Rad51-related changes in global gene expression
High expression of Rad51, the catalytic component in homologous recombination, has been reported to contribute to genomic instability. To elucidate biological processes related to Rad51, we performed global gene expression analysis on human fibrosarcoma cells induced to express variable Rad51 levels...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-03, Vol.341 (2), p.334-342 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Orre, Lukas M. Fält, Susann Szeles, Anna Lewensohn, Rolf Wennborg, Anders Flygare, Jenny |
| description | High expression of Rad51, the catalytic component in homologous recombination, has been reported to contribute to genomic instability. To elucidate biological processes related to Rad51, we performed global gene expression analysis on human fibrosarcoma cells induced to express variable Rad51 levels. The results indicate that Rad51 overexpression mediates late rather than early transcriptional responses. Using Gene Ontology analysis, we extracted functional annotations for Rad51-related changes in gene expression that were independent of general cell culture effects. High Rad51 levels conferred increased expression of genes involved in actin remodelling. These changes were accompanied by alterations in cell morphology. Moreover, core components of the mismatch repair (MMR) machinery were down-regulated in response to increased Rad51 expression. Given the role of MMR in the correction of DNA mismatches during replication and recombination, a concurrent increase in Rad51 levels and decrease in the expression of MMR genes could conceivably act synergistically towards genomic instability. |
| doi_str_mv | 10.1016/j.bbrc.2005.12.185 |
| format | Article |
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To elucidate biological processes related to Rad51, we performed global gene expression analysis on human fibrosarcoma cells induced to express variable Rad51 levels. The results indicate that Rad51 overexpression mediates late rather than early transcriptional responses. Using Gene Ontology analysis, we extracted functional annotations for Rad51-related changes in gene expression that were independent of general cell culture effects. High Rad51 levels conferred increased expression of genes involved in actin remodelling. These changes were accompanied by alterations in cell morphology. Moreover, core components of the mismatch repair (MMR) machinery were down-regulated in response to increased Rad51 expression. Given the role of MMR in the correction of DNA mismatches during replication and recombination, a concurrent increase in Rad51 levels and decrease in the expression of MMR genes could conceivably act synergistically towards genomic instability.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.12.185</identifier><identifier>PMID: 16427610</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actin remodelling ; Actins - metabolism ; Base Pair Mismatch ; Blotting, Western ; Cell Line ; Cell Line, Tumor ; Cells, Cultured ; DNA - metabolism ; DNA Damage ; DNA Repair ; DNA, Complementary - metabolism ; Gene Expression Regulation ; Genome ; Global gene expression ; Homologous recombination ; Humans ; Medicin och hälsovetenskap ; Microscopy, Fluorescence ; Mismatch repair ; Models, Biological ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; Oligonucleotide Probes - chemistry ; Rad51 ; Rad51 Recombinase - metabolism ; Rad51 Recombinase - physiology ; Recombination, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - metabolism ; RNA, Messenger - metabolism ; Time Factors ; Transcription, Genetic</subject><ispartof>Biochemical and biophysical research communications, 2006-03, Vol.341 (2), p.334-342</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-5609b8ba4b16d07b8943453dfc8078a7989273ffd64ef8677d1bc8bb89a61c3a3</citedby><cites>FETCH-LOGICAL-c471t-5609b8ba4b16d07b8943453dfc8078a7989273ffd64ef8677d1bc8bb89a61c3a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2005.12.185$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16427610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1932653$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Orre, Lukas M.</creatorcontrib><creatorcontrib>Fält, Susann</creatorcontrib><creatorcontrib>Szeles, Anna</creatorcontrib><creatorcontrib>Lewensohn, Rolf</creatorcontrib><creatorcontrib>Wennborg, Anders</creatorcontrib><creatorcontrib>Flygare, Jenny</creatorcontrib><title>Rad51-related changes in global gene expression</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>High expression of Rad51, the catalytic component in homologous recombination, has been reported to contribute to genomic instability. To elucidate biological processes related to Rad51, we performed global gene expression analysis on human fibrosarcoma cells induced to express variable Rad51 levels. The results indicate that Rad51 overexpression mediates late rather than early transcriptional responses. Using Gene Ontology analysis, we extracted functional annotations for Rad51-related changes in gene expression that were independent of general cell culture effects. High Rad51 levels conferred increased expression of genes involved in actin remodelling. These changes were accompanied by alterations in cell morphology. Moreover, core components of the mismatch repair (MMR) machinery were down-regulated in response to increased Rad51 expression. Given the role of MMR in the correction of DNA mismatches during replication and recombination, a concurrent increase in Rad51 levels and decrease in the expression of MMR genes could conceivably act synergistically towards genomic instability.