Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015

In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription...

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Veröffentlicht in:	Euro surveillance : bulletin européen sur les maladies transmissibles 2015-11, Vol.20 (44), p.1
Hauptverfasser:	Faye, Oumar, Faye, Ousmane, Soropogui, Barré, Patel, Pranav, El Wahed, Ahmed Abd, Loucoubar, Cheikh, Fall, Gamou, Kiory, Davy, Magassouba, N'Faly, Keita, Sakoba, Kondé, Mandy Kader, Diallo, Alpha Amadou, Koivogui, Lamine, Karlberg, Helen, Mirazimi, Ali, Nentwich, Oliver, Piepenburg, Olaf, Niedrig, Matthias, Weidmann, Manfred, Sall, Amadou Alpha
Format:	Artikel
Sprache:	eng
Schlagworte:	Disease Outbreaks Early Diagnosis Ebola virus Ebolavirus - genetics Ebolavirus - isolation & purification Epidemics Guinea Hemorrhagic Fever, Ebola - diagnosis Hemorrhagic Fever, Ebola - virology Humans Polymerase chain reaction Real-Time Polymerase Chain Reaction Recombinases - metabolism Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis RNA, Viral - genetics Sensitivity and Specificity Surveillance Time Factors Vaccines
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