Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015
In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription...
Gespeichert in:
| Veröffentlicht in: | Euro surveillance : bulletin européen sur les maladies transmissibles 2015-11, Vol.20 (44), p.1 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 44 |
| container_start_page | 1 |
| container_title | Euro surveillance : bulletin européen sur les maladies transmissibles |
| container_volume | 20 |
| creator | Faye, Oumar Faye, Ousmane Soropogui, Barré Patel, Pranav El Wahed, Ahmed Abd Loucoubar, Cheikh Fall, Gamou Kiory, Davy Magassouba, N'Faly Keita, Sakoba Kondé, Mandy Kader Diallo, Alpha Amadou Koivogui, Lamine Karlberg, Helen Mirazimi, Ali Nentwich, Oliver Piepenburg, Olaf Niedrig, Matthias Weidmann, Manfred Sall, Amadou Alpha |
| description | In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription recombinase polymerase amplification assay for the detection of Ebola virus (EBOV-RT-RPA). The performance of combined extraction and detection was best for swabs. Sensitivity and specificity of the combined SE and EBOV-RT-RPA were tested in a mobile laboratory consisting of a mobile glovebox and a Diagnostics-in-a-Suitcase powered by a battery and solar panel, deployed to Matoto Conakry, Guinea as part of the reinforced surveillance strategy in April 2015 to reach the goal of zero cases. The EBOV-RT-RPA was evaluated in comparison to two real-time PCR assays. Of 928 post-mortem swabs, 120 tested positive, and the combined SE and EBOV-RT-RPA yielded a sensitivity and specificity of 100% in reference to one real-time RT-PCR assay. Another widely used real-time RT-PCR was much less sensitive than expected. Results were provided very fast within 30 to 60 min, and the field deployment of the mobile laboratory helped improve burial management and community engagement. |
| doi_str_mv | 10.2807/1560-7917.ES.2015.20.44.30053 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_509383</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1733188855</sourcerecordid><originalsourceid>FETCH-LOGICAL-c516t-80db0ef349ab431d9fd2f8ccaca491a9d98bd48651b47a5729506795062ea6f3</originalsourceid><addsrcrecordid>eNpdkU1v1DAQhi0EoqXwF5AlhMQlwY4_Yh84oLIUpEoc2rs1iR3JJYmDnbTsv8feXVaIy_j1-Jnx2C9C7ympG0Xaj1RIUrWatvXurm4IFTnUnNeMEMGeocvz-fN_9AV6ldIDIZwR3bxEF40UQklNLtHvL-7RjWGZ3LximC22bhnD_rANAwYcYfEWR9eHqfMzJIeXMObzWCRMy-gH38Pqw4x3XRgBP_q4pdxmdf0hCynBHvsZ32x-dlBUGfs1ejHAmNyb03qF7r_u7q-_Vbc_br5ff76tekHlWiliO-IGxjV0nFGrB9sMqu-hB64paKtVZ7mSgna8BdE2WhDZltA4kAO7QtWxbXpyy9aZJfoJ4t4E8OaU-pmVM4JopljmPxz5JYZfm0urmXzq3TjC7MKWDG0Zo0opITL67j_0IWxxzo8pVKNZK5XM1Kcj1ceQUnTDeQRKTHHUFJdMccns7kz5mhwM5-bgaK5_e7pl6yZnz9V_LWR_ADKtnhc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1732937686</pqid></control><display><type>article</type><title>Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>SWEPUB Freely available online</source><creator>Faye, Oumar ; Faye, Ousmane ; Soropogui, Barré ; Patel, Pranav ; El Wahed, Ahmed Abd ; Loucoubar, Cheikh ; Fall, Gamou ; Kiory, Davy ; Magassouba, N'Faly ; Keita, Sakoba ; Kondé, Mandy Kader ; Diallo, Alpha Amadou ; Koivogui, Lamine ; Karlberg, Helen ; Mirazimi, Ali ; Nentwich, Oliver ; Piepenburg, Olaf ; Niedrig, Matthias ; Weidmann, Manfred ; Sall, Amadou Alpha</creator><creatorcontrib>Faye, Oumar ; Faye, Ousmane ; Soropogui, Barré ; Patel, Pranav ; El Wahed, Ahmed Abd ; Loucoubar, Cheikh ; Fall, Gamou ; Kiory, Davy ; Magassouba, N'Faly ; Keita, Sakoba ; Kondé, Mandy Kader ; Diallo, Alpha Amadou ; Koivogui, Lamine ; Karlberg, Helen ; Mirazimi, Ali ; Nentwich, Oliver ; Piepenburg, Olaf ; Niedrig, Matthias ; Weidmann, Manfred ; Sall, Amadou Alpha</creatorcontrib><description>In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription recombinase polymerase amplification assay for the detection of Ebola virus (EBOV-RT-RPA). The performance of combined extraction and detection was best for swabs. Sensitivity and specificity of the combined SE and EBOV-RT-RPA were tested in a mobile laboratory consisting of a mobile glovebox and a Diagnostics-in-a-Suitcase powered by a battery and solar panel, deployed to Matoto Conakry, Guinea as part of the reinforced surveillance strategy in April 2015 to reach the goal of zero cases. The EBOV-RT-RPA was evaluated in comparison to two real-time PCR assays. Of 928 post-mortem swabs, 120 tested positive, and the combined SE and EBOV-RT-RPA yielded a sensitivity and specificity of 100% in reference to one real-time RT-PCR assay. Another widely used real-time RT-PCR was much less sensitive than expected. Results were provided very fast within 30 to 60 min, and the field deployment of the mobile laboratory helped improve burial management and community engagement.