</description><subject>Actin remodelling</subject><subject>Actins - metabolism</subject><subject>Base Pair Mismatch</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Cells, Cultured</subject><subject>DNA - metabolism</subject><subject>DNA Damage</subject><subject>DNA Repair</subject><subject>DNA, Complementary - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Genome</subject><subject>Global gene expression</subject><subject>Homologous recombination</subject><subject>Humans</subject><subject>Medicin och hälsovetenskap</subject><subject>Microscopy, Fluorescence</subject><subject>Mismatch repair</subject><subject>Models, Biological</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Oligonucleotide Probes - chemistry</subject><subject>Rad51</subject><subject>Rad51 Recombinase - metabolism</subject><subject>Rad51 Recombinase - physiology</subject><subject>Recombination, Genetic</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><subject>Transcription, Genetic</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEURi0EomngD7BAs2I303s9M35IbFBVHlIlJESl7iw_7gSHyUywkwL_vo4S6Iqy8pV1zqdrf4y9QmgQUFysG-eSbzhA3yBvUPVP2AJBQ80RuqdsAQCi5hpvz9h5zmsAxE7o5-wMRcelQFiwiy829FgnGu2OQuW_2WlFuYpTtRpnZ8dqRRNV9GubKOc4Ty_Ys8GOmV6eziW7eX_19fJjff35w6fLd9e17yTu6l6AdsrZzqEIIJ3SXdv1bRi8Aqms1Epz2Q5DEB0NSkgZ0HnlCmcF-ta2S1Yfc_NP2u6d2aa4sem3mW00p6vvZSLTS8lbKLz8J79Nc3iQ_oioWy76tphvjmbBfuwp78wmZk_jaCea99lIkJwrrv8LogQlobxyyfgR9GnOOdHwdxsEc2jOrM2hOXNoziA3pbkivT6l792GwoNyqqoAb48AlW-_i5RM9pEmTyEm8jsT5vhY_j2stql-</recordid><startdate>20060310</startdate><enddate>20060310</enddate><creator>Orre, Lukas M.</creator><creator>Fält, Susann</creator><creator>Szeles, Anna</creator><creator>Lewensohn, Rolf</creator><creator>Wennborg, Anders</creator><creator>Flygare, Jenny</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20060310</creationdate><title>Rad51-related changes in global gene expression</title><author>Orre, Lukas M. ; Fält, Susann ; Szeles, Anna ; Lewensohn, Rolf ; Wennborg, Anders ; Flygare, Jenny</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-5609b8ba4b16d07b8943453dfc8078a7989273ffd64ef8677d1bc8bb89a61c3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Actin remodelling</topic><topic>Actins - metabolism</topic><topic>Base Pair Mismatch</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Cells, Cultured</topic><topic>DNA - metabolism</topic><topic>DNA Damage</topic><topic>DNA Repair</topic><topic>DNA, Complementary - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Genome</topic><topic>Global gene expression</topic><topic>Homologous recombination</topic><topic>Humans</topic><topic>Medicin och hälsovetenskap</topic><topic>Microscopy, Fluorescence</topic><topic>Mismatch repair</topic><topic>Models, Biological</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Oligonucleotide Probes - chemistry</topic><topic>Rad51</topic><topic>Rad51 Recombinase - metabolism</topic><topic>Rad51 Recombinase - physiology</topic><topic>Recombination, Genetic</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orre, Lukas M.</creatorcontrib><creatorcontrib>Fält, Susann</creatorcontrib><creatorcontrib>Szeles, Anna</creatorcontrib><creatorcontrib>Lewensohn, Rolf</creatorcontrib><creatorcontrib>Wennborg, Anders</creatorcontrib><creatorcontrib>Flygare, Jenny</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orre, Lukas M.</au><au>Fält, Susann</au><au>Szeles, Anna</au><au>Lewensohn, Rolf</au><au>Wennborg, Anders</au><au>Flygare, Jenny</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rad51-related changes in global gene expression</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-03-10</date><risdate>2006</risdate><volume>341</volume><issue>2</issue><spage>334</spage><epage>342</epage><pages>334-342</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>High expression of Rad51, the catalytic component in homologous recombination, has been reported to contribute to genomic instability. To elucidate biological processes related to Rad51, we performed global gene expression analysis on human fibrosarcoma cells induced to express variable Rad51 levels. The results indicate that Rad51 overexpression mediates late rather than early transcriptional responses. Using Gene Ontology analysis, we extracted functional annotations for Rad51-related changes in gene expression that were independent of general cell culture effects. High Rad51 levels conferred increased expression of genes involved in actin remodelling. These changes were accompanied by alterations in cell morphology. Moreover, core components of the mismatch repair (MMR) machinery were down-regulated in response to increased Rad51 expression. Given the role of MMR in the correction of DNA mismatches during replication and recombination, a concurrent increase in Rad51 levels and decrease in the expression of MMR genes could conceivably act synergistically towards genomic instability.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16427610</pmid><doi>10.1016/j.bbrc.2005.12.185</doi><tpages>9</tpages></addata></record> |
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| subjects | Actin remodelling Actins - metabolism Base Pair Mismatch Blotting, Western Cell Line Cell Line, Tumor Cells, Cultured DNA - metabolism DNA Damage DNA Repair DNA, Complementary - metabolism Gene Expression Regulation Genome Global gene expression Homologous recombination Humans Medicin och hälsovetenskap Microscopy, Fluorescence Mismatch repair Models, Biological Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Oligonucleotide Probes - chemistry Rad51 Rad51 Recombinase - metabolism Rad51 Recombinase - physiology Recombination, Genetic Reverse Transcriptase Polymerase Chain Reaction RNA - metabolism RNA, Messenger - metabolism Time Factors Transcription, Genetic |
| title | Rad51-related changes in global gene expression |
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