Increased Tartrate-Resistant Acid Phosphatase Expression in Osteoblasts and Osteocytes in Experimental Osteoporosis in Rats
Tartrate-resistant acid phosphatase (TRAP) is known as an osteoclast marker, but osteoblasts and osteocytes in the vicinity of bone remodeling sites also express TRAP. Cell culture studies suggest that osteoblasts endocytose osteoclastic TRAP for inactivation. To evaluate whether changes in osteocla...
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| Veröffentlicht in: | Calcified tissue international 2014-05, Vol.94 (5), p.510-521 |
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| description | Tartrate-resistant acid phosphatase (TRAP) is known as an osteoclast marker, but osteoblasts and osteocytes in the vicinity of bone remodeling sites also express TRAP. Cell culture studies suggest that osteoblasts endocytose osteoclastic TRAP for inactivation. To evaluate whether changes in osteoclast activity could alter TRAP expression in osteoblasts and/or osteocytes in vivo, we studied the ovariectomized and vitamin D-deficient rat (Ovx-D) and rats healing from rickets. Bone sections were analyzed for TRAP gene expression by in situ hybridization, TRAP protein by immunogold labeling, and TRAP enzyme activity using the fluorescent substrate ELF97. Osteoblasts and osteocytes close to intracortical remodeling sites and bone surfaces demonstrated TRAP, most prominently in cancellous bone and osteocytes. Intracellular TRAP was located to electron-dense vesicles with similar morphology in both cell types. Ovx-D increased osteoclast activity (
p
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| doi_str_mv | 10.1007/s00223-013-9834-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_524566</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1514435066</sourcerecordid><originalsourceid>FETCH-LOGICAL-c657t-a50f867a29db907a8611785e1f1411561f0be1c48d97c921fd18f1e7be6985fe3</originalsourceid><addsrcrecordid>eNqNkl9r1TAYxoso7jj9AN5IwRtvonnzvzfCGFMHg8mY4F1I27c7nT1NTXKmwy9vas_mJgy8atrn9zzNmzxF8RLoW6BUv4uUMsYJBU4qwwXhj4oVCM4INUw_LlYUNJBK6a97xbMYLykFoZR6WuwxwSsJuloVv47HJqCL2JbnLqTgEpIzjH1MbkzlQdO35ee1j9PapQyVRz-ngDH2fiz7sTyNCX09uJhi6cZ2eW-uE8ZZzSyGfoNjcsMiTT74HD2LZy7F58WTzg0RX-ye-8WXD0fnh5_IyenH48ODE9IoqRNxknZGaceqtq6odkYBaCMROhAAUkFHa4RGmLbSTcWga8F0gLpGVRnZId8vyJIbf-C0re2Ud-XCtfWut7tP3_IKrWRCKpX56kF-Cr79a7oxAjPKAGc0e98v3gxssG3y9MEN9yPuKWO_thf-ygoQhnPIAW92AcF_32JMdtPHBofBjei30YIEpSVj6r9QIbikf0Z6_Q966bdhzKc-U8wwTsVMwUI1-Z5iwO5230Dt3Di7NM7mxtm5cZZnz6u7A986biqWAbY7zSyNFxju_PrB1N9oheSP</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1512823046</pqid></control><display><type>article</type><title>Increased Tartrate-Resistant Acid Phosphatase Expression in Osteoblasts and Osteocytes in Experimental Osteoporosis in Rats</title><source>MEDLINE</source><source>Springer Online Journals</source><source>SWEPUB Freely available online</source><creator>Solberg, Lene B. ; Brorson, Sverre-Henning ; Stordalen, Gunhild A. ; Bækkevold, Espen S. ; Andersson, Göran ; Reinholt, Finn P.</creator><creatorcontrib>Solberg, Lene B. ; Brorson, Sverre-Henning ; Stordalen, Gunhild A. ; Bækkevold, Espen S. ; Andersson, Göran ; Reinholt, Finn P.</creatorcontrib><description>Tartrate-resistant acid phosphatase (TRAP) is known as an osteoclast marker, but osteoblasts and osteocytes in the vicinity of bone remodeling sites also express TRAP. Cell culture studies suggest that osteoblasts endocytose osteoclastic TRAP for inactivation. To evaluate whether changes in osteoclast activity could alter TRAP expression in osteoblasts and/or osteocytes in vivo, we studied the ovariectomized and vitamin D-deficient rat (Ovx-D) and rats healing from rickets. Bone sections were analyzed for TRAP gene expression by in situ hybridization, TRAP protein by immunogold labeling, and TRAP enzyme activity using the fluorescent substrate ELF97. Osteoblasts and osteocytes close to intracortical remodeling sites and bone surfaces demonstrated TRAP, most prominently in cancellous bone and osteocytes. Intracellular TRAP was located to electron-dense vesicles with similar morphology in both cell types. Ovx-D increased osteoclast activity (
