Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic β-cells
Pancreatic β-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca 2+ from intracellular stores in β-cells but the identity of the channels involved in the Ca 2+ release has not been elucidated. We studi...
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| Veröffentlicht in: | Cell calcium (Edinburgh) 2006-06, Vol.39 (6), p.529-537 |
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| creator | Woolcott, Orison O. Gustafsson, Amanda J. Dzabic, Mensur Pierro, Cristina Tedeschi, Patrizia Sandgren, Johanna Bari, M. Rizuanul Hoa, Nguyen K. Bianchi, Marta Rakonjac, Marija Rådmark, Olof Östenson, Claes-Göran Islam, Md. Shahidul |
| description | Pancreatic β-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca
2+ from intracellular stores in β-cells but the identity of the channels involved in the Ca
2+ release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca
2+ concentration changes in pancreatic β-cells. Cytosolic free Ca
2+ concentration was measured in fura-2-loaded INS-1E cells and in primary β-cells from Wistar rats. The increase of cytosolic Ca
2+ concentration induced by arachidonic acid (150
μM) was due to both Ca
2+ release from intracellular stores and influx of Ca
2+ from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca
2+ increase. The Ca
2+ release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50
μM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca
2+ release by arachidonic acid. However, ryanodine (100
μM), a blocker of ryanodine receptors, abolished the effect of arachidonic acid on Ca
2+ release in both types of cells. These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in β-cells. |
| doi_str_mv | 10.1016/j.ceca.2006.02.003 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_576041</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0143416006000340</els_id><sourcerecordid>67967677</sourcerecordid><originalsourceid>FETCH-LOGICAL-c460t-b6de252540a7b1ec446dbf0fa0fddf95239b6df18a3282ec716a5fe9d706439d3</originalsourceid><addsrcrecordid>eNqFkc9u1DAQhy1ERZfCC3BAOXFq0rGd2GuJy6otf6RKXIAjlmNPul6ycbCTon0tHoRnwtGu4AScPBp_89PYHyEvKFQUqLjaVRatqRiAqIBVAPwRWdGGs5IqRR-TFdCalzUVcE6eprQDAMUlfULOqRAMlKhX5MsmGrv1LgzeFsZ6V_hUmGLcHpIPfbj31vS5P_kHM4VYhK6YtljEgxmC80Ou0OK43PihGM1gI5opJ_38UVrs-_SMnHWmT_j8dF6QT29uP16_K-8-vH1_vbkrbS1gKlvhkDWsqcHIlqKta-HaDjoDnXOdahhXGeno2nC2ZmglFabpUDkJoubK8QtSHnPTdxznVo_R70086GC8PrW-5gp1IwXUNPOXf-Vv_OeNDvFez7OmnFG-zvirIz7G8G3GNOm9T8sDzYBhTlpIJaSQ8r8glXQNSrEMsiNoY0gpYvd7BQp6sat3erGrF7samM5289DLU_rc7tH9GTnpzMDrI4D5rx88Rp2sx8Gi81nUpF3w_8r_BShFt_E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17180992</pqid></control><display><type>article</type><title>Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic β-cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Woolcott, Orison O. ; Gustafsson, Amanda J. ; Dzabic, Mensur ; Pierro, Cristina ; Tedeschi, Patrizia ; Sandgren, Johanna ; Bari, M. Rizuanul ; Hoa, Nguyen K. ; Bianchi, Marta ; Rakonjac, Marija ; Rådmark, Olof ; Östenson, Claes-Göran ; Islam, Md. Shahidul</creator><creatorcontrib>Woolcott, Orison O. ; Gustafsson, Amanda J. ; Dzabic, Mensur ; Pierro, Cristina ; Tedeschi, Patrizia ; Sandgren, Johanna ; Bari, M. Rizuanul ; Hoa, Nguyen K. ; Bianchi, Marta ; Rakonjac, Marija ; Rådmark, Olof ; Östenson, Claes-Göran ; Islam, Md. Shahidul</creatorcontrib><description>Pancreatic β-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca
2+ from intracellular stores in β-cells but the identity of the channels involved in the Ca
2+ release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca
2+ concentration changes in pancreatic β-cells. Cytosolic free Ca
2+ concentration was measured in fura-2-loaded INS-1E cells and in primary β-cells from Wistar rats. The increase of cytosolic Ca
2+ concentration induced by arachidonic acid (150
μM) was due to both Ca
2+ release from intracellular stores and influx of Ca
2+ from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca
2+ increase. The Ca
2+ release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50
μM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca
2+ release by arachidonic acid. However, ryanodine (100
