A multiple-alignment based primer design algorithm for genetically highly variable DNA targets

Primer design for highly variable DNA sequences is difficult, and experimental success requires attention to many interacting constraints. The advent of next-generation sequencing methods allows the investigation of rare variants otherwise hidden deep in large populations, but requires attention to...

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Veröffentlicht in:	BMC BIOINFORMATICS 2013-08, Vol.14 (1), p.255-255, Article 255
Hauptverfasser:	Brodin, Johanna, Krishnamoorthy, Mohan, Athreya, Gayathri, Fischer, Will, Hraber, Peter, Gleasner, Cheryl, Green, Lance, Korber, Bette, Leitner, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Amplicon sequencing BASIC BIOLOGICAL SCIENCES Bio-barcodes Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Computers Deoxyribonucleic acid Design DNA DNA Primers - genetics DNA sequencing Freeware Genetic algorithms Genetic testing Genomes High-Throughput Nucleotide Sequencing HIV HIV Infections - virology HIV-1 - genetics Human immunodeficiency virus Human immunodeficiency virus 1 Humans Mathematical & Computational Biology Methods Models, Genetic Multiplex National security Next-generation sequencing Nucleotide sequencing PCR Perl Polymerase Chain Reaction - methods Primer design Primer dimer Programming languages Sequence Analysis, DNA - methods Software Studies
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