The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms
Aim To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism...
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| Veröffentlicht in: | International endodontic journal 2020-01, Vol.53 (1), p.53-61 |
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| container_title | International endodontic journal |
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| creator | Ali, I. A. A. Cheung, B. P. K. Yau, J. Y. Y. Matinlinna, J. P. Lévesque, C. M. Belibasakis, G. N. Neelakantan, P. |
| description | Aim
To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
Methodology
Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3‐day and 21‐day‐old biofilms. Unconditioned discs served as controls. The biofilms were analysed using culture‐dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony‐forming units and the biofilm matrix composition (polysaccharides and proteins), respectively. Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
Results
Collagen conditioning significantly increased the number of CFUs in the 21‐day biofilms, compared to the 3‐day biofilms (P |
| doi_str_mv | 10.1111/iej.13202 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_477742</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2322129986</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4762-85ec9b729c74cab268bce7636370e4d39c6f1b2fba313e702208592a793006d83</originalsourceid><addsrcrecordid>eNp1kUFv1DAQhS0EotvCgT-AInGhh7T2OInjI6oWKKrEpZwtxxm3XhJ7sRNV_fc4zW6RkDqXGY2-98byI-QDoxcs16XD3QXjQOEV2TDe1CXUkr0mG8oqXkLb1ifkNKUdpbSmnL0lJ5xVtGVSbsh0e4-F83aY0Rssgi3S3KUp6gnzFK3OSxN87yYXvPN3hfZ90blg3TAW-i4rfDHdL8y4D-mJWky2fsIYTDBmToXVaPTg0lGX3pE3Vg8J3x_6Gfn1dXt79b28-fnt-urLTWkq0UDZ1mhkJ0AaURndQdN2BkXDGy4oVj2XprGsA9tpzjgKCkDbWoIWklPa9C0_I-Xqmx5wP3dqH92o46MK2qnD6neeUFVCiAoyL1_k9zH0_0RHIauYaCmvl1ufV20G_8yYJjW6ZHAYtMcwJwUgQFQ5keXMp__QXZijzz-hgAMwkLJtMnW-UiaGlCLa5-cwqpbYVY5dPcWe2Y8Hx7kbsX8mjzln4HIFHtyAjy87qevtj9XyL0efuAE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2322129986</pqid></control><display><type>article</type><title>The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Ali, I. A. A. ; Cheung, B. P. K. ; Yau, J. Y. Y. ; Matinlinna, J. P. ; Lévesque, C. M. ; Belibasakis, G. N. ; Neelakantan, P.</creator><creatorcontrib>Ali, I. A. A. ; Cheung, B. P. K. ; Yau, J. Y. Y. ; Matinlinna, J. P. ; Lévesque, C. M. ; Belibasakis, G. N. ; Neelakantan, P.</creatorcontrib><description>Aim
To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
Methodology
Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3‐day and 21‐day‐old biofilms. Unconditioned discs served as controls. The biofilms were analysed using culture‐dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony‐forming units and the biofilm matrix composition (polysaccharides and proteins), respectively. Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
Results
Collagen conditioning significantly increased the number of CFUs in the 21‐day biofilms, compared to the 3‐day biofilms (P < 0.05). Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21‐day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21‐day biofilms, compared to the 3‐day biofilms (P < 0.05).
