Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice
The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr⁺/- CETP⁺/-). After ovariectomy at 8 wk of age they were...
Gespeichert in:
| Veröffentlicht in: | Lipids 2006-07, Vol.41 (7), p.655-662 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 662 |
|---|---|
| container_issue | 7 |
| container_start_page | 655 |
| container_title | Lipids |
| container_volume | 41 |
| creator | Asakura, Leiko Cazita, Patrícia M Harada, Lila M Nunes, Valéria S Berti, Jairo A Salerno, Alessandro G Ketelhuth, Daniel F. J Gidlund, Magnus Oliveria, Helena C. F Quintão, Eder C. R |
| description | The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr⁺/- CETP⁺/-). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n=25), or at high-dose (Iso High, 0.535, n=28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n=28). Aortic root lipid-stained lesion area (mean μm²x10³±SD) did not differ among Iso Low (12.3±9.9), Iso High (7.4±6.4), and controls (10.7±12.8). Autoantibody titers against plasma oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human [4-¹⁴C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained area in the aortic root. |
| doi_str_mv | 10.1007/s11745-006-5016-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_573227</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1149253791</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4365-febacab1842e664802b9931d8bd0c6eecd0ed1067a0937eaa6e2e85a270589de3</originalsourceid><addsrcrecordid>eNqFks1u1DAUhSMEokPhAdiAxYJd4DrxT7JE09JWGolKbdeW49xMXRw7xEmreRceFkcZQGLDxj9X3zn29XGWvaXwiQLIz5FSyXgOIHIOVOTyWbahnFd5XYJ8nm0ACpazAuhJ9irGh7SlrOYvsxMqQdQlqzfZz5twIMMYJrSemOAnbb31e2Jj6Jx-DB4j6dI86sYdiPWdm9GbVOy1GcNwr_dInB3Cb4seJ90EZ2NPmnkiPkxkukfS4iO6MPToJxI6olNtDNG4ZbQx-ZLt-e01mUbt4x69NaS3Bl9nLzrtIr45zqfZ3dfz2-1lvvt2cbX9sssNKwXPO2y00Q2tWIFCsAqKpq5L2lZNC0YgmhawpSCkhrqUqLXAAiuuCwm8qlssT7N89Y1POMyNGkbb6_GggrbqWPqeVqi4LItCJv7jyqeuf8wYJ9XbaNA57THMUYkaoKxEkcAP_4APYR596kUVkgkmBV0gukLpQWMcsftzPgW15KzWnFXKWS05q-UG747Gc9Nj-1dxDDYBcgWerMPD_x3V7ur6DATnSfl-VXY6KL0fbVR3N-kPlUnHJKWi_AWH5MG9</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>274647612</pqid></control><display><type>article</type><title>Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>SpringerLink Journals</source><creator>Asakura, Leiko ; Cazita, Patrícia M ; Harada, Lila M ; Nunes, Valéria S ; Berti, Jairo A ; Salerno, Alessandro G ; Ketelhuth, Daniel F. J ; Gidlund, Magnus ; Oliveria, Helena C. F ; Quintão, Eder C. R</creator><creatorcontrib>Asakura, Leiko ; Cazita, Patrícia M ; Harada, Lila M ; Nunes, Valéria S ; Berti, Jairo A ; Salerno, Alessandro G ; Ketelhuth, Daniel F. J ; Gidlund, Magnus ; Oliveria, Helena C. F ; Quintão, Eder C. R</creatorcontrib><description>The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr⁺/- CETP⁺/-). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n=25), or at high-dose (Iso High, 0.535, n=28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n=28). Aortic root lipid-stained lesion area (mean μm²x10³±SD) did not differ among Iso Low (12.3±9.9), Iso High (7.4±6.4), and controls (10.7±12.8). Autoantibody titers against plasma oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human [4-¹⁴C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained area in the aortic root.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/s11745-006-5016-7</identifier><identifier>PMID: 17069349</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Animals ; apolipoproteins ; atherosclerosis ; Atherosclerosis - diet therapy ; Atherosclerosis - genetics ; Atherosclerosis - metabolism ; blood lipids ; blood proteins ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cholesterol ; Cholesterol Ester Transfer Proteins ; Diet ; dietary protein ; Disease Models, Animal ; Disease Progression ; Female ; Glycoproteins - genetics ; Glycoproteins - metabolism ; Heterozygote ; Humans ; isoflavones ; Isoflavones - pharmacology ; Lipids - blood ; lipoproteins ; Lipoproteins - blood ; Lipoproteins - metabolism ; low density lipoprotein ; macrophages ; Macrophages - chemistry ; Macrophages - drug effects ; Macrophages - metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Ovariectomy ; plant source protein ; protein metabolism ; Receptors, LDL - genetics ; soy protein ; Soybean Proteins - chemistry ; Soybean Proteins - pharmacology ; Transgenes ; transgenic animals ; transport proteins</subject><ispartof>Lipids, 2006-07, Vol.41 (7), p.655-662</ispartof><rights>2006 American Oil Chemists' Society (AOCS)</rights><rights>Copyright AOCS Press Jul 