Isocratic RP-HPLC method for rutin determination in solid oral dosage forms
A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C 18 reverse-phase analytical column, the following conditions were chosen as optimal: mobi...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2007-01, Vol.43 (2), p.718-721 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 721 |
|---|---|
| container_issue | 2 |
| container_start_page | 718 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 43 |
| creator | KUNTIE, Vesna PEJIE, Natasa IVKOVIE, Branka VUJIE, Zorica ILIE, Katarina MIEIE, Svetlana VUKOJEVIE, Vladana |
| description | A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C
18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol–water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate
=
1
mL
min
−1 and temperature
T
=
40.0
°C. Linearity was observed in the concentration range 8–120
μg
mL
−1 with a correlation coefficient of 0.99982 and the limit of detection (LOD)
=
2.6
μg
mL
−1, and limit of quantification (LOQ)
=
8.0
μg
mL
−1. Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present. |
| doi_str_mv | 10.1016/j.jpba.2006.07.019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_574290</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708506004651</els_id><sourcerecordid>68375134</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-c10ced1b9ef5a4aab0c2ee276cf3893dfafd2f09de4da6a91f762d7248439823</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhi0EotvCH-CAcoFbFn_FjiUuaFVoxUpUqAdulmOPwUsSL3YC4t_jaFP2BCdbo-cZj-dF6AXBW4KJeHPYHo6d2VKMxRbLLSbqEdqQVrKaCv7lMdpgyUgtcdtcoMucDxjjhij-FF0QoShmVGzQx9scbTJTsNXnu_rmbr-rBpi-RVf5mKo0T2GsHEyQhjAWKo5VKeTYB1fFZPrKxWy-wgIP-Rl64k2f4fl6XqH799f3u5t6_-nD7e7dvrZckqm2BFtwpFPgG8ON6bClAFQK61mrmPPGO-qxcsCdEUYRLwV1kvKWM9VSdoXqU9v8C45zp48pDCb91tEEvZa-lxvoRnKqcOHbf_LHFN1ZehAJU4wQ0RT19Ukt3I8Z8qSHkC30vRkhzlmLlsmGMF5AegJtijkn8H9fIVgvYemDXsLSS1gaS13CKtLLtfvcDeDOyppOAV6tgMnW9D6Z0YZ85tryP8YX7u2Jg7L1nwGSzjbAWLYcEthJuxj-N8cfhzG0cw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68375134</pqid></control><display><type>article</type><title>Isocratic RP-HPLC method for rutin determination in solid oral dosage forms</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>KUNTIE, Vesna ; PEJIE, Natasa ; IVKOVIE, Branka ; VUJIE, Zorica ; ILIE, Katarina ; MIEIE, Svetlana ; VUKOJEVIE, Vladana</creator><creatorcontrib>KUNTIE, Vesna ; PEJIE, Natasa ; IVKOVIE, Branka ; VUJIE, Zorica ; ILIE, Katarina ; MIEIE, Svetlana ; VUKOJEVIE, Vladana</creatorcontrib><description>A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C
18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol–water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate
=
1
mL
min
−1 and temperature
T
=
40.0
°C. Linearity was observed in the concentration range 8–120
μg
mL
−1 with a correlation coefficient of 0.99982 and the limit of detection (LOD)
=
2.6
μg
mL
−1, and limit of quantification (LOQ)
=
8.0
μg
mL
−1. Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2006.07.019</identifier><identifier>PMID: 16920326</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Capsules ; Chromatography, High Pressure Liquid - methods ; Drug Stability ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydrogen-Ion Concentration ; Isocratic RP-HPLC ; Linear Models ; Medical sciences ; Medicin och hälsovetenskap ; Methanol - chemistry ; Pharmaceutical dosage forms ; Pharmaceutical Preparations - chemistry ; Pharmacology. Drug treatments ; Phosphoric Acids - chemistry ; Reproducibility of Results ; Rutin ; Rutin - analysis ; Solvents - chemistry ; Spectrophotometry, Ultraviolet - methods ; Tablets ; Temperature ; Time Factors ; Water - chemistry</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2007-01, Vol.43 (2), p.718-721</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-c10ced1b9ef5a4aab0c2ee276cf3893dfafd2f09de4da6a91f762d7248439823</citedby><cites>FETCH-LOGICAL-c471t-c10ced1b9ef5a4aab0c2ee276cf3893dfafd2f09de4da6a91f762d7248439823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2006.07.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18429346$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16920326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:13931165$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>KUNTIE, Vesna</creatorcontrib><creatorcontrib>PEJIE, Natasa</creatorcontrib><creatorcontrib>IVKOVIE, Branka</creatorcontrib><creatorcontrib>VUJIE, Zorica</creatorcontrib><creatorcontrib>ILIE, Katarina</creatorcontrib><creatorcontrib>MIEIE, Svetlana</creatorcontrib><creatorcontrib>VUKOJEVIE, Vladana</creatorcontrib><title>Isocratic RP-HPLC method for rutin determination in solid oral dosage forms</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C
18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol–water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate
=
1
mL
min
−1 and temperature
T
=
40.0
°C. Linearity was observed in the concentration range 8–120
μg
mL
−1 with a correlation coefficient of 0.99982 and the limit of detection (LOD)
=
2.6
μg
mL
−1, and limit of quantification (LOQ)
=
8.0
μg
mL