p
< 0.001) and ELF97
+
osteocytes (
p
< 0.05) in cancellous bone, but no corresponding increase was observed in the osteocyte lacunar area. The level of TRAP
+
vesicles in cortical osteoblasts (
p
< 0.01) in Ovx-D rats was also increased. Enhanced osteoclast activity was noted in healing rickets after 72 h (
p
< 0.05), but no differences in TRAP expression were detected in osteoblasts or osteocytes. Thus, increased osteoclast activity does not affect TRAP expression in osteoblasts and osteocytes, favoring the notion that increased TRAP in these cells is rather due to increased synthesis. Although the role of TRAP in osteoblasts and osteocytes remains elusive, we speculate that the function is related to the capability of the enzyme to regulate the phosphorylation of proteins known to be expressed by these cells.</description><identifier>ISSN: 0171-967X</identifier><identifier>ISSN: 1432-0827</identifier><identifier>EISSN: 1432-0827</identifier><identifier>DOI: 10.1007/s00223-013-9834-3</identifier><identifier>PMID: 24395179</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Acid Phosphatase - metabolism ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Bone Remodeling - physiology ; Bones ; Cell Biology ; Cell culture ; Disease Models, Animal ; Endocrinology ; Enzymes ; Female ; Fluorescent Antibody Technique ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Isoenzymes - metabolism ; Life Sciences ; Medicin och hälsovetenskap ; Microscopy, Electron, Transmission ; Original Research ; Orthopedics ; Osteoblasts - enzymology ; Osteoclasts - enzymology ; Osteocytes - enzymology ; Osteoporosis, Postmenopausal - enzymology ; Proteins ; Rats ; Rickets - enzymology ; Rodents ; Tartrate-Resistant Acid Phosphatase ; Vitamin D</subject><ispartof>Calcified tissue international, 2014-05, Vol.94 (5), p.510-521</ispartof><rights>The Author(s) 2014</rights><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c657t-a50f867a29db907a8611785e1f1411561f0be1c48d97c921fd18f1e7be6985fe3</citedby><cites>FETCH-LOGICAL-c657t-a50f867a29db907a8611785e1f1411561f0be1c48d97c921fd18f1e7be6985fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00223-013-9834-3$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00223-013-9834-3$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24395179$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:128681320$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Solberg, Lene B.</creatorcontrib><creatorcontrib>Brorson, Sverre-Henning</creatorcontrib><creatorcontrib>Stordalen, Gunhild A.</creatorcontrib><creatorcontrib>Bækkevold, Espen S.</creatorcontrib><creatorcontrib>Andersson, Göran</creatorcontrib><creatorcontrib>Reinholt, Finn P.</creatorcontrib><title>Increased Tartrate-Resistant Acid Phosphatase Expression in Osteoblasts and Osteocytes in Experimental Osteoporosis in Rats</title><title>Calcified tissue international</title><addtitle>Calcif Tissue Int</addtitle><addtitle>Calcif Tissue Int</addtitle><description>Tartrate-resistant acid phosphatase (TRAP) is known as an osteoclast marker, but osteoblasts and osteocytes in the vicinity of bone remodeling sites also express TRAP. Cell culture studies suggest that osteoblasts endocytose osteoclastic TRAP for inactivation. To evaluate whether changes in osteoclast activity could alter TRAP expression in osteoblasts and/or osteocytes in vivo, we studied the ovariectomized and vitamin D-deficient rat (Ovx-D) and rats healing from rickets. Bone sections were analyzed for TRAP gene expression by in situ hybridization, TRAP protein by immunogold labeling, and TRAP enzyme activity using the fluorescent substrate ELF97. Osteoblasts and osteocytes close to intracortical remodeling sites and bone surfaces demonstrated TRAP, most prominently in cancellous bone and osteocytes. Intracellular TRAP was located to electron-dense vesicles with similar morphology in both cell types. Ovx-D increased osteoclast activity (
p
< 0.001) and ELF97
+
osteocytes (
p
