Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection
Human cytomegalovirus (HCMV) infects dendritic cells (DCs) in vitro and inhibits their maturation properties and their ability to stimulate T-cell proliferation and cytotoxicity. This study analyzed HCMV infection of DCs in vivo. We compared blood DCs and monocyte-derived DCs from heart-transplant p...
Gespeichert in:
| Veröffentlicht in: | Transplantation 2005-01, Vol.79 (2), p.219-227 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 227 |
|---|---|
| container_issue | 2 |
| container_start_page | 219 |
| container_title | Transplantation |
| container_volume | 79 |
| creator | VARANI, Stefania FRASCAROLI, Giada GIBELLINI, Davide POTENA, Luciano LAZZAROTTO, Tiziana LEMOLI, Roberto M MAGELLI, Carlo SÖDERBERG-NAUCLER, Cecilia LANDINI, Maria P |
| description | Human cytomegalovirus (HCMV) infects dendritic cells (DCs) in vitro and inhibits their maturation properties and their ability to stimulate T-cell proliferation and cytotoxicity. This study analyzed HCMV infection of DCs in vivo.
We compared blood DCs and monocyte-derived DCs from heart-transplant patients undergoing an acute HCMV infection with DCs obtained from HCMV-negative transplant patients. Diagnosis of active HCMV infection was established by antigenemia test.
We detected viral RNA and antigens in defined DC subsets obtained from patients undergoing an active HCMV infection. In addition, we found an impaired immunophenotype in immature DCs from HCMV-positive subjects and a reduced ability of mature DCs from the same group of patients to stimulate allogenic T-cell proliferation.
The impaired immunophenotype and function detected in DCs from transplant patients undergoing an active HCMV infection may be a mechanism used by the virus to interfere with early immune functions and thereby contributing to the HCMV-induced immunosuppression in these patients. |
| doi_str_mv | 10.1097/01.TP.0000147359.63158.29 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_582366</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17772293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-503587eda8d8e4085fb3eb532e8d82e7f0a1398b5711cfc9c8a1af5f5990dd6c3</originalsourceid><addsrcrecordid>eNqFkk2PFCEQhonRuOPoXzB40FuPVNM0zdFs_NhkE_cwngkD1bNoN90CvZs5-N9ld0bnZOQCqTxPQaiXkDfANsCUfM9gs73ZsLKgkVyoTctBdJtaPSErELypWtaxp2TFWAMVcC4vyIuUvhdecCmfkwsQbSukhBX5dTXOxkd01GFw0WdvqcVhoH4clzDNtximfJiRmuBovwSb_RSoD_QWTcw0RxPSPJiQ6Wyyx5ATXYLDuJ982FNT8Duk9pCnEfdmmO58XFLRe3xs9JI8682Q8NVpX5Nvnz5uL79U118_X11-uK6sAJ4rwbjoJDrTuQ4b1ol-x3EneI2lUKPsmQGuup2QALa3ynYGTC96oRRzrrV8Tapj33SP87LTc_SjiQc9Ga9PpR_lhFp0NW_bwst_8nOc3Fn6I4ISTAAU893RLNjPBVPWo08PH2oCTkvSreSyaZT8LwhSyrpWvIDqCNo4pRSx__saYPohDpqB3t7ocxz0Yxx0kdfk9emSZTeiO5un-Rfg7QkwyZqhL-O0Pp25tmkLqfhvjvHDoA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17772293</pqid></control><display><type>article</type><title>Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>VARANI, Stefania ; FRASCAROLI, Giada ; GIBELLINI, Davide ; POTENA, Luciano ; LAZZAROTTO, Tiziana ; LEMOLI, Roberto M ; MAGELLI, Carlo ; SÖDERBERG-NAUCLER, Cecilia ; LANDINI, Maria P</creator><creatorcontrib>VARANI, Stefania ; FRASCAROLI, Giada ; GIBELLINI, Davide ; POTENA, Luciano ; LAZZAROTTO, Tiziana ; LEMOLI, Roberto M ; MAGELLI, Carlo ; SÖDERBERG-NAUCLER, Cecilia ; LANDINI, Maria P</creatorcontrib><description>Human cytomegalovirus (HCMV) infects dendritic cells (DCs) in vitro and inhibits their maturation properties and their ability to stimulate T-cell proliferation and cytotoxicity. This study analyzed HCMV infection of DCs in vivo.
We compared blood DCs and monocyte-derived DCs from heart-transplant patients undergoing an acute HCMV infection with DCs obtained from HCMV-negative transplant patients. Diagnosis of active HCMV infection was established by antigenemia test.
We detected viral RNA and antigens in defined DC subsets obtained from patients undergoing an active HCMV infection. In addition, we found an impaired immunophenotype in immature DCs from HCMV-positive subjects and a reduced ability of mature DCs from the same group of patients to stimulate allogenic T-cell proliferation.
