C-terminally elongated form of PHI from porcine intestine
A C-terminally elongated form of peptide histidine isoleucine amide (PHI) was isolated from porcine intestine based on its effect on cAMP production in IMR-32 cells. The structure was determined by amino acid sequence analysis of tryptic fragments and by mass spectrometry. The peptide has 42 amino a...
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| Veröffentlicht in: | Cellular and molecular life sciences : CMLS 1999, Vol.56 (7/8), p.709-713 |
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| creator | Eriste, E Norberg, A Bonetto, V Nepomuceno, D Lovenberg, T.W Sillard, R Jornvall, H |
| description | A C-terminally elongated form of peptide histidine isoleucine amide (PHI) was isolated from porcine intestine based on its effect on cAMP production in IMR-32 cells. The structure was determined by amino acid sequence analysis of tryptic fragments and by mass spectrometry. The peptide has 42 amino acid residues like those described from human, rat and mouse, but the amino acid sequence of the C-terminal extension of pig PHI is unique. Unlike the other peptides, it has a C-terminal Ala and it differs at five positions from the human form and at six positions from the rat form, while the human and the rat forms differ by only two substitutions. To avoid confusion arising from different C-terminal residues, a unifying nomenclature is proposed: PHI-27 for the hormone and PHI-42 for the elongated product. |
| doi_str_mv | 10.1007/s000180050464 |
| format | Article |
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The structure was determined by amino acid sequence analysis of tryptic fragments and by mass spectrometry. The peptide has 42 amino acid residues like those described from human, rat and mouse, but the amino acid sequence of the C-terminal extension of pig PHI is unique. Unlike the other peptides, it has a C-terminal Ala and it differs at five positions from the human form and at six positions from the rat form, while the human and the rat forms differ by only two substitutions. To avoid confusion arising from different C-terminal residues, a unifying nomenclature is proposed: PHI-27 for the hormone and PHI-42 for the elongated product.</description><identifier>ISSN: 1420-682X</identifier><identifier>EISSN: 1420-9071</identifier><identifier>DOI: 10.1007/s000180050464</identifier><identifier>PMID: 11212317</identifier><language>eng</language><publisher>Switzerland: Springer Nature B.V</publisher><subject>Adenylyl Cyclases - metabolism ; Amino Acid Sequence ; amino acid sequences ; Amino acids ; Animals ; Cell Line ; Chromatography, High Pressure Liquid ; Cyclic AMP - metabolism ; Enzyme Activation - drug effects ; Hogs ; Hormones ; Humans ; intestines ; Intestines - chemistry ; Mass spectrometry ; Medicin och hälsovetenskap ; Molecular biology ; molecular conformation ; Molecular Sequence Data ; peptide histidine isoleucine amide ; Peptide PHI - chemistry ; Peptide PHI - isolation & purification ; Peptide PHI - metabolism ; Peptide PHI - pharmacology ; Peptides ; polypeptides ; purification ; Sequence Alignment ; Sequence Analysis, Protein ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Swine ; Trypsin - metabolism</subject><ispartof>Cellular and molecular life sciences : CMLS, 1999, Vol.56 (7/8), p.709-713</ispartof><rights>Birkhäuser Verlag Basel, 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-f5e2f39e26983a3d9b87fb401b2f8bf0a89b9f0cb247085cbd06660e39a41ae33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11147123/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11147123/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,552,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11212317$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1928455$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Eriste, E</creatorcontrib><creatorcontrib>Norberg, A</creatorcontrib><creatorcontrib>Bonetto, V</creatorcontrib><creatorcontrib>Nepomuceno, D</creatorcontrib><creatorcontrib>Lovenberg, T.W</creatorcontrib><creatorcontrib>Sillard, R</creatorcontrib><creatorcontrib>Jornvall, H</creatorcontrib><title>C-terminally elongated form of PHI from porcine intestine</title><title>Cellular and molecular life sciences : CMLS</title><addtitle>Cell Mol Life Sci</addtitle><description>A C-terminally elongated form of peptide histidine isoleucine amide (PHI) was isolated from porcine intestine based on its effect on cAMP production in IMR-32 cells. The structure was determined by amino acid sequence analysis of tryptic fragments and by mass spectrometry. The peptide has 42 amino acid residues like those described from human, rat and mouse, but the amino acid sequence of the C-terminal extension of pig PHI is unique. Unlike the other peptides, it has a C-terminal Ala and it differs at five positions from the human form and at six positions from the rat form, while the human and the rat forms differ by only two substitutions. To avoid confusion arising from different C-terminal residues, a unifying nomenclature is proposed: PHI-27 for the hormone and PHI-42 for the elongated product.