< 0.05) in cancellous bone, but no corresponding increase was observed in the osteocyte lacunar area. The level of TRAP
+
vesicles in cortical osteoblasts (
p
< 0.01) in Ovx-D rats was also increased. Enhanced osteoclast activity was noted in healing rickets after 72 h (
p
< 0.05), but no differences in TRAP expression were detected in osteoblasts or osteocytes. Thus, increased osteoclast activity does not affect TRAP expression in osteoblasts and osteocytes, favoring the notion that increased TRAP in these cells is rather due to increased synthesis. Although the role of TRAP in osteoblasts and osteocytes remains elusive, we speculate that the function is related to the capability of the enzyme to regulate the phosphorylation of proteins known to be expressed by these cells.</description><subject>Acid Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Bone Remodeling - physiology</subject><subject>Bones</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Disease Models, Animal</subject><subject>Endocrinology</subject><subject>Enzymes</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Isoenzymes - metabolism</subject><subject>Life Sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Microscopy, Electron, Transmission</subject><subject>Original Research</subject><subject>Orthopedics</subject><subject>Osteoblasts - enzymology</subject><subject>Osteoclasts - enzymology</subject><subject>Osteocytes - enzymology</subject><subject>Osteoporosis, Postmenopausal - enzymology</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rickets - enzymology</subject><subject>Rodents</subject><subject>Tartrate-Resistant Acid Phosphatase</subject><subject>Vitamin D</subject><issn>0171-967X</issn><issn>1432-0827</issn><issn>1432-0827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>D8T</sourceid><recordid>eNqNkl9r1TAYxoso7jj9AN5IwRtvonnzvzfCGFMHg8mY4F1I27c7nT1NTXKmwy9vas_mJgy8atrn9zzNmzxF8RLoW6BUv4uUMsYJBU4qwwXhj4oVCM4INUw_LlYUNJBK6a97xbMYLykFoZR6WuwxwSsJuloVv47HJqCL2JbnLqTgEpIzjH1MbkzlQdO35ee1j9PapQyVRz-ngDH2fiz7sTyNCX09uJhi6cZ2eW-uE8ZZzSyGfoNjcsMiTT74HD2LZy7F58WTzg0RX-ye-8WXD0fnh5_IyenH48ODE9IoqRNxknZGaceqtq6odkYBaCMROhAAUkFHa4RGmLbSTcWga8F0gLpGVRnZId8vyJIbf-C0re2Ud-XCtfWut7tP3_IKrWRCKpX56kF-Cr79a7oxAjPKAGc0e98v3gxssG3y9MEN9yPuKWO_thf-ygoQhnPIAW92AcF_32JMdtPHBofBjei30YIEpSVj6r9QIbikf0Z6_Q966bdhzKc-U8wwTsVMwUI1-Z5iwO5230Dt3Di7NM7mxtm5cZZnz6u7A986biqWAbY7zSyNFxju_PrB1N9oheSP</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Solberg, Lene B.</creator><creator>Brorson, Sverre-Henning</creator><creator>Stordalen, Gunhild A.</creator><creator>Bækkevold, Espen S.</creator><creator>Andersson, Göran</creator><creator>Reinholt, Finn P.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20140501</creationdate><title>Increased Tartrate-Resistant Acid Phosphatase Expression in Osteoblasts and Osteocytes in Experimental Osteoporosis in Rats</title><author>Solberg, Lene B. ; Brorson, Sverre-Henning ; Stordalen, Gunhild A. ; Bækkevold, Espen S. ; Andersson, Göran ; Reinholt, Finn P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c657t-a50f867a29db907a8611785e1f1411561f0be1c48d97c921fd18f1e7be6985fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acid Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Bone Remodeling - physiology</topic><topic>Bones</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Disease Models, Animal</topic><topic>Endocrinology</topic><topic>Enzymes</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Isoenzymes - metabolism</topic><topic>Life Sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Microscopy, Electron, Transmission</topic><topic>Original Research</topic><topic>Orthopedics</topic><topic>Osteoblasts - enzymology</topic><topic>Osteoclasts - enzymology</topic><topic>Osteocytes - enzymology</topic><topic>Osteoporosis, Postmenopausal - enzymology</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rickets - enzymology</topic><topic>Rodents</topic><topic>Tartrate-Resistant Acid Phosphatase</topic><topic>Vitamin D</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Solberg, Lene B.</creatorcontrib><creatorcontrib>Brorson, Sverre-Henning</creatorcontrib><creatorcontrib>Stordalen, Gunhild A.