In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts
To elucidate processes in the osteoclastic bone resorption, visualise resorption and related actin reorganisation, a combination of imaging technologies and an applicable in vitro model is needed. Nanosized bone powder from matching species is deposited on any biocompatible surface in order to form...
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| Veröffentlicht in: | Scientific reports 2016-03, Vol.6 (1), p.22585-22585, Article 22585 |
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| creator | Deguchi, Takahiro Alanne, Maria H. Fazeli, Elnaz Fagerlund, Katja M. Pennanen, Paula Lehenkari, Petri Hänninen, Pekka E. Peltonen, Juha Näreoja, Tuomas |
| description | To elucidate processes in the osteoclastic bone resorption, visualise resorption and related actin reorganisation, a combination of imaging technologies and an applicable
in vitro
model is needed. Nanosized bone powder from matching species is deposited on any biocompatible surface in order to form a thin, translucent, smooth and elastic representation of injured bone. Osteoclasts cultured on the layer expressed matching morphology to ones cultured on sawed cortical bone slices. Resorption pits were easily identified by reflectance microscopy. The coating allowed actin structures on the bone interface to be visualised with super-resolution microscopy along with a detailed interlinked actin networks and actin branching in conjunction with V-ATPase, dynamin and Arp2/3 at actin patches. Furthermore, we measured the timescale of an adaptive osteoclast adhesion to bone by force spectroscopy experiments on live osteoclasts with bone-coated AFM cantilevers. Utilising the
in vitro
model and the advanced imaging technologies we localised immunofluorescence signals in respect to bone with high precision and detected resorption at its early stages. Put together, our data supports a cyclic model for resorption in human osteoclasts. |
| doi_str_mv | 10.1038/srep22585 |
| format | Article |
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in vitro
model is needed. Nanosized bone powder from matching species is deposited on any biocompatible surface in order to form a thin, translucent, smooth and elastic representation of injured bone. Osteoclasts cultured on the layer expressed matching morphology to ones cultured on sawed cortical bone slices. Resorption pits were easily identified by reflectance microscopy. The coating allowed actin structures on the bone interface to be visualised with super-resolution microscopy along with a detailed interlinked actin networks and actin branching in conjunction with V-ATPase, dynamin and Arp2/3 at actin patches. Furthermore, we measured the timescale of an adaptive osteoclast adhesion to bone by force spectroscopy experiments on live osteoclasts with bone-coated AFM cantilevers. Utilising the
in vitro
model and the advanced imaging technologies we localised immunofluorescence signals in respect to bone with high precision and detected resorption at its early stages. Put together, our data supports a cyclic model for resorption in human osteoclasts.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep22585</identifier><identifier>PMID: 26935172</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/106 ; 14/19 ; 14/3 ; 631/1647/245/2226 ; 631/57/2282 ; 631/80/128/1276 ; 631/80/79 ; 692/698/1671/63 ; Actin ; Adenosine triphosphatase ; Adhesion ; Atomic force microscopy ; Bone imaging ; Bone resorption ; Bone Resorption - metabolism ; Bone Resorption - pathology ; Cortical bone ; Dynamin ; Female ; H+-transporting ATPase ; Humanities and Social Sciences ; Humans ; Immunofluorescence ; Male ; Medicin och hälsovetenskap ; Microscopy ; Microscopy, Atomic Force ; Microscopy, Interference ; Models, Biological ; multidisciplinary ; Osteoclasts ; Osteoclasts - metabolism ; Osteoclasts - ultrastructure ; Powder ; Reflectance ; Science ; Spectroscopy</subject><ispartof>Scientific reports, 2016-03, Vol.6 (1), p.22585-22585, Article 22585</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Mar 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-7101d39768b55f8594910f4fac1d3b70a980dc96289250ce9a000081cbb145f93</citedby><cites>FETCH-LOGICAL-c526t-7101d39768b55f8594910f4fac1d3b70a980dc96289250ce9a000081cbb145f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776281/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776281/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26935172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:133106716$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Deguchi, Takahiro</creatorcontrib><creatorcontrib>Alanne, Maria H.