Retinoic acid-inducible gene 1-like receptors in the upper respiratory tract
Retinoic acid-inducible gene 1-like receptors (RLRs) are a novel family of pattern recognition receptors that include retinoic acid-inducible gene 1 (RIG-1), melanoma differentiation-associated gene 5 (MDA-5), and laboratory of genomics and physiology 2 (LGP-2). The knowledge of RLRs and their funct...
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| Veröffentlicht in: | American journal of rhinology & allergy 2011-11, Vol.25 (6), p.e262-e267 |
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| container_title | American journal of rhinology & allergy |
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| creator | Bogefors, Jesper Kvarnhammar, Anne Månsson Latif, Leith Petterson, Terese Uddman, Rolf Cardell, Lars Olaf |
| description | Retinoic acid-inducible gene 1-like receptors (RLRs) are a novel family of pattern recognition receptors that include retinoic acid-inducible gene 1 (RIG-1), melanoma differentiation-associated gene 5 (MDA-5), and laboratory of genomics and physiology 2 (LGP-2). The knowledge of RLRs and their function in the human airway is limited. This study explores the role of RLRs in the upper respiratory tract.
Tonsils, adenoids, nasal polyps, and biopsy specimens from the nasal mucosa were examined for the occurrence of the RIG-1, MDA-5, and LGP-2 using real-time reverse-transcription polymerase chain reaction and immunohistochemistry. The nasopharyngeal epithelial cell line FaDu was cultured with the RIG-1/MDA-5 ligand poly(I:C)/LyoVec (Invivogen, San Diego, CA) and analyzed for cytokine release using ELISA.
RIG-1, MDA-5, and LGP-2 mRNA were found in all tissues tested. The airway epithelium appeared to be their most prominent location. The RIG-1 and MDA-5 mRNA levels were higher in nasal polyps than in normal nasal mucosa, a state that seemed to be reversed by local steroid treatment. Culture of FaDu with poly(I:C)/LyoVec resulted in IL-6 and IL-8 release. No alteration in RLR expression in tonsils was seen on infection.
This study shows the presence and functional activity of RLRs in the human upper airways. It also suggests a role for RLRs in nasal polyposis. |
| doi_str_mv | 10.2500/ajra.2011.25.3712 |
| format | Article |
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Tonsils, adenoids, nasal polyps, and biopsy specimens from the nasal mucosa were examined for the occurrence of the RIG-1, MDA-5, and LGP-2 using real-time reverse-transcription polymerase chain reaction and immunohistochemistry. The nasopharyngeal epithelial cell line FaDu was cultured with the RIG-1/MDA-5 ligand poly(I:C)/LyoVec (Invivogen, San Diego, CA) and analyzed for cytokine release using ELISA.
RIG-1, MDA-5, and LGP-2 mRNA were found in all tissues tested. The airway epithelium appeared to be their most prominent location. The RIG-1 and MDA-5 mRNA levels were higher in nasal polyps than in normal nasal mucosa, a state that seemed to be reversed by local steroid treatment. Culture of FaDu with poly(I:C)/LyoVec resulted in IL-6 and IL-8 release. No alteration in RLR expression in tonsils was seen on infection.