</creatorcontrib><creatorcontrib>Bækkevold, Espen S.</creatorcontrib><creatorcontrib>Andersson, Göran</creatorcontrib><creatorcontrib>Reinholt, Finn P.</creatorcontrib><collection>Springer_OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database (ProQuest)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Calcified tissue international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Solberg, Lene B.</au><au>Brorson, Sverre-Henning</au><au>Stordalen, Gunhild A.</au><au>Bækkevold, Espen S.</au><au>Andersson, Göran</au><au>Reinholt, Finn P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Tartrate-Resistant Acid Phosphatase Expression in Osteoblasts and Osteocytes in Experimental Osteoporosis in Rats</atitle><jtitle>Calcified tissue international</jtitle><stitle>Calcif Tissue Int</stitle><addtitle>Calcif Tissue Int</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>94</volume><issue>5</issue><spage>510</spage><epage>521</epage><pages>510-521</pages><issn>0171-967X</issn><issn>1432-0827</issn><eissn>1432-0827</eissn><abstract>Tartrate-resistant acid phosphatase (TRAP) is known as an osteoclast marker, but osteoblasts and osteocytes in the vicinity of bone remodeling sites also express TRAP. Cell culture studies suggest that osteoblasts endocytose osteoclastic TRAP for inactivation. To evaluate whether changes in osteoclast activity could alter TRAP expression in osteoblasts and/or osteocytes in vivo, we studied the ovariectomized and vitamin D-deficient rat (Ovx-D) and rats healing from rickets. Bone sections were analyzed for TRAP gene expression by in situ hybridization, TRAP protein by immunogold labeling, and TRAP enzyme activity using the fluorescent substrate ELF97. Osteoblasts and osteocytes close to intracortical remodeling sites and bone surfaces demonstrated TRAP, most prominently in cancellous bone and osteocytes. Intracellular TRAP was located to electron-dense vesicles with similar morphology in both cell types. Ovx-D increased osteoclast activity (
p
< 0.001) and ELF97
+
osteocytes (
p
< 0.05) in cancellous bone, but no corresponding increase was observed in the osteocyte lacunar area. The level of TRAP
+
vesicles in cortical osteoblasts (
p
< 0.01) in Ovx-D rats was also increased. Enhanced osteoclast activity was noted in healing rickets after 72 h (
p
< 0.05), but no differences in TRAP expression were detected in osteoblasts or osteocytes. Thus, increased osteoclast activity does not affect TRAP expression in osteoblasts and osteocytes, favoring the notion that increased TRAP in these cells is rather due to increased synthesis. Although the role of TRAP in osteoblasts and osteocytes remains elusive, we speculate that the function is related to the capability of the enzyme to regulate the phosphorylation of proteins known to be expressed by these cells.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24395179</pmid><doi>10.1007/s00223-013-9834-3</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Calcified tissue international, 2014-05, Vol.94 (5), p.510-521 |
| issn | 0171-967X 1432-0827 1432-0827 |
| language | eng |
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| source | MEDLINE; Springer Online Journals; SWEPUB Freely available online |
| subjects | Acid Phosphatase - metabolism Animals Biochemistry Biomedical and Life Sciences Bone Remodeling - physiology Bones Cell Biology Cell culture Disease Models, Animal Endocrinology Enzymes Female Fluorescent Antibody Technique Humans Immunohistochemistry In Situ Hybridization Isoenzymes - metabolism Life Sciences Medicin och hälsovetenskap Microscopy, Electron, Transmission Original Research Orthopedics Osteoblasts - enzymology Osteoclasts - enzymology Osteocytes - enzymology Osteoporosis, Postmenopausal - enzymology Proteins Rats Rickets - enzymology Rodents Tartrate-Resistant Acid Phosphatase Vitamin D |
| title | Increased Tartrate-Resistant Acid Phosphatase Expression in Osteoblasts and Osteocytes in Experimental Osteoporosis in Rats |
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