Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency
Problem Cervical insufficiency is a precursor of preterm birth. Treatment with emergency cervical cerclage is contraindicated in the presence of intra‐amniotic infection. Detecting infection with Gram stain and culture of amniotic fluid lacks sensitivity. Proteomic profiling of amniotic fluid in cer...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2018-11, Vol.80 (5), p.e13030-n/a |
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| container_title | American journal of reproductive immunology (1989) |
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| creator | Govia, Rachelle N. M. Birse, Kenzie D. Sepehri, Shadi Khafipour, Ehsan Menticoglou, Savas M. Burgener, Adam D. Poliquin, Vanessa |
| description | Problem
Cervical insufficiency is a precursor of preterm birth. Treatment with emergency cervical cerclage is contraindicated in the presence of intra‐amniotic infection. Detecting infection with Gram stain and culture of amniotic fluid lacks sensitivity. Proteomic profiling of amniotic fluid in cervical insufficiency may help identify pregnancies best suited for emergency cerclage.
Method of study
Thirty‐two pregnant women underwent amniocentesis for routine genetic testing (n = 22) or after diagnosis of cervical insufficiency (n = 10). The proteomic profiles of the amniotic fluid samples were compared in a cross‐sectional fashion, including sub‐analyses of women with cervical insufficiency and latency periods of 1 week post‐diagnosis.
Results
Mean gestational age at diagnosis of cervical insufficiency was 21.4 weeks (95% CI 20.6‐22.1). Proteomic analysis yielded 40 (7.2%, P |
| doi_str_mv | 10.1111/aji.13030 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_486504</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2124710956</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4410-a5155ee75b29883f786839c469f7306390eff0d31872e8abee847cf045fc01a63</originalsourceid><addsrcrecordid>eNp1kUFv1DAQhS0EoqVw4A8gS1zoIe04dmLnuKqgFFXiAmfL64xbL0m8xElX---ZJdsiIXUuY4--9zSax9h7AReC6tJt4oWQIOEFOxU1QAGm0S_pDaoutAJzwt7kvAGgudSv2YkE0DXVKdus-iGmKXoeujm2fDumCVNP_xzvBjfNI2aeAvc4PkTvOh6HPIcQfcTB77kbWj7dYxy5yzn56KaYBr6L0z3vcLijRtrOTQf4LXsVXJfx3bGfsZ9fPv-4-lrcfr--uVrdFl4pAYWrRFUh6mpdNsbIoE1tZONV3QQtoZYNYAjQSmF0icatEY3SPoCqggfhannGisU373A7r-12jL0b9za5aI-jX_RCq0xdgSK-eZanc7T_RI9CIRtFFxaCtJ8WLYG_Z8yT7WP22HVuwDRnW4KRmmIQh7U-_odu0jwOdAlbilIR1VQH6nyh_JhyHjE8rSPAHsK2FLb9GzaxH46O87rH9ol8TJeAywXYxQ73zzvZ1bebxfIP7Dm0vg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2124710956</pqid></control><display><type>article</type><title>Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency</title><source>Access via Wiley Online Library</source><creator>Govia, Rachelle N. M. ; Birse, Kenzie D. ; Sepehri, Shadi ; Khafipour, Ehsan ; Menticoglou, Savas M. ; Burgener, Adam D. ; Poliquin, Vanessa</creator><creatorcontrib>Govia, Rachelle N. M. ; Birse, Kenzie D. ; Sepehri, Shadi ; Khafipour, Ehsan ; Menticoglou, Savas M. ; Burgener, Adam D. ; Poliquin, Vanessa</creatorcontrib><description>Problem
Cervical insufficiency is a precursor of preterm birth. Treatment with emergency cervical cerclage is contraindicated in the presence of intra‐amniotic infection. Detecting infection with Gram stain and culture of amniotic fluid lacks sensitivity. Proteomic profiling of amniotic fluid in cervical insufficiency may help identify pregnancies best suited for emergency cerclage.