</description><subject>Adenylyl Cyclases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cyclic AMP - metabolism</subject><subject>Enzyme Activation - drug effects</subject><subject>Hogs</subject><subject>Hormones</subject><subject>Humans</subject><subject>intestines</subject><subject>Intestines - chemistry</subject><subject>Mass spectrometry</subject><subject>Medicin och hälsovetenskap</subject><subject>Molecular biology</subject><subject>molecular conformation</subject><subject>Molecular Sequence Data</subject><subject>peptide histidine isoleucine amide</subject><subject>Peptide PHI - chemistry</subject><subject>Peptide PHI - isolation & purification</subject><subject>Peptide PHI - metabolism</subject><subject>Peptide PHI - pharmacology</subject><subject>Peptides</subject><subject>polypeptides</subject><subject>purification</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, Protein</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Swine</subject><subject>Trypsin - metabolism</subject><issn>1420-682X</issn><issn>1420-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>D8T</sourceid><recordid>eNp1kc1v1DAQxS1ERUvhyJVGPXALnfFX7FNVrYBWqtRKUImb5ST2kpLEWztbtP89rjawbCVOHnl-73k8j5B3CB8RoDpLAIAKQACX_AU5Qk6h1FDhy7mWin4_JK9Tus-gUFS-IoeIFCnD6ojoRTm5OHSj7ftN4fowLu3k2sKHOBTBF7eXV4WPYShWITbd6IpunFyacvWGHHjbJ_d2Po_J3edP3xaX5fXNl6vFxXXZCIpT6YWjnmlHpVbMslbXqvI1B6ypV7UHq3StPTQ15RUo0dQtSCnBMW05WsfYMSm3vumXW61rs4rdYOPGBNuZ-epnrpwRWmkpMl_9l1_F0O5Ef4SoqeLiSXm-Veb24NrGjVO0_b7BXmfsfphleDSIyKu80OzwYXaI4WGd92SGLjWu7-3owjoZqbnOT2EGT5-B92EdcwjJKMUlo1Tw3c-bGFKKzv8dBcE8hW_2ws_8-3_n39Fz2hk42QLeBmOXsUvm7isFZEA1p6zi7Dd5B7P7</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Eriste, E</creator><creator>Norberg, A</creator><creator>Bonetto, V</creator><creator>Nepomuceno, D</creator><creator>Lovenberg, T.W</creator><creator>Sillard, R</creator><creator>Jornvall, H</creator><general>Springer Nature B.V</general><general>Birkhäuser Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>1999</creationdate><title>C-terminally elongated form of PHI from porcine intestine</title><author>Eriste, E ; Norberg, A ; Bonetto, V ; Nepomuceno, D ; Lovenberg, T.W ; Sillard, R ; Jornvall, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-f5e2f39e26983a3d9b87fb401b2f8bf0a89b9f0cb247085cbd06660e39a41ae33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenylyl Cyclases - 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metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eriste, E</creatorcontrib><creatorcontrib>Norberg, A</creatorcontrib><creatorcontrib>Bonetto, V</creatorcontrib><creatorcontrib>Nepomuceno, D</creatorcontrib><creatorcontrib>Lovenberg, T.W</creatorcontrib><creatorcontrib>Sillard, R</creatorcontrib><creatorcontrib>Jornvall, H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Cellular and molecular life sciences : CMLS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eriste, E</au><au>Norberg, A</au><au>Bonetto, V</au><au>Nepomuceno, D</au><au>Lovenberg, T.W</au><au>Sillard, R</au><au>Jornvall, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C-terminally elongated form of PHI from porcine intestine</atitle><jtitle>Cellular and molecular life sciences : CMLS</jtitle><addtitle>Cell Mol Life Sci</addtitle><date>1999</date><risdate>1999</risdate><volume>56</volume><issue>7/8</issue><spage>709</spage><epage>713</epage><pages>709-713</pages><issn>1420-682X</issn><eissn>1420-9071</eissn><abstract>A C-terminally elongated form of peptide histidine isoleucine amide (PHI) was isolated from porcine intestine based on its effect on cAMP production in IMR-32 cells. The structure was determined by amino acid sequence analysis of tryptic fragments and by mass spectrometry. The peptide has 42 amino acid residues like those described from human, rat and mouse, but the amino acid sequence of the C-terminal extension of pig PHI is unique. Unlike the other peptides, it has a C-terminal Ala and it differs at five positions from the human form and at six positions from the rat form, while the human and the rat forms differ by only two substitutions. To avoid confusion arising from different C-terminal residues, a unifying nomenclature is proposed: PHI-27 for the hormone and PHI-42 for the elongated product.</abstract><cop>Switzerland</cop><pub>Springer Nature B.V</pub><pmid>11212317</pmid><doi>10.1007/s000180050464</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cellular and molecular life sciences : CMLS, 1999, Vol.56 (7/8), p.709-713 |
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| subjects | Adenylyl Cyclases - metabolism Amino Acid Sequence amino acid sequences Amino acids Animals Cell Line Chromatography, High Pressure Liquid Cyclic AMP - metabolism Enzyme Activation - drug effects Hogs Hormones Humans intestines Intestines - chemistry Mass spectrometry Medicin och hälsovetenskap Molecular biology molecular conformation Molecular Sequence Data peptide histidine isoleucine amide Peptide PHI - chemistry Peptide PHI - isolation & purification Peptide PHI - metabolism Peptide PHI - pharmacology Peptides polypeptides purification Sequence Alignment Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Swine Trypsin - metabolism |
| title | C-terminally elongated form of PHI from porcine intestine |
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