Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection
Rotavirus-induced diarrhea is a life-threatening disease in immunocompromised individuals and in children in developing countries. We have developed a system for prophylaxis and therapy against rotavirus disease using transgenic rice expressing the neutralizing variable domain of a rotavirus-specifi...
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| Veröffentlicht in: | The Journal of clinical investigation 2013-09, Vol.123 (9), p.3829-3838 |
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| creator | Tokuhara, Daisuke Álvarez, Beatriz Mejima, Mio Hiroiwa, Tomoko Takahashi, Yuko Kurokawa, Shiho Kuroda, Masaharu Oyama, Masaaki Kozuka-Hata, Hiroko Nochi, Tomonori Sagara, Hiroshi Aladin, Farah Marcotte, Harold Frenken, Leon G J Iturriza-Gómara, Miren Kiyono, Hiroshi Hammarström, Lennart Yuki, Yoshikazu |
| description | Rotavirus-induced diarrhea is a life-threatening disease in immunocompromised individuals and in children in developing countries. We have developed a system for prophylaxis and therapy against rotavirus disease using transgenic rice expressing the neutralizing variable domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1). MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system and RNAi technology to suppress the production of major rice endogenous storage proteins. Orally administered MucoRice-ARP1 markedly decreased the viral load in immunocompetent and immunodeficient mice. The antibody retained in vitro neutralizing activity after long-term storage (>1 yr) and boiling and conferred protection in mice even after heat treatment at 94°C for 30 minutes. High-yield, water-soluble, and purification-free MucoRice-ARP1 thus forms the basis for orally administered prophylaxis and therapy against rotavirus infections. |
| doi_str_mv | 10.1172/jci70266 |
| format | Article |
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We have developed a system for prophylaxis and therapy against rotavirus disease using transgenic rice expressing the neutralizing variable domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1). MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system and RNAi technology to suppress the production of major rice endogenous storage proteins. Orally administered MucoRice-ARP1 markedly decreased the viral load in immunocompetent and immunodeficient mice. The antibody retained in vitro neutralizing activity after long-term storage (>1 yr) and boiling and conferred protection in mice even after heat treatment at 94°C for 30 minutes. High-yield, water-soluble, and purification-free MucoRice-ARP1 thus forms the basis for orally administered prophylaxis and therapy against rotavirus infections.</description><identifier>ISSN: 0021-9738</identifier><identifier>ISSN: 1558-8238</identifier><identifier>EISSN: 1558-8238</identifier><identifier>DOI: 10.1172/jci70266</identifier><identifier>PMID: 23925294</identifier><language>eng</language><publisher>United States: American Society for Clinical Investigation</publisher><subject><![CDATA[Administration, Oral ; Age ; Agricultural biotechnology ; Animals ; Antibiotic Prophylaxis ; Antibodies, Neutralizing - administration & dosage ; Antibodies, Neutralizing - biosynthesis ; Antibodies, Viral - administration & dosage ; Antibodies, Viral - biosynthesis ; Antiviral Agents - administration & dosage ; Biomedical research ; Care and treatment ; Clinical trials ; Diarrhea ; Diarrhea - immunology ; Diarrhea - prevention & control ; Diarrhea - virology ; Humans ; Industrialized nations ; Intestine, Small - pathology ; Intestine, Small - virology ; Medical research ; Medicin och hälsovetenskap ; Mice ; Mice, Inbred BALB C ; Mice, SCID ; Oryza - genetics ; Oryza - metabolism ; Oryza sativa ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Preventive medicine ; Protein Stability ; Proteins ; Rotavirus ; Rotavirus - immunology ; Rotavirus infections ; Rotavirus Infections - immunology ; Rotavirus Infections - prevention & control ; Rotavirus Infections - virology ; Scholarships & fellowships ; Science ; Seeds ; Seeds - genetics ; Seeds - metabolism ; Single-Chain Antibodies - administration & dosage ; Single-Chain Antibodies - biosynthesis ; Solubility ; Vaccines ; Virus Shedding ; Viruses ; Yeast]]></subject><ispartof>The Journal of clinical investigation, 2013-09, Vol.123 (9), p.3829-3838</ispartof><rights>COPYRIGHT 2013 American Society for Clinical Investigation</rights><rights>Copyright American Society for Clinical Investigation Sep 2013</rights><rights>Copyright © 2013, American Society for Clinical Investigation 