Effect of psychiatric drugs on Daphnia magna oxylipin profiles
Neuro-active pharmaceuticals have been reported to act as endocrine disruptors enhancing reproduction in the model crustacean Daphnia manga at environmental concentrations of ng/L. Oxylipins and more specifically eicosanoids, which are lipid mediators formed from polyunsaturated fatty acids (PUFAs),...
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| Veröffentlicht in: | The Science of the total environment 2018-12, Vol.644, p.1101-1109 |
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| creator | Garreta-Lara, Elba Checa, Antonio Fuchs, David Tauler, Romà Lacorte, Silvia Wheelock, Craig E. Barata, Carlos |
| description | Neuro-active pharmaceuticals have been reported to act as endocrine disruptors enhancing reproduction in the model crustacean Daphnia manga at environmental concentrations of ng/L. Oxylipins and more specifically eicosanoids, which are lipid mediators formed from polyunsaturated fatty acids (PUFAs), are known to regulate reproduction together with other physiological processes in insects. In D. magna, the biosynthesis of eicosanoids and their putative role in the regulation of reproduction has been studied using transcriptomics, genomics and exposures to cyclooxygenase inhibitors. Quantification of eicosanoids and oxylipins derived from PUFAs upon exposure to pharmaceuticals is therefore crucial for a better understanding of the mode of action of neuro-active pharmaceuticals on aquatic invertebrates. The aim of this study was to investigate shifts in the oxylipin profile in D. magna adults upon exposure to environmental concentrations of the three psychiatric drugs, fluoxetine, diazepam and carbamazepine, with known effects of enhancing offspring production. Oxylipin profiles were determined in whole organism tissues using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Up to 28 different oxylipins belonging to arachidonic (AA), linoleic acid (LA), α-linoleic acid (α-LA) and eicosapentaenoic acid (EPA) pathways were detected and quantified in D. magna adults. Exposure to the selected psychiatric drugs showed that fluoxetine enhanced the accumulation of the cyclooxygenase (COX) product 12-hydroxyheptadecatrienoic acid (12-HHTrE), whereas diazepam increased the concentration of eicosanoids belonging to the lipoxygenase (LOX) and cytochrome P450 (CYP) pathways (HETEs, EpOMEs, HODEs, HOTrEs and HEPEs) from the AA, LA, αLA and EPA pathways. Carbamazepine had little effect and only one LA-derived compound from the LOX pathway (13-HODE) increased significantly. This means that despite having different modes of action in humans, fluoxetine and diazepam up-regulated eicosanoid pathways in D. magna, closely related to known biologically active products that regulate reproduction in insects.
[Display omitted]
•Oxylipin profiles in D. magna adults upon exposure to low concentrations of psychiatric drugs were studied.•Up to 28 different oxylipins belonging to arachidonic, linoleic and eicosapentaenoic acid metabolic pathways were altered.•Fluoxetine increased the accumulation of the prostaglandin product 12-HHTrE.•Diazepam increased the c |
| doi_str_mv | 10.1016/j.scitotenv.2018.06.333 |
| format | Article |
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[Display omitted]
•Oxylipin profiles in D. magna adults upon exposure to low concentrations of psychiatric drugs were studied.•Up to 28 different oxylipins belonging to arachidonic, linoleic and eicosapentaenoic acid metabolic pathways were altered.•Fluoxetine increased the accumulation of the prostaglandin product 12-HHTrE.•Diazepam increased the concentration of 12 eicosanoids and other oxylipins.•Carbamazepine had little effects only effecting one oxylipin from the linoleic acid metabolism.</description><identifier>ISSN: 0048-9697</identifier><identifier>ISSN: 1879-1026</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2018.06.333</identifier><identifier>PMID: 30743823</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Daphnia - drug effects ; Daphnia - physiology ; Daphnia magna ; Eicosanoids ; Eicosapentaenoic Acid - analogs & derivatives ; Fatty Acids, Unsaturated ; Linoleic Acid ; Medicin och hälsovetenskap ; Oxylipin profiles ; Oxylipins - metabolism ; Psychiatric drugs ; Psychotropic Drugs - toxicity ; Target analysis ; UPLC-MS/MS ; Water Pollutants, Chemical - toxicity</subject><ispartof>The Science of the total environment, 2018-12, Vol.644, p.1101-1109</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-9b030cd00ec3d04a042607d6141cf122a81d1ff2098f4de36d6d670aabf935aa3</citedby><cites>FETCH-LOGICAL-c508t-9b030cd00ec3d04a042607d6141cf122a81d1ff2098f4de36d6d670aabf935aa3</cites><orcidid>0000-0002-3360-0729 ; 0000-0002-0674-3329</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.scitotenv.2018.06.333$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,309,310,314,780,784,789,790,885,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30743823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:139303770$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Garreta-Lara, Elba</creatorcontrib><creatorcontrib>Checa, Antonio</creatorcontrib><creatorcontrib>Fuchs, David</creatorcontrib><creatorcontrib>Tauler, Romà</creatorcontrib><creatorcontrib>Lacorte, Silvia</creatorcontrib><creatorcontrib>Wheelock, Craig E.