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4365-febacab1842e664802b9931d8bd0c6eecd0ed1067a0937eaa6e2e85a270589de3</citedby><cites>FETCH-LOGICAL-c4365-febacab1842e664802b9931d8bd0c6eecd0ed1067a0937eaa6e2e85a270589de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1007%2Fs11745-006-5016-7$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1007%2Fs11745-006-5016-7$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17069349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:112367296$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Asakura, Leiko</creatorcontrib><creatorcontrib>Cazita, Patrícia M</creatorcontrib><creatorcontrib>Harada, Lila M</creatorcontrib><creatorcontrib>Nunes, Valéria S</creatorcontrib><creatorcontrib>Berti, Jairo A</creatorcontrib><creatorcontrib>Salerno, Alessandro G</creatorcontrib><creatorcontrib>Ketelhuth, Daniel F. J</creatorcontrib><creatorcontrib>Gidlund, Magnus</creatorcontrib><creatorcontrib>Oliveria, Helena C. F</creatorcontrib><creatorcontrib>Quintão, Eder C. R</creatorcontrib><title>Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice</title><title>Lipids</title><addtitle>Lipids</addtitle><description>The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr⁺/- CETP⁺/-). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n=25), or at high-dose (Iso High, 0.535, n=28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n=28). Aortic root lipid-stained lesion area (mean μm²x10³±SD) did not differ among Iso Low (12.3±9.9), Iso High (7.4±6.4), and controls (10.7±12.8). Autoantibody titers against plasma oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human [4-¹⁴C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained area in the aortic root.</description><subject>Animals</subject><subject>apolipoproteins</subject><subject>atherosclerosis</subject><subject>Atherosclerosis - diet therapy</subject><subject>Atherosclerosis - genetics</subject><subject>Atherosclerosis - metabolism</subject><subject>blood lipids</subject><subject>blood proteins</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cholesterol</subject><subject>Cholesterol Ester Transfer Proteins</subject><subject>Diet</subject><subject>dietary protein</subject><subject>Disease Models, Animal</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - metabolism</subject><subject>Heterozygote</subject><subject>Humans</subject><subject>isoflavones</subject><subject>Isoflavones - pharmacology</subject><subject>Lipids - blood</subject><subject>lipoproteins</subject><subject>Lipoproteins - blood</subject><subject>Lipoproteins - metabolism</subject><subject>low density lipoprotein</subject><subject>macrophages</subject><subject>Macrophages - chemistry</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Ovariectomy</subject><subject>plant source protein</subject><subject>protein metabolism</subject><subject>Receptors, LDL - genetics</subject><subject>soy protein</subject><subject>Soybean Proteins - chemistry</subject><subject>Soybean Proteins - pharmacology</subject><subject>Transgenes</subject><subject>transgenic animals</subject><subject>transport proteins</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFks1u1DAUhSMEokPhAdiAxYJd4DrxT7JE09JWGolKbdeW49xMXRw7xEmreRceFkcZQGLDxj9X3zn29XGWvaXwiQLIz5FSyXgOIHIOVOTyWbahnFd5XYJ8nm0ACpazAuhJ9irGh7SlrOYvsxMqQdQlqzfZz5twIMMYJrSemOAnbb31e2Jj6Jx-DB4j6dI86sYdiPWdm9GbVOy1GcNwr_dInB3Cb4seJ90EZ2NPmnkiPkxkukfS4iO6MPToJxI6olNtDNG4ZbQx-ZLt-e01mUbt4x69NaS3Bl9nLzrtIr45zqfZ3dfz2-1lvvt2cbX9sssNKwXPO2y00Q2tWIFCsAqKpq5L2lZNC0YgmhawpSCkhrqUqLXAAiuuCwm8qlssT7N89Y1POMyNGkbb6_GggrbqWPqeVqi4LItCJv7jyqeuf8wYJ9XbaNA57THMUYkaoKxEkcAP_4APYR596kUVkgkmBV0gukLpQWMcsftzPgW15KzWnFXKWS05q-UG747Gc9Nj-1dxDDYBcgWerMPD_x3V7ur6DATnSfl-VXY6KL0fbVR3N-kPlUnHJKWi_AWH5MG9</recordid><startdate>200607</startdate><enddate>200607</enddate><creator>Asakura, Leiko</creator><creator>Cazita, Patrícia M</creator><creator>Harada, Lila M</creator><creator>Nunes, Valéria S</creator><creator>Berti, Jairo A</creator><creator>Salerno, Alessandro G</creator><creator>Ketelhuth, Daniel F. J</creator><creator>Gidlund, Magnus</creator><creator>Oliveria, Helena C. F</creator><creator>Quintão, Eder C. R</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer‐Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>200607</creationdate><title>Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice</title><author>Asakura, Leiko ; Cazita, Patrícia M ; Harada, Lila M ; Nunes, Valéria S ; Berti, Jairo A ; Salerno, Alessandro G ; Ketelhuth, Daniel F. J ; Gidlund, Magnus ; Oliveria, Helena C. F ; Quintão, Eder C. