Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats
Objective Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese fem...
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| Veröffentlicht in: | Obesity (Silver Spring, Md.) Md.), 2018-07, Vol.26 (7), p.1161-1167 |
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| container_title | Obesity (Silver Spring, Md.) |
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| creator | Wagner, Isabel Viola Sahlin, Lena Savchuk, Iuliia Klöting, Nora Svechnikov, Konstantin Söder, Olle |
| description | Objective
Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats.
Methods
Gene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes. The conversion of pregnenolone to androgens was analyzed by thin‐layer chromatography.
Results
Substantial amounts of testosterone in adipose tissue (25‐153 ng/g tissue) and in the supernatant of adipocytes (0.33‐0.69 ng/ten thousand cells]) were found. StAR and steroidogenic enzymes encoded by genes including Cyp11A1, Cyp17A1, Cyp19, Hsd3b2, Hsd17b3, and Srd5a2 were expressed in adipose tissue and cultured cells. Thin layer chromatography data revealed that preadipocytes and adipocytes were able to convert pregnenolone to testosterone. Higher levels for all steroidogenic enzymes were found in both depots of obese animals compared with lean animals, with significantly higher levels in inguinal tissue.
Conclusions
The whole steroidogenic machinery and capacity for testosterone biosynthesis were found in fat depots of female rats. These findings support the hypothesis that adipose tissue may contribute substantially to the hyperandrogenism in female obesity. |
| doi_str_mv | 10.1002/oby.22198 |
| format | Article |
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Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats.
Methods
Gene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes. The conversion of pregnenolone to androgens was analyzed by thin‐layer chromatography.
Results
Substantial amounts of testosterone in adipose tissue (25‐153 ng/g tissue) and in the supernatant of adipocytes (0.33‐0.69 ng/ten thousand cells]) were found. StAR and steroidogenic enzymes encoded by genes including Cyp11A1, Cyp17A1, Cyp19, Hsd3b2, Hsd17b3, and Srd5a2 were expressed in adipose tissue and cultured cells. Thin layer chromatography data revealed that preadipocytes and adipocytes were able to convert pregnenolone to testosterone. Higher levels for all steroidogenic enzymes were found in both depots of obese animals compared with lean animals, with significantly higher levels in inguinal tissue.
Conclusions
The whole steroidogenic machinery and capacity for testosterone biosynthesis were found in fat depots of female rats. These findings support the hypothesis that adipose tissue may contribute substantially to the hyperandrogenism in female obesity.</description><identifier>ISSN: 1930-7381</identifier><identifier>ISSN: 1930-739X</identifier><identifier>EISSN: 1930-739X</identifier><identifier>DOI: 10.1002/oby.22198</identifier><identifier>PMID: 29901265</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Body fat ; Insulin resistance ; Laboratories ; Medicin och hälsovetenskap ; Obesity ; Polycystic ovary syndrome ; Rodents ; Studies ; Systematic review</subject><ispartof>Obesity (Silver Spring, Md.), 2018-07, Vol.26 (7), p.1161-1167</ispartof><rights>2018 The Obesity Society</rights><rights>2018 The Obesity Society.</rights><rights>Copyright Blackwell Publishing Ltd. Jul 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4768-42074dc9f8466732fc7812be697601366f0fcbd6dca31561f456038fe0ce9a913</citedby><cites>FETCH-LOGICAL-c4768-42074dc9f8466732fc7812be697601366f0fcbd6dca31561f456038fe0ce9a913</cites><orcidid>0000-0001-8764-149X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Foby.22198$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Foby.22198$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,1434,27928,27929,45578,45579,46413,46837</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29901265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:138577106$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Wagner, Isabel Viola</creatorcontrib><creatorcontrib>Sahlin, Lena</creatorcontrib><creatorcontrib>Savchuk, Iuliia</creatorcontrib><creatorcontrib>Klöting, Nora</creatorcontrib><creatorcontrib>Svechnikov, Konstantin</creatorcontrib><creatorcontrib>Söder, Olle</creatorcontrib><title>Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats</title><title>Obesity (Silver Spring, Md.)</title><addtitle>Obesity (Silver Spring)</addtitle><description>Objective
Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats.
Methods
Gene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes. The conversion of pregnenolone to androgens was analyzed by thin‐layer chromatography.
Results
Substantial amounts of testosterone in adipose tissue (25‐153 ng/g tissue) and in the supernatant of adipocytes (0.33‐0.69 ng/ten thousand cells]) were found. StAR and steroidogenic enzymes encoded by genes including Cyp11A1, Cyp17A1, Cyp19, Hsd3b2, Hsd17b3, and Srd5a2 were expressed in adipose tissue and cultured cells. Thin layer chromatography data revealed that preadipocytes and adipocytes were able to convert pregnenolone to testosterone. Higher levels for all steroidogenic enzymes were found in both depots of obese animals compared with lean animals, with significantly higher levels in inguinal tissue.
