Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins

We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations th...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of structural biology 2016-04, Vol.194 (1), p.1-7
Hauptverfasser:	Bokhove, Marcel, Sadat Al Hosseini, Hamed, Saito, Takako, Dioguardi, Elisa, Gegenschatz-Schmid, Katharina, Nishimura, Kaoru, Raj, Isha, de Sanctis, Daniele, Han, Ling, Jovine, Luca
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence CHO Cells Cricetinae Cricetulus Crystallization Crystallography, X-Ray Gene Expression Glycoproteins HEK293 Cells Humans Maltose-binding protein fusion Maltose-Binding Proteins - chemistry Maltose-Binding Proteins - genetics Maltose-Binding Proteins - metabolism Mammalian cell expression Medicin och hälsovetenskap Models, Molecular Molecular replacement Mutation Protein Conformation Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sf9 Cells Technical Note X-ray crystallography
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!