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c830t-f8d802e89fef7a816984f2b1fa6bf142aa73bed707ac9e7a4c08f329253854113</citedby><cites>FETCH-LOGICAL-c830t-f8d802e89fef7a816984f2b1fa6bf142aa73bed707ac9e7a4c08f329253854113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754275/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754275/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,552,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23925294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:127407263$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Tokuhara, Daisuke</creatorcontrib><creatorcontrib>Álvarez, Beatriz</creatorcontrib><creatorcontrib>Mejima, Mio</creatorcontrib><creatorcontrib>Hiroiwa, Tomoko</creatorcontrib><creatorcontrib>Takahashi, Yuko</creatorcontrib><creatorcontrib>Kurokawa, Shiho</creatorcontrib><creatorcontrib>Kuroda, Masaharu</creatorcontrib><creatorcontrib>Oyama, Masaaki</creatorcontrib><creatorcontrib>Kozuka-Hata, Hiroko</creatorcontrib><creatorcontrib>Nochi, Tomonori</creatorcontrib><creatorcontrib>Sagara, Hiroshi</creatorcontrib><creatorcontrib>Aladin, Farah</creatorcontrib><creatorcontrib>Marcotte, Harold</creatorcontrib><creatorcontrib>Frenken, Leon G J</creatorcontrib><creatorcontrib>Iturriza-Gómara, Miren</creatorcontrib><creatorcontrib>Kiyono, Hiroshi</creatorcontrib><creatorcontrib>Hammarström, Lennart</creatorcontrib><creatorcontrib>Yuki, Yoshikazu</creatorcontrib><title>Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>Rotavirus-induced diarrhea is a life-threatening disease in immunocompromised individuals and in children in developing countries. We have developed a system for prophylaxis and therapy against rotavirus disease using transgenic rice expressing the neutralizing variable domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1). MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system and RNAi technology to suppress the production of major rice endogenous storage proteins. Orally administered MucoRice-ARP1 markedly decreased the viral load in immunocompetent and immunodeficient mice. The antibody retained in vitro neutralizing activity after long-term storage (>1 yr) and boiling and conferred protection in mice even after heat treatment at 94°C for 30 minutes. High-yield, water-soluble, and purification-free MucoRice-ARP1 thus forms the basis for orally administered prophylaxis and therapy against rotavirus infections.</description><subject>Administration, Oral</subject><subject>Age</subject><subject>Agricultural biotechnology</subject><subject>Animals</subject><subject>Antibiotic Prophylaxis</subject><subject>Antibodies, Neutralizing - administration & dosage</subject><subject>Antibodies, Neutralizing - biosynthesis</subject><subject>Antibodies, Viral - administration & dosage</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antiviral Agents - administration & dosage</subject><subject>Biomedical research</subject><subject>Care and treatment</subject><subject>Clinical trials</subject><subject>Diarrhea</subject><subject>Diarrhea - immunology</subject><subject>Diarrhea - prevention & control</subject><subject>Diarrhea - virology</subject><subject>Humans</subject><subject>Industrialized nations</subject><subject>Intestine, Small - 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administration & dosage</subject><subject>Single-Chain Antibodies - biosynthesis</subject><subject>Solubility</subject><subject>Vaccines</subject><subject>Virus Shedding</subject><subject>Viruses</subject><subject>Yeast</subject><issn>0021-9738</issn><issn>1558-8238</issn><issn>1558-8238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>D8T</sourceid><recordid>eNqNk11rFDEUhgdRbK2Cv0AGBNGLqfmcJDdCWfxYKRSq9TZkZk5ms85O1mSmdv-9WbvtdqSizEWG5HlfTt6Tk2XPMTrGWJC3y9oJRMryQXaIOZeFJFQ-zA4RIrhQgsqD7EmMS4QwY5w9zg4IVYQTxQ6zi3NXQ1GZCE3ug-ly0w-u8s0mt8G0K-iHfB38erHpzJWL6bTJhwUEs97kpjWuj0Me_GAuXRhj7noL9eB8_zR7ZE0X4dluPcouPrz_OvtUnJ59nM9OTotaUjQUVjYSEZDKghVG4lJJZkmFrSkrixkxRtAKGoGEqRUIw2okLSWpdio5w5geZcW1b_wJ67HS6-BWJmy0N07vtr6nP9CcCIFp4tVf-XTNZi-6EWIiGBKk3GrfXWsTsIKmTtGkvKYWk5PeLXTrLzUVnBHBk8HrnUHwP0aIg165WEPXmR78GDXmiKfLcaX-jTIiReqr3Jb18g906cfQp9QTxRSWimG1p1rTgU598qnEemuqTygveXowlO_jnFAt9Knhne_BurQ94Y_v4dPXwMrV9wreTASJGeBqaM0Yo55_Of9_9uzblH11h12A6YZF9N24fYtxCu6CrYOPMYC97R9GejtK-vNs_nuUEvribr9vwZvZob8A6U0Wnw</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Tokuhara, Daisuke</creator><creator>Álvarez, Beatriz</creator><creator>Mejima, Mio</creator><creator>Hiroiwa, Tomoko</creator><creator>Takahashi, Yuko</creator><creator>Kurokawa, Shiho</creator><creator>Kuroda, Masaharu</creator><creator>Oyama, Masaaki</creator><creator>Kozuka-Hata, Hiroko</creator><creator>Nochi, Tomonori</creator><creator>Sagara, Hiroshi</creator><creator>Aladin, Farah</creator><creator>Marcotte, Harold</creator><creator>Frenken, Leon G J</creator><creator>Iturriza-Gómara, Miren</creator><creator>Kiyono, Hiroshi</creator><creator>Hammarström, Lennart</creator><creator>Yuki, Yoshikazu</creator><general>American Society for Clinical