Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli
Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acyl-CoA reductases (FARs) catalyze the formation of a fatty alcohol from an acyl-CoA. The Arabidopsis ( Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba ( Simmondsia ch...
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| Veröffentlicht in: | Journal of plant physiology 2009-01, Vol.166 (8), p.787-796 |
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| creator | Doan, Thuy T.P. Carlsson, Anders S. Hamberg, Mats Bülow, Leif Stymne, Sten Olsson, Peter |
| description | Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acyl-CoA reductases (FARs) catalyze the formation of a fatty alcohol from an acyl-CoA. The
Arabidopsis (
Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba (
Simmondsia chinensis), silk moth, wheat and mouse. Expression of six
Arabidopsis FAR homologs in
Escherichia coli resulted in production of alcohols from endogenous
E. coli fatty acids by five of these genes, confirming that they encode for FAR enzymes. Only a truncated splicing version of the sixth gene was found, and this gene yielded a protein with no FAR activity. The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in
E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells. |
| doi_str_mv | 10.1016/j.jplph.2008.10.003 |
| format | Article |
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Arabidopsis (
Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba (
Simmondsia chinensis), silk moth, wheat and mouse. Expression of six
Arabidopsis FAR homologs in
Escherichia coli resulted in production of alcohols from endogenous
E. coli fatty acids by five of these genes, confirming that they encode for FAR enzymes. Only a truncated splicing version of the sixth gene was found, and this gene yielded a protein with no FAR activity. The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in
E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells.</description><identifier>ISSN: 0176-1617</identifier><identifier>ISSN: 1618-1328</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1016/j.jplph.2008.10.003</identifier><identifier>PMID: 19062129</identifier><identifier>CODEN: JPPHEY</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Agricultural Science ; aldehyde oxidoreductases ; Aldehyde Oxidoreductases - chemistry ; Aldehyde Oxidoreductases - genetics ; Aldehyde Oxidoreductases - metabolism ; Amino Acid Sequence ; amino acid sequences ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis thaliana ; Biochemistry and Molecular Biology ; Biokemi och molekylärbiologi ; Biologi ; Biological and medical sciences ; Biological Sciences ; Chromatography, Thin Layer ; Cloning, Molecular ; complementary DNA ; Electrophoresis, Polyacrylamide Gel ; epicuticular wax ; Escherichia coli ; Escherichia coli - genetics ; fatty acid-CoA reductase ; Fatty acyl-CoA reductase ; Fatty alcohol ; fatty alcohols ; Fatty Alcohols - metabolism ; Fundamental and applied biological sciences. Psychology ; Förnyelsebar bioenergi ; Gene Expression ; Jojoba ; Jordbruksvetenskap ; molecular cloning ; Molecular Sequence Data ; NAD(P)H ; Natural Sciences ; Naturvetenskap ; plant cuticle ; Plant physiology and development ; protein synthesis ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Renewable Bioenergy Research ; Sequence Alignment ; sequence analysis ; Sequence Analysis, Protein ; Simmondsia chinensis ; Triticum aestivum ; Wax ester ; wax esters</subject><ispartof>Journal of plant physiology, 2009-01, Vol.166 (8), p.787-796</ispartof><rights>2008 Elsevier GmbH</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c653t-14222d16717b3eeae8a847f50ba9d50b8088881e863afdcf08e5b86ab7d7f1e83</citedby><cites>FETCH-LOGICAL-c653t-14222d16717b3eeae8a847f50ba9d50b8088881e863afdcf08e5b86ab7d7f1e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0176161708003180$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21416668$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19062129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://lup.lub.lu.se/record/1425621$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/61243$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:118794281$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Doan, Thuy T.P.</creatorcontrib><creatorcontrib>Carlsson, Anders S.</creatorcontrib><creatorcontrib>Hamberg, Mats</creatorcontrib><creatorcontrib>Bülow, Leif</creatorcontrib><creatorcontrib>Stymne, Sten</creatorcontrib><creatorcontrib>Olsson, Peter</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli</title><title>Journal of plant physiology</title><addtitle>J Plant Physiol</addtitle><description>Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acyl-CoA reductases (FARs) catalyze the formation of a fatty alcohol from an acyl-CoA. The