−1. Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Capsules</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Drug Stability</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Isocratic RP-HPLC</subject><subject>Linear Models</subject><subject>Medical sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Methanol - chemistry</subject><subject>Pharmaceutical dosage forms</subject><subject>Pharmaceutical Preparations - chemistry</subject><subject>Pharmacology. Drug treatments</subject><subject>Phosphoric Acids - chemistry</subject><subject>Reproducibility of Results</subject><subject>Rutin</subject><subject>Rutin - analysis</subject><subject>Solvents - chemistry</subject><subject>Spectrophotometry, Ultraviolet - methods</subject><subject>Tablets</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Water - chemistry</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EotvCH-CAcoFbFn_FjiUuaFVoxUpUqAdulmOPwUsSL3YC4t_jaFP2BCdbo-cZj-dF6AXBW4KJeHPYHo6d2VKMxRbLLSbqEdqQVrKaCv7lMdpgyUgtcdtcoMucDxjjhij-FF0QoShmVGzQx9scbTJTsNXnu_rmbr-rBpi-RVf5mKo0T2GsHEyQhjAWKo5VKeTYB1fFZPrKxWy-wgIP-Rl64k2f4fl6XqH799f3u5t6_-nD7e7dvrZckqm2BFtwpFPgG8ON6bClAFQK61mrmPPGO-qxcsCdEUYRLwV1kvKWM9VSdoXqU9v8C45zp48pDCb91tEEvZa-lxvoRnKqcOHbf_LHFN1ZehAJU4wQ0RT19Ukt3I8Z8qSHkC30vRkhzlmLlsmGMF5AegJtijkn8H9fIVgvYemDXsLSS1gaS13CKtLLtfvcDeDOyppOAV6tgMnW9D6Z0YZ85tryP8YX7u2Jg7L1nwGSzjbAWLYcEthJuxj-N8cfhzG0cw</recordid><startdate>20070117</startdate><enddate>20070117</enddate><creator>KUNTIE, Vesna</creator><creator>PEJIE, Natasa</creator><creator>IVKOVIE, Branka</creator><creator>VUJIE, Zorica</creator><creator>ILIE, Katarina</creator><creator>MIEIE, Svetlana</creator><creator>VUKOJEVIE, Vladana</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20070117</creationdate><title>Isocratic RP-HPLC method for rutin determination in solid oral dosage forms</title><author>KUNTIE, Vesna ; PEJIE, Natasa ; IVKOVIE, Branka ; VUJIE, Zorica ; ILIE, Katarina ; MIEIE, Svetlana ; VUKOJEVIE, Vladana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-c10ced1b9ef5a4aab0c2ee276cf3893dfafd2f09de4da6a91f762d7248439823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Capsules</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Drug Stability</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Isocratic RP-HPLC</topic><topic>Linear Models</topic><topic>Medical sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Methanol - chemistry</topic><topic>Pharmaceutical dosage forms</topic><topic>Pharmaceutical Preparations - chemistry</topic><topic>Pharmacology. Drug treatments</topic><topic>Phosphoric Acids - chemistry</topic><topic>Reproducibility of Results</topic><topic>Rutin</topic><topic>Rutin - analysis</topic><topic>Solvents - chemistry</topic><topic>Spectrophotometry, Ultraviolet - methods</topic><topic>Tablets</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KUNTIE, Vesna</creatorcontrib><creatorcontrib>PEJIE, Natasa</creatorcontrib><creatorcontrib>IVKOVIE, Branka</creatorcontrib><creatorcontrib>VUJIE, Zorica</creatorcontrib><creatorcontrib>ILIE, Katarina</creatorcontrib><creatorcontrib>MIEIE, Svetlana</creatorcontrib><creatorcontrib>VUKOJEVIE, Vladana</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KUNTIE, Vesna</au><au>PEJIE, Natasa</au><au>IVKOVIE, Branka</au><au>VUJIE, Zorica</au><au>ILIE, Katarina</au><au>MIEIE, Svetlana</au><au>VUKOJEVIE, Vladana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isocratic RP-HPLC method for rutin determination in solid oral dosage forms</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2007-01-17</date><risdate>2007</risdate><volume>43</volume><issue>2</issue><spage>718</spage><epage>721</epage><pages>718-721</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C
18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol–water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate
=
1
mL
min
−1 and temperature
T
=
40.0
°C. Linearity was observed in the concentration range 8–120
μg
mL
−1 with a correlation coefficient of 0.99982 and the limit of detection (LOD)
=
2.6
μg
mL
−1, and limit of quantification (LOQ)
=
8.0
μg
mL
−1. Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16920326</pmid><doi>10.1016/j.jpba.2006.07.019</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2007-01, Vol.43 (2), p.718-721 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_574290 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Capsules Chromatography, High Pressure Liquid - methods Drug Stability Fundamental and applied biological sciences. Psychology General pharmacology Hydrogen-Ion Concentration Isocratic RP-HPLC Linear Models Medical sciences Medicin och hälsovetenskap Methanol - chemistry Pharmaceutical dosage forms Pharmaceutical Preparations - chemistry Pharmacology. Drug treatments Phosphoric Acids - chemistry Reproducibility of Results Rutin Rutin - analysis Solvents - chemistry Spectrophotometry, Ultraviolet - methods Tablets Temperature Time Factors Water - chemistry |
| title | Isocratic RP-HPLC method for rutin determination in solid oral dosage forms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T23%3A22%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isocratic%20RP-HPLC%20method%20for%20rutin%20determination%20in%20solid%20oral%20dosage%20forms&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=KUNTIE,%20Vesna&rft.date=2007-01-17&rft.volume=43&rft.issue=2&rft.spage=718&rft.epage=721&rft.pages=718-721&rft.issn=0731-7085&rft.eissn=1873-264X&rft.coden=JPBADA&rft_id=info:doi/10.1016/j.jpba.2006.07.019&rft_dat=%3Cproquest_swepu%3E68375134%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68375134&rft_id=info:pmid/16920326&rft_els_id=S0731708506004651&rfr_iscdi=true |