Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique
The diagnosis of neurocysticercosis (NCC) requires expensive neuroimaging techniques that are seldom affordable for people in endemic countries. Accordingly, there is a need for new low-cost diagnostic methods that offer high sensitivity and specificity. In this study, we evaluated Western blot anal...
Gespeichert in:
| Veröffentlicht in: | Journal of helminthology 2016-09, Vol.90 (5), p.630-633 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 633 |
|---|---|
| container_issue | 5 |
| container_start_page | 630 |
| container_title | Journal of helminthology |
| container_volume | 90 |
| creator | Nhancupe, N. Noormahomed, E.V. Afonso, S. Falk, K.I. Lindh, J. |
| description | The diagnosis of neurocysticercosis (NCC) requires expensive neuroimaging techniques that are seldom affordable for people in endemic countries. Accordingly, there is a need for new low-cost diagnostic methods that offer high sensitivity and specificity. In this study, we evaluated Western blot analysis of the previously described recombinant antigen Tsol-p27 in relation to a commercial or in-house enzyme-linked immunosorbent assay (ELISA) for NCC, and compared the results with those provided by a commercial enzyme-linked immunoelectrotransfer blot (EITB) assay, which was regarded as the reference standard method. The analysed serum samples were obtained from 165 people, 18 of whom were confirmed to be NCC positive by EITB. Comparing our Western blot analysis of Tsol-p27 with a previous evaluation performed in Central America showed similar specificity (96.69% versus 97.8%) and sensitivity (85.71% versus 86.7%). The present results indicate that the recombinant Tsol-p27 antigen provides good sensitivity and specificity, and might be preferable as a diagnostic antigen in poorly equipped laboratories in endemic countries. |
| doi_str_mv | 10.1017/S0022149X15000747 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_511254</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cupid>10_1017_S0022149X15000747</cupid><sourcerecordid>1877839990</sourcerecordid><originalsourceid>FETCH-LOGICAL-c579t-5d6b25860e299f2bc648bf9e7127acb10ae0bb75b3c29e46f3e8dc5f37c299f73</originalsourceid><addsrcrecordid>eNqNkl1rFTEQhoNY7Gn1B3gjC9540a352Gw2N0KpX4UWBat4IYQkO7tN3U2Oya5Sf71Zz7G0SkFykTDzvG9mkkHoMcGHBBPx_APGlJJKfiYcYywqcQ-tSCV4SWsp7qPVki6X_C7aS-kyM4xQ_gDt0ppKWuNmhb68h9iFOGpvoQhdcZ7CUK6pKLSfXA--yMliuoAiQQxD6J3VQ9E63fuQXFoU9ipNzkK0vwPOF2fhpx6N-zbDQ7TT6SHBo-2-jz6-fnV-_LY8fffm5PjotLRcyKnkbW0ob2oMVMqOGltXjekkCEKFtoZgDdgYwQ2zVEJVdwya1vKOCbsIBNtH5cY3_YD1bNQ6ulHHKxW0U9vQ13wCxUnuv8r8wZ38S_fpSIXYq3lWDOdFM_5ig2d2hNaCn6IebqluZ7y7UH34rmrWVBwv9T3bGsSQnyVNanTJwjBoD2FOijRCNExKif8DxYJnlMmMPv0LvQxz9PmhF0OCq2VlimwoG0NKEbrruglWyxSpf6Yoa57cbPha8WdsMsC2pvmno2t7uHH3nba_AFhE0ys</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1871040404</pqid></control><display><type>article</type><title>Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique</title><source>MEDLINE</source><source>SWEPUB Freely available online</source><source>Cambridge University Press Journals Complete</source><creator>Nhancupe, N. ; Noormahomed, E.V. ; Afonso, S. ; Falk, K.I. ; Lindh, J.</creator><creatorcontrib>Nhancupe, N. ; Noormahomed, E.V. ; Afonso, S. ; Falk, K.I. ; Lindh, J.</creatorcontrib><description>The diagnosis of neurocysticercosis (NCC) requires expensive neuroimaging techniques that are seldom affordable for people in endemic countries. Accordingly, there is a need for new low-cost diagnostic methods that offer high sensitivity and specificity. In this study, we evaluated Western blot analysis of the previously described recombinant antigen Tsol-p27 in relation to a commercial or in-house enzyme-linked immunosorbent assay (ELISA) for NCC, and compared the results with those provided by a commercial enzyme-linked immunoelectrotransfer blot (EITB) assay, which was regarded as the reference standard method. The analysed serum samples were obtained from 165 people, 18 of whom were confirmed to be NCC positive by EITB. Comparing our Western blot analysis of Tsol-p27 with a previous evaluation performed in Central America showed similar specificity (96.69% versus 97.8%) and sensitivity (85.71% versus 86.7%). The present results indicate that the recombinant Tsol-p27 antigen provides good sensitivity and specificity, and might be preferable as a diagnostic antigen in poorly equipped laboratories in endemic countries.