Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization
Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum) are common skin colonizers that are implicated as possible contributing factors in acne vulgaris development. We have established direct visualization tools for the simultaneous detection of these closely related spe...
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| Veröffentlicht in: | Anaerobe 2013-10, Vol.23, p.48-54 |
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| creator | Jahns, Anika C. Oprica, Cristina Vassilaki, Ismini Golovleva, Irina Palmer, Ruth H. Alexeyev, Oleg A. |
| description | Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum) are common skin colonizers that are implicated as possible contributing factors in acne vulgaris development. We have established direct visualization tools for the simultaneous detection of these closely related species with immunofluorescence assay and fluorescence in situ hybridization (FISH). As proof of principle, we were able to distinguish P. acnes and P. granulosum bacteria in multi-species populations in vitro as well as in a mock skin infection model upon labelling with 16S rRNA probes in combinatorial FISH as well as with antibodies. Furthermore, we report the co-localization of P. acnes and P. granulosum in the stratum corneum and hair follicles from patients with acne vulgaris as well as in healthy individuals. Further studies on the spatial distribution of these bacteria in skin structures in various skin disorders are needed.
•Direct visualization of Propionibacterium acnes and Propionibacterium granulosum in vitro and in skin tissue.•Differentiation of multi-species populations by 16S rRNA FISH and immunofluorescence.•Co-localization of P. acnes and P. granulosum in hair follicles in skin biopsies. |
| doi_str_mv | 10.1016/j.anaerobe.2013.07.002 |
| format | Article |
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•Direct visualization of Propionibacterium acnes and Propionibacterium granulosum in vitro and in skin tissue.•Differentiation of multi-species populations by 16S rRNA FISH and immunofluorescence.•Co-localization of P. acnes and P. granulosum in hair follicles in skin biopsies.</description><identifier>ISSN: 1075-9964</identifier><identifier>ISSN: 1095-8274</identifier><identifier>EISSN: 1095-8274</identifier><identifier>DOI: 10.1016/j.anaerobe.2013.07.002</identifier><identifier>PMID: 23896347</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>acne ; Acne Vulgaris - microbiology ; antibodies ; bacteria ; FISH ; fluorescence in situ hybridization ; fluorescent antibody technique ; Fluorescent Antibody Technique - methods ; Hair Follicle - microbiology ; hair follicles ; Humans ; In Situ Hybridization, Fluorescence ; Medicin och hälsovetenskap ; patients ; population ; Propionibacterium - classification ; Propionibacterium - genetics ; Propionibacterium acnes ; Propionibacterium granulosum ; Propionibacterium acnes ; Propionibacterium granulosum ; ribosomal RNA ; Skin ; Skin - microbiology</subject><ispartof>Anaerobe, 2013-10, Vol.23, p.48-54</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-fd81ca631de8dfc46a3b8f60cbeaab39214384ef86542fefc6a784e0ba22e6f23</citedby><cites>FETCH-LOGICAL-c550t-fd81ca631de8dfc46a3b8f60cbeaab39214384ef86542fefc6a784e0ba22e6f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anaerobe.2013.07.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23896347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-82026$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:127529716$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Jahns, Anika C.</creatorcontrib><creatorcontrib>Oprica, Cristina</creatorcontrib><creatorcontrib>Vassilaki, Ismini</creatorcontrib><creatorcontrib>Golovleva, Irina</creatorcontrib><creatorcontrib>Palmer, Ruth H.</creatorcontrib><creatorcontrib>Alexeyev, Oleg A.</creatorcontrib><title>Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization</title><title>Anaerobe</title><addtitle>Anaerobe</addtitle><description>Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum) are common skin colonizers that are implicated as possible contributing factors in acne vulgaris development. We have established direct visualization tools for the simultaneous detection of these closely related species with immunofluorescence assay and fluorescence in situ hybridization (FISH). As proof of principle, we were able to distinguish P. acnes and P. granulosum bacteria in multi-species populations in vitro as well as in a mock skin infection model upon labelling with 16S rRNA probes in combinatorial FISH as well as with antibodies. Furthermore, we report the co-localization of P. acnes and P. granulosum in the stratum corneum and hair follicles from patients with acne vulgaris as well as in healthy individuals. Further studies on the spatial distribution of these bacteria in skin structures in various skin disorders are needed.
