Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus
The human leukemia cell line HL-60 is considered an alternative cell culture model to study neutrophil differentiation and migration. The aim of this study was to characterize the suitability of HL-60 cells differentiated to neutrophil-like cells (nHL-60) as substitute for blood-derived human neutro...
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| Veröffentlicht in: | JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE 2017-02, Vol.16 (1), p.2-2, Article 2 |
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| container_title | JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE |
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| creator | Yaseen, Ragheda Blodkamp, Stefanie Lüthje, Petra Reuner, Friederike Völlger, Lena Naim, Hassan Y von Köckritz-Blickwede, Maren |
| description | The human leukemia cell line HL-60 is considered an alternative cell culture model to study neutrophil differentiation and migration. The aim of this study was to characterize the suitability of HL-60 cells differentiated to neutrophil-like cells (nHL-60) as substitute for blood-derived human neutrophils to investigate the interaction of neutrophils with Staphylococcus aureus.
For this purpose, antimicrobial activity, bacterial uptake, production of reactive oxygen species and the release of neutrophil extracellular traps (NETs) by nHL-60 cells were analyzed and compared to primary blood-derived neutrophils using Staphylococcus aureus as important human and animal pathogen.
Overall, the antimicrobial activities of nHL-60 cells were distinctly lower compared to blood-derived neutrophils. Furthermore, production of reactive oxygen species as well as NET formation was clearly impaired in nHL-60 cells.
This study indicates that HL-60 cells are of limited usage as an alternative model to study antimicrobial functions of neutrophils against Staphylococcus aureus. |
| doi_str_mv | 10.1186/s12952-017-0067-2 |
| format | Article |
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For this purpose, antimicrobial activity, bacterial uptake, production of reactive oxygen species and the release of neutrophil extracellular traps (NETs) by nHL-60 cells were analyzed and compared to primary blood-derived neutrophils using Staphylococcus aureus as important human and animal pathogen.
Overall, the antimicrobial activities of nHL-60 cells were distinctly lower compared to blood-derived neutrophils. Furthermore, production of reactive oxygen species as well as NET formation was clearly impaired in nHL-60 cells.
This study indicates that HL-60 cells are of limited usage as an alternative model to study antimicrobial functions of neutrophils against Staphylococcus aureus.</description><identifier>ISSN: 1477-5751</identifier><identifier>EISSN: 1477-5751</identifier><identifier>DOI: 10.1186/s12952-017-0067-2</identifier><identifier>PMID: 28214466</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Anti-Infective Agents - metabolism ; Brief Report ; Cells, Cultured ; Extracellular Traps - drug effects ; Extracellular Traps - metabolism ; Fluorescence ; Health aspects ; HL-60 Cells ; Humans ; Leukemia ; Neutrophils ; Neutrophils - drug effects ; Neutrophils - immunology ; Reactive Oxygen Species - metabolism ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - physiology ; Tetradecanoylphorbol Acetate - pharmacology</subject><ispartof>JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE, 2017-02, Vol.16 (1), p.2-2, Article 2</ispartof><rights>COPYRIGHT 2017 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2017</rights><rights>The Author(s). 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-c86bba21359052a29060e4962d21896e99e84b1f73bce9804f564ea55a211c5e3</citedby><cites>FETCH-LOGICAL-c565t-c86bba21359052a29060e4962d21896e99e84b1f73bce9804f564ea55a211c5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316427/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316427/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,550,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28214466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:228214466$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Yaseen, Ragheda</creatorcontrib><creatorcontrib>Blodkamp, Stefanie</creatorcontrib><creatorcontrib>Lüthje, Petra</creatorcontrib><creatorcontrib>Reuner, Friederike</creatorcontrib><creatorcontrib>Völlger, Lena</creatorcontrib><creatorcontrib>Naim, Hassan Y</creatorcontrib><creatorcontrib>von Köckritz-Blickwede, Maren</creatorcontrib><title>Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus</title><title>JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE</title><addtitle>J Negat Results Biomed</addtitle><description>The human leukemia cell line HL-60 is considered an alternative cell culture model to study neutrophil differentiation and migration. The aim of this study was to characterize the suitability of HL-60 cells differentiated to neutrophil-like cells (nHL-60) as substitute for blood-derived human neutrophils to investigate the interaction of neutrophils with Staphylococcus aureus.
For this purpose, antimicrobial activity, bacterial uptake, production of reactive oxygen species and the release of neutrophil extracellular traps (NETs) by nHL-60 cells were analyzed and compared to primary blood-derived neutrophils using Staphylococcus aureus as important human and animal pathogen.