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4365-febacab1842e664802b9931d8bd0c6eecd0ed1067a0937eaa6e2e85a270589de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>apolipoproteins</topic><topic>atherosclerosis</topic><topic>Atherosclerosis - diet therapy</topic><topic>Atherosclerosis - genetics</topic><topic>Atherosclerosis - metabolism</topic><topic>blood lipids</topic><topic>blood proteins</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cholesterol</topic><topic>Cholesterol Ester Transfer Proteins</topic><topic>Diet</topic><topic>dietary protein</topic><topic>Disease Models, Animal</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - metabolism</topic><topic>Heterozygote</topic><topic>Humans</topic><topic>isoflavones</topic><topic>Isoflavones - pharmacology</topic><topic>Lipids - blood</topic><topic>lipoproteins</topic><topic>Lipoproteins - blood</topic><topic>Lipoproteins - metabolism</topic><topic>low density lipoprotein</topic><topic>macrophages</topic><topic>Macrophages - chemistry</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Ovariectomy</topic><topic>plant source protein</topic><topic>protein metabolism</topic><topic>Receptors, LDL - genetics</topic><topic>soy protein</topic><topic>Soybean Proteins - chemistry</topic><topic>Soybean Proteins - pharmacology</topic><topic>Transgenes</topic><topic>transgenic animals</topic><topic>transport proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asakura, Leiko</creatorcontrib><creatorcontrib>Cazita, Patrícia M</creatorcontrib><creatorcontrib>Harada, Lila M</creatorcontrib><creatorcontrib>Nunes, Valéria S</creatorcontrib><creatorcontrib>Berti, Jairo A</creatorcontrib><creatorcontrib>Salerno, Alessandro G</creatorcontrib><creatorcontrib>Ketelhuth, Daniel F. J</creatorcontrib><creatorcontrib>Gidlund, Magnus</creatorcontrib><creatorcontrib>Oliveria, Helena C. F</creatorcontrib><creatorcontrib>Quintão, Eder C. R</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asakura, Leiko</au><au>Cazita, Patrícia M</au><au>Harada, Lila M</au><au>Nunes, Valéria S</au><au>Berti, Jairo A</au><au>Salerno, Alessandro G</au><au>Ketelhuth, Daniel F. J</au><au>Gidlund, Magnus</au><au>Oliveria, Helena C. F</au><au>Quintão, Eder C. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice</atitle><jtitle>Lipids</jtitle><addtitle>Lipids</addtitle><date>2006-07</date><risdate>2006</risdate><volume>41</volume><issue>7</issue><spage>655</spage><epage>662</epage><pages>655-662</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><abstract>The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr⁺/- CETP⁺/-). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n=25), or at high-dose (Iso High, 0.535, n=28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n=28). Aortic root lipid-stained lesion area (mean μm²x10³±SD) did not differ among Iso Low (12.3±9.9), Iso High (7.4±6.4), and controls (10.7±12.8). Autoantibody titers against plasma oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human [4-¹⁴C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained area in the aortic root.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17069349</pmid><doi>10.1007/s11745-006-5016-7</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-4201 |
| ispartof | Lipids, 2006-07, Vol.41 (7), p.655-662 |
| issn | 0024-4201 1558-9307 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_573227 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; SpringerLink Journals |
| subjects | Animals apolipoproteins atherosclerosis Atherosclerosis - diet therapy Atherosclerosis - genetics Atherosclerosis - metabolism blood lipids blood proteins Carrier Proteins - genetics Carrier Proteins - metabolism Cholesterol Cholesterol Ester Transfer Proteins Diet dietary protein Disease Models, Animal Disease Progression Female Glycoproteins - genetics Glycoproteins - metabolism Heterozygote Humans isoflavones Isoflavones - pharmacology Lipids - blood lipoproteins Lipoproteins - blood Lipoproteins - metabolism low density lipoprotein macrophages Macrophages - chemistry Macrophages - drug effects Macrophages - metabolism Mice Mice, Inbred C57BL Mice, Transgenic Ovariectomy plant source protein protein metabolism Receptors, LDL - genetics soy protein Soybean Proteins - chemistry Soybean Proteins - pharmacology Transgenes transgenic animals transport proteins |
| title | Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T05%3A21%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Soy%20protein%20containing%20isoflavones%20favorably%20influences%20macrophage%20lipoprotein%20metabolism%20but%20not%20the%20development%20of%20atherosclerosis%20in%20CETP%20transgenic%20mice&rft.jtitle=Lipids&rft.au=Asakura,%20Leiko&rft.date=2006-07&rft.volume=41&rft.issue=7&rft.spage=655&rft.epage=662&rft.pages=655-662&rft.issn=0024-4201&rft.eissn=1558-9307&rft_id=info:doi/10.1007/s11745-006-5016-7&rft_dat=%3Cproquest_swepu%3E1149253791%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=274647612&rft_id=info:pmid/17069349&rfr_iscdi=true |