Genetic code expansion in stable cell lines enables encoded chromatin modification

Genetically encoded unnatural amino acids provide powerful strategies for modulating the molecular functions of proteins in mammalian cells. However, this approach has not been coupled to genome-wide measurements, because efficient incorporation of unnatural amino acids is limited to transient expre...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature methods 2016-02, Vol.13 (2), p.158-164
Hauptverfasser:	Elsässer, Simon J, Ernst, Russell J, Walker, Olivia S, Chin, Jason W
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/338/552 631/337/100 631/337/100/2285 631/92 82 82/80 Amino acids Amino Acids - metabolism Amino Acyl-tRNA Synthetases - genetics Amino Acyl-tRNA Synthetases - metabolism Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Cell Line Cellular biology Chromatin Chromatin - physiology Embryonic Stem Cells - metabolism Fibroblasts - metabolism Gene Expression Regulation, Enzymologic - physiology Genetic aspects Genetic Engineering Genetic research Genetic transcription Genetics HEK293 Cells Humans Life Sciences Mammals Medicin och hälsovetenskap Methanosarcina - enzymology Methanosarcina - metabolism Methanosarcina mazei Mice Properties Proteins Proteomics Stem cells
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!