μM), a blocker of ryanodine receptors, abolished the effect of arachidonic acid on Ca
2+ release in both types of cells. These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in β-cells.</description><identifier>ISSN: 0143-4160</identifier><identifier>ISSN: 1532-1991</identifier><identifier>EISSN: 1532-1991</identifier><identifier>DOI: 10.1016/j.ceca.2006.02.003</identifier><identifier>PMID: 16620964</identifier><language>eng</language><publisher>Netherlands: Elsevier India Pvt Ltd</publisher><subject>Animals ; Arachidonic acid ; Arachidonic Acid - pharmacology ; Calcium channels ; Calcium Channels, L-Type - metabolism ; Calcium Signaling - drug effects ; Calcium signalling ; Cells, Cultured ; Endoplasmic Reticulum - secretion ; Inositol 1,4,5-Trisphosphate Receptors - metabolism ; Insulin-Secreting Cells - drug effects ; Insulin-Secreting Cells - metabolism ; Male ; Nimodipine - pharmacology ; Nitric Oxide - metabolism ; Pancreatic β-cells ; Rats ; Rats, Wistar ; Ryanodine Receptor Calcium Release Channel - metabolism ; Ryanodine receptors</subject><ispartof>Cell calcium (Edinburgh), 2006-06, Vol.39 (6), p.529-537</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-b6de252540a7b1ec446dbf0fa0fddf95239b6df18a3282ec716a5fe9d706439d3</citedby><cites>FETCH-LOGICAL-c460t-b6de252540a7b1ec446dbf0fa0fddf95239b6df18a3282ec716a5fe9d706439d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ceca.2006.02.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16620964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-132138$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1950415$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Woolcott, Orison O.</creatorcontrib><creatorcontrib>Gustafsson, Amanda J.</creatorcontrib><creatorcontrib>Dzabic, Mensur</creatorcontrib><creatorcontrib>Pierro, Cristina</creatorcontrib><creatorcontrib>Tedeschi, Patrizia</creatorcontrib><creatorcontrib>Sandgren, Johanna</creatorcontrib><creatorcontrib>Bari, M. Rizuanul</creatorcontrib><creatorcontrib>Hoa, Nguyen K.</creatorcontrib><creatorcontrib>Bianchi, Marta</creatorcontrib><creatorcontrib>Rakonjac, Marija</creatorcontrib><creatorcontrib>Rådmark, Olof</creatorcontrib><creatorcontrib>Östenson, Claes-Göran</creatorcontrib><creatorcontrib>Islam, Md. Shahidul</creatorcontrib><title>Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic β-cells</title><title>Cell calcium (Edinburgh)</title><addtitle>Cell Calcium</addtitle><description>Pancreatic β-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca
2+ from intracellular stores in β-cells but the identity of the channels involved in the Ca
2+ release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca
2+ concentration changes in pancreatic β-cells. Cytosolic free Ca
2+ concentration was measured in fura-2-loaded INS-1E cells and in primary β-cells from Wistar rats. The increase of cytosolic Ca
2+ concentration induced by arachidonic acid (150
μM) was due to both Ca
2+ release from intracellular stores and influx of Ca
2+ from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca
2+ increase. The Ca
2+ release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50
μM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca
2+ release by arachidonic acid. However, ryanodine (100
μM), a blocker of ryanodine receptors, abolished the effect of arachidonic acid on Ca
2+ release in both types of cells. These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in β-cells.</description><subject>Animals</subject><subject>Arachidonic acid</subject><subject>Arachidonic Acid - pharmacology</subject><subject>Calcium channels</subject><subject>Calcium Channels, L-Type - metabolism</subject><subject>Calcium Signaling - drug effects</subject><subject>Calcium signalling</subject><subject>Cells, Cultured</subject><subject>Endoplasmic Reticulum - secretion</subject><subject>Inositol 1,4,5-Trisphosphate Receptors - metabolism</subject><subject>Insulin-Secreting Cells - drug effects</subject><subject>Insulin-Secreting Cells - metabolism</subject><subject>Male</subject><subject>Nimodipine - pharmacology</subject><subject>Nitric Oxide - metabolism</subject><subject>Pancreatic