavenger superoxide dismutase, the 5-lipoxygenase inhibitor Zileuton, or the 5-lipoxygenase–activating protein inhibitor MK-886, suggesting the involvement of PAF, superoxide anion, and 5-lipoxygenase products in leukocyte-induced platelet activation. The platelet-specific agonist collagen (1 μg/mL) increased leukocyte CD11b expression from 2.94±0.52 to 3.81±0.58 (P <0.05); this was not inhibited by the thromboxane A2 receptor antagonist ICI 192.605 or the PAF antagonist SR27417. Platelet P-selectin expression induced by N-formyl-methionyl-leucyl-phenylalanine and leukocyte CD11b expression induced by collagen could be suppressed by glycoprotein IIb/IIIa blockade or P-selectin blockade. This study documents platelet-leukocyte cross talk under conditions that mimic a physiological state and suggests that this involves multiple mediators and mechanisms. Furthermore, new evidence of integrin and selectin involvement in intracellular and intercellular signaling during platelet-leukocyte cross talk is provided.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/01.atv.20.12.2702</identifier><identifier>PMID: 11116075</identifier><identifier>CODEN: ATVBFA</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Adult ; Biological and medical sciences ; Blood coagulation. Blood cells ; Blood Platelets - physiology ; Blood Specimen Collection ; Cell Communication ; Collagen - antagonists & inhibitors ; Collagen - pharmacology ; Female ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Humans ; Leukocytes - physiology ; Macrophage-1 Antigen - analysis ; Macrophage-1 Antigen - biosynthesis ; Male ; Middle Aged ; Molecular and cellular biology ; Monocytes - drug effects ; N-Formylmethionine Leucyl-Phenylalanine - antagonists & inhibitors ; N-Formylmethionine Leucyl-Phenylalanine - pharmacology ; Neutrophils - drug effects ; P-Selectin - analysis ; P-Selectin - biosynthesis ; Platelet ; Platelet Activation ; Superoxide Dismutase - pharmacology</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2000-12, Vol.20 (12), p.2702-2708</ispartof><rights>2000 American Heart Association, Inc.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. Dec 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6279-a33dbfb474b4ea712d449c70e90e6b928f5d45d8ce723b37e60a54f9f2aa190c3</citedby><cites>FETCH-LOGICAL-c6279-a33dbfb474b4ea712d449c70e90e6b928f5d45d8ce723b37e60a54f9f2aa190c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=832630$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11116075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:17082125$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Nailin</creatorcontrib><creatorcontrib>Hu, Hu</creatorcontrib><creatorcontrib>Lindqvist, Malin</creatorcontrib><creatorcontrib>Wikström-Jonsson, Eva</creatorcontrib><creatorcontrib>Goodall, Alison H</creatorcontrib><creatorcontrib>Hjemdahl, Paul</creatorcontrib><title>Platelet-Leukocyte Cross Talk in Whole Blood</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>Abstract—Thrombosis and inflammation involve complex platelet-leukocyte interaction, the details of which are not fully elucidated. Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditionsat 37°C, with normal calcium concentrations, and with shear force. Platelet P-selectin and leukocyte CD11b expression were used to monitor platelet and leukocyte activation, respectively, and platelet-leukocyte aggregation (PLA) was analyzed. The leukocyte-specific agonist N-formyl-methionyl-leucyl-phenylalanine (10 mol/L) increased P-selectin–positive platelets from 2.5±0.1% to 5.1±0.6% (P <0.05). The increase was inhibited by either the platelet-activating factor (PAF) antagonist SR27417, the superoxide anion scavenger superoxide dismutase, the 5-lipoxygenase inhibitor Zileuton, or the 5-lipoxygenase–activating protein inhibitor MK-886, suggesting