</creatorcontrib><creatorcontrib>Barata, Carlos</creatorcontrib><title>Effect of psychiatric drugs on Daphnia magna oxylipin profiles</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Neuro-active pharmaceuticals have been reported to act as endocrine disruptors enhancing reproduction in the model crustacean Daphnia manga at environmental concentrations of ng/L. Oxylipins and more specifically eicosanoids, which are lipid mediators formed from polyunsaturated fatty acids (PUFAs), are known to regulate reproduction together with other physiological processes in insects. In D. magna, the biosynthesis of eicosanoids and their putative role in the regulation of reproduction has been studied using transcriptomics, genomics and exposures to cyclooxygenase inhibitors. Quantification of eicosanoids and oxylipins derived from PUFAs upon exposure to pharmaceuticals is therefore crucial for a better understanding of the mode of action of neuro-active pharmaceuticals on aquatic invertebrates. The aim of this study was to investigate shifts in the oxylipin profile in D. magna adults upon exposure to environmental concentrations of the three psychiatric drugs, fluoxetine, diazepam and carbamazepine, with known effects of enhancing offspring production. Oxylipin profiles were determined in whole organism tissues using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Up to 28 different oxylipins belonging to arachidonic (AA), linoleic acid (LA), α-linoleic acid (α-LA) and eicosapentaenoic acid (EPA) pathways were detected and quantified in D. magna adults. Exposure to the selected psychiatric drugs showed that fluoxetine enhanced the accumulation of the cyclooxygenase (COX) product 12-hydroxyheptadecatrienoic acid (12-HHTrE), whereas diazepam increased the concentration of eicosanoids belonging to the lipoxygenase (LOX) and cytochrome P450 (CYP) pathways (HETEs, EpOMEs, HODEs, HOTrEs and HEPEs) from the AA, LA, αLA and EPA pathways. Carbamazepine had little effect and only one LA-derived compound from the LOX pathway (13-HODE) increased significantly. This means that despite having different modes of action in humans, fluoxetine and diazepam up-regulated eicosanoid pathways in D. magna, closely related to known biologically active products that regulate reproduction in insects.
[Display omitted]
•Oxylipin profiles in D. magna adults upon exposure to low concentrations of psychiatric drugs were studied.•Up to 28 different oxylipins belonging to arachidonic, linoleic and eicosapentaenoic acid metabolic pathways were altered.•Fluoxetine increased the accumulation of the prostaglandin product 12-HHTrE.•Diazepam increased the concentration of 12 eicosanoids and other oxylipins.•Carbamazepine had little effects only effecting one oxylipin from the linoleic acid metabolism.</description><subject>Animals</subject><subject>Daphnia - drug effects</subject><subject>Daphnia - physiology</subject><subject>Daphnia magna</subject><subject>Eicosanoids</subject><subject>Eicosapentaenoic Acid - analogs & derivatives</subject><subject>Fatty Acids, Unsaturated</subject><subject>Linoleic Acid</subject><subject>Medicin och hälsovetenskap</subject><subject>Oxylipin profiles</subject><subject>Oxylipins - metabolism</subject><subject>Psychiatric drugs</subject><subject>Psychotropic Drugs - toxicity</subject><subject>Target analysis</subject><subject>UPLC-MS/MS</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0048-9697</issn><issn>1879-1026</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u3CAUhVGVqpmmfYXGy2zsXsADeBMpStIfKVI37RoxcEmYeowDdtJ5-zKayWRVFRYg9J170DmEnFNoKFDxed1kG6Y44fDUMKCqAdFwzt-QBVWyqykwcUIWAK2qO9HJU_I-5zWUJRV9R045yJYrxhfk8tZ7tFMVfTXmrX0IZkrBVi7N97mKQ3VjxochmGpj7gdTxT_bPoxhqMYUfegxfyBvvekzfjycZ-TXl9uf19_qux9fv19f3dV2CWqquxVwsA4ALXfQGmiZAOkEban1lDGjqKPeM-iUbx1y4cqWYMzKd3xpDD8j9X5ufsZxXukxhY1JWx1N0Ien3-WGulUMBC1890--_N29il6ElHccuJRQtBd7bQEfZ8yT3oRsse_NgHHOmpWIl1yUJAsq96hNMeeE_mhEQe-K0mt9LErvitIgdCmqKD8dTObVBt1R99JMAa72AJZYnwKm3SAcLLqQSmHaxfBfk79JnKox</recordid><startdate>20181210</startdate><enddate>20181210</enddate><creator>Garreta-Lara, Elba</creator><creator>Checa, Antonio</creator><creator>Fuchs, David</creator><creator>Tauler, Romà</creator><creator>Lacorte, Silvia</creator><creator>Wheelock, Craig E.