This study shows the presence and functional activity of RLRs in the human upper airways. It also suggests a role for RLRs in nasal polyposis.</description><identifier>ISSN: 1945-8924</identifier><identifier>EISSN: 1945-8932</identifier><identifier>DOI: 10.2500/ajra.2011.25.3712</identifier><identifier>PMID: 22185736</identifier><language>eng</language><publisher>United States</publisher><subject>Adenoids - pathology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biopsy ; Cell Line ; Child ; Child, Preschool ; Clinical Medicine ; DEAD Box Protein 58 ; DEAD-box RNA Helicases - genetics ; DEAD-box RNA Helicases - metabolism ; Female ; Gene Expression Regulation ; Humans ; Immunohistochemistry ; Infant ; Interferon-Induced Helicase, IFIH1 ; Interleukin-6 - metabolism ; Interleukin-8 - metabolism ; Klinisk medicin ; Male ; Medical and Health Sciences ; Medicin och hälsovetenskap ; Middle Aged ; Nasal Mucosa - drug effects ; Nasal Mucosa - immunology ; Nasal Mucosa - metabolism ; Nasal Mucosa - pathology ; Nasal Polyps - genetics ; Nasal Polyps - immunology ; Nasal Polyps - metabolism ; Nasal Polyps - pathology ; Oto-rhino-laryngologi ; Otorhinolaryngology ; Palatine Tonsil - pathology ; Poly I-C - pharmacology ; Receptors, Immunologic ; Receptors, Pattern Recognition - genetics ; Receptors, Pattern Recognition - metabolism ; RNA Helicases - genetics ; RNA Helicases - metabolism ; Young Adult</subject><ispartof>American journal of rhinology & allergy, 2011-11, Vol.25 (6), p.e262-e267</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-f61c6ea9ec79c87e81fb9028f85207664d554bf02f0f961262b318f5107b9b933</citedby><cites>FETCH-LOGICAL-c407t-f61c6ea9ec79c87e81fb9028f85207664d554bf02f0f961262b318f5107b9b933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22185736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://lup.lub.lu.se/record/2273768$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:123740641$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Bogefors, Jesper</creatorcontrib><creatorcontrib>Kvarnhammar, Anne Månsson</creatorcontrib><creatorcontrib>Latif, Leith</creatorcontrib><creatorcontrib>Petterson, Terese</creatorcontrib><creatorcontrib>Uddman, Rolf</creatorcontrib><creatorcontrib>Cardell, Lars Olaf</creatorcontrib><title>Retinoic acid-inducible gene 1-like receptors in the upper respiratory tract</title><title>American journal of rhinology & allergy</title><addtitle>Am J Rhinol Allergy</addtitle><description>Retinoic acid-inducible gene 1-like receptors (RLRs) are a novel family of pattern recognition receptors that include retinoic acid-inducible gene 1 (RIG-1), melanoma differentiation-associated gene 5 (MDA-5), and laboratory of genomics and physiology 2 (LGP-2). The knowledge of RLRs and their function in the human airway is limited. This study explores the role of RLRs in the upper respiratory tract.
Tonsils, adenoids, nasal polyps, and biopsy specimens from the nasal mucosa were examined for the occurrence of the RIG-1, MDA-5, and LGP-2 using real-time reverse-transcription polymerase chain reaction and immunohistochemistry. The nasopharyngeal epithelial cell line FaDu was cultured with the RIG-1/MDA-5 ligand poly(I:C)/LyoVec (Invivogen, San Diego, CA) and analyzed for cytokine release using ELISA.
RIG-1, MDA-5, and LGP-2 mRNA were found in all tissues tested. The airway epithelium appeared to be their most prominent location. The RIG-1 and MDA-5 mRNA levels were higher in nasal polyps than in normal nasal mucosa, a state that seemed to be reversed by local steroid treatment. Culture of FaDu with poly(I:C)/LyoVec resulted in IL-6 and IL-8 release. No alteration in RLR expression in tonsils was seen on infection.