</description><identifier>ISSN: 1560-7917</identifier><identifier>ISSN: 1025-496X</identifier><identifier>EISSN: 1560-7917</identifier><identifier>DOI: 10.2807/1560-7917.ES.2015.20.44.30053</identifier><identifier>PMID: 26558690</identifier><language>eng</language><publisher>Sweden: Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS)</publisher><subject>Disease Outbreaks ; Early Diagnosis ; Ebola virus ; Ebolavirus - genetics ; Ebolavirus - isolation & purification ; Epidemics ; Guinea ; Hemorrhagic Fever, Ebola - diagnosis ; Hemorrhagic Fever, Ebola - virology ; Humans ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; Recombinases - metabolism ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Viral - analysis ; RNA, Viral - genetics ; Sensitivity and Specificity ; Surveillance ; Time Factors ; Vaccines</subject><ispartof>Euro surveillance : bulletin européen sur les maladies transmissibles, 2015-11, Vol.20 (44), p.1</ispartof><rights>Copyright Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS) Nov 5, 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-80db0ef349ab431d9fd2f8ccaca491a9d98bd48651b47a5729506795062ea6f3</citedby><cites>FETCH-LOGICAL-c516t-80db0ef349ab431d9fd2f8ccaca491a9d98bd48651b47a5729506795062ea6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,550,776,780,860,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26558690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:132379438$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Faye, Oumar</creatorcontrib><creatorcontrib>Faye, Ousmane</creatorcontrib><creatorcontrib>Soropogui, Barré</creatorcontrib><creatorcontrib>Patel, Pranav</creatorcontrib><creatorcontrib>El Wahed, Ahmed Abd</creatorcontrib><creatorcontrib>Loucoubar, Cheikh</creatorcontrib><creatorcontrib>Fall, Gamou</creatorcontrib><creatorcontrib>Kiory, Davy</creatorcontrib><creatorcontrib>Magassouba, N'Faly</creatorcontrib><creatorcontrib>Keita, Sakoba</creatorcontrib><creatorcontrib>Kondé, Mandy Kader</creatorcontrib><creatorcontrib>Diallo, Alpha Amadou</creatorcontrib><creatorcontrib>Koivogui, Lamine</creatorcontrib><creatorcontrib>Karlberg, Helen</creatorcontrib><creatorcontrib>Mirazimi, Ali</creatorcontrib><creatorcontrib>Nentwich, Oliver</creatorcontrib><creatorcontrib>Piepenburg, Olaf</creatorcontrib><creatorcontrib>Niedrig, Matthias</creatorcontrib><creatorcontrib>Weidmann, Manfred</creatorcontrib><creatorcontrib>Sall, Amadou Alpha</creatorcontrib><title>Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015</title><title>Euro surveillance : bulletin européen sur les maladies transmissibles</title><addtitle>Euro Surveill</addtitle><description>In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription recombinase polymerase amplification assay for the detection of Ebola virus (EBOV-RT-RPA). The performance of combined extraction and detection was best for swabs. Sensitivity and specificity of the combined SE and EBOV-RT-RPA were tested in a mobile laboratory consisting of a mobile glovebox and a Diagnostics-in-a-Suitcase powered by a battery and solar panel, deployed to Matoto Conakry, Guinea as part of the reinforced surveillance strategy in April 2015 to reach the goal of zero cases. The EBOV-RT-RPA was evaluated in comparison to two real-time PCR assays. Of 928 post-mortem swabs, 120 tested positive, and the combined SE and EBOV-RT-RPA yielded a sensitivity and specificity of 100% in reference to one real-time RT-PCR assay. Another widely used real-time RT-PCR was much less sensitive than expected. Results were provided very fast within 30 to 60 min, and the field deployment of the mobile laboratory helped improve burial management and community engagement.