</description><identifier>ISSN: 0022-149X</identifier><identifier>ISSN: 1475-2697</identifier><identifier>EISSN: 1475-2697</identifier><identifier>DOI: 10.1017/S0022149X15000747</identifier><identifier>PMID: 26292608</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Antibodies, Helminth - blood ; Antigens, Helminth - immunology ; Enzyme-Linked Immunosorbent Assay ; Immunoblotting ; Mozambique ; Neurocysticercosis - diagnosis ; Research Papers ; Sensitivity and Specificity ; Serologic Tests - methods ; Taenia</subject><ispartof>Journal of helminthology, 2016-09, Vol.90 (5), p.630-633</ispartof><rights>Copyright © Cambridge University Press 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c579t-5d6b25860e299f2bc648bf9e7127acb10ae0bb75b3c29e46f3e8dc5f37c299f73</citedby><cites>FETCH-LOGICAL-c579t-5d6b25860e299f2bc648bf9e7127acb10ae0bb75b3c29e46f3e8dc5f37c299f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0022149X15000747/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,230,314,551,777,781,882,27905,27906,55609</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26292608$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-303032$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:134025844$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Nhancupe, N.</creatorcontrib><creatorcontrib>Noormahomed, E.V.</creatorcontrib><creatorcontrib>Afonso, S.</creatorcontrib><creatorcontrib>Falk, K.I.</creatorcontrib><creatorcontrib>Lindh, J.</creatorcontrib><title>Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique</title><title>Journal of helminthology</title><addtitle>J. Helminthol</addtitle><description>The diagnosis of neurocysticercosis (NCC) requires expensive neuroimaging techniques that are seldom affordable for people in endemic countries. Accordingly, there is a need for new low-cost diagnostic methods that offer high sensitivity and specificity. In this study, we evaluated Western blot analysis of the previously described recombinant antigen Tsol-p27 in relation to a commercial or in-house enzyme-linked immunosorbent assay (ELISA) for NCC, and compared the results with those provided by a commercial enzyme-linked immunoelectrotransfer blot (EITB) assay, which was regarded as the reference standard method. The analysed serum samples were obtained from 165 people, 18 of whom were confirmed to be NCC positive by EITB. Comparing our Western blot analysis of Tsol-p27 with a previous evaluation performed in Central America showed similar specificity (96.69% versus 97.8%) and sensitivity (85.71% versus 86.7%). The present results indicate that the recombinant Tsol-p27 antigen provides good sensitivity and specificity, and might be preferable as a diagnostic antigen in poorly equipped laboratories in endemic countries.</description><subject>Antibodies, Helminth - blood</subject><subject>Antigens, Helminth - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Immunoblotting</subject><subject>Mozambique</subject><subject>Neurocysticercosis - diagnosis</subject><subject>Research Papers</subject><subject>Sensitivity and Specificity</subject><subject>Serologic Tests - methods</subject><subject>Taenia</subject><issn>0022-149X</issn><issn>1475-2697</issn><issn>1475-2697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>D8T</sourceid><recordid>eNqNkl1rFTEQhoNY7Gn1B3gjC9540a352Gw2N0KpX4UWBat4IYQkO7tN3U2Oya5Sf71Zz7G0SkFykTDzvG9mkkHoMcGHBBPx_APGlJJKfiYcYywqcQ-tSCV4SWsp7qPVki6X_C7aS-kyM4xQ_gDt0ppKWuNmhb68h9iFOGpvoQhdcZ7CUK6pKLSfXA--yMliuoAiQQxD6J3VQ9E63fuQXFoU9ipNzkK0vwPOF2fhpx6N-zbDQ7TT6SHBo-2-jz6-fnV-_LY8fffm5PjotLRcyKnkbW0ob2oMVMqOGltXjekkCEKFtoZgDdgYwQ2zVEJVdwya1vKOCbsIBNtH5cY3_YD1bNQ6ulHHKxW0U9vQ13wCxUnuv8r8wZ38S_fpSIXYq3lWDOdFM_5ig2d2hNaCn6IebqluZ7y7UH34rmrWVBwv9T3bGsSQnyVNanTJwjBoD2FOijRCNExKif8DxYJnlMmMPv0LvQxz9PmhF0OCq2VlimwoG0NKEbrruglWyxSpf6Yoa57cbPha8WdsMsC2pvmno2t7uHH3nba_AFhE0ys</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Nhancupe, N.</creator><creator>Noormahomed, E.V.</creator><creator>Afonso, S.