Gene expression analysis of human endometrial endothelial cells exposed to op′-DDT
The endocrine disrupting chemical o, p′-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 µM o,p′-DDT decreased their proliferation compared with the control. Microarray analyse...
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| Veröffentlicht in: | Molecular human reproduction 2008-02, Vol.14 (2), p.97-106 |
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| creator | Bredhult, C. Sahlin, L. Olovsson, M. |
| description | The endocrine disrupting chemical o, p′-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 µM o,p′-DDT decreased their proliferation compared with the control. Microarray analyses revealed that o,p′-DDT affected biological processes such as the cell cycle, cell division, defence response and lipid and steroid metabolism, in cellular components such as the plasma membrane and chromosomes, with molecular functions involved in signalling, receptor and cytokine activity, confirming the results of the proliferation assay. Expression of five of the most differentially expressed genes identified in the microarray analysis was verified by real-time quantitative reverse transcription polymerase chain reaction in five HEEC cultures obtained from women in the proliferative phase and in five cultures obtained from women in the secretory phase of the menstrual cycle after treatment with o,p′-DDT. The present study supports our previous findings of decreased proliferation and increased cell death in response to o,p′-DDT and may offer important clues to the mechanisms of action of o,p′-DDT. |
| doi_str_mv | 10.1093/molehr/gam091 |
| format | Article |
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Treatment of human endometrial endothelial cells (HEECs) with 50 µM o,p′-DDT decreased their proliferation compared with the control. Microarray analyses revealed that o,p′-DDT affected biological processes such as the cell cycle, cell division, defence response and lipid and steroid metabolism, in cellular components such as the plasma membrane and chromosomes, with molecular functions involved in signalling, receptor and cytokine activity, confirming the results of the proliferation assay. Expression of five of the most differentially expressed genes identified in the microarray analysis was verified by real-time quantitative reverse transcription polymerase chain reaction in five HEEC cultures obtained from women in the proliferative phase and in five cultures obtained from women in the secretory phase of the menstrual cycle after treatment with o,p′-DDT. The present study supports our previous findings of decreased proliferation and increased cell death in response to o,p′-DDT and may offer important clues to the mechanisms of action of o,p′-DDT.</description><identifier>ISSN: 1360-9947</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/gam091</identifier><identifier>PMID: 18204070</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Cells, Cultured ; DDT - chemistry ; DDT - pharmacology ; Embryology: invertebrates and vertebrates. Teratology ; endometrial cells ; Endometrium - cytology ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Humans ; in vitro cell cultures ; microarray ; Oligonucleotide Array Sequence Analysis ; p′-DDT ; reproductive tissue ; Reverse Transcriptase Polymerase Chain Reaction</subject><ispartof>Molecular human reproduction, 2008-02, Vol.14 (2), p.97-106</ispartof><rights>2008 INIST-CNRS</rights><rights>The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-a8b1a8e4614f883f2f49a4b4dd45ec7e31370678e727b0dfc5b85b4c618a3e603</citedby><cites>FETCH-LOGICAL-c494t-a8b1a8e4614f883f2f49a4b4dd45ec7e31370678e727b0dfc5b85b4c618a3e603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20197174$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18204070$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:117265973$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Bredhult, C.</creatorcontrib><creatorcontrib>Sahlin, L.</creatorcontrib><creatorcontrib>Olovsson, M.</creatorcontrib><title>Gene expression analysis of human endometrial endothelial cells exposed to op′-DDT</title><title>Molecular human reproduction</title><addtitle>Mol Hum Reprod</addtitle><description>The endocrine disrupting chemical o, p′-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 µM o,p′-DDT decreased their proliferation compared with the control. Microarray analyses revealed that o,p′-DDT affected biological processes such as the cell cycle, cell division, defence response and lipid and steroid metabolism, in cellular components such as the plasma membrane and chromosomes, with molecular functions involved in signalling, receptor and cytokine activity, confirming the results of the proliferation assay. Expression of five of the most differentially expressed genes identified in the microarray analysis was verified by real-time quantitative reverse transcription polymerase chain reaction in five HEEC cultures obtained from women in the proliferative phase and in five cultures obtained from women in the secretory phase of the menstrual cycle after treatment with o,p′-DDT. The present study supports our previous findings of decreased proliferation and increased cell death in response to o,p′-DDT and may offer important clues to the mechanisms of action of o,p′-DDT.