</description><subject>Disease Outbreaks</subject><subject>Early Diagnosis</subject><subject>Ebola virus</subject><subject>Ebolavirus - genetics</subject><subject>Ebolavirus - isolation & purification</subject><subject>Epidemics</subject><subject>Guinea</subject><subject>Hemorrhagic Fever, Ebola - diagnosis</subject><subject>Hemorrhagic Fever, Ebola - virology</subject><subject>Humans</subject><subject>Polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Recombinases - metabolism</subject><subject>Reproducibility of Results</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Surveillance</subject><subject>Time Factors</subject><subject>Vaccines</subject><issn>1560-7917</issn><issn>1025-496X</issn><issn>1560-7917</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNpdkU1v1DAQhi0EoqXwF5AlhMQlwY4_Yh84oLIUpEoc2rs1iR3JJYmDnbTsv8feXVaIy_j1-Jnx2C9C7ympG0Xaj1RIUrWatvXurm4IFTnUnNeMEMGeocvz-fN_9AV6ldIDIZwR3bxEF40UQklNLtHvL-7RjWGZ3LximC22bhnD_rANAwYcYfEWR9eHqfMzJIeXMObzWCRMy-gH38Pqw4x3XRgBP_q4pdxmdf0hCynBHvsZ32x-dlBUGfs1ejHAmNyb03qF7r_u7q-_Vbc_br5ff76tekHlWiliO-IGxjV0nFGrB9sMqu-hB64paKtVZ7mSgna8BdE2WhDZltA4kAO7QtWxbXpyy9aZJfoJ4t4E8OaU-pmVM4JopljmPxz5JYZfm0urmXzq3TjC7MKWDG0Zo0opITL67j_0IWxxzo8pVKNZK5XM1Kcj1ceQUnTDeQRKTHHUFJdMccns7kz5mhwM5-bgaK5_e7pl6yZnz9V_LWR_ADKtnhc</recordid><startdate>20151105</startdate><enddate>20151105</enddate><creator>Faye, Oumar</creator><creator>Faye, Ousmane</creator><creator>Soropogui, Barré</creator><creator>Patel, Pranav</creator><creator>El Wahed, Ahmed Abd</creator><creator>Loucoubar, Cheikh</creator><creator>Fall, Gamou</creator><creator>Kiory, Davy</creator><creator>Magassouba, N'Faly</creator><creator>Keita, Sakoba</creator><creator>Kondé, Mandy Kader</creator><creator>Diallo, Alpha Amadou</creator><creator>Koivogui, Lamine</creator><creator>Karlberg, Helen</creator><creator>Mirazimi, Ali</creator><creator>Nentwich, Oliver</creator><creator>Piepenburg, Olaf</creator><creator>Niedrig, Matthias</creator><creator>Weidmann, Manfred</creator><creator>Sall, Amadou Alpha</creator><general>Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20151105</creationdate><title>Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015</title><author>Faye, Oumar ; Faye, Ousmane ; Soropogui, Barré ; Patel, Pranav ; El Wahed, Ahmed Abd ; Loucoubar, Cheikh ; Fall, Gamou ; Kiory, Davy ; Magassouba, N'Faly ; Keita, Sakoba ; Kondé, Mandy Kader ; Diallo, Alpha Amadou ; Koivogui, Lamine ; Karlberg, Helen ; Mirazimi, Ali ; Nentwich, Oliver ; Piepenburg, Olaf ; Niedrig, Matthias ; Weidmann, Manfred ; Sall, Amadou Alpha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-80db0ef349ab431d9fd2f8ccaca491a9d98bd48651b47a5729506795062ea6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Disease Outbreaks</topic><topic>Early Diagnosis</topic><topic>Ebola virus</topic><topic>Ebolavirus - genetics</topic><topic>Ebolavirus - isolation & purification</topic><topic>Epidemics</topic><topic>Guinea</topic><topic>Hemorrhagic Fever, Ebola - diagnosis</topic><topic>Hemorrhagic Fever, Ebola - virology</topic><topic>Humans</topic><topic>Polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Recombinases - metabolism</topic><topic>Reproducibility of Results</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - genetics</topic><topic>Sensitivity and Specificity</topic><topic>Surveillance</topic><topic>Time Factors</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Faye, Oumar</creatorcontrib><creatorcontrib>Faye, Ousmane</creatorcontrib><creatorcontrib>Soropogui, Barré</creatorcontrib><creatorcontrib>Patel, Pranav</creatorcontrib><creatorcontrib>El Wahed, Ahmed Abd</creatorcontrib><creatorcontrib>Loucoubar, Cheikh</creatorcontrib><creatorcontrib>Fall, Gamou</creatorcontrib><creatorcontrib>Kiory, Davy</creatorcontrib><creatorcontrib>Magassouba, N'Faly</creatorcontrib><creatorcontrib>Keita, Sakoba</creatorcontrib><creatorcontrib>Kondé, Mandy Kader</creatorcontrib><creatorcontrib>Diallo, Alpha