Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda
During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty-seven of them had tuberculosis-like lesions and tissue samples were cultured. Only 17 cultures yielded acid-fast bacilli; 11 of them were confirmed as Mycobacterium bovis and...
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| Veröffentlicht in: | Veterinary record 2009-05, Vol.164 (21), p.655-658 |
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| creator | Asiimwe, B. B. Asiimwe, J. Kallenius, G. Ashaba, F. K. Ghebremichael, S. Joloba, M. Koivula, T. |
| description | During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty-seven of them had tuberculosis-like lesions and tissue samples were cultured. Only 17 cultures yielded acid-fast bacilli; 11 of them were confirmed as Mycobacterium bovis and six as non-tuberculous mycobacteria (NTM). GenoType Mycobacterium assays on the six NTM identified two as Mycobacterium fortuitum and one as Mycobacterium intracellulare, but three were unidentified. Characterisation of the M bovis isolates by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) revealed that five of the six spoligotype patterns observed in the 11 strains had not been previously reported, and seven of the nine isolates typed by RFLP had multicopy number IS6110 patterns. Six of the 11 infected carcases had multiple sites of infection, but none was condemned as unfit for human consumption. |
| doi_str_mv | 10.1136/vr.164.21.655 |
| format | Article |
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B. ; Asiimwe, J. ; Kallenius, G. ; Ashaba, F. K. ; Ghebremichael, S. ; Joloba, M. ; Koivula, T.</creator><creatorcontrib>Asiimwe, B. B. ; Asiimwe, J. ; Kallenius, G. ; Ashaba, F. K. ; Ghebremichael, S. ; Joloba, M. ; Koivula, T.</creatorcontrib><description>During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty-seven of them had tuberculosis-like lesions and tissue samples were cultured. Only 17 cultures yielded acid-fast bacilli; 11 of them were confirmed as Mycobacterium bovis and six as non-tuberculous mycobacteria (NTM). GenoType Mycobacterium assays on the six NTM identified two as Mycobacterium fortuitum and one as Mycobacterium intracellulare, but three were unidentified. Characterisation of the M bovis isolates by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) revealed that five of the six spoligotype patterns observed in the 11 strains had not been previously reported, and seven of the nine isolates typed by RFLP had multicopy number IS6110 patterns. Six of the 11 infected carcases had multiple sites of infection, but none was condemned as unfit for human consumption.</description><identifier>ISSN: 0042-4900</identifier><identifier>EISSN: 2042-7670</identifier><identifier>DOI: 10.1136/vr.164.21.655</identifier><identifier>PMID: 19465755</identifier><language>eng</language><publisher>England: BMJ Publishing Group Limited</publisher><subject>Abattoirs ; Animals ; beef carcasses ; Cattle ; Cattle Diseases - microbiology ; Caustic soda ; Developing countries ; DNA, Bacterial - chemistry ; Food Contamination - analysis ; genetic techniques and protocols ; Genotype ; Genotype & phenotype ; LDCs ; Meat - microbiology ; Medicin och hälsovetenskap ; Mycobacterium - classification ; Mycobacterium - genetics ; Mycobacterium - isolation & purification ; Mycobacterium bovis ; Mycobacterium bovis - classification ; Mycobacterium bovis - genetics ; Mycobacterium bovis - isolation & purification ; Mycobacterium Infections, Nontuberculous - microbiology ; Mycobacterium Infections, Nontuberculous - veterinary ; Nomads ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; Software ; Tuberculosis ; Tuberculosis, Bovine - microbiology ; Uganda</subject><ispartof>Veterinary record, 2009-05, Vol.164 (21), p.655-658</ispartof><rights>British Veterinary Association. All rights reserved.</rights><rights>British Veterinary Association 2009</rights><rights>Copyright: 2009 British Veterinary Association. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b5110-7d165da0ed61fb6aee1ca58819dc415c8145a555c02ef720b64a9919953c48903</citedby><cites>FETCH-LOGICAL-b5110-7d165da0ed61fb6aee1ca58819dc415c8145a555c02ef720b64a9919953c48903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1136%2Fvr.164.21.655$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1136%2Fvr.164.21.655$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19465755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:118880102$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Asiimwe, B. B.</creatorcontrib><creatorcontrib>Asiimwe, J.</creatorcontrib><creatorcontrib>Kallenius, G.</creatorcontrib><creatorcontrib>Ashaba, F. K.</creatorcontrib><creatorcontrib>Ghebremichael, S.</creatorcontrib><creatorcontrib>Joloba, M.</creatorcontrib><creatorcontrib>Koivula, T.</creatorcontrib><title>Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda</title><title>Veterinary record</title><addtitle>Vet Rec</addtitle><description>During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty-seven of them had tuberculosis-like lesions and tissue samples were cultured. Only 17 cultures yielded acid-fast bacilli; 11 of them were confirmed as Mycobacterium bovis and six as non-tuberculous mycobacteria (NTM). GenoType Mycobacterium assays on the six NTM identified two as Mycobacterium fortuitum and one as Mycobacterium intracellulare, but three were unidentified. Characterisation of the M bovis isolates by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) revealed that five of the six spoligotype patterns observed in the 11 strains had not been previously reported, and seven of the nine isolates typed by RFLP had multicopy number IS6110 patterns. Six of the 11 infected carcases had multiple sites of infection, but none was condemned as unfit for human consumption.