</creator><creator>Barata, Carlos</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>BNKNJ</scope><orcidid>https://orcid.org/0000-0002-3360-0729</orcidid><orcidid>https://orcid.org/0000-0002-0674-3329</orcidid></search><sort><creationdate>20181210</creationdate><title>Effect of psychiatric drugs on Daphnia magna oxylipin profiles</title><author>Garreta-Lara, Elba ; Checa, Antonio ; Fuchs, David ; Tauler, Romà ; Lacorte, Silvia ; Wheelock, Craig E. ; Barata, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-9b030cd00ec3d04a042607d6141cf122a81d1ff2098f4de36d6d670aabf935aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Daphnia - drug effects</topic><topic>Daphnia - physiology</topic><topic>Daphnia magna</topic><topic>Eicosanoids</topic><topic>Eicosapentaenoic Acid - analogs & derivatives</topic><topic>Fatty Acids, Unsaturated</topic><topic>Linoleic Acid</topic><topic>Medicin och hälsovetenskap</topic><topic>Oxylipin profiles</topic><topic>Oxylipins - metabolism</topic><topic>Psychiatric drugs</topic><topic>Psychotropic Drugs - toxicity</topic><topic>Target analysis</topic><topic>UPLC-MS/MS</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garreta-Lara, Elba</creatorcontrib><creatorcontrib>Checa, Antonio</creatorcontrib><creatorcontrib>Fuchs, David</creatorcontrib><creatorcontrib>Tauler, Romà</creatorcontrib><creatorcontrib>Lacorte, Silvia</creatorcontrib><creatorcontrib>Wheelock, Craig E.</creatorcontrib><creatorcontrib>Barata, Carlos</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SwePub Conference</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garreta-Lara, Elba</au><au>Checa, Antonio</au><au>Fuchs, David</au><au>Tauler, Romà</au><au>Lacorte, Silvia</au><au>Wheelock, Craig E.</au><au>Barata, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of psychiatric drugs on Daphnia magna oxylipin profiles</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2018-12-10</date><risdate>2018</risdate><volume>644</volume><spage>1101</spage><epage>1109</epage><pages>1101-1109</pages><issn>0048-9697</issn><issn>1879-1026</issn><eissn>1879-1026</eissn><abstract>Neuro-active pharmaceuticals have been reported to act as endocrine disruptors enhancing reproduction in the model crustacean Daphnia manga at environmental concentrations of ng/L. Oxylipins and more specifically eicosanoids, which are lipid mediators formed from polyunsaturated fatty acids (PUFAs), are known to regulate reproduction together with other physiological processes in insects. In D. magna, the biosynthesis of eicosanoids and their putative role in the regulation of reproduction has been studied using transcriptomics, genomics and exposures to cyclooxygenase inhibitors. Quantification of eicosanoids and oxylipins derived from PUFAs upon exposure to pharmaceuticals is therefore crucial for a better understanding of the mode of action of neuro-active pharmaceuticals on aquatic invertebrates. The aim of this study was to investigate shifts in the oxylipin profile in D. magna adults upon exposure to environmental concentrations of the three psychiatric drugs, fluoxetine, diazepam and carbamazepine, with known effects of enhancing offspring production. Oxylipin profiles were determined in whole organism tissues using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Up to 28 different oxylipins belonging to arachidonic (AA), linoleic acid (LA), α-linoleic acid (α-LA) and eicosapentaenoic acid (EPA) pathways were detected and quantified in D. magna adults. Exposure to the selected psychiatric drugs showed that fluoxetine enhanced the accumulation of the cyclooxygenase (COX) product 12-hydroxyheptadecatrienoic acid (12-HHTrE), whereas diazepam increased the concentration of eicosanoids belonging to the lipoxygenase (LOX) and cytochrome P450 (CYP) pathways (HETEs, EpOMEs, HODEs, HOTrEs and HEPEs) from the AA, LA, αLA and EPA pathways. Carbamazepine had little effect and only one LA-derived compound from the LOX pathway (13-HODE) increased significantly. This means that despite having different modes of action in humans, fluoxetine and diazepam up-regulated eicosanoid pathways in D. magna, closely related to known biologically active products that regulate reproduction in insects.
[Display omitted]
•Oxylipin profiles in D. magna adults upon exposure to low concentrations of psychiatric drugs were studied.•Up to 28 different oxylipins belonging to arachidonic, linoleic and eicosapentaenoic acid metabolic pathways were altered.•Fluoxetine increased the accumulation of the prostaglandin product 12-HHTrE.•Diazepam increased the concentration of 12 eicosanoids and other oxylipins.•Carbamazepine had little effects only effecting one oxylipin from the linoleic acid metabolism.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30743823</pmid><doi>10.1016/j.scitotenv.2018.06.333</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-3360-0729</orcidid><orcidid>https://orcid.org/0000-0002-0674-3329</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Daphnia - drug effects Daphnia - physiology Daphnia magna Eicosanoids Eicosapentaenoic Acid - analogs & derivatives Fatty Acids, Unsaturated Linoleic Acid Medicin och hälsovetenskap Oxylipin profiles Oxylipins - metabolism Psychiatric drugs Psychotropic Drugs - toxicity Target analysis UPLC-MS/MS Water Pollutants, Chemical - toxicity |
| title | Effect of psychiatric drugs on Daphnia magna oxylipin profiles |
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