Glutathione and amino acid concentrations in human liver during short warm ischaemia and reperfusion: a pilot study
Glutathione is a major antioxidant, and, in the present study, we investigated whether a clinical model of short warm ischaemia and reperfusion of the human liver during surgery would influence glutathione and amino acid metabolism. Previous studies in humans have demonstrated that ischaemia and rep...
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| Veröffentlicht in: | Clinical science (1979) 2009-11, Vol.117 (9-10), p.339-344 |
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| container_title | Clinical science (1979) |
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| creator | WESTMAN, Bo THÖRNE, Anders ROOYACKERS, Olav FREDRIKSSON, Katarina WERNERMAN, Jan HAMMARQVIST, Folke |
| description | Glutathione is a major antioxidant, and, in the present study, we investigated whether a clinical model of short warm ischaemia and reperfusion of the human liver during surgery would influence glutathione and amino acid metabolism. Previous studies in humans have demonstrated that ischaemia and reperfusion in skeletal muscle for up to 120 min have no major effect on muscle glutathione concentrations. Liver ischaemia and reperfusion in animals have demonstrated diverging results concerning glutathione metabolism. In the present study, six patients with liver malignancies, undergoing liver resection during warm ischaemia, were included. Liver biopsies were obtained from healthy appearing liver tissue from both lobes before ischaemia and at maximal ischaemia, and from the remaining liver lobe after 5, 10, 15, 20, 25 and 30 min of reperfusion. The biopsies were analysed for glutathione, amino acids and lactate. Median ischaemia time was 28 (range, 15-36) min. Lactate increased 266% at maximal ischaemia (P |
| doi_str_mv | 10.1042/CS20080654 |
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Previous studies in humans have demonstrated that ischaemia and reperfusion in skeletal muscle for up to 120 min have no major effect on muscle glutathione concentrations. Liver ischaemia and reperfusion in animals have demonstrated diverging results concerning glutathione metabolism. In the present study, six patients with liver malignancies, undergoing liver resection during warm ischaemia, were included. Liver biopsies were obtained from healthy appearing liver tissue from both lobes before ischaemia and at maximal ischaemia, and from the remaining liver lobe after 5, 10, 15, 20, 25 and 30 min of reperfusion. The biopsies were analysed for glutathione, amino acids and lactate. Median ischaemia time was 28 (range, 15-36) min. Lactate increased 266% at maximal ischaemia (P<0.05). No alterations in glutathione concentrations or the redox status of glutathione (GSH/total glutathione) were observed. Glutamate decreased 22% (P<0.05) at maximal ischaemia and increased thereafter 72% at 30 min of reperfusion (P<0.05). Alanine increased 105% at maximal ischaemia (P<0.05) and was normalized during reperfusion. BCAAs (branched-chain amino acids) increased 67% at maximal ischaemia (P<0.05). In conclusion, short-time ischaemia and reperfusion in the human liver did not affect glutathione concentrations, whereas changes were observed in amino acid concentrations during both ischaemia and reperfusion.</description><identifier>ISSN: 0143-5221</identifier><identifier>EISSN: 1470-8736</identifier><identifier>DOI: 10.1042/CS20080654</identifier><identifier>PMID: 19331647</identifier><identifier>CODEN: CSCIAE</identifier><language>eng</language><publisher>London: Portland Press</publisher><subject>Adult ; Aged ; Amino Acids - metabolism ; Biological and medical sciences ; Cardiology. Vascular system ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; General aspects ; Glutathione - metabolism ; Hepatectomy ; Humans ; Intraoperative Care - methods ; Lactic Acid - metabolism ; Liver - metabolism ; Liver Neoplasms - secondary ; Liver Neoplasms - surgery ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Middle Aged ; Other diseases. Semiology ; Oxidation-Reduction ; Oxidative Stress ; Pilot Projects ; Reperfusion Injury - metabolism ; Time Factors ; Warm Ischemia</subject><ispartof>Clinical science (1979), 2009-11, Vol.117 (9-10), p.339-344</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-d190b0be8be7b8eaad2acfeb83573a4037be5971d39f1d6d3f6aea000b84aea93</citedby><cites>FETCH-LOGICAL-c389t-d190b0be8be7b8eaad2acfeb83573a4037be5971d39f1d6d3f6aea000b84aea93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,550,776,881</link.rule.ids><linktorsrc>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:119541115$$EView_record_in_Swedish_Publication_Index_(SWEPUB)$$FView_record_in_$$GSwedish_Publication_Index_(SWEPUB)$$Hfree_for_read</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22036869$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19331647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:119541115$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>WESTMAN, Bo</creatorcontrib><creatorcontrib>THÖRNE, Anders</creatorcontrib><creatorcontrib>ROOYACKERS, Olav</creatorcontrib><creatorcontrib>FREDRIKSSON, Katarina</creatorcontrib><creatorcontrib>WERNERMAN, Jan</creatorcontrib><creatorcontrib>HAMMARQVIST, Folke</creatorcontrib><title>Glutathione and amino acid concentrations in human liver during short warm ischaemia and reperfusion: a pilot study</title><title>Clinical science (1979)</title><addtitle>Clin Sci (Lond)</addtitle><description>Glutathione is a major antioxidant, and, in the present study, we investigated whether a clinical model of short warm ischaemia and reperfusion of the human liver during surgery would influence glutathione and amino acid metabolism. Previous studies in humans have demonstrated that ischaemia and reperfusion in skeletal muscle for up to 120 min have no major effect on muscle glutathione concentrations. Liver ischaemia and reperfusion in animals have demonstrated diverging results concerning glutathione metabolism. In the present study, six patients with liver malignancies, undergoing liver resection during warm ischaemia, were included. Liver biopsies were obtained from healthy appearing liver tissue from both lobes before ischaemia and at maximal ischaemia, and from the remaining liver lobe after 5, 10, 15, 20, 25 and 30 min of reperfusion. The biopsies were analysed for glutathione, amino acids and lactate. Median ischaemia time was 28 (range, 15-36) min. Lactate increased 266% at maximal ischaemia (P<0.05). No alterations in glutathione concentrations or the redox status of glutathione (GSH/total glutathione) were observed. Glutamate decreased 22% (P<0.05) at maximal ischaemia and increased thereafter 72% at 30 min of reperfusion (P<0.05). Alanine increased 105% at maximal ischaemia (P<0.05) and was normalized during reperfusion. BCAAs (branched-chain amino acids) increased 67% at maximal ischaemia (P<0.05). In conclusion, short-time ischaemia and reperfusion in the human liver did not affect glutathione concentrations, whereas changes were observed in amino acid concentrations during both ischaemia and reperfusion.</description><subject>Adult</subject><subject>Aged</subject><subject>Amino Acids - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cardiology. Vascular system</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>General aspects</subject><subject>Glutathione - metabolism</subject><subject>Hepatectomy</subject><subject>Humans</subject><subject>Intraoperative Care - methods</subject><subject>Lactic Acid - metabolism</subject><subject>Liver - metabolism</subject><subject>Liver Neoplasms - secondary</subject><subject>Liver Neoplasms - surgery</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Other diseases. Semiology</subject><subject>Oxidation-Reduction</subject><subject>Oxidative Stress</subject><subject>Pilot Projects</subject><subject>Reperfusion Injury - metabolism</subject><subject>Time Factors</subject><subject>Warm Ischemia</subject><issn>0143-5221</issn><issn>1470-8736</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNpFkcGO1DAMhiMEYoeFCw-AcoEDUsFp2qTlhkawIK3EAThXTuMygTYpScNq357AVLv2wZb9-Zesn7HnAt4IaOq3x681QAeqbR6wg2g0VJ2W6iE7gGhk1da1uGBPUvoJUMuSj9mF6KUUqtEHlq7mvOF2csETR285Ls4HjqOzfAx-JL9F3Mo2cef5KS_o-ez-UOQ2R-d_8HQKceM3GBfu0nhCWhz-F4q0UpxyKrfvOPLVzWHjacv29il7NOGc6NleL9n3jx--HT9V11-uPh_fX1ej7PqtsqIHA4Y6Q9p0hGhrHCcynWy1xAakNtT2WljZT8IqKyeFhABguqY0vbxk1Vk33dCazbBGt2C8HQK6YR_9Kh0NbduVKPyrM7_G8DtT2oalvETzjJ5CToPSSvYAuoCvz-AYQ0qRpjtpAcM_S4Z7Swr8YlfNZiF7j-4eFODlDmAacZ4i-tGlO66uQapO9fIvOJCWWg</recordid><startdate>20091101</startdate><enddate>20091101</enddate><creator>WESTMAN, Bo</creator><creator>THÖRNE, Anders</creator><creator>ROOYACKERS, Olav</creator><creator>FREDRIKSSON, Katarina</creator><creator>WERNERMAN, Jan</creator><creator>HAMMARQVIST, Folke</creator><general>Portland Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20091101</creationdate><title>Glutathione and amino acid concentrations in human liver during short warm ischaemia and reperfusion: a pilot study</title><author>WESTMAN, Bo ; THÖRNE, Anders ; ROOYACKERS, Olav ; FREDRIKSSON, Katarina ; WERNERMAN, Jan ; HAMMARQVIST, Folke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-d190b0be8be7b8eaad2acfeb83573a4037be5971d39f1d6d3f6aea000b84aea93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Amino Acids - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cardiology. Vascular system</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>General aspects</topic><topic>Glutathione - metabolism</topic><topic>Hepatectomy</topic><topic>Humans</topic><topic>Intraoperative Care - methods</topic><topic>Lactic Acid - metabolism</topic><topic>Liver - metabolism</topic><topic>Liver Neoplasms - secondary</topic><topic>Liver Neoplasms - surgery</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Other diseases. Semiology</topic><topic>Oxidation-Reduction</topic><topic>Oxidative Stress</topic><topic>Pilot Projects</topic><topic>Reperfusion Injury - metabolism</topic><topic>Time Factors</topic><topic>Warm Ischemia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WESTMAN, Bo</creatorcontrib><creatorcontrib>THÖRNE, Anders</creatorcontrib><creatorcontrib>ROOYACKERS, Olav</creatorcontrib><creatorcontrib>FREDRIKSSON, Katarina</creatorcontrib><creatorcontrib>WERNERMAN, Jan</creatorcontrib><creatorcontrib>HAMMARQVIST, Folke</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Clinical science (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WESTMAN, Bo</au><au>THÖRNE, Anders</au><au>ROOYACKERS, Olav</au><au>FREDRIKSSON, Katarina</au><au>WERNERMAN, Jan</au><au>HAMMARQVIST, Folke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutathione and amino acid concentrations in human liver during short warm ischaemia and reperfusion: a pilot study</atitle><jtitle>Clinical science (1979)</jtitle><addtitle>Clin Sci (Lond)</addtitle><date>2009-11-01</date><risdate>2009</risdate><volume>117</volume><issue>9-10</issue><spage>339</spage><epage>344</epage><pages>339-344</pages><issn>0143-5221</issn><eissn>1470-8736</eissn><coden>CSCIAE</coden><abstract>Glutathione is a major antioxidant, and, in the present study, we investigated whether a clinical model of short warm ischaemia and reperfusion of the human liver during surgery would influence glutathione and amino acid metabolism. Previous studies in humans have demonstrated that ischaemia and reperfusion in skeletal muscle for up to 120 min have no major effect on muscle glutathione concentrations. Liver ischaemia and reperfusion in animals have demonstrated diverging results concerning glutathione metabolism. In the present study, six patients with liver malignancies, undergoing liver resection during warm ischaemia, were included. Liver biopsies were obtained from healthy appearing liver tissue from both lobes before ischaemia and at maximal ischaemia, and from the remaining liver lobe after 5, 10, 15, 20, 25 and 30 min of reperfusion. The biopsies were analysed for glutathione, amino acids and lactate. Median ischaemia time was 28 (range, 15-36) min. Lactate increased 266% at maximal ischaemia (P<0.05). No alterations in glutathione concentrations or the redox status of glutathione (GSH/total glutathione) were observed. Glutamate decreased 22% (P<0.05) at maximal ischaemia and increased thereafter 72% at 30 min of reperfusion (P<0.05). Alanine increased 105% at maximal ischaemia (P<0.05) and was normalized during reperfusion. BCAAs (branched-chain amino acids) increased 67% at maximal ischaemia (P<0.05). In conclusion, short-time ischaemia and reperfusion in the human liver did not affect glutathione concentrations, whereas changes were observed in amino acid concentrations during both ischaemia and reperfusion.</abstract><cop>London</cop><pub>Portland Press</pub><pmid>19331647</pmid><doi>10.1042/CS20080654</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical science (1979), 2009-11, Vol.117 (9-10), p.339-344 |
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| subjects | Adult Aged Amino Acids - metabolism Biological and medical sciences Cardiology. Vascular system Female Gastroenterology. Liver. Pancreas. Abdomen General aspects Glutathione - metabolism Hepatectomy Humans Intraoperative Care - methods Lactic Acid - metabolism Liver - metabolism Liver Neoplasms - secondary Liver Neoplasms - surgery Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Middle Aged Other diseases. Semiology Oxidation-Reduction Oxidative Stress Pilot Projects Reperfusion Injury - metabolism Time Factors Warm Ischemia |
| title | Glutathione and amino acid concentrations in human liver during short warm ischaemia and reperfusion: a pilot study |
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