</description><subject>Abattoirs</subject><subject>Animals</subject><subject>beef carcasses</subject><subject>Cattle</subject><subject>Cattle Diseases - microbiology</subject><subject>Caustic soda</subject><subject>Developing countries</subject><subject>DNA, Bacterial - chemistry</subject><subject>Food Contamination - analysis</subject><subject>genetic techniques and protocols</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>LDCs</subject><subject>Meat - microbiology</subject><subject>Medicin och hälsovetenskap</subject><subject>Mycobacterium - classification</subject><subject>Mycobacterium - genetics</subject><subject>Mycobacterium - isolation & purification</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium bovis - classification</subject><subject>Mycobacterium bovis - genetics</subject><subject>Mycobacterium bovis - isolation & purification</subject><subject>Mycobacterium Infections, Nontuberculous - microbiology</subject><subject>Mycobacterium Infections, Nontuberculous - veterinary</subject><subject>Nomads</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Software</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Bovine - microbiology</subject><subject>Uganda</subject><issn>0042-4900</issn><issn>2042-7670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkc2P0zAQxS0EYsvCkStY4sIlxeP6Iz7CaheQdoUEW67WxHHalKQudtJV_3tcpdATcJrR6PfGb_wIeQlsDrBQ7_ZxDkrMOcyVlI_IjDPBC600e0xm7NgLw9gFeZbShjFu5II_JRdghJJayhmJd6HzbuwwUrfGiG7wsU04tGFLQ0PvDi5U03DsaRX2baJtCh0OPtEmhp46HIbO5xIdpjzEgSJ17XCgqcNxtc7SdRiTp-2WLle4rfE5edJgl_yLU70ky5vr-6tPxe2Xj5-v3t8WlQRgha5ByRqZrxU0lULvwaEsSzC1EyBdCUKilNIx7hvNWaUEGgMmn-hEadjikhTT3vTgd2Nld7HtMR5swNaeRj9y562U2oDMvPkrv4uhPot-CwHKsmTAeNa-mbQZ_Dn6NNhNGOM2n2dBayOzYSjPjlwMKUXf_HkDmD2maffR5jQtB5vTzPyr09ax6n19pk_xZUBNwEPb-cO_t9nv1_dfP9xw4OL4N68nYYPB4ipHbpffOIMFA7XQmotMvJ2Iqt_8x-Yvv53DcA</recordid><startdate>20090523</startdate><enddate>20090523</enddate><creator>Asiimwe, B. 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K. ; Ghebremichael, S. ; Joloba, M. ; Koivula, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b5110-7d165da0ed61fb6aee1ca58819dc415c8145a555c02ef720b64a9919953c48903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Abattoirs</topic><topic>Animals</topic><topic>beef carcasses</topic><topic>Cattle</topic><topic>Cattle Diseases - microbiology</topic><topic>Caustic soda</topic><topic>Developing countries</topic><topic>DNA, Bacterial - chemistry</topic><topic>Food Contamination - analysis</topic><topic>genetic techniques and protocols</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>LDCs</topic><topic>Meat - microbiology</topic><topic>Medicin och hälsovetenskap</topic><topic>Mycobacterium - classification</topic><topic>Mycobacterium - genetics</topic><topic>Mycobacterium - isolation & purification</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium bovis - classification</topic><topic>Mycobacterium bovis - genetics</topic><topic>Mycobacterium bovis - isolation & purification</topic><topic>Mycobacterium Infections, Nontuberculous - microbiology</topic><topic>Mycobacterium Infections, Nontuberculous - veterinary</topic><topic>Nomads</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Software</topic><topic>Tuberculosis</topic><topic>Tuberculosis, Bovine - microbiology</topic><topic>Uganda</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asiimwe, B. 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B.</au><au>Asiimwe, J.</au><au>Kallenius, G.</au><au>Ashaba, F. K.</au><au>Ghebremichael, S.</au><au>Joloba, M.</au><au>Koivula, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda</atitle><jtitle>Veterinary record</jtitle><addtitle>Vet Rec</addtitle><date>2009-05-23</date><risdate>2009</risdate><volume>164</volume><issue>21</issue><spage>655</spage><epage>658</epage><pages>655-658</pages><issn>0042-4900</issn><eissn>2042-7670</eissn><abstract>During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty-seven of them had tuberculosis-like lesions and tissue samples were cultured. Only 17 cultures yielded acid-fast bacilli; 11 of them were confirmed as Mycobacterium bovis and six as non-tuberculous mycobacteria (NTM). GenoType Mycobacterium assays on the six NTM identified two as Mycobacterium fortuitum and one as Mycobacterium intracellulare, but three were unidentified. Characterisation of the M bovis isolates by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) revealed that five of the six spoligotype patterns observed in the 11 strains had not been previously reported, and seven of the nine isolates typed by RFLP had multicopy number IS6110 patterns. Six of the 11 infected carcases had multiple sites of infection, but none was condemned as unfit for human consumption.</abstract><cop>England</cop><pub>BMJ Publishing Group Limited</pub><pmid>19465755</pmid><doi>10.1136/vr.164.21.655</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Abattoirs Animals beef carcasses Cattle Cattle Diseases - microbiology Caustic soda Developing countries DNA, Bacterial - chemistry Food Contamination - analysis genetic techniques and protocols Genotype Genotype & phenotype LDCs Meat - microbiology Medicin och hälsovetenskap Mycobacterium - classification Mycobacterium - genetics Mycobacterium - isolation & purification Mycobacterium bovis Mycobacterium bovis - classification Mycobacterium bovis - genetics Mycobacterium bovis - isolation & purification Mycobacterium Infections, Nontuberculous - microbiology Mycobacterium Infections, Nontuberculous - veterinary Nomads Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Software Tuberculosis Tuberculosis, Bovine - microbiology Uganda |
| title | Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda |
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