Arabidopsis (
Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba (
Simmondsia chinensis), silk moth, wheat and mouse. Expression of six
Arabidopsis FAR homologs in
Escherichia coli resulted in production of alcohols from endogenous
E. coli fatty acids by five of these genes, confirming that they encode for FAR enzymes. Only a truncated splicing version of the sixth gene was found, and this gene yielded a protein with no FAR activity. The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in
E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells.</description><subject>Agricultural Science</subject><subject>aldehyde oxidoreductases</subject><subject>Aldehyde Oxidoreductases - chemistry</subject><subject>Aldehyde Oxidoreductases - genetics</subject><subject>Aldehyde Oxidoreductases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Biochemistry and Molecular Biology</subject><subject>Biokemi och molekylärbiologi</subject><subject>Biologi</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences</subject><subject>Chromatography, Thin Layer</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>epicuticular wax</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>fatty acid-CoA reductase</subject><subject>Fatty acyl-CoA reductase</subject><subject>Fatty alcohol</subject><subject>fatty alcohols</subject><subject>Fatty Alcohols - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Förnyelsebar bioenergi</subject><subject>Gene Expression</subject><subject>Jojoba</subject><subject>Jordbruksvetenskap</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>NAD(P)H</subject><subject>Natural Sciences</subject><subject>Naturvetenskap</subject><subject>plant cuticle</subject><subject>Plant physiology and development</subject><subject>protein synthesis</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Renewable Bioenergy Research</subject><subject>Sequence Alignment</subject><subject>sequence analysis</subject><subject>Sequence Analysis, Protein</subject><subject>Simmondsia chinensis</subject><subject>Triticum aestivum</subject><subject>Wax ester</subject><subject>wax esters</subject><issn>0176-1617</issn><issn>1618-1328</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkuP0zAUhSMEYsrAL0ACb2CX4kf8yIJFVc0AUiUWMDsky3Gupy5pHOxkoP8eh1TDisGSX0ffPbq2TlG8JHhNMBHvDuvD0A37NcVYZWWNMXtUrIggqiSMqsfFChMpyizIi-JZSgec71yxp8UFqbGghNar4tv11NvRh950CH4NEVLKFxQccv4O0CaaxrdhSD4hZ8bxhIw9deU2bFCEdrKjSYBuoYeEfI-ukt1D9HbvDbKh88-LJ850CV6c98vi5vrq6_Zjufv84dN2syut4GwsSUUpbYmQRDYMwIAyqpKO48bUbV4VVnkQUIIZ11qHFfBGCdPIVross8uiXHzTTximRg_RH0086WC8Pkvf8wk0FzibPMx3U2PivM0FgtBq5nf_5LtpyLPRC884xXXFrTaO1TqXEl0LK7WSTEklGoYJz3ZvF7shhh8TpFEffbLQdaaHMCWdP0LUlKv_ghRLjmVVZZAtoI0hpQjuvkWC9RwWfdB_wqLnsMxiDkuuenW2n5ojtH9rzunIwJszYJI1nYumtz7dc5RURAgx9_l64ZwJ2tzGzNx8oZjk1woqMZeZeL8QkGNw5yF_sPXQW2h9BDvqNvgHW_0NXwrnag</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Doan, Thuy T.P.</creator><creator>Carlsson, Anders S.</creator><creator>Hamberg, Mats</creator><creator>Bülow, Leif</creator><creator>Stymne, Sten</creator><creator>Olsson, Peter</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D95</scope></search><sort><creationdate>20090101</creationdate><title>Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli</title><author>Doan, Thuy T.P. ; Carlsson, Anders S. ; Hamberg, Mats ; Bülow, Leif ; Stymne, Sten ; Olsson, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c653t-14222d16717b3eeae8a847f50ba9d50b8088881e863afdcf08e5b86ab7d7f1e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agricultural Science</topic><topic>aldehyde oxidoreductases</topic><topic>Aldehyde Oxidoreductases - chemistry</topic><topic>Aldehyde Oxidoreductases - genetics</topic><topic>Aldehyde Oxidoreductases - metabolism</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis thaliana</topic><topic>Biochemistry and Molecular Biology</topic><topic>Biokemi och