Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture
Following injury, red blood cells (RBC) may interact with extracellular matrix (ECM). In the present study we hypothesised that RBC, and soluble factors from RBC, might mediate remodelling of ECM by affecting fibroblast-mediated contraction of three dimensional collagen gels. Human lung fibroblasts...
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| Veröffentlicht in: | Inflammation research 2002-05, Vol.51 (5), p.245-251 |
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| creator | Fredriksson, K Lundahl, J Fernvik, E Liu, X D Rennard, S I Sköld, C M |
| description | Following injury, red blood cells (RBC) may interact with extracellular matrix (ECM). In the present study we hypothesised that RBC, and soluble factors from RBC, might mediate remodelling of ECM by affecting fibroblast-mediated contraction of three dimensional collagen gels.
Human lung fibroblasts (HFL-1), were cultured together with isolated RBC, conditioned medium from RBC (RBC-CM) and hemolysed RBC in type I collagen gels. Gel contraction was determined by an image analyser.
Both RBC, RBC-CM and hemolysed RBC stimulated gel contraction by fibroblasts (P < 0.001), compared to fibroblasts alone. The RBC-CM stimulated (P < 0.01) gel contraction in a time and concentration dependent manner. A similar effect was observed when supernatant from hemolysed RBC was tested. The production of fibronectin was increased (P < 0.01) in the co-culture system, compared to fibroblasts cultured alone.
The present study shows that RBC can interact with mesenchymal cells in vitro. The ability of RBC to modulate fibroblast-mediated contraction in vitro, might therefore be an important mechanism regulating repair processes after injury. |
| doi_str_mv | 10.1007/PL00000300 |
| format | Article |
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Human lung fibroblasts (HFL-1), were cultured together with isolated RBC, conditioned medium from RBC (RBC-CM) and hemolysed RBC in type I collagen gels. Gel contraction was determined by an image analyser.
Both RBC, RBC-CM and hemolysed RBC stimulated gel contraction by fibroblasts (P < 0.001), compared to fibroblasts alone. The RBC-CM stimulated (P < 0.01) gel contraction in a time and concentration dependent manner. A similar effect was observed when supernatant from hemolysed RBC was tested. The production of fibronectin was increased (P < 0.01) in the co-culture system, compared to fibroblasts cultured alone.
The present study shows that RBC can interact with mesenchymal cells in vitro. The ability of RBC to modulate fibroblast-mediated contraction in vitro, might therefore be an important mechanism regulating repair processes after injury.</description><identifier>ISSN: 1023-3830</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/PL00000300</identifier><identifier>PMID: 12056512</identifier><language>eng</language><publisher>Switzerland: Springer Nature B.V</publisher><subject>Animals ; Coculture Techniques ; Collagen - chemistry ; Culture Media, Conditioned ; DNA - chemistry ; DNA - genetics ; Enzyme-Linked Immunosorbent Assay ; Erythrocytes - physiology ; Extracellular Matrix - physiology ; Fibroblasts - physiology ; Fibronectins - chemistry ; Gels ; Hemolysis ; Humans ; Papain - pharmacology ; Rats</subject><ispartof>Inflammation research, 2002-05, Vol.51 (5), p.245-251</ispartof><rights>Copyright Springer-Verlag 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-bbf71a6aee935be052d6e577402209f508a3a1ceb9306eb1075b7274df301e993</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12056512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1939608$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Fredriksson, K</creatorcontrib><creatorcontrib>Lundahl, J</creatorcontrib><creatorcontrib>Fernvik, E</creatorcontrib><creatorcontrib>Liu, X D</creatorcontrib><creatorcontrib>Rennard, S I</creatorcontrib><creatorcontrib>Sköld, C M</creatorcontrib><title>Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture</title><title>Inflammation research</title><addtitle>Inflamm Res</addtitle><description>Following injury, red blood cells (RBC) may interact with extracellular matrix (ECM). In the present study we hypothesised that RBC, and soluble factors from RBC, might mediate remodelling of ECM by affecting fibroblast-mediated contraction of three dimensional collagen gels.
Human lung fibroblasts (HFL-1), were cultured together with isolated RBC, conditioned medium from RBC (RBC-CM) and hemolysed RBC in type I collagen gels. Gel contraction was determined by an image analyser.
Both RBC, RBC-CM and hemolysed RBC stimulated gel contraction by fibroblasts (P < 0.001), compared to fibroblasts alone. The RBC-CM stimulated (P < 0.01) gel contraction in a time and concentration dependent manner. A similar effect was observed when supernatant from hemolysed RBC was tested. The production of fibronectin was increased (P < 0.01) in the co-culture system, compared to fibroblasts cultured alone.
The present study shows that RBC can interact with mesenchymal cells in vitro. 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In the present study we hypothesised that RBC, and soluble factors from RBC, might mediate remodelling of ECM by affecting fibroblast-mediated contraction of three dimensional collagen gels.
Human lung fibroblasts (HFL-1), were cultured together with isolated RBC, conditioned medium from RBC (RBC-CM) and hemolysed RBC in type I collagen gels. Gel contraction was determined by an image analyser.
Both RBC, RBC-CM and hemolysed RBC stimulated gel contraction by fibroblasts (P < 0.001), compared to fibroblasts alone. The RBC-CM stimulated (P < 0.01) gel contraction in a time and concentration dependent manner. A similar effect was observed when supernatant from hemolysed RBC was tested. The production of fibronectin was increased (P < 0.01) in the co-culture system, compared to fibroblasts cultured alone.
The present study shows that RBC can interact with mesenchymal cells in vitro. The ability of RBC to modulate fibroblast-mediated contraction in vitro, might therefore be an important mechanism regulating repair processes after injury.</abstract><cop>Switzerland</cop><pub>Springer Nature B.V</pub><pmid>12056512</pmid><doi>10.1007/PL00000300</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Coculture Techniques Collagen - chemistry Culture Media, Conditioned DNA - chemistry DNA - genetics Enzyme-Linked Immunosorbent Assay Erythrocytes - physiology Extracellular Matrix - physiology Fibroblasts - physiology Fibronectins - chemistry Gels Hemolysis Humans Papain - pharmacology Rats |
| title | Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture |
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