Cartilage aggrecan undergoes significant compositional and structural alterations during laryngeal cancer

Aggrecan is a key component of cartilage and is responsible for the integrity and function of the tissue. In this study, the content of aggrecan and its structural modifications in adjacent to cancer apparently normal cartilages (AANCs) from various stages of laryngeal squamous cell carcinoma (LSCC)...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochimica et biophysica acta 2006-07, Vol.1760 (7), p.1046-1053
Hauptverfasser: Skandalis, Spyros S., Theocharis, Achilleas D., Vynios, Demitrios H., Papageorgakopoulou, Nickoletta, Hjerpe, Anders, Karamanos, Nikos K., Theocharis, Dimitrios A.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1053
container_issue 7
container_start_page 1046
container_title Biochimica et biophysica acta
container_volume 1760
creator Skandalis, Spyros S.
Theocharis, Achilleas D.
Vynios, Demitrios H.
Papageorgakopoulou, Nickoletta
Hjerpe, Anders
Karamanos, Nikos K.
Theocharis, Dimitrios A.
description Aggrecan is a key component of cartilage and is responsible for the integrity and function of the tissue. In this study, the content of aggrecan and its structural modifications in adjacent to cancer apparently normal cartilages (AANCs) from various stages of laryngeal squamous cell carcinoma (LSCC) were investigated. Our data demonstrated a stage-related loss of aggregable aggrecan in AANCs, compared to the healthy laryngeal cartilage (HLC), which was excessive in advanced stages of disease. On aggregable aggrecan level, AANCs were characterized by significant compositional and structural modifications, the extent of which was closely related with the stage of LSCC. Four concrete subpopulations of aggregable molecules with particular physicochemical characteristics were identified with a strong tendency to prevail subpopulations of molecules of lower hydrodynamic sizes with increasing LSCC stage. These findings demonstrated that the cleavage of aggregable aggrecan occurred in concrete peptide bonds within the CS-1 and CS-2 attachment domains. These significant alterations were closely associated with the process of cartilage destruction, indicating the crucial role of aggrecan during LSCC.
doi_str_mv 10.1016/j.bbagen.2006.02.007
format Article
fullrecord <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_576238</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304416506000389</els_id><sourcerecordid>68090449</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-b302cda37050c090870924f761cdf9e4544a4e1dd8cbacd931a3005f4975a44b3</originalsourceid><addsrcrecordid>eNp9UU2v1CAUJUbjG5_-A2O6ctd6KbS0GxMz8St5iRtdEwq3DWMHRqAa_7130onuZAOce87hcg9jLzk0HHj_5tRMk1kwNC1A30DbAKhH7MAH1dYDQY_ZAQTIWvK-u2PPcj4BrW7snrI7gpQUrTowfzSp-JWMKrMsCa0J1RYcpiVirrJfgp89gaWy8XyJ2Rcfg1krE1yVS9ps2dL1uhZM5lrLlduSD0u1mvQ7LEhFkltMz9mT2awZX9z2e_btw_uvx0_1w5ePn4_vHmorxqHUk4DWOiMUdGBhhEHB2MpZ9dy6eUTZSWkkcucGOxnrRsGNoG_NclSdkXIS96zeffMvvGyTviR_plZ0NF7foO90Qt2pvhUD8V_v_EuKPzbMRZ99triuJmDcsu4H6kLKkYhyJ9oUc044_7XmoK-R6JPeI9HXSDS0miIh2aub_zad0f0T3TIgwtudgDSVnx6TztYjjcx5yqNoF_3_X_gDcW2hmQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68090449</pqid></control><display><type>article</type><title>Cartilage aggrecan undergoes significant compositional and structural alterations during laryngeal cancer</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Skandalis, Spyros S. ; Theocharis, Achilleas D. ; Vynios, Demitrios H. ; Papageorgakopoulou, Nickoletta ; Hjerpe, Anders ; Karamanos, Nikos K. ; Theocharis, Dimitrios A.</creator><creatorcontrib>Skandalis, Spyros S. ; Theocharis, Achilleas D. ; Vynios, Demitrios H. ; Papageorgakopoulou, Nickoletta ; Hjerpe, Anders ; Karamanos, Nikos K. ; Theocharis, Dimitrios A.