This study shows the presence and functional activity of RLRs in the human upper airways. It also suggests a role for RLRs in nasal polyposis.</description><subject>Adenoids - pathology</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biopsy</subject><subject>Cell Line</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Clinical Medicine</subject><subject>DEAD Box Protein 58</subject><subject>DEAD-box RNA Helicases - genetics</subject><subject>DEAD-box RNA Helicases - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Infant</subject><subject>Interferon-Induced Helicase, IFIH1</subject><subject>Interleukin-6 - metabolism</subject><subject>Interleukin-8 - metabolism</subject><subject>Klinisk medicin</subject><subject>Male</subject><subject>Medical and Health Sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Middle Aged</subject><subject>Nasal Mucosa - drug effects</subject><subject>Nasal Mucosa - immunology</subject><subject>Nasal Mucosa - metabolism</subject><subject>Nasal Mucosa - pathology</subject><subject>Nasal Polyps - genetics</subject><subject>Nasal Polyps - immunology</subject><subject>Nasal Polyps - metabolism</subject><subject>Nasal Polyps - pathology</subject><subject>Oto-rhino-laryngologi</subject><subject>Otorhinolaryngology</subject><subject>Palatine Tonsil - pathology</subject><subject>Poly I-C - pharmacology</subject><subject>Receptors, Immunologic</subject><subject>Receptors, Pattern Recognition - genetics</subject><subject>Receptors, Pattern Recognition - metabolism</subject><subject>RNA Helicases - genetics</subject><subject>RNA Helicases - metabolism</subject><subject>Young Adult</subject><issn>1945-8924</issn><issn>1945-8932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UcuO1DAQtBCIfX4AF5QbpwxuP-MjWgG70khICM6W7bTBu5kk2InQ_j2OZnZuHFr9qqpuqQh5B3THJKUf3WN2O0YBarvjGtgrcglGyLYznL0-10xckKtSHilVQgp4Sy4Yg05qri7J_jsuaZxSaFxIfZvGfg3JD9j8whEbaIf0hE3GgPMy5dKksVl-Y7POM-Y6LnPKri6emyW7sNyQN9ENBW9P-Zr8_PL5x919u__29eHu074NguqljQqCQmcwaBM6jR1EbyjrYicZ1UqJXkrhI2WRRqOAKeY5dFEC1d54w_k1aY-65S_Oq7dzTgeXn-3kkj2NnmqFVgpKQVf8_r_4YZ1r-Bobgdd7hmJlajBWRMFtB8FY46Tqe6j_UlnlPhzl5jz9WbEs9pBKwGFwI05rsQaYYIbJDQlHZMhTKRnj-TRQu1loNwvtZmFt7WZh5bw_qa_-gP2Z8eIZ_weL_JbX</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Bogefors, Jesper</creator><creator>Kvarnhammar, Anne Månsson</creator><creator>Latif, Leith</creator><creator>Petterson, Terese</creator><creator>Uddman, Rolf</creator><creator>Cardell, Lars Olaf</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D95</scope></search><sort><creationdate>20111101</creationdate><title>Retinoic acid-inducible gene 1-like receptors in the upper respiratory tract</title><author>Bogefors, Jesper ; Kvarnhammar, Anne Månsson ; Latif, Leith ; Petterson, Terese ; Uddman, Rolf ; Cardell, Lars Olaf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-f61c6ea9ec79c87e81fb9028f85207664d554bf02f0f961262b318f5107b9b933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenoids - pathology</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biopsy</topic><topic>Cell Line</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Clinical Medicine</topic><topic>DEAD Box Protein 58</topic><topic>DEAD-box RNA Helicases - genetics</topic><topic>DEAD-box RNA Helicases - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Infant</topic><topic>Interferon-Induced Helicase, IFIH1</topic><topic>Interleukin-6 - metabolism</topic><topic>Interleukin-8 - metabolism</topic><topic>Klinisk medicin</topic><topic>Male</topic><topic>Medical and Health Sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Middle Aged</topic><topic>Nasal Mucosa - drug effects</topic><topic>Nasal Mucosa - immunology</topic><topic>Nasal Mucosa - metabolism</topic><topic>Nasal Mucosa - pathology</topic><topic>Nasal Polyps - genetics</topic><topic>Nasal Polyps - immunology</topic><topic>Nasal Polyps - metabolism</topic><topic>Nasal Polyps - pathology</topic><topic>Oto-rhino-laryngologi</topic><topic>Otorhinolaryngology</topic><topic>Palatine Tonsil - pathology</topic><topic>Poly I-C - pharmacology</topic><topic>Receptors, Immunologic</topic><topic>Receptors, Pattern Recognition - genetics</topic><topic>Receptors, Pattern Recognition - metabolism</topic><topic>RNA Helicases - genetics</topic><topic>RNA Helicases - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bogefors, Jesper</creatorcontrib><creatorcontrib>Kvarnhammar, Anne Månsson</creatorcontrib><creatorcontrib>Latif, Leith</creatorcontrib><creatorcontrib>Petterson, Terese</creatorcontrib><creatorcontrib>Uddman, Rolf</creatorcontrib><creatorcontrib>Cardell, Lars Olaf</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Lunds universitet</collection><jtitle>American journal of rhinology & allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bogefors, Jesper</au><au>Kvarnhammar, Anne Månsson</au><au>Latif, Leith</au><au>Petterson, Terese</au><au>Uddman, Rolf</au><au>Cardell, Lars Olaf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retinoic acid-inducible gene 1-like receptors in the upper respiratory tract</atitle><jtitle>American journal of rhinology & allergy</jtitle><addtitle>Am J Rhinol Allergy</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>25</volume><issue>6</issue><spage>e262</spage><epage>e267</epage><pages>e262-e267</pages><issn>1945-8924</issn><eissn>1945-8932</eissn><abstract>Retinoic acid-inducible gene 1-like receptors (RLRs) are a novel family of pattern recognition receptors that include retinoic acid-inducible gene 1 (RIG-1), melanoma differentiation-associated gene 5 (MDA-5), and laboratory of genomics and physiology 2 (LGP-2). The knowledge of RLRs and their function in the human airway is limited. This study explores the role of RLRs in the upper respiratory tract.
Tonsils, adenoids, nasal polyps, and biopsy specimens from the nasal mucosa were examined for the occurrence of the RIG-1, MDA-5, and LGP-2 using real-time reverse-transcription polymerase chain reaction and immunohistochemistry. The nasopharyngeal epithelial cell line FaDu was cultured with the RIG-1/MDA-5 ligand poly(I:C)/LyoVec (Invivogen, San Diego, CA) and analyzed for cytokine release using ELISA.
RIG-1, MDA-5, and LGP-2 mRNA were found in all tissues tested. The airway epithelium appeared to be their most prominent location. The RIG-1 and MDA-5 mRNA levels were higher in nasal polyps than in normal nasal mucosa, a state that seemed to be reversed by local steroid treatment. Culture of FaDu with poly(I:C)/LyoVec resulted in IL-6 and IL-8 release. No alteration in RLR expression in tonsils was seen on infection.
This study shows the presence and functional activity of RLRs in the human upper airways. It also suggests a role for RLRs in nasal polyposis.</abstract><cop>United States</cop><pmid>22185736</pmid><doi>10.2500/ajra.2011.25.3712</doi></addata></record> |
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| language | eng |
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| source | MEDLINE; SAGE Complete; Alma/SFX Local Collection |
| subjects | Adenoids - pathology Adolescent Adult Aged Aged, 80 and over Biopsy Cell Line Child Child, Preschool Clinical Medicine DEAD Box Protein 58 DEAD-box RNA Helicases - genetics DEAD-box RNA Helicases - metabolism Female Gene Expression Regulation Humans Immunohistochemistry Infant Interferon-Induced Helicase, IFIH1 Interleukin-6 - metabolism Interleukin-8 - metabolism Klinisk medicin Male Medical and Health Sciences Medicin och hälsovetenskap Middle Aged Nasal Mucosa - drug effects Nasal Mucosa - immunology Nasal Mucosa - metabolism Nasal Mucosa - pathology Nasal Polyps - genetics Nasal Polyps - immunology Nasal Polyps - metabolism Nasal Polyps - pathology Oto-rhino-laryngologi Otorhinolaryngology Palatine Tonsil - pathology Poly I-C - pharmacology Receptors, Immunologic Receptors, Pattern Recognition - genetics Receptors, Pattern Recognition - metabolism RNA Helicases - genetics RNA Helicases - metabolism Young Adult |
| title | Retinoic acid-inducible gene 1-like receptors in the upper respiratory tract |
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