Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses
Acute respiratory tract infections are caused by a large number of viruses. Diagnostic methods have until recently been available only for a limited number of these viruses. With the objective to achieve sensitive assays for all respiratory viruses, a rational workflow in the laboratory, and a short...
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| Veröffentlicht in: | Journal of medical virology 2009-01, Vol.81 (1), p.167-175 |
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| container_title | Journal of medical virology |
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| creator | Tiveljung-Lindell, Annika Rotzén-Östlund, Maria Gupta, Shawon Ullstrand, Richard Grillner, Lena Zweygberg-Wirgart, Benita Allander, Tobias |
| description | Acute respiratory tract infections are caused by a large number of viruses. Diagnostic methods have until recently been available only for a limited number of these viruses. With the objective to achieve sensitive assays for all respiratory viruses, a rational workflow in the laboratory, and a short turn‐around time, a real‐time PCR diagnostic platform for daily rapid detection of 15 respiratory viruses was developed. The system was evaluated on 585 stored nasopharyngeal aspirates from hospitalized children. Previous analysis by immunofluorescence and virus isolation identified viruses in 37% of the samples while the new PCR diagnostic panel detected 57% virus positive samples. The new platform was introduced in the laboratory in October 2007 and has then fully replaced the standard immunofluorescence assay for rapid detection of viruses and virus isolation. J. Med. Virol. 81:167–175, 2009. © 2008 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jmv.21368 |
| format | Article |
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Diagnostic methods have until recently been available only for a limited number of these viruses. With the objective to achieve sensitive assays for all respiratory viruses, a rational workflow in the laboratory, and a short turn‐around time, a real‐time PCR diagnostic platform for daily rapid detection of 15 respiratory viruses was developed. The system was evaluated on 585 stored nasopharyngeal aspirates from hospitalized children. Previous analysis by immunofluorescence and virus isolation identified viruses in 37% of the samples while the new PCR diagnostic panel detected 57% virus positive samples. The new platform was introduced in the laboratory in October 2007 and has then fully replaced the standard immunofluorescence assay for rapid detection of viruses and virus isolation. J. Med. Virol. 81:167–175, 2009. © 2008 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.21368</identifier><identifier>PMID: 19031448</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adolescent ; Biological and medical sciences ; Child ; Child, Preschool ; Exudates and Transudates - virology ; Female ; Fundamental and applied biological sciences. Psychology ; human bocavirus ; Human viral diseases ; Humans ; Infant ; Infant, Newborn ; Infectious diseases ; influenza ; Male ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Diagnostic Techniques - methods ; Nasopharynx - virology ; Polymerase Chain Reaction - methods ; real-time PCR ; respiratory syncytial virus ; respiratory tract infection ; Respiratory Tract Infections - virology ; Sensitivity and Specificity ; Viral diseases ; Virology ; Virus Diseases - diagnosis ; Viruses - isolation & purification ; Young Adult</subject><ispartof>Journal of medical virology, 2009-01, Vol.81 (1), p.167-175</ispartof><rights>Copyright © 2008 Wiley‐Liss, Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6808-20012a52428c35cb8740a772546a67599394eb8f446998cb01ebb361517c025f3</citedby><cites>FETCH-LOGICAL-c6808-20012a52428c35cb8740a772546a67599394eb8f446998cb01ebb361517c025f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.21368$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.21368$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,550,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20939680$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19031448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:117910190$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Tiveljung-Lindell, Annika</creatorcontrib><creatorcontrib>Rotzén-Östlund, Maria</creatorcontrib><creatorcontrib>Gupta, Shawon</creatorcontrib><creatorcontrib>Ullstrand, Richard</creatorcontrib><creatorcontrib>Grillner, Lena</creatorcontrib><creatorcontrib>Zweygberg-Wirgart, Benita</creatorcontrib><creatorcontrib>Allander, Tobias</creatorcontrib><title>Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Acute respiratory tract infections are caused by a large number of viruses. Diagnostic methods have until recently been available only for a limited number of these viruses. With the objective to achieve sensitive assays for all respiratory viruses, a rational workflow in the laboratory, and a short turn‐around time, a real‐time PCR diagnostic platform for daily rapid detection of 15 respiratory viruses was developed. The system was evaluated on 585 stored nasopharyngeal aspirates from hospitalized children. Previous analysis by immunofluorescence and virus isolation identified viruses in 37% of the samples while the new PCR diagnostic panel detected 57% virus positive samples. The new platform was introduced in the laboratory in October 2007 and has then fully replaced the standard immunofluorescence assay for rapid detection of viruses and virus isolation. J. Med. Virol. 