The C. elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell
The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases. Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis. C. elegan...
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| Veröffentlicht in: | Developmental biology 2005-11, Vol.287 (2), p.262-273 |
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| creator | Kagoshima, Hiroshi Sawa, Hitoshi Mitani, Shohei Bürglin, Thomas R. Shigesada, Katsuya Kohara, Yuji |
| description | The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases.
Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis.
C. elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1∷GFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages.
rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that
rnt-1 is allelic to
mab-2. Mutant analysis suggested that
rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Wnt signaling pathway. Expression studies of GFP∷POP-1 and TLP-1∷GFP reporters in
rnt-1/mab-2 mutants indicated that this gene functions upstream of
tlp-1 and downstream, or in parallel to,
pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/MAB-2 functions with POP-1 to control the asymmetry of the T cell division. |
| doi_str_mv | 10.1016/j.ydbio.2005.08.034 |
| format | Article |
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Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis.
C. elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1∷GFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages.
rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that
rnt-1 is allelic to
mab-2. Mutant analysis suggested that
rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Wnt signaling pathway. Expression studies of GFP∷POP-1 and TLP-1∷GFP reporters in
rnt-1/mab-2 mutants indicated that this gene functions upstream of
tlp-1 and downstream, or in parallel to,
pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/MAB-2 functions with POP-1 to control the asymmetry of the T cell division.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/j.ydbio.2005.08.034</identifier><identifier>PMID: 16226243</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Animals, Genetically Modified ; Asymmetrical cell division ; Caenorhabditis elegans ; Caenorhabditis elegans - embryology ; Caenorhabditis elegans - growth & development ; Caenorhabditis elegans - metabolism ; Caenorhabditis elegans Proteins - genetics ; Caenorhabditis elegans Proteins - metabolism ; Cell Division - physiology ; Cell Lineage ; Cell Polarity - physiology ; Disorders of Sex Development ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Gene Expression Regulation, Developmental ; High Mobility Group Proteins - genetics ; High Mobility Group Proteins - metabolism ; Larva ; mab-2 ; Medicin och hälsovetenskap ; Molecular Sequence Data ; Mutation ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; rnt-1 ; RUNX ; Seam cell ; Signal Transduction ; T blast cell ; T-Lymphocytes - physiology ; Tail - anatomy & histology ; Tail - embryology ; Tail - growth & development ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Wnt signal</subject><ispartof>Developmental biology, 2005-11, Vol.287 (2), p.262-273</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-425209222d05f9e8a52ddeec68c0b52f636dccb18b5cc9f5fe69fa1d294e74683</citedby><cites>FETCH-LOGICAL-c488t-425209222d05f9e8a52ddeec68c0b52f636dccb18b5cc9f5fe69fa1d294e74683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ydbio.2005.08.034$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16226243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1941360$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Kagoshima, Hiroshi</creatorcontrib><creatorcontrib>Sawa, Hitoshi</creatorcontrib><creatorcontrib>Mitani, Shohei</creatorcontrib><creatorcontrib>Bürglin, Thomas R.</creatorcontrib><creatorcontrib>Shigesada, Katsuya</creatorcontrib><creatorcontrib>Kohara, Yuji</creatorcontrib><title>The C. elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases.
Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis.
C. elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1∷GFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages.
rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that
rnt-1 is allelic to
mab-2. Mutant analysis suggested that
rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Wnt signaling pathway. Expression studies of GFP∷POP-1 and TLP-1∷GFP reporters in
rnt-1/mab-2 mutants indicated that this gene functions upstream of
tlp-1 and downstream, or in parallel to,
pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/MAB-2 functions with POP-1 to control the asymmetry of the T cell division.