Conclusions
The whole steroidogenic machinery and capacity for testosterone biosynthesis were found in fat depots of female rats. These findings support the hypothesis that adipose tissue may contribute substantially to the hyperandrogenism in female obesity.</description><subject>Body fat</subject><subject>Insulin resistance</subject><subject>Laboratories</subject><subject>Medicin och hälsovetenskap</subject><subject>Obesity</subject><subject>Polycystic ovary syndrome</subject><subject>Rodents</subject><subject>Studies</subject><subject>Systematic review</subject><issn>1930-7381</issn><issn>1930-739X</issn><issn>1930-739X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kV1LHTEQhoO0qLW98A9IoDf2YjVfm2wurfhRsJxSFepVyGYnEt3dbDdnkfPvjd3jEQpezTA87zvDvAjtU3JECWHHsV4dMUZ1tYV2qeakUFz_-bDpK7qDPqX0QIiQpKTbaIdpTSiT5S76edKEISbANyGlCXBI2OJfcQn9MtgWX8dpdICjx5erAUbbN2O8hz6kDoceL2rIynPobAv4t12mz-ijt22CL-u6h27Pz25OL4urxcWP05Orwgklq0IwokTjtK-ElIoz71RFWQ1SK0kol9IT7-pGNs5yWkrqRSkJrzwQB9pqyvdQMfumJxim2gxj6Oy4MtEGsx495g6MqFRekHn9Lj-MsXkTvQopr0qlKJFZezhrM_h3grQ0XUgO2tb2EKdkGCnziZyxF_Trf-hD_l-fP5EpyaRQlIpMfZspN8aURvCbcygxL3manKf5l2dmD9aOU91BsyFfA8zA8Qw8hRZW7zuZxfe72fIZapGo7g</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Wagner, Isabel Viola</creator><creator>Sahlin, Lena</creator><creator>Savchuk, Iuliia</creator><creator>Klöting, Nora</creator><creator>Svechnikov, Konstantin</creator><creator>Söder, Olle</creator><general>Blackwell Publishing Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><orcidid>https://orcid.org/0000-0001-8764-149X</orcidid></search><sort><creationdate>201807</creationdate><title>Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats</title><author>Wagner, Isabel Viola ; Sahlin, Lena ; Savchuk, Iuliia ; Klöting, Nora ; Svechnikov, Konstantin ; Söder, Olle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4768-42074dc9f8466732fc7812be697601366f0fcbd6dca31561f456038fe0ce9a913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Body fat</topic><topic>Insulin resistance</topic><topic>Laboratories</topic><topic>Medicin och hälsovetenskap</topic><topic>Obesity</topic><topic>Polycystic ovary syndrome</topic><topic>Rodents</topic><topic>Studies</topic><topic>Systematic review</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wagner, Isabel Viola</creatorcontrib><creatorcontrib>Sahlin, Lena</creatorcontrib><creatorcontrib>Savchuk, Iuliia</creatorcontrib><creatorcontrib>Klöting, Nora</creatorcontrib><creatorcontrib>Svechnikov, Konstantin</creatorcontrib><creatorcontrib>Söder, Olle</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Obesity (Silver Spring, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wagner, Isabel Viola</au><au>Sahlin, Lena</au><au>Savchuk, Iuliia</au><au>Klöting, Nora</au><au>Svechnikov, Konstantin</au><au>Söder, Olle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats</atitle><jtitle>Obesity (Silver Spring, Md.)</jtitle><addtitle>Obesity (Silver Spring)</addtitle><date>2018-07</date><risdate>2018</risdate><volume>26</volume><issue>7</issue><spage>1161</spage><epage>1167</epage><pages>1161-1167</pages><issn>1930-7381</issn><issn>1930-739X</issn><eissn>1930-739X</eissn><abstract>Objective
Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats.
Methods
Gene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes. The conversion of pregnenolone to androgens was analyzed by thin‐layer chromatography.
Results
Substantial amounts of testosterone in adipose tissue (25‐153 ng/g tissue) and in the supernatant of adipocytes (0.33‐0.69 ng/ten thousand cells]) were found. StAR and steroidogenic enzymes encoded by genes including Cyp11A1, Cyp17A1, Cyp19, Hsd3b2, Hsd17b3, and Srd5a2 were expressed in adipose tissue and cultured cells. Thin layer chromatography data revealed that preadipocytes and adipocytes were able to convert pregnenolone to testosterone. Higher levels for all steroidogenic enzymes were found in both depots of obese animals compared with lean animals, with significantly higher levels in inguinal tissue.
Conclusions
The whole steroidogenic machinery and capacity for testosterone biosynthesis were found in fat depots of female rats. These findings support the hypothesis that adipose tissue may contribute substantially to the hyperandrogenism in female obesity.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>29901265</pmid><doi>10.1002/oby.22198</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-8764-149X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Body fat Insulin resistance Laboratories Medicin och hälsovetenskap Obesity Polycystic ovary syndrome Rodents Studies Systematic review |
| title | Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats |
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