Single-copy assay quantification of HIV-1 RNA in paired cerebrospinal fluid and plasma samples from elite controllers
Elite controllers are a rare subset of HIV-1-infected individuals who maintain HIV-1 RNA concentrations in plasma below the lower limit of quantification of clinical assays (
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| Veröffentlicht in: | AIDS (London) 2013-04, Vol.27 (7), p.1145-1149 |
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| container_title | AIDS (London) |
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| creator | DAHL, Viktor PETERSON, Julia SPUDICH, Serena LEE, Evelyn SHACKLETT, Barbara L PRICE, Richard W PALMER, Sarah |
| description | Elite controllers are a rare subset of HIV-1-infected individuals who maintain HIV-1 RNA concentrations in plasma below the lower limit of quantification of clinical assays ( |
| doi_str_mv | 10.1097/QAD.0b013e32835cf235 |
| format | Article |
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We analysed paired cerebrospinal fluid (CSF) and plasma samples using a highly sensitive assay for HIV-1 RNA quantification.
We analysed 28 CSF samples and 27 concurrent plasma samples from 14 elite controllers with the highly sensitive single-copy assay (SCA) that allows for HIV-1 RNA quantification down to less than one copy of HIV-1 RNA per millilitre.
Three samples were excluded because of internal standard failure. HIV-1 RNA was detected in only five of 26 CSF samples compared with 14 of 26 plasma samples (P = 0.02), with a median of 0.2 (range, 0.1-6) copies/ml in CSF compared with 0.8 (range, 0.1-189 copies/ml) in plasma (P < 0.0001).
HIV-1 RNA could not be detected in CSF in most elite controllers using the highly sensitive SCA, and when detected, it was at significantly lower frequencies and concentrations than in plasma. Elite controllers thus control HIV-1 in the CNS very well. Whether the infrequent and small amounts of HIV-1 RNA in the CSF reflect production from a local reservoir or virion exchange between the blood and the CSF is uncertain.</description><identifier>ISSN: 0269-9370</identifier><identifier>ISSN: 1473-5571</identifier><identifier>EISSN: 1473-5571</identifier><identifier>DOI: 10.1097/QAD.0b013e32835cf235</identifier><identifier>PMID: 23211774</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Adult ; AIDS-Related Opportunistic Infections - blood ; AIDS-Related Opportunistic Infections - cerebrospinal fluid ; AIDS-Related Opportunistic Infections - immunology ; AIDS/HIV ; Biological and medical sciences ; Blood ; CD4 Lymphocyte Count ; CD4-Positive T-Lymphocytes ; CD8-Positive T-Lymphocytes ; Central Nervous System Viral Diseases - blood ; Central Nervous System Viral Diseases - cerebrospinal fluid ; Central Nervous System Viral Diseases - immunology ; Female ; HIV Infections - blood ; HIV Infections - cerebrospinal fluid ; HIV Infections - immunology ; HIV-1 - genetics ; HIV-1 - immunology ; Human immunodeficiency virus 1 ; Human viral diseases ; Humans ; Immunity, Innate ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Infectious diseases ; Male ; Medical sciences ; Medicin och hälsovetenskap ; Middle Aged ; RNA, Viral - blood ; RNA, Viral - cerebrospinal fluid ; RNA, Viral - immunology ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Viral Load ; Virion - immunology</subject><ispartof>AIDS (London), 2013-04, Vol.27 (7), p.1145-1149</ispartof><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-9506a3a8fa1de82600282713d96457ed55e993c5b88c422b1ff3e2ecdf848cf83</citedby><cites>FETCH-LOGICAL-c559t-9506a3a8fa1de82600282713d96457ed55e993c5b88c422b1ff3e2ecdf848cf83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,554,782,786,887,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27251495$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23211774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:126548717$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>DAHL, Viktor</creatorcontrib><creatorcontrib>PETERSON, Julia</creatorcontrib><creatorcontrib>SPUDICH, Serena</creatorcontrib><creatorcontrib>LEE, Evelyn</creatorcontrib><creatorcontrib>SHACKLETT, Barbara L</creatorcontrib><creatorcontrib>PRICE, Richard W</creatorcontrib><creatorcontrib>PALMER, Sarah</creatorcontrib><title>Single-copy assay quantification of HIV-1 RNA in paired cerebrospinal fluid and plasma samples from elite controllers</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>Elite controllers are a rare subset of HIV-1-infected individuals who maintain HIV-1 RNA concentrations in plasma below the lower limit of quantification of clinical assays (<20-50 copies/ml) in the absence of antiretroviral therapy. Here, we examine to what extent elite controllers also control infection of the central nervous system (CNS).