</creator><creator>Falk, K.I.</creator><creator>Lindh, J.</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7SN</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DF2</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20160901</creationdate><title>Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique</title><author>Nhancupe, N. ; Noormahomed, E.V. ; Afonso, S. ; Falk, K.I. ; Lindh, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-5d6b25860e299f2bc648bf9e7127acb10ae0bb75b3c29e46f3e8dc5f37c299f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antibodies, Helminth - blood</topic><topic>Antigens, Helminth - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Immunoblotting</topic><topic>Mozambique</topic><topic>Neurocysticercosis - diagnosis</topic><topic>Research Papers</topic><topic>Sensitivity and Specificity</topic><topic>Serologic Tests - methods</topic><topic>Taenia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nhancupe, N.</creatorcontrib><creatorcontrib>Noormahomed, E.V.</creatorcontrib><creatorcontrib>Afonso, S.</creatorcontrib><creatorcontrib>Falk, K.I.</creatorcontrib><creatorcontrib>Lindh, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Uppsala universitet</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Journal of helminthology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nhancupe, N.</au><au>Noormahomed, E.V.</au><au>Afonso, S.</au><au>Falk, K.I.</au><au>Lindh, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique</atitle><jtitle>Journal of helminthology</jtitle><addtitle>J. Helminthol</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>90</volume><issue>5</issue><spage>630</spage><epage>633</epage><pages>630-633</pages><issn>0022-149X</issn><issn>1475-2697</issn><eissn>1475-2697</eissn><abstract>The diagnosis of neurocysticercosis (NCC) requires expensive neuroimaging techniques that are seldom affordable for people in endemic countries. Accordingly, there is a need for new low-cost diagnostic methods that offer high sensitivity and specificity. In this study, we evaluated Western blot analysis of the previously described recombinant antigen Tsol-p27 in relation to a commercial or in-house enzyme-linked immunosorbent assay (ELISA) for NCC, and compared the results with those provided by a commercial enzyme-linked immunoelectrotransfer blot (EITB) assay, which was regarded as the reference standard method. The analysed serum samples were obtained from 165 people, 18 of whom were confirmed to be NCC positive by EITB. Comparing our Western blot analysis of Tsol-p27 with a previous evaluation performed in Central America showed similar specificity (96.69% versus 97.8%) and sensitivity (85.71% versus 86.7%). The present results indicate that the recombinant Tsol-p27 antigen provides good sensitivity and specificity, and might be preferable as a diagnostic antigen in poorly equipped laboratories in endemic countries.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>26292608</pmid><doi>10.1017/S0022149X15000747</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-149X |
| ispartof | Journal of helminthology, 2016-09, Vol.90 (5), p.630-633 |
| issn | 0022-149X 1475-2697 1475-2697 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_511254 |
| source | MEDLINE; SWEPUB Freely available online; Cambridge University Press Journals Complete |
| subjects | Antibodies, Helminth - blood Antigens, Helminth - immunology Enzyme-Linked Immunosorbent Assay Immunoblotting Mozambique Neurocysticercosis - diagnosis Research Papers Sensitivity and Specificity Serologic Tests - methods Taenia |
| title | Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T14%3A23%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Performance%20of%20Tsol-p27%20antigen%20for%20the%20serological%20diagnosis%20of%20cysticercosis%20in%20Mozambique&rft.jtitle=Journal%20of%20helminthology&rft.au=Nhancupe,%20N.&rft.date=2016-09-01&rft.volume=90&rft.issue=5&rft.spage=630&rft.epage=633&rft.pages=630-633&rft.issn=0022-149X&rft.eissn=1475-2697&rft_id=info:doi/10.1017/S0022149X15000747&rft_dat=%3Cproquest_swepu%3E1877839990%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1871040404&rft_id=info:pmid/26292608&rft_cupid=10_1017_S0022149X15000747&rfr_iscdi=true |