Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells

There are many reports of defined culture systems for the propagation of human embryonic stem cells in the absence of feeder cell support, but no previous study has undertaken a multi-laboratory comparison of these diverse methodologies. In this study, five separate laboratories, each with experienc...

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Veröffentlicht in:	In vitro cellular & developmental biology. Animal 2010-04, Vol.46 (3-4), p.247-258
Hauptverfasser:	Akopian, Veronika, Andrews, Peter W, Beil, Stephen, Benvenisty, Nissim, Brehm, Jennifer, Christie, Megan, Ford, Angela, Fox, Victoria, Gokhale, Paul J, Healy, Lyn, Holm, Frida, Hovatta, Outi, Knowles, Barbara B, Ludwig, Tenneille E, McKay, Ronald D. G, Miyazaki, Takamichi, Nakatsuji, Norio, Oh, Steve K. W, Pera, Martin F, Rossant, Janet, Stacey, Glyn N, Suemori, Hirofumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptations Animal Genetics and Genomics Animals Biomedical and Life Sciences Cell Adhesion Cell Biology Cell Count Cell Culture Cell culture techniques Cell Culture Techniques - methods Cell death Cell growth Cell Line Cell lines Cell Proliferation Cell Survival Cultured cells Cytology Developmental Biology Differentiation Embryo cells Embryo fibroblasts Embryonic stem cells Embryonic Stem Cells - cytology epigenetics Feeder cells Fibroblast growth factor 2 Flow Cytometry Growth factors Human growth Humans Life Sciences Media (culture) Mice Microscopy Pluripotent stem cells Quality control Stem Cells Surface markers
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