High-Density Lipoprotein Inhibits the Uptake of Modified Low- Density Lipoprotein and the Expression of CD36 and FcγRI

Aim: Modified low-density lipoprotein (mLDL), mainly upon oxidative and enzymatic modification, is the major atherogenic lipoprotein. Conversely, high-density lipoprotein (HDL) is considered antiatherogenic because of its ability to remove cholesterol. The aim of this work was to analyze both the in...

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Veröffentlicht in:Journal of Atherosclerosis and Thrombosis 2010, Vol.17(8), pp.844-857
Hauptverfasser: Carvalho, Márcia Dias Teixeira, Vendrame, Célia Maria Vieira, Ketelhuth, Daniel Francisco Jacon, Yamashiro-Kanashiro, Edite Hatsumi, Goto, Hiro, Gidlund, Magnus
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container_end_page 857
container_issue 8
container_start_page 844
container_title Journal of Atherosclerosis and Thrombosis
container_volume 17
creator Carvalho, Márcia Dias Teixeira
Vendrame, Célia Maria Vieira
Ketelhuth, Daniel Francisco Jacon
Yamashiro-Kanashiro, Edite Hatsumi
Goto, Hiro
Gidlund, Magnus
description Aim: Modified low-density lipoprotein (mLDL), mainly upon oxidative and enzymatic modification, is the major atherogenic lipoprotein. Conversely, high-density lipoprotein (HDL) is considered antiatherogenic because of its ability to remove cholesterol. The aim of this work was to analyze both the influence of HDL on the uptake of mLDL and the expression of CD36 and Fcγ I receptors on monocytic cell lines during cell differentiation. Methods: Uptake of fluorescein isothiocyanate (FITC)-conjugated LDL and FITC-conjugated mLDL, i.e., copper-oxidized LDL (oxLDL) or trypsin enzyme modified LDL (enzLDL), was analyzed, as well as the expression of CD36 and FcγRI in THP-1 and U937 cells, using flow cytometry. Results: HDL inhibited the uptake of mLDL, which varied in degree depending on the cell line or type of mLDL. Further, HDL rapidly decreased CD36 and FcγRI involved in the uptake of mLDL. Conclusions: We demonstrate that modified LDL promotes specific LDL receptor-independent uptake by monocytic cell lines, and that the uptake of LDL and enzLDL is less than that of oxLDL. In this process, HDL diminishes the uptake of LDL or mLDL, which may involve the down-regulation of receptors (CD36 and Fcγ I). This regulatory process represents another way by which HDL can be anti-atherogenic and it depends on the type of modification of LDL and the stage of differentiation of monocytes to macrophages.
doi_str_mv 10.5551/jat.3905
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Conversely, high-density lipoprotein (HDL) is considered antiatherogenic because of its ability to remove cholesterol. The aim of this work was to analyze both the influence of HDL on the uptake of mLDL and the expression of CD36 and Fcγ I receptors on monocytic cell lines during cell differentiation. Methods: Uptake of fluorescein isothiocyanate (FITC)-conjugated LDL and FITC-conjugated mLDL, i.e., copper-oxidized LDL (oxLDL) or trypsin enzyme modified LDL (enzLDL), was analyzed, as well as the expression of CD36 and FcγRI in THP-1 and U937 cells, using flow cytometry. Results: HDL inhibited the uptake of mLDL, which varied in degree depending on the cell line or type of mLDL. Further, HDL rapidly decreased CD36 and FcγRI involved in the uptake of mLDL. Conclusions: We demonstrate that modified LDL promotes specific LDL receptor-independent uptake by monocytic cell lines, and that the uptake of LDL and enzLDL is less than that of oxLDL. In this process, HDL diminishes the uptake of LDL or mLDL, which may involve the down-regulation of receptors (CD36 and Fcγ I). This regulatory process represents another way by which HDL can be anti-atherogenic and it depends on the type of modification of LDL and the stage of differentiation of monocytes to macrophages.</description><identifier>ISSN: 1340-3478</identifier><identifier>EISSN: 1880-3873</identifier><identifier>DOI: 10.5551/jat.3905</identifier><language>eng</language><publisher>Japan Atherosclerosis Society</publisher><subject>Atherosclerosis ; Foam cells ; Lipids ; Lipoproteins ; Macrophages</subject><ispartof>Journal of Atherosclerosis and Thrombosis, 2010, Vol.17(8), pp.844-857</ispartof><rights>2010 Japan Atherosclerosis Society</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-e13974ef62a3b32294681d02e8bda03835b8b25cfa5a55e180d35ca1f4eb2cb3</citedby><cites>FETCH-LOGICAL-c444t-e13974ef62a3b32294681d02e8bda03835b8b25cfa5a55e180d35ca1f4eb2cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,552,780,784,885,1883,27924,27925</link.rule.ids><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:121434101$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Carvalho, Márcia Dias Teixeira</creatorcontrib><creatorcontrib>Vendrame, Célia Maria Vieira</creatorcontrib><creatorcontrib>Ketelhuth, Daniel Francisco Jacon</creatorcontrib><creatorcontrib>Yamashiro-Kanashiro, Edite Hatsumi</creatorcontrib><creatorcontrib>Goto, Hiro</creatorcontrib><creatorcontrib>Gidlund, Magnus</creatorcontrib><title>High-Density Lipoprotein Inhibits the Uptake of Modified Low- Density Lipoprotein and the Expression of CD36 and FcγRI</title><title>Journal of Atherosclerosis and Thrombosis</title><addtitle>JAT</addtitle><description>Aim: Modified low-density lipoprotein (mLDL), mainly upon oxidative and enzymatic modification, is the major atherogenic lipoprotein. 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subjects Atherosclerosis
Foam cells
Lipids
Lipoproteins
Macrophages
title High-Density Lipoprotein Inhibits the Uptake of Modified Low- Density Lipoprotein and the Expression of CD36 and FcγRI
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