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Asymmetrical cell division</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - embryology</subject><subject>Caenorhabditis elegans - growth & development</subject><subject>Caenorhabditis elegans - metabolism</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>Cell Division - physiology</subject><subject>Cell Lineage</subject><subject>Cell Polarity - physiology</subject><subject>Disorders of Sex Development</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>High Mobility Group Proteins - genetics</subject><subject>High Mobility Group Proteins - metabolism</subject><subject>Larva</subject><subject>mab-2</subject><subject>Medicin och hälsovetenskap</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>rnt-1</subject><subject>RUNX</subject><subject>Seam cell</subject><subject>Signal Transduction</subject><subject>T blast cell</subject><subject>T-Lymphocytes - physiology</subject><subject>Tail - anatomy & histology</subject><subject>Tail - embryology</subject><subject>Tail - growth & development</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Wnt signal</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNp9kU9v1DAQxS0EotvCJ0BCPnFL6j-x1zlwKCsoSKVI1VbqzXLsMXhJ1ls7Kdpvj9Nd6AlOHnneb97YD6E3lNSUUHm-qfeuC7FmhIiaqJrw5hlaUNKKSsjm7jlaEEJZRSWRJ-g05w0hhCvFX6ITKhmTrOELlNc_AK9qDD18N9uMb26v7_CYSmlT2I0hbrE3dowJ31yvK3r-9eJDxXDIOMH9FBI47EvP5P0wwJiCNT220PfYhYeQZzp6PBaLNe56k8fH5iv0wps-w-vjeYZuP31crz5XV98uv6wurirbKDVWDROMtIwxR4RvQRnBnAOwUlnSCeYll87ajqpOWNt64UG23lDH2gaWjVT8DFWHufkX7KZO71IYTNrraII-Xv0sFWixVEIsi375T_0uRfcE_QFp21AuSSHfHcgiu58gj3oIeX6q2UKcspZKUcX5vBI_CG2KOSfwf00o0XOqeqMfU9VzqpooXVIt1Nvj-KkbwD0xxxiL4P1BAOU7HwIknW2ArQVXIrKjdjH81-A3peK1nw</recordid><startdate>20051115</startdate><enddate>20051115</enddate><creator>Kagoshima, Hiroshi</creator><creator>Sawa, Hitoshi</creator><creator>Mitani, Shohei</creator><creator>Bürglin, Thomas R.</creator><creator>Shigesada, Katsuya</creator><creator>Kohara, Yuji</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20051115</creationdate><title>The C. elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell</title><author>Kagoshima, Hiroshi ; Sawa, Hitoshi ; Mitani, Shohei ; Bürglin, Thomas R. ; Shigesada, Katsuya ; Kohara, Yuji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-425209222d05f9e8a52ddeec68c0b52f636dccb18b5cc9f5fe69fa1d294e74683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Asymmetrical cell division</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - embryology</topic><topic>Caenorhabditis elegans - growth & development</topic><topic>Caenorhabditis elegans - metabolism</topic><topic>Caenorhabditis elegans Proteins - genetics</topic><topic>Caenorhabditis elegans Proteins - metabolism</topic><topic>Cell Division - physiology</topic><topic>Cell Lineage</topic><topic>Cell Polarity - physiology</topic><topic>Disorders of Sex Development</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>High Mobility Group Proteins - genetics</topic><topic>High Mobility Group Proteins - metabolism</topic><topic>Larva</topic><topic>mab-2</topic><topic>Medicin och hälsovetenskap</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>rnt-1</topic><topic>RUNX</topic><topic>Seam cell</topic><topic>Signal Transduction</topic><topic>T blast cell</topic><topic>T-Lymphocytes - physiology</topic><topic>Tail - anatomy & histology</topic><topic>Tail - embryology</topic><topic>Tail - growth & development</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Wnt signal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kagoshima, Hiroshi</creatorcontrib><creatorcontrib>Sawa, Hitoshi</creatorcontrib><creatorcontrib>Mitani, Shohei</creatorcontrib><creatorcontrib>Bürglin, Thomas R.</creatorcontrib><creatorcontrib>Shigesada, Katsuya</creatorcontrib><creatorcontrib>Kohara, Yuji</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kagoshima, Hiroshi</au><au>Sawa, Hitoshi</au><au>Mitani, Shohei</au><au>Bürglin, Thomas R.</au><au>Shigesada, Katsuya</au><au>Kohara, Yuji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The C. elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2005-11-15</date><risdate>2005</risdate><volume>287</volume><issue>2</issue><spage>262</spage><epage>273</epage><pages>262-273</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases.
Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis.
C. elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1∷GFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages.
rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that
rnt-1 is allelic to
mab-2. Mutant analysis suggested that
rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Wnt signaling pathway. Expression studies of GFP∷POP-1 and TLP-1∷GFP reporters in
rnt-1/mab-2 mutants indicated that this gene functions upstream of
tlp-1 and downstream, or in parallel to,
pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/MAB-2 functions with POP-1 to control the asymmetry of the T cell division.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16226243</pmid><doi>10.1016/j.ydbio.2005.08.034</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals Animals, Genetically Modified Asymmetrical cell division Caenorhabditis elegans Caenorhabditis elegans - embryology Caenorhabditis elegans - growth & development Caenorhabditis elegans - metabolism Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism Cell Division - physiology Cell Lineage Cell Polarity - physiology Disorders of Sex Development DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene Expression Regulation, Developmental High Mobility Group Proteins - genetics High Mobility Group Proteins - metabolism Larva mab-2 Medicin och hälsovetenskap Molecular Sequence Data Mutation Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism rnt-1 RUNX Seam cell Signal Transduction T blast cell T-Lymphocytes - physiology Tail - anatomy & histology Tail - embryology Tail - growth & development Transcription Factors - genetics Transcription Factors - metabolism Wnt signal |
| title | The C. elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell |
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