Amadou</creatorcontrib><creatorcontrib>Koivogui, Lamine</creatorcontrib><creatorcontrib>Karlberg, Helen</creatorcontrib><creatorcontrib>Mirazimi, Ali</creatorcontrib><creatorcontrib>Nentwich, Oliver</creatorcontrib><creatorcontrib>Piepenburg, Olaf</creatorcontrib><creatorcontrib>Niedrig, Matthias</creatorcontrib><creatorcontrib>Weidmann, Manfred</creatorcontrib><creatorcontrib>Sall, Amadou Alpha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Euro surveillance : bulletin européen sur les maladies transmissibles</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Faye, Oumar</au><au>Faye, Ousmane</au><au>Soropogui, Barré</au><au>Patel, Pranav</au><au>El Wahed, Ahmed Abd</au><au>Loucoubar, Cheikh</au><au>Fall, Gamou</au><au>Kiory, Davy</au><au>Magassouba, N'Faly</au><au>Keita, Sakoba</au><au>Kondé, Mandy Kader</au><au>Diallo, Alpha Amadou</au><au>Koivogui, Lamine</au><au>Karlberg, Helen</au><au>Mirazimi, Ali</au><au>Nentwich, Oliver</au><au>Piepenburg, Olaf</au><au>Niedrig, Matthias</au><au>Weidmann, Manfred</au><au>Sall, Amadou Alpha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015</atitle><jtitle>Euro surveillance : bulletin européen sur les maladies transmissibles</jtitle><addtitle>Euro Surveill</addtitle><date>2015-11-05</date><risdate>2015</risdate><volume>20</volume><issue>44</issue><spage>1</spage><pages>1-</pages><issn>1560-7917</issn><issn>1025-496X</issn><eissn>1560-7917</eissn><abstract>In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription recombinase polymerase amplification assay for the detection of Ebola virus (EBOV-RT-RPA). The performance of combined extraction and detection was best for swabs. Sensitivity and specificity of the combined SE and EBOV-RT-RPA were tested in a mobile laboratory consisting of a mobile glovebox and a Diagnostics-in-a-Suitcase powered by a battery and solar panel, deployed to Matoto Conakry, Guinea as part of the reinforced surveillance strategy in April 2015 to reach the goal of zero cases. The EBOV-RT-RPA was evaluated in comparison to two real-time PCR assays. Of 928 post-mortem swabs, 120 tested positive, and the combined SE and EBOV-RT-RPA yielded a sensitivity and specificity of 100% in reference to one real-time RT-PCR assay. Another widely used real-time RT-PCR was much less sensitive than expected. Results were provided very fast within 30 to 60 min, and the field deployment of the mobile laboratory helped improve burial management and community engagement.</abstract><cop>Sweden</cop><pub>Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS)</pub><pmid>26558690</pmid><doi>10.2807/1560-7917.ES.2015.20.44.30053</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1560-7917 |
| ispartof | Euro surveillance : bulletin européen sur les maladies transmissibles, 2015-11, Vol.20 (44), p.1 |
| issn | 1560-7917 1025-496X 1560-7917 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_509383 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; SWEPUB Freely available online |
| subjects | Disease Outbreaks Early Diagnosis Ebola virus Ebolavirus - genetics Ebolavirus - isolation & purification Epidemics Guinea Hemorrhagic Fever, Ebola - diagnosis Hemorrhagic Fever, Ebola - virology Humans Polymerase chain reaction Real-Time Polymerase Chain Reaction Recombinases - metabolism Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis RNA, Viral - genetics Sensitivity and Specificity Surveillance Time Factors Vaccines |
| title | Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T07%3A45%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20deployment%20of%20a%20rapid%20recombinase%20polymerase%20amplification%20Ebola%20virus%20detection%20assay%20in%20Guinea%20in%202015&rft.jtitle=Euro%20surveillance%20:%20bulletin%20europ%C3%A9en%20sur%20les%20maladies%20transmissibles&rft.au=Faye,%20Oumar&rft.date=2015-11-05&rft.volume=20&rft.issue=44&rft.spage=1&rft.pages=1-&rft.issn=1560-7917&rft.eissn=1560-7917&rft_id=info:doi/10.2807/1560-7917.ES.2015.20.44.30053&rft_dat=%3Cproquest_swepu%3E1733188855%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1732937686&rft_id=info:pmid/26558690&rfr_iscdi=true |