</creatorcontrib><description>Aggrecan is a key component of cartilage and is responsible for the integrity and function of the tissue. In this study, the content of aggrecan and its structural modifications in adjacent to cancer apparently normal cartilages (AANCs) from various stages of laryngeal squamous cell carcinoma (LSCC) were investigated. Our data demonstrated a stage-related loss of aggregable aggrecan in AANCs, compared to the healthy laryngeal cartilage (HLC), which was excessive in advanced stages of disease. On aggregable aggrecan level, AANCs were characterized by significant compositional and structural modifications, the extent of which was closely related with the stage of LSCC. Four concrete subpopulations of aggregable molecules with particular physicochemical characteristics were identified with a strong tendency to prevail subpopulations of molecules of lower hydrodynamic sizes with increasing LSCC stage. These findings demonstrated that the cleavage of aggregable aggrecan occurred in concrete peptide bonds within the CS-1 and CS-2 attachment domains. These significant alterations were closely associated with the process of cartilage destruction, indicating the crucial role of aggrecan during LSCC.</description><identifier>ISSN: 0304-4165</identifier><identifier>ISSN: 0006-3002</identifier><identifier>EISSN: 1872-8006</identifier><identifier>DOI: 10.1016/j.bbagen.2006.02.007</identifier><identifier>PMID: 16574327</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Aggrecan degradation ; Aggrecans ; Aggregable aggrecan ; Carcinoma, Squamous Cell - metabolism ; Cartilage ; Cartilage - metabolism ; Cartilage - pathology ; Chondroitin Sulfate Proteoglycans - biosynthesis ; Chondroitin Sulfate Proteoglycans - chemistry ; Extracellular Matrix Proteins - biosynthesis ; Extracellular Matrix Proteins - chemistry ; Gene Expression Regulation, Neoplastic ; Glycosaminoglycans - chemistry ; Humans ; Immunohistochemistry ; Laryngeal cancer ; Laryngeal Neoplasms - metabolism ; Lectins, C-Type - biosynthesis ; Lectins, C-Type - chemistry ; Male ; Middle Aged ; Peptides - chemistry ; Protein Binding ; Protein Structure, Tertiary ; Proteoglycans</subject><ispartof>Biochimica et biophysica acta, 2006-07, Vol.1760 (7), p.1046-1053</ispartof><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-b302cda37050c090870924f761cdf9e4544a4e1dd8cbacd931a3005f4975a44b3</citedby><cites>FETCH-LOGICAL-c398t-b302cda37050c090870924f761cdf9e4544a4e1dd8cbacd931a3005f4975a44b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304416506000389$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16574327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1947022$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Skandalis, Spyros S.</creatorcontrib><creatorcontrib>Theocharis, Achilleas D.</creatorcontrib><creatorcontrib>Vynios, Demitrios H.</creatorcontrib><creatorcontrib>Papageorgakopoulou, Nickoletta</creatorcontrib><creatorcontrib>Hjerpe, Anders</creatorcontrib><creatorcontrib>Karamanos, Nikos K.</creatorcontrib><creatorcontrib>Theocharis, Dimitrios A.</creatorcontrib><title>Cartilage aggrecan undergoes significant compositional and structural alterations during laryngeal cancer</title><title>Biochimica et biophysica acta</title><addtitle>Biochim Biophys Acta</addtitle><description>Aggrecan is a key component of cartilage and is responsible for the integrity and function of the tissue. In this study, the content of aggrecan and its structural modifications in adjacent to cancer apparently normal cartilages (AANCs) from various stages of laryngeal squamous cell carcinoma (LSCC) were investigated. Our data demonstrated a stage-related loss of aggregable aggrecan in AANCs, compared to the healthy laryngeal cartilage (HLC), which was excessive in advanced stages of disease. On aggregable aggrecan level, AANCs were characterized by significant compositional and structural modifications, the extent of which was closely related with the stage of LSCC. Four concrete subpopulations of aggregable molecules with particular physicochemical characteristics were identified with a strong tendency to prevail subpopulations of molecules of lower hydrodynamic sizes with increasing LSCC stage. These findings demonstrated that the cleavage of aggregable aggrecan occurred in concrete peptide bonds within the CS-1 and CS-2 attachment domains. These significant alterations were closely associated with the process of cartilage destruction, indicating the crucial role of aggrecan during LSCC.