81:167–175, 2009. © 2008 Wiley‐Liss, Inc.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Exudates and Transudates - virology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>human bocavirus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infectious diseases</subject><subject>influenza</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Nasopharynx - virology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>real-time PCR</subject><subject>respiratory syncytial virus</subject><subject>respiratory tract infection</subject><subject>Respiratory Tract Infections - virology</subject><subject>Sensitivity and Specificity</subject><subject>Viral diseases</subject><subject>Virology</subject><subject>Virus Diseases - diagnosis</subject><subject>Viruses - isolation & purification</subject><subject>Young Adult</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNqNkk1v1DAQhiMEokvhwB9AvoDgkHbsxB-5IKECLWW7XAocLcfrFLdOHOxky_57vN10oQc-Lv585h3NzJtlTzEcYAByeNmuDggumLiXzTBULK-A4_vZDHDJcsYw3csexXgJAKIi5GG2hysocFmKWTa-NSvjfN-abkCqWyLb9s5sbmqwvkO-QQq13hk9OhXQ0qqLzsfBatQ7NTQ-tCgtaKmsW6OgertESzMYfRuMKQom9jaowYc1WtkwRhMfZw8a5aJ5Mu372ef3786PTvL5p-MPR2_muWYCRE4AMFGUlETogupa8BIU54SWTDFOq6qoSlOLpixZVQldAzZ1XaR6MddAaFPsZ_lWN16bfqxlH2yrwlp6ZeX0dJVORlKGQYjEv97y6ac1S53aEJS7E3b3p7Pf5IVfSY4Zx5QmgReTQPDfRxMH2dqojXOqM36MkjFOyoqRf4IECkHTFP8DBCq42Ci-_CuIKQNcABeQ0FdbVAcfYzDNrkIMcuMomRwlbxyV2Ge_t-QXOVkoAc8nQEWtXBNUp23ccQTSlNhN0sMtd22dWf85ozw9-3KbepqejYP5sYtQ4UoyXnAqvy6O5elifn72ccHlSfET3EryvQ</recordid><startdate>200901</startdate><enddate>200901</enddate><creator>Tiveljung-Lindell, Annika</creator><creator>Rotzén-Östlund, Maria</creator><creator>Gupta, Shawon</creator><creator>Ullstrand, Richard</creator><creator>Grillner, Lena</creator><creator>Zweygberg-Wirgart, Benita</creator><creator>Allander, Tobias</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>200901</creationdate><title>Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses</title><author>Tiveljung-Lindell, Annika ; Rotzén-Östlund, Maria ; Gupta, Shawon ; Ullstrand, Richard ; Grillner, Lena ; Zweygberg-Wirgart, Benita ; Allander, Tobias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6808-20012a52428c35cb8740a772546a67599394eb8f446998cb01ebb361517c025f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adolescent</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Exudates and Transudates - virology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>human bocavirus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infectious diseases</topic><topic>influenza</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Nasopharynx - virology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>real-time PCR</topic><topic>respiratory syncytial virus</topic><topic>respiratory tract infection</topic><topic>Respiratory Tract Infections - virology</topic><topic>Sensitivity and Specificity</topic><topic>Viral diseases</topic><topic>Virology</topic><topic>Virus Diseases - diagnosis</topic><topic>Viruses - isolation & purification</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tiveljung-Lindell, Annika</creatorcontrib><creatorcontrib>Rotzén-Östlund, Maria</creatorcontrib><creatorcontrib>Gupta, Shawon</creatorcontrib><creatorcontrib>Ullstrand, Richard</creatorcontrib><creatorcontrib>Grillner, Lena</creatorcontrib><creatorcontrib>Zweygberg-Wirgart, Benita</creatorcontrib><creatorcontrib>Allander, Tobias</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tiveljung-Lindell, Annika</au><au>Rotzén-Östlund, Maria</au><au>Gupta, Shawon</au><au>Ullstrand, Richard</au><au>Grillner, Lena</au><au>Zweygberg-Wirgart, Benita</au><au>Allander, Tobias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; SWEPUB Freely available online |
| subjects | Adolescent Biological and medical sciences Child Child, Preschool Exudates and Transudates - virology Female Fundamental and applied biological sciences. Psychology human bocavirus Human viral diseases Humans Infant Infant, Newborn Infectious diseases influenza Male Medical sciences Microbiology Miscellaneous Molecular Diagnostic Techniques - methods Nasopharynx - virology Polymerase Chain Reaction - methods real-time PCR respiratory syncytial virus respiratory tract infection Respiratory Tract Infections - virology Sensitivity and Specificity Viral diseases Virology Virus Diseases - diagnosis Viruses - isolation & purification Young Adult |
| title | Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses |
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