</creatorcontrib><creatorcontrib>Fazeli, Elnaz</creatorcontrib><creatorcontrib>Fagerlund, Katja M.</creatorcontrib><creatorcontrib>Pennanen, Paula</creatorcontrib><creatorcontrib>Lehenkari, Petri</creatorcontrib><creatorcontrib>Hänninen, Pekka E.</creatorcontrib><creatorcontrib>Peltonen, Juha</creatorcontrib><creatorcontrib>Näreoja, Tuomas</creatorcontrib><title>In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>To elucidate processes in the osteoclastic bone resorption, visualise resorption and related actin reorganisation, a combination of imaging technologies and an applicable
in vitro
model is needed. Nanosized bone powder from matching species is deposited on any biocompatible surface in order to form a thin, translucent, smooth and elastic representation of injured bone. Osteoclasts cultured on the layer expressed matching morphology to ones cultured on sawed cortical bone slices. Resorption pits were easily identified by reflectance microscopy. The coating allowed actin structures on the bone interface to be visualised with super-resolution microscopy along with a detailed interlinked actin networks and actin branching in conjunction with V-ATPase, dynamin and Arp2/3 at actin patches. Furthermore, we measured the timescale of an adaptive osteoclast adhesion to bone by force spectroscopy experiments on live osteoclasts with bone-coated AFM cantilevers. Utilising the
in vitro
model and the advanced imaging technologies we localised immunofluorescence signals in respect to bone with high precision and detected resorption at its early stages. Put together, our data supports a cyclic model for resorption in human osteoclasts.</description><subject>13/106</subject><subject>14/19</subject><subject>14/3</subject><subject>631/1647/245/2226</subject><subject>631/57/2282</subject><subject>631/80/128/1276</subject><subject>631/80/79</subject><subject>692/698/1671/63</subject><subject>Actin</subject><subject>Adenosine triphosphatase</subject><subject>Adhesion</subject><subject>Atomic force microscopy</subject><subject>Bone imaging</subject><subject>Bone resorption</subject><subject>Bone Resorption - metabolism</subject><subject>Bone Resorption - pathology</subject><subject>Cortical bone</subject><subject>Dynamin</subject><subject>Female</subject><subject>H+-transporting ATPase</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Male</subject><subject>Medicin och hälsovetenskap</subject><subject>Microscopy</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Interference</subject><subject>Models, Biological</subject><subject>multidisciplinary</subject><subject>Osteoclasts</subject><subject>Osteoclasts - metabolism</subject><subject>Osteoclasts - ultrastructure</subject><subject>Powder</subject><subject>Reflectance</subject><subject>Science</subject><subject>Spectroscopy</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>D8T</sourceid><recordid>eNp1kk1v1DAQhi0EotXSA38AWeJCkQK2E8f2Bamq-KhUiQucLceZbF0SO9hOEf--jna7bEH4Ymvmmdczoxehl5S8o6SW71OEmTEu-RN0ykjDK1Yz9vTofYLOUrol5XCmGqqeoxPWqppTwU5RuPL4zuUY8BR6GHEYcBc84BzwYKwbXTYZ8AQmLREm8HklTH8DyQWPhxAtJGx8j9MyQ6wipDAuec25yWyd3658SBmCHU3K6QV6Npgxwdn-3qDvnz5-u_xSXX_9fHV5cV1ZztpcCUpoXyvRyo7zQXLVKEqGprRUwp0gRknSW9UyqRgnFpRZ55PUdh1t-KDqDap2uukXzEun51j6ib91ME7vQz_KCzQnreR14dV_-TmG_k_RQyGta0paQdtS-2FXW4AJelu2FM34WOJRxrsbvQ13uhGiTECLwJu9QAw_F0hZTy5ZGEfjISxJUyGIbCnloqCv_0JvwxJ9WaWmUsnCkTLOBp3vKBtDKgYZDs1QolfX6INrCvvquPsD-eCRArzdr6ak_Bbi0Zf_qN0DXtHONg</recordid><startdate>20160303</startdate><enddate>20160303</enddate><creator>Deguchi, Takahiro</creator><creator>Alanne, Maria H.</creator><creator>Fazeli, Elnaz</creator><creator>Fagerlund, Katja M.</creator><creator>Pennanen, Paula</creator><creator>Lehenkari, Petri</creator><creator>Hänninen, Pekka E.