The impaired immunophenotype and function detected in DCs from transplant patients undergoing an active HCMV infection may be a mechanism used by the virus to interfere with early immune functions and thereby contributing to the HCMV-induced immunosuppression in these patients.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/01.TP.0000147359.63158.29</identifier><identifier>PMID: 15665771</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Antigens, CD - blood ; Antigens, Viral - isolation & purification ; Biological and medical sciences ; Cell Culture Techniques ; Cytomegalovirus Infections - immunology ; Dendritic Cells - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Graft Rejection - epidemiology ; Heart Transplantation - immunology ; Human cytomegalovirus ; Humans ; Immunophenotyping - methods ; Medical sciences ; Medicin och hälsovetenskap ; Middle Aged ; Monocytes - immunology ; Monocytes - virology ; Postoperative Complications - immunology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - blood ; RNA, Viral - isolation & purification ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tissue, organ and graft immunology</subject><ispartof>Transplantation, 2005-01, Vol.79 (2), p.219-227</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-503587eda8d8e4085fb3eb532e8d82e7f0a1398b5711cfc9c8a1af5f5990dd6c3</citedby><cites>FETCH-LOGICAL-c513t-503587eda8d8e4085fb3eb532e8d82e7f0a1398b5711cfc9c8a1af5f5990dd6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16466659$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15665771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1950511$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>VARANI, Stefania</creatorcontrib><creatorcontrib>FRASCAROLI, Giada</creatorcontrib><creatorcontrib>GIBELLINI, Davide</creatorcontrib><creatorcontrib>POTENA, Luciano</creatorcontrib><creatorcontrib>LAZZAROTTO, Tiziana</creatorcontrib><creatorcontrib>LEMOLI, Roberto M</creatorcontrib><creatorcontrib>MAGELLI, Carlo</creatorcontrib><creatorcontrib>SÖDERBERG-NAUCLER, Cecilia</creatorcontrib><creatorcontrib>LANDINI, Maria P</creatorcontrib><title>Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Human cytomegalovirus (HCMV) infects dendritic cells (DCs) in vitro and inhibits their maturation properties and their ability to stimulate T-cell proliferation and cytotoxicity. This study analyzed HCMV infection of DCs in vivo.
We compared blood DCs and monocyte-derived DCs from heart-transplant patients undergoing an acute HCMV infection with DCs obtained from HCMV-negative transplant patients. Diagnosis of active HCMV infection was established by antigenemia test.
We detected viral RNA and antigens in defined DC subsets obtained from patients undergoing an active HCMV infection. In addition, we found an impaired immunophenotype in immature DCs from HCMV-positive subjects and a reduced ability of mature DCs from the same group of patients to stimulate allogenic T-cell proliferation.
The impaired immunophenotype and function detected in DCs from transplant patients undergoing an active HCMV infection may be a mechanism used by the virus to interfere with early immune functions and thereby contributing to the HCMV-induced immunosuppression in these patients.</description><subject>Antigens, CD - blood</subject><subject>Antigens, Viral - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Cell Culture Techniques</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Dendritic Cells - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Graft Rejection - epidemiology</subject><subject>Heart Transplantation - immunology</subject><subject>Human cytomegalovirus</subject><subject>Humans</subject><subject>Immunophenotyping - methods</subject><subject>Medical sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Middle Aged</subject><subject>Monocytes - immunology</subject><subject>Monocytes - virology</subject><subject>Postoperative Complications - immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - blood</subject><subject>RNA, Viral - isolation & purification</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Tissue, organ and graft immunology</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk2PFCEQhonRuOPoXzB40FuPVNM0zdFs_NhkE_cwngkD1bNoN90CvZs5-N9ld0bnZOQCqTxPQaiXkDfANsCUfM9gs73ZsLKgkVyoTctBdJtaPSErELypWtaxp2TFWAMVcC4vyIuUvhdecCmfkwsQbSukhBX5dTXOxkd01GFw0WdvqcVhoH4clzDNtximfJiRmuBovwSb_RSoD_QWTcw0RxPSPJiQ6Wyyx5ATXYLDuJ982FNT8Duk9pCnEfdmmO58XFLRe3xs9JI8682Q8NVpX5Nvnz5uL79U118_X11-uK6sAJ4rwbjoJDrTuQ4b1ol-x3EneI2lUKPsmQGuup2QALa3ynYGTC96oRRzrrV8Tapj33SP87LTc_SjiQc9Ga9PpR_lhFp0NW_bwst_8nOc3Fn6I4ISTAAU893RLNjPBVPWo08PH2oCTkvSreSyaZT8LwhSyrpWvIDqCNo4pRSx__saYPohDpqB3t7ocxz0Yxx0kdfk9emSZTeiO5un-Rfg7QkwyZqhL-O0Pp25tmkLqfhvjvHDoA</recordid><startdate>20050127</startdate><enddate>20050127</enddate><creator>VARANI, Stefania</creator><creator>FRASCAROLI, Giada</creator><creator>GIBELLINI, Davide</creator><creator>POTENA, Luciano</creator><creator>LAZZAROTTO, Tiziana</creator><creator>LEMOLI, Roberto M</creator><creator>MAGELLI, Carlo</creator><creator>SÖDERBERG-NAUCLER, Cecilia</creator><creator>LANDINI, Maria