molekylärbiologi</topic><topic>Biologi</topic><topic>Biological and medical sciences</topic><topic>Biological Sciences</topic><topic>Chromatography, Thin Layer</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>epicuticular wax</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>fatty acid-CoA reductase</topic><topic>Fatty acyl-CoA reductase</topic><topic>Fatty alcohol</topic><topic>fatty alcohols</topic><topic>Fatty Alcohols - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Förnyelsebar bioenergi</topic><topic>Gene Expression</topic><topic>Jojoba</topic><topic>Jordbruksvetenskap</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>NAD(P)H</topic><topic>Natural Sciences</topic><topic>Naturvetenskap</topic><topic>plant cuticle</topic><topic>Plant physiology and development</topic><topic>protein synthesis</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Renewable Bioenergy Research</topic><topic>Sequence Alignment</topic><topic>sequence analysis</topic><topic>Sequence Analysis, Protein</topic><topic>Simmondsia chinensis</topic><topic>Triticum aestivum</topic><topic>Wax ester</topic><topic>wax esters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doan, Thuy T.P.</creatorcontrib><creatorcontrib>Carlsson, Anders S.</creatorcontrib><creatorcontrib>Hamberg, Mats</creatorcontrib><creatorcontrib>Bülow, Leif</creatorcontrib><creatorcontrib>Stymne, Sten</creatorcontrib><creatorcontrib>Olsson, Peter</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Lunds universitet</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doan, Thuy T.P.</au><au>Carlsson, Anders S.</au><au>Hamberg, Mats</au><au>Bülow, Leif</au><au>Stymne, Sten</au><au>Olsson, Peter</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli</atitle><jtitle>Journal of plant physiology</jtitle><addtitle>J Plant Physiol</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>166</volume><issue>8</issue><spage>787</spage><epage>796</epage><pages>787-796</pages><issn>0176-1617</issn><issn>1618-1328</issn><eissn>1618-1328</eissn><coden>JPPHEY</coden><abstract>Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acyl-CoA reductases (FARs) catalyze the formation of a fatty alcohol from an acyl-CoA. The
Arabidopsis (
Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba (
Simmondsia chinensis), silk moth, wheat and mouse. Expression of six
Arabidopsis FAR homologs in
Escherichia coli resulted in production of alcohols from endogenous
E. coli fatty acids by five of these genes, confirming that they encode for FAR enzymes. Only a truncated splicing version of the sixth gene was found, and this gene yielded a protein with no FAR activity. The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in
E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>19062129</pmid><doi>10.1016/j.jplph.2008.10.003</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0176-1617 |
| ispartof | Journal of plant physiology, 2009-01, Vol.166 (8), p.787-796 |
| issn | 0176-1617 1618-1328 1618-1328 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_560863 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agricultural Science aldehyde oxidoreductases Aldehyde Oxidoreductases - chemistry Aldehyde Oxidoreductases - genetics Aldehyde Oxidoreductases - metabolism Amino Acid Sequence amino acid sequences Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis thaliana Biochemistry and Molecular Biology Biokemi och molekylärbiologi Biologi Biological and medical sciences Biological Sciences Chromatography, Thin Layer Cloning, Molecular complementary DNA Electrophoresis, Polyacrylamide Gel epicuticular wax Escherichia coli Escherichia coli - genetics fatty acid-CoA reductase Fatty acyl-CoA reductase Fatty alcohol fatty alcohols Fatty Alcohols - metabolism Fundamental and applied biological sciences. Psychology Förnyelsebar bioenergi Gene Expression Jojoba Jordbruksvetenskap molecular cloning Molecular Sequence Data NAD(P)H Natural Sciences Naturvetenskap plant cuticle Plant physiology and development protein synthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Renewable Bioenergy Research Sequence Alignment sequence analysis Sequence Analysis, Protein Simmondsia chinensis Triticum aestivum Wax ester wax esters |
| title | Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli |
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