</creator><creator>Peltonen, Juha</creator><creator>Näreoja, Tuomas</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20160303</creationdate><title>In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts</title><author>Deguchi, Takahiro ; Alanne, Maria H. ; Fazeli, Elnaz ; Fagerlund, Katja M. ; Pennanen, Paula ; Lehenkari, Petri ; Hänninen, Pekka E. ; Peltonen, Juha ; Näreoja, Tuomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-7101d39768b55f8594910f4fac1d3b70a980dc96289250ce9a000081cbb145f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13/106</topic><topic>14/19</topic><topic>14/3</topic><topic>631/1647/245/2226</topic><topic>631/57/2282</topic><topic>631/80/128/1276</topic><topic>631/80/79</topic><topic>692/698/1671/63</topic><topic>Actin</topic><topic>Adenosine triphosphatase</topic><topic>Adhesion</topic><topic>Atomic force microscopy</topic><topic>Bone imaging</topic><topic>Bone resorption</topic><topic>Bone Resorption - metabolism</topic><topic>Bone Resorption - pathology</topic><topic>Cortical bone</topic><topic>Dynamin</topic><topic>Female</topic><topic>H+-transporting ATPase</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Male</topic><topic>Medicin och hälsovetenskap</topic><topic>Microscopy</topic><topic>Microscopy, Atomic Force</topic><topic>Microscopy, Interference</topic><topic>Models, Biological</topic><topic>multidisciplinary</topic><topic>Osteoclasts</topic><topic>Osteoclasts - metabolism</topic><topic>Osteoclasts - ultrastructure</topic><topic>Powder</topic><topic>Reflectance</topic><topic>Science</topic><topic>Spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deguchi, Takahiro</creatorcontrib><creatorcontrib>Alanne, Maria H.</creatorcontrib><creatorcontrib>Fazeli, Elnaz</creatorcontrib><creatorcontrib>Fagerlund, Katja M.</creatorcontrib><creatorcontrib>Pennanen, Paula</creatorcontrib><creatorcontrib>Lehenkari, Petri</creatorcontrib><creatorcontrib>Hänninen, Pekka E.</creatorcontrib><creatorcontrib>Peltonen, Juha</creatorcontrib><creatorcontrib>Näreoja, Tuomas</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deguchi, Takahiro</au><au>Alanne, Maria H.</au><au>Fazeli, Elnaz</au><au>Fagerlund, Katja M.</au><au>Pennanen, Paula</au><au>Lehenkari, Petri</au><au>Hänninen, Pekka E.</au><au>Peltonen, Juha</au><au>Näreoja, Tuomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-03-03</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>22585</spage><epage>22585</epage><pages>22585-22585</pages><artnum>22585</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To elucidate processes in the osteoclastic bone resorption, visualise resorption and related actin reorganisation, a combination of imaging technologies and an applicable
in vitro
model is needed. Nanosized bone powder from matching species is deposited on any biocompatible surface in order to form a thin, translucent, smooth and elastic representation of injured bone. Osteoclasts cultured on the layer expressed matching morphology to ones cultured on sawed cortical bone slices. Resorption pits were easily identified by reflectance microscopy. The coating allowed actin structures on the bone interface to be visualised with super-resolution microscopy along with a detailed interlinked actin networks and actin branching in conjunction with V-ATPase, dynamin and Arp2/3 at actin patches. Furthermore, we measured the timescale of an adaptive osteoclast adhesion to bone by force spectroscopy experiments on live osteoclasts with bone-coated AFM cantilevers. Utilising the
in vitro
model and the advanced imaging technologies we localised immunofluorescence signals in respect to bone with high precision and detected resorption at its early stages. Put together, our data supports a cyclic model for resorption in human osteoclasts.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26935172</pmid><doi>10.1038/srep22585</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/106 14/19 14/3 631/1647/245/2226 631/57/2282 631/80/128/1276 631/80/79 692/698/1671/63 Actin Adenosine triphosphatase Adhesion Atomic force microscopy Bone imaging Bone resorption Bone Resorption - metabolism Bone Resorption - pathology Cortical bone Dynamin Female H+-transporting ATPase Humanities and Social Sciences Humans Immunofluorescence Male Medicin och hälsovetenskap Microscopy Microscopy, Atomic Force Microscopy, Interference Models, Biological multidisciplinary Osteoclasts Osteoclasts - metabolism Osteoclasts - ultrastructure Powder Reflectance Science Spectroscopy |
| title | In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts |
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