</description><subject>Adult</subject><subject>Aggrecan degradation</subject><subject>Aggrecans</subject><subject>Aggregable aggrecan</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Cartilage</subject><subject>Cartilage - metabolism</subject><subject>Cartilage - pathology</subject><subject>Chondroitin Sulfate Proteoglycans - biosynthesis</subject><subject>Chondroitin Sulfate Proteoglycans - chemistry</subject><subject>Extracellular Matrix Proteins - biosynthesis</subject><subject>Extracellular Matrix Proteins - chemistry</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Glycosaminoglycans - chemistry</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Laryngeal cancer</subject><subject>Laryngeal Neoplasms - metabolism</subject><subject>Lectins, C-Type - biosynthesis</subject><subject>Lectins, C-Type - chemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Peptides - chemistry</subject><subject>Protein Binding</subject><subject>Protein Structure, Tertiary</subject><subject>Proteoglycans</subject><issn>0304-4165</issn><issn>0006-3002</issn><issn>1872-8006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU2v1CAUJUbjG5_-A2O6ctd6KbS0GxMz8St5iRtdEwq3DWMHRqAa_7130onuZAOce87hcg9jLzk0HHj_5tRMk1kwNC1A30DbAKhH7MAH1dYDQY_ZAQTIWvK-u2PPcj4BrW7snrI7gpQUrTowfzSp-JWMKrMsCa0J1RYcpiVirrJfgp89gaWy8XyJ2Rcfg1krE1yVS9ps2dL1uhZM5lrLlduSD0u1mvQ7LEhFkltMz9mT2awZX9z2e_btw_uvx0_1w5ePn4_vHmorxqHUk4DWOiMUdGBhhEHB2MpZ9dy6eUTZSWkkcucGOxnrRsGNoG_NclSdkXIS96zeffMvvGyTviR_plZ0NF7foO90Qt2pvhUD8V_v_EuKPzbMRZ99triuJmDcsu4H6kLKkYhyJ9oUc044_7XmoK-R6JPeI9HXSDS0miIh2aub_zad0f0T3TIgwtudgDSVnx6TztYjjcx5yqNoF_3_X_gDcW2hmQ</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Skandalis, Spyros S.</creator><creator>Theocharis, Achilleas D.</creator><creator>Vynios, Demitrios H.</creator><creator>Papageorgakopoulou, Nickoletta</creator><creator>Hjerpe, Anders</creator><creator>Karamanos, Nikos K.</creator><creator>Theocharis, Dimitrios A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20060701</creationdate><title>Cartilage aggrecan undergoes significant compositional and structural alterations during laryngeal cancer</title><author>Skandalis, Spyros S. ; Theocharis, Achilleas D. ; Vynios, Demitrios H. ; Papageorgakopoulou, Nickoletta ; Hjerpe, Anders ; Karamanos, Nikos K. ; Theocharis, Dimitrios A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-b302cda37050c090870924f761cdf9e4544a4e1dd8cbacd931a3005f4975a44b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Aggrecan degradation</topic><topic>Aggrecans</topic><topic>Aggregable aggrecan</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Cartilage</topic><topic>Cartilage - metabolism</topic><topic>Cartilage - pathology</topic><topic>Chondroitin Sulfate Proteoglycans - biosynthesis</topic><topic>Chondroitin Sulfate Proteoglycans - chemistry</topic><topic>Extracellular Matrix Proteins - biosynthesis</topic><topic>Extracellular Matrix Proteins - chemistry</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Glycosaminoglycans - chemistry</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Laryngeal cancer</topic><topic>Laryngeal Neoplasms - metabolism</topic><topic>Lectins, C-Type - biosynthesis</topic><topic>Lectins, C-Type - chemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Peptides - chemistry</topic><topic>Protein Binding</topic><topic>Protein Structure, Tertiary</topic><topic>Proteoglycans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skandalis, Spyros S.</creatorcontrib><creatorcontrib>Theocharis, Achilleas D.</creatorcontrib><creatorcontrib>Vynios, Demitrios H.