Method of study
Thirty‐two pregnant women underwent amniocentesis for routine genetic testing (n = 22) or after diagnosis of cervical insufficiency (n = 10). The proteomic profiles of the amniotic fluid samples were compared in a cross‐sectional fashion, including sub‐analyses of women with cervical insufficiency and latency periods of <1 week and >1 week post‐diagnosis.
Results
Mean gestational age at diagnosis of cervical insufficiency was 21.4 weeks (95% CI 20.6‐22.1). Proteomic analysis yielded 40 (7.2%, P < 0.05) differentially expressed proteins between women with delivery <1 week (n = 6) vs. >1 week (n = 4). Women who delivered <1 week had activated inflammatory response (z = 2.3, P = 6.71E−09), chemotaxis of immune cells (z = 2.9, P = 2.01E−08), and inhibited bacterial growth (z = ‐2.2, P = 5.82E−05). A multivariate model of eight biomarkers positively associated with cases of <1 week latency and distinguished cases from controls (97.8%, cross‐validation accuracy 92.7%, P = 0.0009).
Conclusion
In this pilot study, significant differences in the amniotic fluid proteomic profiles in cases of cervical insufficiency compared to genetic amniocentesis were observed. Proteomic signatures were predictive of achieving latency > 1 week after diagnosis of cervical insufficiency. These preliminary findings suggest that proteomic analysis may be of value in predicting outcome following cervical insufficiency and warrants further validation in larger studies.</description><identifier>ISSN: 1046-7408</identifier><identifier>ISSN: 1600-0897</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/aji.13030</identifier><identifier>PMID: 30076666</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Amniocentesis ; Amniotic fluid ; Cell culture ; cervical cerclage ; Chemotaxis ; chorioamnionitis ; Diagnosis ; Genetic screening ; Gestational age ; Gram stain ; Human papillomavirus ; infection ; Inflammation ; Latency ; Medicin och hälsovetenskap ; Premature birth ; Prenatal development ; proteomics ; Sutures</subject><ispartof>American journal of reproductive immunology (1989), 2018-11, Vol.80 (5), p.e13030-n/a</ispartof><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4410-a5155ee75b29883f786839c469f7306390eff0d31872e8abee847cf045fc01a63</citedby><cites>FETCH-LOGICAL-c4410-a5155ee75b29883f786839c469f7306390eff0d31872e8abee847cf045fc01a63</cites><orcidid>0000-0003-4673-7633 ; 0000-0001-8905-5590 ; 0000-0002-2303-5738 ; 0000-0003-0526-3902</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faji.13030$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faji.13030$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30076666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:139489711$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Govia, Rachelle N. M.</creatorcontrib><creatorcontrib>Birse, Kenzie D.</creatorcontrib><creatorcontrib>Sepehri, Shadi</creatorcontrib><creatorcontrib>Khafipour, Ehsan</creatorcontrib><creatorcontrib>Menticoglou, Savas M.</creatorcontrib><creatorcontrib>Burgener, Adam D.</creatorcontrib><creatorcontrib>Poliquin, Vanessa</creatorcontrib><title>Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
Cervical insufficiency is a precursor of preterm birth. Treatment with emergency cervical cerclage is contraindicated in the presence of intra‐amniotic infection. Detecting infection with Gram stain and culture of amniotic fluid lacks sensitivity. Proteomic profiling of amniotic fluid in cervical insufficiency may help identify pregnancies best suited for emergency cerclage.
Method of study
Thirty‐two pregnant women underwent amniocentesis for routine genetic testing (n = 22) or after diagnosis of cervical insufficiency (n = 10). The proteomic profiles of the amniotic fluid samples were compared in a cross‐sectional fashion, including sub‐analyses of women with cervical insufficiency and latency periods of <1 week and >1 week post‐diagnosis.