P</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20050127</creationdate><title>Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection</title><author>VARANI, Stefania ; FRASCAROLI, Giada ; GIBELLINI, Davide ; POTENA, Luciano ; LAZZAROTTO, Tiziana ; LEMOLI, Roberto M ; MAGELLI, Carlo ; SÖDERBERG-NAUCLER, Cecilia ; LANDINI, Maria P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-503587eda8d8e4085fb3eb532e8d82e7f0a1398b5711cfc9c8a1af5f5990dd6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antigens, CD - blood</topic><topic>Antigens, Viral - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Cell Culture Techniques</topic><topic>Cytomegalovirus Infections - immunology</topic><topic>Dendritic Cells - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Graft Rejection - epidemiology</topic><topic>Heart Transplantation - immunology</topic><topic>Human cytomegalovirus</topic><topic>Humans</topic><topic>Immunophenotyping - methods</topic><topic>Medical sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Middle Aged</topic><topic>Monocytes - immunology</topic><topic>Monocytes - virology</topic><topic>Postoperative Complications - immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - blood</topic><topic>RNA, Viral - isolation & purification</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Tissue, organ and graft immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VARANI, Stefania</creatorcontrib><creatorcontrib>FRASCAROLI, Giada</creatorcontrib><creatorcontrib>GIBELLINI, Davide</creatorcontrib><creatorcontrib>POTENA, Luciano</creatorcontrib><creatorcontrib>LAZZAROTTO, Tiziana</creatorcontrib><creatorcontrib>LEMOLI, Roberto M</creatorcontrib><creatorcontrib>MAGELLI, Carlo</creatorcontrib><creatorcontrib>SÖDERBERG-NAUCLER, Cecilia</creatorcontrib><creatorcontrib>LANDINI, Maria P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VARANI, Stefania</au><au>FRASCAROLI, Giada</au><au>GIBELLINI, Davide</au><au>POTENA, Luciano</au><au>LAZZAROTTO, Tiziana</au><au>LEMOLI, Roberto M</au><au>MAGELLI, Carlo</au><au>SÖDERBERG-NAUCLER, Cecilia</au><au>LANDINI, Maria P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>2005-01-27</date><risdate>2005</risdate><volume>79</volume><issue>2</issue><spage>219</spage><epage>227</epage><pages>219-227</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Human cytomegalovirus (HCMV) infects dendritic cells (DCs) in vitro and inhibits their maturation properties and their ability to stimulate T-cell proliferation and cytotoxicity. This study analyzed HCMV infection of DCs in vivo.
We compared blood DCs and monocyte-derived DCs from heart-transplant patients undergoing an acute HCMV infection with DCs obtained from HCMV-negative transplant patients. Diagnosis of active HCMV infection was established by antigenemia test.
We detected viral RNA and antigens in defined DC subsets obtained from patients undergoing an active HCMV infection. In addition, we found an impaired immunophenotype in immature DCs from HCMV-positive subjects and a reduced ability of mature DCs from the same group of patients to stimulate allogenic T-cell proliferation.
The impaired immunophenotype and function detected in DCs from transplant patients undergoing an active HCMV infection may be a mechanism used by the virus to interfere with early immune functions and thereby contributing to the HCMV-induced immunosuppression in these patients.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>15665771</pmid><doi>10.1097/01.TP.0000147359.63158.29</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-1337 |
| ispartof | Transplantation, 2005-01, Vol.79 (2), p.219-227 |
| issn | 0041-1337 1534-6080 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_582366 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | Antigens, CD - blood Antigens, Viral - isolation & purification Biological and medical sciences Cell Culture Techniques Cytomegalovirus Infections - immunology Dendritic Cells - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Graft Rejection - epidemiology Heart Transplantation - immunology Human cytomegalovirus Humans Immunophenotyping - methods Medical sciences Medicin och hälsovetenskap Middle Aged Monocytes - immunology Monocytes - virology Postoperative Complications - immunology Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - blood RNA, Viral - isolation & purification Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tissue, organ and graft immunology |
| title | Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T16%3A19%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impaired%20dendritic%20cell%20immunophenotype%20and%20function%20in%20heart%20transplant%20patients%20undergoing%20active%20cytomegalovirus%20infection&rft.jtitle=Transplantation&rft.au=VARANI,%20Stefania&rft.date=2005-01-27&rft.volume=79&rft.issue=2&rft.spage=219&rft.epage=227&rft.pages=219-227&rft.issn=0041-1337&rft.eissn=1534-6080&rft.coden=TRPLAU&rft_id=info:doi/10.1097/01.TP.0000147359.63158.29&rft_dat=%3Cproquest_swepu%3E17772293%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17772293&rft_id=info:pmid/15665771&rfr_iscdi=true |