</description><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>DDT - chemistry</subject><subject>DDT - pharmacology</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>endometrial cells</subject><subject>Endometrium - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>in vitro cell cultures</subject><subject>microarray</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>p′-DDT</subject><subject>reproductive tissue</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><issn>1360-9947</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EoqWwZIsiJNilteP_JZpCi1TBgkFC3VhOcsOkTeLgm4h2xzPxSDwJnk40I7HBGx_Zn-89voeQl4yeMmr5WR862MSz776nlj0ix0womheC6sdJ86StFfqIPEO8oZTpQpqn5IiZgiaEHpP1BQyQwd0YAbENQ-YH391ji1loss3c-yGDoQ49TLH13YOeNtBtdQVdh9unAaHOppCF8c-v3_n5-fo5edL4DuHFsp-Qrx_er1eX-dXni4-rd1d5JayYcm9K5g0IxURjDG-KRlgvSlHXQkKlgTOuqdIGdKFLWjeVLI0sRaWY8RwU5Sck39XFnzDOpRtj2_t474Jv3XJ0mxQ4qRSnIvFvd_wYw48ZcHJ9i9tv-AHCjE5TTg1P638gs5pKxmQCX_8D3oQ5phGiKwqZhiytPdisYkCM0OyNMuq2IbpdiG4XYuJfLUXnsof6QC-pJeDNAnisfNdEP1Qt7rmCJn9Mi0PjFie429_7eOuU5lq6y2_XTl1Lrj6tV-4L_wttE7bG</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Bredhult, C.</creator><creator>Sahlin, L.</creator><creator>Olovsson, M.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20080201</creationdate><title>Gene expression analysis of human endometrial endothelial cells exposed to op′-DDT</title><author>Bredhult, C. ; Sahlin, L. ; Olovsson, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-a8b1a8e4614f883f2f49a4b4dd45ec7e31370678e727b0dfc5b85b4c618a3e603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>DDT - chemistry</topic><topic>DDT - pharmacology</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>endometrial cells</topic><topic>Endometrium - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>in vitro cell cultures</topic><topic>microarray</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>p′-DDT</topic><topic>reproductive tissue</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bredhult, C.</creatorcontrib><creatorcontrib>Sahlin, L.</creatorcontrib><creatorcontrib>Olovsson, M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bredhult, C.</au><au>Sahlin, L.</au><au>Olovsson, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression analysis of human endometrial endothelial cells exposed to op′-DDT</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol Hum Reprod</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>14</volume><issue>2</issue><spage>97</spage><epage>106</epage><pages>97-106</pages><issn>1360-9947</issn><eissn>1460-2407</eissn><abstract>The endocrine disrupting chemical o, p′-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 µM o,p′-DDT decreased their proliferation compared with the control. Microarray analyses revealed that o,p′-DDT affected biological processes such as the cell cycle, cell division, defence response and lipid and steroid metabolism, in cellular components such as the plasma membrane and chromosomes, with molecular functions involved in signalling, receptor and cytokine activity, confirming the results of the proliferation assay. Expression of five of the most differentially expressed genes identified in the microarray analysis was verified by real-time quantitative reverse transcription polymerase chain reaction in five HEEC cultures obtained from women in the proliferative phase and in five cultures obtained from women in the secretory phase of the menstrual cycle after treatment with o,p′-DDT. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Biological and medical sciences Cells, Cultured DDT - chemistry DDT - pharmacology Embryology: invertebrates and vertebrates. Teratology endometrial cells Endometrium - cytology Endothelial Cells - drug effects Endothelial Cells - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Humans in vitro cell cultures microarray Oligonucleotide Array Sequence Analysis p′-DDT reproductive tissue Reverse Transcriptase Polymerase Chain Reaction |
| title | Gene expression analysis of human endometrial endothelial cells exposed to op′-DDT |
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