Results
Mean gestational age at diagnosis of cervical insufficiency was 21.4 weeks (95% CI 20.6‐22.1). Proteomic analysis yielded 40 (7.2%, P < 0.05) differentially expressed proteins between women with delivery <1 week (n = 6) vs. >1 week (n = 4). Women who delivered <1 week had activated inflammatory response (z = 2.3, P = 6.71E−09), chemotaxis of immune cells (z = 2.9, P = 2.01E−08), and inhibited bacterial growth (z = ‐2.2, P = 5.82E−05). A multivariate model of eight biomarkers positively associated with cases of <1 week latency and distinguished cases from controls (97.8%, cross‐validation accuracy 92.7%, P = 0.0009).
Conclusion
In this pilot study, significant differences in the amniotic fluid proteomic profiles in cases of cervical insufficiency compared to genetic amniocentesis were observed. Proteomic signatures were predictive of achieving latency > 1 week after diagnosis of cervical insufficiency. These preliminary findings suggest that proteomic analysis may be of value in predicting outcome following cervical insufficiency and warrants further validation in larger studies.</description><subject>Amniocentesis</subject><subject>Amniotic fluid</subject><subject>Cell culture</subject><subject>cervical cerclage</subject><subject>Chemotaxis</subject><subject>chorioamnionitis</subject><subject>Diagnosis</subject><subject>Genetic screening</subject><subject>Gestational age</subject><subject>Gram stain</subject><subject>Human papillomavirus</subject><subject>infection</subject><subject>Inflammation</subject><subject>Latency</subject><subject>Medicin och hälsovetenskap</subject><subject>Premature birth</subject><subject>Prenatal development</subject><subject>proteomics</subject><subject>Sutures</subject><issn>1046-7408</issn><issn>1600-0897</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUFv1DAQhS0EoqVw4A8gS1zoIe04dmLnuKqgFFXiAmfL64xbL0m8xElX---ZJdsiIXUuY4--9zSax9h7AReC6tJt4oWQIOEFOxU1QAGm0S_pDaoutAJzwt7kvAGgudSv2YkE0DXVKdus-iGmKXoeujm2fDumCVNP_xzvBjfNI2aeAvc4PkTvOh6HPIcQfcTB77kbWj7dYxy5yzn56KaYBr6L0z3vcLijRtrOTQf4LXsVXJfx3bGfsZ9fPv-4-lrcfr--uVrdFl4pAYWrRFUh6mpdNsbIoE1tZONV3QQtoZYNYAjQSmF0icatEY3SPoCqggfhannGisU373A7r-12jL0b9za5aI-jX_RCq0xdgSK-eZanc7T_RI9CIRtFFxaCtJ8WLYG_Z8yT7WP22HVuwDRnW4KRmmIQh7U-_odu0jwOdAlbilIR1VQH6nyh_JhyHjE8rSPAHsK2FLb9GzaxH46O87rH9ol8TJeAywXYxQ73zzvZ1bebxfIP7Dm0vg</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Govia, Rachelle N. M.</creator><creator>Birse, Kenzie D.</creator><creator>Sepehri, Shadi</creator><creator>Khafipour, Ehsan</creator><creator>Menticoglou, Savas M.</creator><creator>Burgener, Adam D.</creator><creator>Poliquin, Vanessa</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><orcidid>https://orcid.org/0000-0003-4673-7633</orcidid><orcidid>https://orcid.org/0000-0001-8905-5590</orcidid><orcidid>https://orcid.org/0000-0002-2303-5738</orcidid><orcidid>https://orcid.org/0000-0003-0526-3902</orcidid></search><sort><creationdate>201811</creationdate><title>Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency</title><author>Govia, Rachelle N. M. ; Birse, Kenzie D. ; Sepehri, Shadi ; Khafipour, Ehsan ; Menticoglou, Savas M. ; Burgener, Adam D. ; Poliquin, Vanessa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4410-a5155ee75b29883f786839c469f7306390eff0d31872e8abee847cf045fc01a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amniocentesis</topic><topic>Amniotic fluid</topic><topic>Cell culture</topic><topic>cervical cerclage</topic><topic>Chemotaxis</topic><topic>chorioamnionitis</topic><topic>Diagnosis</topic><topic>Genetic screening</topic><topic>Gestational age</topic><topic>Gram stain</topic><topic>Human papillomavirus</topic><topic>infection</topic><topic>Inflammation</topic><topic>Latency</topic><topic>Medicin och hälsovetenskap</topic><topic>Premature birth</topic><topic>Prenatal development</topic><topic>proteomics</topic><topic>Sutures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Govia, Rachelle N. M.