Overall, the antimicrobial activities of nHL-60 cells were distinctly lower compared to blood-derived neutrophils. Furthermore, production of reactive oxygen species as well as NET formation was clearly impaired in nHL-60 cells.
This study indicates that HL-60 cells are of limited usage as an alternative model to study antimicrobial functions of neutrophils against Staphylococcus aureus.</description><subject>Anti-Infective Agents - metabolism</subject><subject>Brief Report</subject><subject>Cells, Cultured</subject><subject>Extracellular Traps - drug effects</subject><subject>Extracellular Traps - metabolism</subject><subject>Fluorescence</subject><subject>Health aspects</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Neutrophils</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - immunology</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><issn>1477-5751</issn><issn>1477-5751</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>D8T</sourceid><recordid>eNqNkk2P0zAQhiMEYj_gB3BBkbhwyeJx_HlBqlawi1SJA3C2HGfSeknjrp101X-Po5ZlizggH2zNPO_r0egtijdArgCU-JCAak4rArIiRMiKPivOgUlZccnh-ZP3WXGR0h0hlEghXxZnVFFgTIjz4n4xjH7jXQyNt31p3eh3ftyXoStvl5UgpcO-T6ULm62N2JZjKLfRb2zcl00fQlu1GP0uNwacxhi2a59pu7J-SGP5bbTb9b4PLjg35fIUcUqvihed7RO-Pt6XxY_Pn75f31bLrzdfrhfLynHBx8op0TSWQs014dRSTQRBpgVtKSgtUGtUrIFO1o1DrQjruGBoOc8acBzry6I6-KYH3E6NOY5tgvXmWPqZX2iYVlqqzH888LmzwdbhMEbbn8hOO4Nfm1XYGV6DYFRmg_dHgxjuJ0yj2fg0r88OGKZkQEmpgFAN_4OS7MkUyei7v9C7MMUhb26mGCU1Af6HWtkejR-6kEd0s6lZMAUSGGfzt1f_oPJpMUcgDNj5XD8RwEGQ85FSxO5xHUDMHEFziKDJETRzBA3NmrdP9_io-J25-hc6aNbU</recordid><startdate>20170219</startdate><enddate>20170219</enddate><creator>Yaseen, Ragheda</creator><creator>Blodkamp, Stefanie</creator><creator>Lüthje, Petra</creator><creator>Reuner, Friederike</creator><creator>Völlger, Lena</creator><creator>Naim, Hassan Y</creator><creator>von Köckritz-Blickwede, Maren</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20170219</creationdate><title>Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus</title><author>Yaseen, Ragheda ; Blodkamp, Stefanie ; Lüthje, Petra ; Reuner, Friederike ; Völlger, Lena ; Naim, Hassan Y ; von Köckritz-Blickwede, Maren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-c86bba21359052a29060e4962d21896e99e84b1f73bce9804f564ea55a211c5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Anti-Infective Agents - 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The aim of this study was to characterize the suitability of HL-60 cells differentiated to neutrophil-like cells (nHL-60) as substitute for blood-derived human neutrophils to investigate the interaction of neutrophils with Staphylococcus aureus.
For this purpose, antimicrobial activity, bacterial uptake, production of reactive oxygen species and the release of neutrophil extracellular traps (NETs) by nHL-60 cells were analyzed and compared to primary blood-derived neutrophils using Staphylococcus aureus as important human and animal pathogen.
Overall, the antimicrobial activities of nHL-60 cells were distinctly lower compared to blood-derived neutrophils. Furthermore, production of reactive oxygen species as well as NET formation was clearly impaired in nHL-60 cells.
This study indicates that HL-60 cells are of limited usage as an alternative model to study antimicrobial functions of neutrophils against Staphylococcus aureus.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>28214466</pmid><doi>10.1186/s12952-017-0067-2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; BioMedCentral; EZB-FREE-00999 freely available EZB journals; PubMed Central; SWEPUB Freely available online; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Anti-Infective Agents - metabolism Brief Report Cells, Cultured Extracellular Traps - drug effects Extracellular Traps - metabolism Fluorescence Health aspects HL-60 Cells Humans Leukemia Neutrophils Neutrophils - drug effects Neutrophils - immunology Reactive Oxygen Species - metabolism Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Tetradecanoylphorbol Acetate - pharmacology |
| title | Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus |
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