the involvement of PAF, superoxide anion, and 5-lipoxygenase products in leukocyte-induced platelet activation. The platelet-specific agonist collagen (1 μg/mL) increased leukocyte CD11b expression from 2.94±0.52 to 3.81±0.58 (P <0.05); this was not inhibited by the thromboxane A2 receptor antagonist ICI 192.605 or the PAF antagonist SR27417. Platelet P-selectin expression induced by N-formyl-methionyl-leucyl-phenylalanine and leukocyte CD11b expression induced by collagen could be suppressed by glycoprotein IIb/IIIa blockade or P-selectin blockade. This study documents platelet-leukocyte cross talk under conditions that mimic a physiological state and suggests that this involves multiple mediators and mechanisms. Furthermore, new evidence of integrin and selectin involvement in intracellular and intercellular signaling during platelet-leukocyte cross talk is provided.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - physiology</subject><subject>Blood Specimen Collection</subject><subject>Cell Communication</subject><subject>Collagen - antagonists & inhibitors</subject><subject>Collagen - pharmacology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Leukocytes - physiology</subject><subject>Macrophage-1 Antigen - analysis</subject><subject>Macrophage-1 Antigen - biosynthesis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular and cellular biology</subject><subject>Monocytes - drug effects</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - antagonists & inhibitors</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</subject><subject>Neutrophils - drug effects</subject><subject>P-Selectin - analysis</subject><subject>P-Selectin - biosynthesis</subject><subject>Platelet</subject><subject>Platelet Activation</subject><subject>Superoxide Dismutase - pharmacology</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1vEzEQhi0EoqXwA7igFUic2GCPv-JjifioFIkeAhwtr3dW2caJg71L1H9fr7IqUi3ZM7aed8Z-TchbRheMKfaZsoUb_i2gbGEBmsIzcskkiFoorp6XnGpTSyXggrzK-Y5SKgDoS3LBylBUy0vy6Ta4AQMO9RrHXfT3A1arFHOuNi7sqv5Q_dnGgNWXEGP7mrzoXMj4Zo5X5Ne3r5vVj3r98_vN6npdewWlo-O8bbpGaNEIdJpBK4TxmqKhqBoDy062QrZLjxp4wzUq6qToTAfOMUM9vyL1uW4-4XFs7DH1e5fubXS9nY92JUMrjdGMFf7jmT-m-HfEPNh9nz2G4A4Yx2w1lLsoqQr4_gl4F8d0KG-xULwxqrhSIHaG_ORDwu6xP6N28t1SZq83v4vEMrCT70Xzbi48Nnts_ytmowvwYQZc9i50yR18nx-5JQfFp9biTJ1iGDDlXRhPmOwWXRi2dvo_rqisoWRsWuoyueEPklCYMQ</recordid><startdate>200012</startdate><enddate>200012</enddate><creator>Li, Nailin</creator><creator>Hu, Hu</creator><creator>Lindqvist, Malin</creator><creator>Wikström-Jonsson, Eva</creator><creator>Goodall, Alison H</creator><creator>Hjemdahl, Paul</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>200012</creationdate><title>Platelet-Leukocyte Cross Talk in Whole Blood</title><author>Li, Nailin ; Hu, Hu ; Lindqvist, Malin ; Wikström-Jonsson, Eva ; Goodall, Alison H ; Hjemdahl, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6279-a33dbfb474b4ea712d449c70e90e6b928f5d45d8ce723b37e60a54f9f2aa190c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - physiology</topic><topic>Blood Specimen Collection</topic><topic>Cell Communication</topic><topic>Collagen - antagonists & inhibitors</topic><topic>Collagen - pharmacology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Leukocytes - physiology</topic><topic>Macrophage-1 Antigen - analysis</topic><topic>Macrophage-1 Antigen - biosynthesis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular and cellular biology</topic><topic>Monocytes - drug effects</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - antagonists & inhibitors</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</topic><topic>Neutrophils - drug effects</topic><topic>P-Selectin - analysis</topic><topic>P-Selectin - biosynthesis</topic><topic>Platelet</topic><topic>Platelet Activation</topic><topic>Superoxide Dismutase - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Nailin</creatorcontrib><creatorcontrib>Hu, Hu</creatorcontrib><creatorcontrib>Lindqvist, Malin</creatorcontrib><creatorcontrib>Wikström-Jonsson, Eva</creatorcontrib><creatorcontrib>Goodall, Alison H</creatorcontrib><creatorcontrib>Hjemdahl, Paul</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Nailin</au><au>Hu, Hu</au><au>Lindqvist, Malin</au><au>Wikström-Jonsson, Eva</au><au>Goodall, Alison H</au><au>Hjemdahl, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet-Leukocyte Cross Talk in Whole Blood</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2000-12</date><risdate>2000</risdate><volume>20</volume><issue>12</issue><spage>2702</spage><epage>2708</epage><pages>2702-2708</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><coden>ATVBFA</coden><abstract>Abstract—Thrombosis and inflammation involve complex platelet-leukocyte interaction, the details of which are not fully elucidated. Therefore, we investigated cross talk between platelets and leukocytes in whole blood, under the following physiological conditionsat 37°C, with normal calcium concentrations, and with shear force. Platelet P-selectin and leukocyte CD11b expression were used to monitor platelet and leukocyte activation, respectively, and platelet-leukocyte aggregation (PLA) was analyzed. The leukocyte-specific agonist N-formyl-methionyl-leucyl-phenylalanine (10 mol/L) increased P-selectin–positive platelets from 2.5±0.1% to 5.1±0.6% (P <0.05). The increase was inhibited by either the platelet-activating factor (PAF) antagonist SR27417, the superoxide anion scavenger superoxide dismutase, the 5-lipoxygenase inhibitor Zileuton, or the 5-lipoxygenase–activating protein inhibitor MK-886, suggesting the involvement of PAF, superoxide anion, and 5-lipoxygenase products in leukocyte-induced platelet activation. The platelet-specific agonist collagen (1 μg/mL) increased leukocyte CD11b expression from 2.94±0.52 to 3.81±0.58 (P <0.05); this was not inhibited by the thromboxane A2 receptor antagonist ICI 192.605 or the PAF antagonist SR27417. Platelet P-selectin expression induced by N-formyl-methionyl-leucyl-phenylalanine and leukocyte CD11b expression induced by collagen could be suppressed by glycoprotein IIb/IIIa blockade or P-selectin blockade. This study documents platelet-leukocyte cross talk under conditions that mimic a physiological state and suggests that this involves multiple mediators and mechanisms. Furthermore, new evidence of integrin and selectin involvement in intracellular and intercellular signaling during platelet-leukocyte cross talk is provided.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>11116075</pmid><doi>10.1161/01.atv.20.12.2702</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1079-5642 |
| ispartof | Arteriosclerosis, thrombosis, and vascular biology, 2000-12, Vol.20 (12), p.2702-2708 |
| issn | 1079-5642 1524-4636 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; Journals@Ovid Complete |
| subjects | Adult Biological and medical sciences Blood coagulation. Blood cells Blood Platelets - physiology Blood Specimen Collection Cell Communication Collagen - antagonists & inhibitors Collagen - pharmacology Female Flow Cytometry Fundamental and applied biological sciences. Psychology Humans Leukocytes - physiology Macrophage-1 Antigen - analysis Macrophage-1 Antigen - biosynthesis Male Middle Aged Molecular and cellular biology Monocytes - drug effects N-Formylmethionine Leucyl-Phenylalanine - antagonists & inhibitors N-Formylmethionine Leucyl-Phenylalanine - pharmacology Neutrophils - drug effects P-Selectin - analysis P-Selectin - biosynthesis Platelet Platelet Activation Superoxide Dismutase - pharmacology |
| title | Platelet-Leukocyte Cross Talk in Whole Blood |
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