Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy i...

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Veröffentlicht in:OPTICS EXPRESS 2007-04, Vol.15 (9), p.5366-5375
Hauptverfasser: Hassler, Kai, Rigler, Per, Blom, Hans, Rigler, Rudolf, Widengren, Jerker, Lasser, Theo
Format: Artikel
Sprache:eng
Schlagworte:
diffusion
enzyme
fluctuations
glycol
high count-rate
Medicin och hälsovetenskap
molecules
proteins
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container_issue 9
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container_title OPTICS EXPRESS
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creator Hassler, Kai
Rigler, Per
Blom, Hans
Rigler, Rudolf
Widengren, Jerker
Lasser, Theo
description This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.
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subjects diffusion
enzyme
fluctuations
glycol
high count-rate
Medicin och hälsovetenskap
molecules
proteins
title Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy
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