β-cells</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Ryanodine Receptor Calcium Release Channel - metabolism</subject><subject>Ryanodine receptors</subject><issn>0143-4160</issn><issn>1532-1991</issn><issn>1532-1991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQhy1ERZfCC3BAOXFq0rGd2GuJy6otf6RKXIAjlmNPul6ycbCTon0tHoRnwtGu4AScPBp_89PYHyEvKFQUqLjaVRatqRiAqIBVAPwRWdGGs5IqRR-TFdCalzUVcE6eprQDAMUlfULOqRAMlKhX5MsmGrv1LgzeFsZ6V_hUmGLcHpIPfbj31vS5P_kHM4VYhK6YtljEgxmC80Ou0OK43PihGM1gI5opJ_38UVrs-_SMnHWmT_j8dF6QT29uP16_K-8-vH1_vbkrbS1gKlvhkDWsqcHIlqKta-HaDjoDnXOdahhXGeno2nC2ZmglFabpUDkJoubK8QtSHnPTdxznVo_R70086GC8PrW-5gp1IwXUNPOXf-Vv_OeNDvFez7OmnFG-zvirIz7G8G3GNOm9T8sDzYBhTlpIJaSQ8r8glXQNSrEMsiNoY0gpYvd7BQp6sat3erGrF7samM5289DLU_rc7tH9GTnpzMDrI4D5rx88Rp2sx8Gi81nUpF3w_8r_BShFt_E</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Woolcott, Orison O.</creator><creator>Gustafsson, Amanda J.</creator><creator>Dzabic, Mensur</creator><creator>Pierro, Cristina</creator><creator>Tedeschi, Patrizia</creator><creator>Sandgren, Johanna</creator><creator>Bari, M. Rizuanul</creator><creator>Hoa, Nguyen K.</creator><creator>Bianchi, Marta</creator><creator>Rakonjac, Marija</creator><creator>Rådmark, Olof</creator><creator>Östenson, Claes-Göran</creator><creator>Islam, Md. Shahidul</creator><general>Elsevier India Pvt Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DF2</scope></search><sort><creationdate>20060601</creationdate><title>Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic β-cells</title><author>Woolcott, Orison O. ; Gustafsson, Amanda J. ; Dzabic, Mensur ; Pierro, Cristina ; Tedeschi, Patrizia ; Sandgren, Johanna ; Bari, M. Rizuanul ; Hoa, Nguyen K. ; Bianchi, Marta ; Rakonjac, Marija ; Rådmark, Olof ; Östenson, Claes-Göran ; Islam, Md. 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Shahidul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic β-cells</atitle><jtitle>Cell calcium (Edinburgh)</jtitle><addtitle>Cell Calcium</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>39</volume><issue>6</issue><spage>529</spage><epage>537</epage><pages>529-537</pages><issn>0143-4160</issn><issn>1532-1991</issn><eissn>1532-1991</eissn><abstract>Pancreatic β-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca
2+ from intracellular stores in β-cells but the identity of the channels involved in the Ca
2+ release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca
2+ concentration changes in pancreatic β-cells. Cytosolic free Ca
2+ concentration was measured in fura-2-loaded INS-1E cells and in primary β-cells from Wistar rats. The increase of cytosolic Ca
2+ concentration induced by arachidonic acid (150
μM) was due to both Ca
2+ release from intracellular stores and influx of Ca
2+ from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca
2+ increase. The Ca
2+ release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50
μM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca
2+ release by arachidonic acid. However, ryanodine (100
μM), a blocker of ryanodine receptors, abolished the effect of arachidonic acid on Ca
2+ release in both types of cells. These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in β-cells.</abstract><cop>Netherlands</cop><pub>Elsevier India Pvt Ltd</pub><pmid>16620964</pmid><doi>10.1016/j.ceca.2006.02.003</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0143-4160 |
| ispartof | Cell calcium (Edinburgh), 2006-06, Vol.39 (6), p.529-537 |
| issn | 0143-4160 1532-1991 1532-1991 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_576041 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Arachidonic acid Arachidonic Acid - pharmacology Calcium channels Calcium Channels, L-Type - metabolism Calcium Signaling - drug effects Calcium signalling Cells, Cultured Endoplasmic Reticulum - secretion Inositol 1,4,5-Trisphosphate Receptors - metabolism Insulin-Secreting Cells - drug effects Insulin-Secreting Cells - metabolism Male Nimodipine - pharmacology Nitric Oxide - metabolism Pancreatic β-cells Rats Rats, Wistar Ryanodine Receptor Calcium Release Channel - metabolism Ryanodine receptors |
| title | Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic β-cells |
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