•Direct visualization of Propionibacterium acnes and Propionibacterium granulosum in vitro and in skin tissue.•Differentiation of multi-species populations by 16S rRNA FISH and immunofluorescence.•Co-localization of P. acnes and P. granulosum in hair follicles in skin biopsies.</description><subject>acne</subject><subject>Acne Vulgaris - microbiology</subject><subject>antibodies</subject><subject>bacteria</subject><subject>FISH</subject><subject>fluorescence in situ hybridization</subject><subject>fluorescent antibody technique</subject><subject>Fluorescent Antibody Technique - methods</subject><subject>Hair Follicle - microbiology</subject><subject>hair follicles</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Medicin och hälsovetenskap</subject><subject>patients</subject><subject>population</subject><subject>Propionibacterium - classification</subject><subject>Propionibacterium - genetics</subject><subject>Propionibacterium acnes</subject><subject>Propionibacterium granulosum</subject><subject>Propionibacterium acnes</subject><subject>Propionibacterium granulosum</subject><subject>ribosomal RNA</subject><subject>Skin</subject><subject>Skin - microbiology</subject><issn>1075-9964</issn><issn>1095-8274</issn><issn>1095-8274</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkttu1DAQhiMEoqXwCiWXXJDF58R3VOUoVQKplFvLccZbL0m82HGr8h68L06zW4RE1SuPx98_Y3v-ojjGaIURFm82Kz1qCL6FFUGYrlC9Qog8Kg4xkrxqSM0ez3HNKykFOyiexbhBCGPG-dPigNBGCsrqw-L3uRtSP-kRfIrllYtJ9-6XnpwfS2_Lr8Fvc-habSYILg2lNiPEUo_df87WQY-p9zGH1266LN0wpNHbPvkA0cBo4Fb4T8KNZXRTKi9v2uC6XevnxROr-wgvdutRcfHh_bfTT9XZl4-fT0_OKsM5mirbNdhoQXEHTWcNE5q2jRXItKB1SyXBjDYMbCM4IxasEbrOe9RqQkBYQo-Kaqkbr2GbWrUNbtDhRnnt1C71I0egOKklmnl5L78Nvvsr2gsxqTmRNRZZ-_pe7Tv3_UT5sFZpSKohiMz4qwXPdX8miJMaXP6yvl9GpTBHPE-wlvxhlDHKMGGYZlQsqAk-xgD27hoYqdlYaqP2xlKzsRSqFbp9-fGuR2oH6O5keydl4OUCWO2VXgcX1cV5riAQQpxTITPxdiEgT_TKQVDRuNkDnQtgJtV599At_gBquvNt</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Jahns, Anika C.</creator><creator>Oprica, Cristina</creator><creator>Vassilaki, Ismini</creator><creator>Golovleva, Irina</creator><creator>Palmer, Ruth H.</creator><creator>Alexeyev, Oleg A.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D93</scope></search><sort><creationdate>20131001</creationdate><title>Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization</title><author>Jahns, Anika C. ; Oprica, Cristina ; Vassilaki, Ismini ; Golovleva, Irina ; Palmer, Ruth H. ; Alexeyev, Oleg A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-fd81ca631de8dfc46a3b8f60cbeaab39214384ef86542fefc6a784e0ba22e6f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>acne</topic><topic>Acne Vulgaris - microbiology</topic><topic>antibodies</topic><topic>bacteria</topic><topic>FISH</topic><topic>fluorescence in situ hybridization</topic><topic>fluorescent antibody technique</topic><topic>Fluorescent Antibody Technique - methods</topic><topic>Hair Follicle - microbiology</topic><topic>hair follicles</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Medicin och hälsovetenskap</topic><topic>patients</topic><topic>population</topic><topic>Propionibacterium - classification</topic><topic>Propionibacterium - genetics</topic><topic>Propionibacterium acnes</topic><topic>Propionibacterium granulosum</topic><topic>Propionibacterium acnes</topic><topic>Propionibacterium granulosum</topic><topic>ribosomal RNA</topic><topic>Skin</topic><topic>Skin - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jahns, Anika C.</creatorcontrib><creatorcontrib>Oprica, Cristina</creatorcontrib><creatorcontrib>Vassilaki, Ismini</creatorcontrib><creatorcontrib>Golovleva, Irina</creatorcontrib><creatorcontrib>Palmer, Ruth H.</creatorcontrib><creatorcontrib>Alexeyev, Oleg A.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Umeå universitet</collection><jtitle>Anaerobe</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jahns, Anika C.</au><au>Oprica, Cristina</au><au>Vassilaki, Ismini</au><au>Golovleva, Irina</au><au>Palmer, Ruth H.</au><au>Alexeyev, Oleg A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization</atitle><jtitle>Anaerobe</jtitle><addtitle>Anaerobe</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>23</volume><spage>48</spage><epage>54</epage><pages>48-54</pages><issn>1075-9964</issn><issn>1095-8274</issn><eissn>1095-8274</eissn><abstract>Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum) are common skin colonizers that are implicated as possible contributing factors in acne vulgaris development. We have established direct visualization tools for the simultaneous detection of these closely related species with immunofluorescence assay and fluorescence in situ hybridization (FISH). As proof of principle, we were able to distinguish P. acnes and P. granulosum bacteria in multi-species populations in vitro as well as in a mock skin infection model upon labelling with 16S rRNA probes in combinatorial FISH as well as with antibodies. Furthermore, we report the co-localization of P. acnes and P. granulosum in the stratum corneum and hair follicles from patients with acne vulgaris as well as in healthy individuals. Further studies on the spatial distribution of these bacteria in skin structures in various skin disorders are needed.
•Direct visualization of Propionibacterium acnes and Propionibacterium granulosum in vitro and in skin tissue.•Differentiation of multi-species populations by 16S rRNA FISH and immunofluorescence.•Co-localization of P. acnes and P. granulosum in hair follicles in skin biopsies.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23896347</pmid><doi>10.1016/j.anaerobe.2013.07.002</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | acne Acne Vulgaris - microbiology antibodies bacteria FISH fluorescence in situ hybridization fluorescent antibody technique Fluorescent Antibody Technique - methods Hair Follicle - microbiology hair follicles Humans In Situ Hybridization, Fluorescence Medicin och hälsovetenskap patients population Propionibacterium - classification Propionibacterium - genetics Propionibacterium acnes Propionibacterium granulosum Propionibacterium acnes Propionibacterium granulosum ribosomal RNA Skin Skin - microbiology |
| title | Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization |
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