[17] S-Glutathionylation of glyceraldehyde-3-phosphate dehydrogenase: Role of thiol oxidation and catalysis by glutaredoxin

This chapter discusses methods employed to delineate the mechanism of S-glutathionylation of purified GAPDH, focusing on the roles of direct oxidation of the protein, with subsequent interaction of GSSG and the influence of GRX on this process. The chapter illustrates the complexity residing in the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Methods in Enzymology 2002, Vol.348, p.175-182
Hauptverfasser:	Cotgreave, Ian A., Gerdes, Robert, Schuppe-Koistinen, Ina, Lind, Christina
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal physiology Autoradiography Biochemistry Biophysics Catalysis Escherichia coli - enzymology Glutaredoxins Glutathione - chemistry Glutathione - metabolism Glutathione Disulfide - chemistry Glutathione Disulfide - metabolism Glyceraldehyde-3-Phosphate Dehydrogenases - chemistry Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Humans In Vitro Techniques Oxidation-Reduction Oxidoreductases Proteins - metabolism Recombinant Proteins - metabolism Sulfhydryl Compounds - chemistry Sulfhydryl Compounds - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!