</creatorcontrib><creatorcontrib>Birse, Kenzie D.</creatorcontrib><creatorcontrib>Sepehri, Shadi</creatorcontrib><creatorcontrib>Khafipour, Ehsan</creatorcontrib><creatorcontrib>Menticoglou, Savas M.</creatorcontrib><creatorcontrib>Burgener, Adam D.</creatorcontrib><creatorcontrib>Poliquin, Vanessa</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Govia, Rachelle N. M.</au><au>Birse, Kenzie D.</au><au>Sepehri, Shadi</au><au>Khafipour, Ehsan</au><au>Menticoglou, Savas M.</au><au>Burgener, Adam D.</au><au>Poliquin, Vanessa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2018-11</date><risdate>2018</risdate><volume>80</volume><issue>5</issue><spage>e13030</spage><epage>n/a</epage><pages>e13030-n/a</pages><issn>1046-7408</issn><issn>1600-0897</issn><eissn>1600-0897</eissn><abstract>Problem
Cervical insufficiency is a precursor of preterm birth. Treatment with emergency cervical cerclage is contraindicated in the presence of intra‐amniotic infection. Detecting infection with Gram stain and culture of amniotic fluid lacks sensitivity. Proteomic profiling of amniotic fluid in cervical insufficiency may help identify pregnancies best suited for emergency cerclage.
Method of study
Thirty‐two pregnant women underwent amniocentesis for routine genetic testing (n = 22) or after diagnosis of cervical insufficiency (n = 10). The proteomic profiles of the amniotic fluid samples were compared in a cross‐sectional fashion, including sub‐analyses of women with cervical insufficiency and latency periods of <1 week and >1 week post‐diagnosis.
Results
Mean gestational age at diagnosis of cervical insufficiency was 21.4 weeks (95% CI 20.6‐22.1). Proteomic analysis yielded 40 (7.2%, P < 0.05) differentially expressed proteins between women with delivery <1 week (n = 6) vs. >1 week (n = 4). Women who delivered <1 week had activated inflammatory response (z = 2.3, P = 6.71E−09), chemotaxis of immune cells (z = 2.9, P = 2.01E−08), and inhibited bacterial growth (z = ‐2.2, P = 5.82E−05). A multivariate model of eight biomarkers positively associated with cases of <1 week latency and distinguished cases from controls (97.8%, cross‐validation accuracy 92.7%, P = 0.0009).
Conclusion
In this pilot study, significant differences in the amniotic fluid proteomic profiles in cases of cervical insufficiency compared to genetic amniocentesis were observed. Proteomic signatures were predictive of achieving latency > 1 week after diagnosis of cervical insufficiency. These preliminary findings suggest that proteomic analysis may be of value in predicting outcome following cervical insufficiency and warrants further validation in larger studies.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30076666</pmid><doi>10.1111/aji.13030</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4673-7633</orcidid><orcidid>https://orcid.org/0000-0001-8905-5590</orcidid><orcidid>https://orcid.org/0000-0002-2303-5738</orcidid><orcidid>https://orcid.org/0000-0003-0526-3902</orcidid></addata></record> |
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| ispartof | American journal of reproductive immunology (1989), 2018-11, Vol.80 (5), p.e13030-n/a |
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| source | Access via Wiley Online Library |
| subjects | Amniocentesis Amniotic fluid Cell culture cervical cerclage Chemotaxis chorioamnionitis Diagnosis Genetic screening Gestational age Gram stain Human papillomavirus infection Inflammation Latency Medicin och hälsovetenskap Premature birth Prenatal development proteomics Sutures |
| title | Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency |
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