We analysed paired cerebrospinal fluid (CSF) and plasma samples using a highly sensitive assay for HIV-1 RNA quantification.
We analysed 28 CSF samples and 27 concurrent plasma samples from 14 elite controllers with the highly sensitive single-copy assay (SCA) that allows for HIV-1 RNA quantification down to less than one copy of HIV-1 RNA per millilitre.
Three samples were excluded because of internal standard failure. HIV-1 RNA was detected in only five of 26 CSF samples compared with 14 of 26 plasma samples (P = 0.02), with a median of 0.2 (range, 0.1-6) copies/ml in CSF compared with 0.8 (range, 0.1-189 copies/ml) in plasma (P < 0.0001).
HIV-1 RNA could not be detected in CSF in most elite controllers using the highly sensitive SCA, and when detected, it was at significantly lower frequencies and concentrations than in plasma. Elite controllers thus control HIV-1 in the CNS very well. Whether the infrequent and small amounts of HIV-1 RNA in the CSF reflect production from a local reservoir or virion exchange between the blood and the CSF is uncertain.</description><subject>Adult</subject><subject>AIDS-Related Opportunistic Infections - blood</subject><subject>AIDS-Related Opportunistic Infections - cerebrospinal fluid</subject><subject>AIDS-Related Opportunistic Infections - immunology</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>CD4 Lymphocyte Count</subject><subject>CD4-Positive T-Lymphocytes</subject><subject>CD8-Positive T-Lymphocytes</subject><subject>Central Nervous System Viral Diseases - blood</subject><subject>Central Nervous System Viral Diseases - cerebrospinal fluid</subject><subject>Central Nervous System Viral Diseases - immunology</subject><subject>Female</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - cerebrospinal fluid</subject><subject>HIV Infections - immunology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - immunology</subject><subject>Human immunodeficiency virus 1</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunity, Innate</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Middle Aged</subject><subject>RNA, Viral - blood</subject><subject>RNA, Viral - cerebrospinal fluid</subject><subject>RNA, Viral - immunology</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Viral Load</subject><subject>Virion - immunology</subject><issn>0269-9370</issn><issn>1473-5571</issn><issn>1473-5571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNqFkl1rFDEUhgdR7Fr9ByK5EbyZms9JciMsrdpCUfy8DZnMSY1mJtNkpmX_vbPstrWCeJWQPM9LDnmr6jnBRwRr-frT-uQIt5gwYFQx4Txl4kG1IlyyWghJHlYrTBtdaybxQfWklJ8YY4GVelwdUEYJkZKvqvlLGC4i1C6NG2RLsRt0OdthCj44O4U0oOTR6dn3mqDPH9YoDGi0IUOHHGRocypjGGxEPs6hQ3bo0Bht6S0qth8jFORz6hHEMAFyaZhyihFyeVo98jYWeLZfD6tv795-PT6tzz--Pzten9dOCD3VWuDGMqu8JR0o2mBMFZWEdbrhQkInBGjNnGiVcpzSlnjPgILrvOLKecUOq3qXW65hnFsz5tDbvDHJBrM_-rXswAhGdSMXXv-TH3Pq7qQbkdBGcCXJ1n2zcxegh87BMq2N9yPu3Qzhh7lIV4ZjzBvaLAGv9gE5Xc5QJtOH4iBGO0CaiyGcSEGlUuT_KBOUao7lNpXvULd8Vsngb19EsNkWySxFMn8XadFe_DnNrXTTnAV4uQdscTb6bAcXyh0nqSBcC_Yb1TfU-Q</recordid><startdate>20130424</startdate><enddate>20130424</enddate><creator>DAHL, Viktor</creator><creator>PETERSON, Julia</creator><creator>SPUDICH, Serena</creator><creator>LEE, Evelyn</creator><creator>SHACKLETT, Barbara L</creator><creator>PRICE, Richard W</creator><creator>PALMER, Sarah</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20130424</creationdate><title>Single-copy assay quantification of HIV-1 RNA in paired cerebrospinal fluid and plasma samples from elite controllers</title><author>DAHL, Viktor ; PETERSON, Julia ; SPUDICH, Serena ; LEE, Evelyn ; SHACKLETT, Barbara L ; PRICE, Richard W ; PALMER, Sarah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c559t-9506a3a8fa1de82600282713d96457ed55e993c5b88c422b1ff3e2ecdf848cf83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>AIDS-Related Opportunistic Infections - blood</topic><topic>AIDS-Related Opportunistic Infections - cerebrospinal fluid</topic><topic>AIDS-Related Opportunistic Infections - immunology</topic><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>CD4 Lymphocyte Count</topic><topic>CD4-Positive T-Lymphocytes</topic><topic>CD8-Positive T-Lymphocytes</topic><topic>Central Nervous System Viral Diseases - blood</topic><topic>Central Nervous System Viral Diseases - cerebrospinal fluid</topic><topic>Central Nervous System Viral Diseases - immunology</topic><topic>Female</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - cerebrospinal fluid</topic><topic>HIV Infections - immunology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - immunology</topic><topic>Human immunodeficiency virus 1</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunity, Innate</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Middle Aged</topic><topic>RNA, Viral - blood</topic><topic>RNA, Viral - cerebrospinal fluid</topic><topic>RNA, Viral - immunology</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Viral Load</topic><topic>Virion - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DAHL, Viktor</creatorcontrib><creatorcontrib>PETERSON, Julia</creatorcontrib><creatorcontrib>SPUDICH, Serena</creatorcontrib><creatorcontrib>LEE, Evelyn</creatorcontrib><creatorcontrib>SHACKLETT, Barbara L</creatorcontrib><creatorcontrib>PRICE, Richard W</creatorcontrib><creatorcontrib>PALMER, Sarah</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>AIDS (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DAHL, Viktor</au><au>PETERSON, Julia</au><au>SPUDICH, Serena</au><au>LEE, Evelyn</au><au>SHACKLETT, Barbara L</au><au>PRICE, Richard W</au><au>PALMER, Sarah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-copy assay quantification of HIV-1 RNA in paired cerebrospinal fluid and plasma samples from elite controllers</atitle><jtitle>AIDS (London)</jtitle><addtitle>AIDS</addtitle><date>2013-04-24</date><risdate>2013</risdate><volume>27</volume><issue>7</issue><spage>1145</spage><epage>1149</epage><pages>1145-1149</pages><issn>0269-9370</issn><issn>1473-5571</issn><eissn>1473-5571</eissn><abstract>Elite controllers are a rare subset of HIV-1-infected individuals who maintain HIV-1 RNA concentrations in plasma below the lower limit of quantification of clinical assays (<20-50 copies/ml) in the absence of antiretroviral therapy. Here, we examine to what extent elite controllers also control infection of the central nervous system (CNS).
We analysed paired cerebrospinal fluid (CSF) and plasma samples using a highly sensitive assay for HIV-1 RNA quantification.
We analysed 28 CSF samples and 27 concurrent plasma samples from 14 elite controllers with the highly sensitive single-copy assay (SCA) that allows for HIV-1 RNA quantification down to less than one copy of HIV-1 RNA per millilitre.
Three samples were excluded because of internal standard failure. HIV-1 RNA was detected in only five of 26 CSF samples compared with 14 of 26 plasma samples (P = 0.02), with a median of 0.2 (range, 0.1-6) copies/ml in CSF compared with 0.8 (range, 0.1-189 copies/ml) in plasma (P < 0.0001).
HIV-1 RNA could not be detected in CSF in most elite controllers using the highly sensitive SCA, and when detected, it was at significantly lower frequencies and concentrations than in plasma. Elite controllers thus control HIV-1 in the CNS very well. Whether the infrequent and small amounts of HIV-1 RNA in the CSF reflect production from a local reservoir or virion exchange between the blood and the CSF is uncertain.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>23211774</pmid><doi>10.1097/QAD.0b013e32835cf235</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0269-9370 |
| ispartof | AIDS (London), 2013-04, Vol.27 (7), p.1145-1149 |
| issn | 0269-9370 1473-5571 1473-5571 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_532967 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SWEPUB Freely available online; Journals@Ovid Ovid Autoload |
| subjects | Adult AIDS-Related Opportunistic Infections - blood AIDS-Related Opportunistic Infections - cerebrospinal fluid AIDS-Related Opportunistic Infections - immunology AIDS/HIV Biological and medical sciences Blood CD4 Lymphocyte Count CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Central Nervous System Viral Diseases - blood Central Nervous System Viral Diseases - cerebrospinal fluid Central Nervous System Viral Diseases - immunology Female HIV Infections - blood HIV Infections - cerebrospinal fluid HIV Infections - immunology HIV-1 - genetics HIV-1 - immunology Human immunodeficiency virus 1 Human viral diseases Humans Immunity, Innate Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Infectious diseases Male Medical sciences Medicin och hälsovetenskap Middle Aged RNA, Viral - blood RNA, Viral - cerebrospinal fluid RNA, Viral - immunology Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral Load Virion - immunology |
| title | Single-copy assay quantification of HIV-1 RNA in paired cerebrospinal fluid and plasma samples from elite controllers |
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