</creatorcontrib><creatorcontrib>Papageorgakopoulou, Nickoletta</creatorcontrib><creatorcontrib>Hjerpe, Anders</creatorcontrib><creatorcontrib>Karamanos, Nikos K.</creatorcontrib><creatorcontrib>Theocharis, Dimitrios A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Biochimica et biophysica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skandalis, Spyros S.</au><au>Theocharis, Achilleas D.</au><au>Vynios, Demitrios H.</au><au>Papageorgakopoulou, Nickoletta</au><au>Hjerpe, Anders</au><au>Karamanos, Nikos K.</au><au>Theocharis, Dimitrios A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cartilage aggrecan undergoes significant compositional and structural alterations during laryngeal cancer</atitle><jtitle>Biochimica et biophysica acta</jtitle><addtitle>Biochim Biophys Acta</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>1760</volume><issue>7</issue><spage>1046</spage><epage>1053</epage><pages>1046-1053</pages><issn>0304-4165</issn><issn>0006-3002</issn><eissn>1872-8006</eissn><abstract>Aggrecan is a key component of cartilage and is responsible for the integrity and function of the tissue. In this study, the content of aggrecan and its structural modifications in adjacent to cancer apparently normal cartilages (AANCs) from various stages of laryngeal squamous cell carcinoma (LSCC) were investigated. Our data demonstrated a stage-related loss of aggregable aggrecan in AANCs, compared to the healthy laryngeal cartilage (HLC), which was excessive in advanced stages of disease. On aggregable aggrecan level, AANCs were characterized by significant compositional and structural modifications, the extent of which was closely related with the stage of LSCC. Four concrete subpopulations of aggregable molecules with particular physicochemical characteristics were identified with a strong tendency to prevail subpopulations of molecules of lower hydrodynamic sizes with increasing LSCC stage. These findings demonstrated that the cleavage of aggregable aggrecan occurred in concrete peptide bonds within the CS-1 and CS-2 attachment domains. These significant alterations were closely associated with the process of cartilage destruction, indicating the crucial role of aggrecan during LSCC.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16574327</pmid><doi>10.1016/j.bbagen.2006.02.007</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0304-4165
ispartof Biochimica et biophysica acta, 2006-07, Vol.1760 (7), p.1046-1053
issn 0304-4165
0006-3002
1872-8006
language eng
recordid cdi_swepub_primary_oai_swepub_ki_se_576238
source MEDLINE; Elsevier ScienceDirect Journals
subjects Adult
Aggrecan degradation
Aggrecans
Aggregable aggrecan
Carcinoma, Squamous Cell - metabolism
Cartilage
Cartilage - metabolism
Cartilage - pathology
Chondroitin Sulfate Proteoglycans - biosynthesis
Chondroitin Sulfate Proteoglycans - chemistry
Extracellular Matrix Proteins - biosynthesis
Extracellular Matrix Proteins - chemistry
Gene Expression Regulation, Neoplastic
Glycosaminoglycans - chemistry
Humans
Immunohistochemistry
Laryngeal cancer
Laryngeal Neoplasms - metabolism
Lectins, C-Type - biosynthesis
Lectins, C-Type - chemistry
Male
Middle Aged
Peptides - chemistry
Protein Binding
Protein Structure, Tertiary
Proteoglycans
title Cartilage aggrecan undergoes significant compositional and structural alterations during laryngeal cancer
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T16%3A45%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cartilage%20aggrecan%20undergoes%20significant%20compositional%20and%20structural%20alterations%20during%20laryngeal%20cancer&rft.jtitle=Biochimica%20et%20biophysica%20acta&rft.au=Skandalis,%20Spyros%20S.&rft.date=2006-07-01&rft.volume=1760&rft.issue=7&rft.spage=1046&rft.epage=1053&rft.pages=1046-1053&rft.issn=0304-4165&rft.eissn=1872-8006&rft_id=info:doi/10.1016/j.bbagen.2006.02.007&rft_dat=%3Cproquest_swepu%3E68090449%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68090449&rft_id=info:pmid/16574327&rft_els_id=S0304416506000389&rfr_iscdi=true