Investigation</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>S0X</scope><scope>7X8</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20130901</creationdate><title>Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection</title><author>Tokuhara, Daisuke ; Álvarez, Beatriz ; Mejima, Mio ; Hiroiwa, Tomoko ; Takahashi, Yuko ; Kurokawa, Shiho ; Kuroda, Masaharu ; Oyama, Masaaki ; Kozuka-Hata, Hiroko ; Nochi, Tomonori ; Sagara, Hiroshi ; Aladin, Farah ; Marcotte, Harold ; Frenken, Leon G J ; Iturriza-Gómara, Miren ; Kiyono, Hiroshi ; Hammarström, Lennart ; Yuki, Yoshikazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c830t-f8d802e89fef7a816984f2b1fa6bf142aa73bed707ac9e7a4c08f329253854113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Administration, Oral</topic><topic>Age</topic><topic>Agricultural biotechnology</topic><topic>Animals</topic><topic>Antibiotic Prophylaxis</topic><topic>Antibodies, Neutralizing - administration & dosage</topic><topic>Antibodies, Neutralizing - biosynthesis</topic><topic>Antibodies, Viral - administration & dosage</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antiviral Agents - administration & dosage</topic><topic>Biomedical research</topic><topic>Care and treatment</topic><topic>Clinical trials</topic><topic>Diarrhea</topic><topic>Diarrhea - immunology</topic><topic>Diarrhea - prevention & control</topic><topic>Diarrhea - virology</topic><topic>Humans</topic><topic>Industrialized nations</topic><topic>Intestine, Small - pathology</topic><topic>Intestine, Small - virology</topic><topic>Medical research</topic><topic>Medicin och hälsovetenskap</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, SCID</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Preventive medicine</topic><topic>Protein Stability</topic><topic>Proteins</topic><topic>Rotavirus</topic><topic>Rotavirus - 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We have developed a system for prophylaxis and therapy against rotavirus disease using transgenic rice expressing the neutralizing variable domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1). MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system and RNAi technology to suppress the production of major rice endogenous storage proteins. Orally administered MucoRice-ARP1 markedly decreased the viral load in immunocompetent and immunodeficient mice. The antibody retained in vitro neutralizing activity after long-term storage (>1 yr) and boiling and conferred protection in mice even after heat treatment at 94°C for 30 minutes. High-yield, water-soluble, and purification-free MucoRice-ARP1 thus forms the basis for orally administered prophylaxis and therapy against rotavirus infections.</abstract><cop>United States</cop><pub>American Society for Clinical Investigation</pub><pmid>23925294</pmid><doi>10.1172/jci70266</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9738 |
| ispartof | The Journal of clinical investigation, 2013-09, Vol.123 (9), p.3829-3838 |
| issn | 0021-9738 1558-8238 1558-8238 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_527713 |
| source | MEDLINE; SWEPUB Freely available online; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Administration, Oral Age Agricultural biotechnology Animals Antibiotic Prophylaxis Antibodies, Neutralizing - administration & dosage Antibodies, Neutralizing - biosynthesis Antibodies, Viral - administration & dosage Antibodies, Viral - biosynthesis Antiviral Agents - administration & dosage Biomedical research Care and treatment Clinical trials Diarrhea Diarrhea - immunology Diarrhea - prevention & control Diarrhea - virology Humans Industrialized nations Intestine, Small - pathology Intestine, Small - virology Medical research Medicin och hälsovetenskap Mice Mice, Inbred BALB C Mice, SCID Oryza - genetics Oryza - metabolism Oryza sativa Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Preventive medicine Protein Stability Proteins Rotavirus Rotavirus - immunology Rotavirus infections Rotavirus Infections - immunology Rotavirus Infections - prevention & control Rotavirus Infections - virology Scholarships & fellowships Science Seeds Seeds - genetics Seeds - metabolism Single-Chain Antibodies - administration & dosage Single-Chain Antibodies - biosynthesis Solubility Vaccines Virus Shedding Viruses Yeast |
| title | Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T18%3A24%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rice-based%20oral%20antibody%20fragment%20prophylaxis%20and%20therapy%20against%20rotavirus%20infection&rft.jtitle=The%20Journal%20of%20clinical%20investigation&rft.au=Tokuhara,%20Daisuke&rft.date=2013-09-01&rft.volume=123&rft.issue=9&rft.spage=3829&rft.epage=3838&rft.pages=3829-3838&rft.issn=0021-9738&rft.eissn=1558-8238&rft_id=info:doi/10.1172/jci70266&rft_dat=%3Cgale_swepu%3EA356582335%3C/gale_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1449189419&rft_id=info:pmid/23925294&rft_galeid=A356582335&rfr_iscdi=true |