Conclusions
The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.</description><identifier>ISSN: 0143-2885</identifier><identifier>ISSN: 1365-2591</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.13202</identifier><identifier>PMID: 31408199</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Age ; Biochemical analysis ; biofilm ; Biofilms ; Cell culture ; Collagen ; Confocal microscopy ; Dentistry ; Endodontics ; Enterococcus faecalis ; EPS matrix ; Extracellular matrix ; Hydroxyapatite ; Medicin och hälsovetenskap ; Microscopy, Confocal ; Polysaccharides ; Saliva ; serum ; Statistical analysis</subject><ispartof>International endodontic journal, 2020-01, Vol.53 (1), p.53-61</ispartof><rights>2019 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2019 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4762-85ec9b729c74cab268bce7636370e4d39c6f1b2fba313e702208592a793006d83</citedby><cites>FETCH-LOGICAL-c4762-85ec9b729c74cab268bce7636370e4d39c6f1b2fba313e702208592a793006d83</cites><orcidid>0000-0003-3025-7598</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.13202$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.13202$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31408199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:141780358$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, I. A. A.</creatorcontrib><creatorcontrib>Cheung, B. P. K.</creatorcontrib><creatorcontrib>Yau, J. Y. Y.</creatorcontrib><creatorcontrib>Matinlinna, J. P.</creatorcontrib><creatorcontrib>Lévesque, C. M.</creatorcontrib><creatorcontrib>Belibasakis, G. N.</creatorcontrib><creatorcontrib>Neelakantan, P.</creatorcontrib><title>The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
Methodology
Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3‐day and 21‐day‐old biofilms. Unconditioned discs served as controls. The biofilms were analysed using culture‐dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony‐forming units and the biofilm matrix composition (polysaccharides and proteins), respectively. Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
Results
Collagen conditioning significantly increased the number of CFUs in the 21‐day biofilms, compared to the 3‐day biofilms (P < 0.05). Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21‐day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21‐day biofilms, compared to the 3‐day biofilms (P < 0.05).
Conclusions
The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.</description><subject>Age</subject><subject>Biochemical analysis</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Cell culture</subject><subject>Collagen</subject><subject>Confocal microscopy</subject><subject>Dentistry</subject><subject>Endodontics</subject><subject>Enterococcus faecalis</subject><subject>EPS matrix</subject><subject>Extracellular matrix</subject><subject>Hydroxyapatite</subject><subject>Medicin och hälsovetenskap</subject><subject>Microscopy, Confocal</subject><subject>Polysaccharides</subject><subject>Saliva</subject><subject>serum</subject><subject>Statistical analysis</subject><issn>0143-2885</issn><issn>1365-2591</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFv1DAQhS0EotvCgT-AInGhh7T2OInjI6oWKKrEpZwtxxm3XhJ7sRNV_fc4zW6RkDqXGY2-98byI-QDoxcs16XD3QXjQOEV2TDe1CXUkr0mG8oqXkLb1ifkNKUdpbSmnL0lJ5xVtGVSbsh0e4-F83aY0Rssgi3S3KUp6gnzFK3OSxN87yYXvPN3hfZ90blg3TAW-i4rfDHdL8y4D-mJWky2fsIYTDBmToXVaPTg0lGX3pE3Vg8J3x_6Gfn1dXt79b28-fnt-urLTWkq0UDZ1mhkJ0AaURndQdN2BkXDGy4oVj2XprGsA9tpzjgKCkDbWoIWklPa9C0_I-Xqmx5wP3dqH92o46MK2qnD6neeUFVCiAoyL1_k9zH0_0RHIauYaCmvl1ufV20G_8yYJjW6ZHAYtMcwJwUgQFQ5keXMp__QXZijzz-hgAMwkLJtMnW-UiaGlCLa5-cwqpbYVY5dPcWe2Y8Hx7kbsX8mjzln4HIFHtyAjy87qevtj9XyL0efuAE</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Ali, I. A. A.</creator><creator>Cheung, B. P. K.</creator><creator>Yau, J. Y. Y.</creator><creator>Matinlinna, J. P.</creator><creator>Lévesque, C. M.</creator><creator>Belibasakis, G. N.</creator><creator>Neelakantan, P.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><orcidid>https://orcid.org/0000-0003-3025-7598</orcidid></search><sort><creationdate>202001</creationdate><title>The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms</title><author>Ali, I. A. A. ; Cheung, B. P. K. ; Yau, J. Y. Y. ; Matinlinna, J. P. ; Lévesque, C. M. ; Belibasakis, G. N. ; Neelakantan, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4762-85ec9b729c74cab268bce7636370e4d39c6f1b2fba313e702208592a793006d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Age</topic><topic>Biochemical analysis</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Cell culture</topic><topic>Collagen</topic><topic>Confocal microscopy</topic><topic>Dentistry</topic><topic>Endodontics</topic><topic>Enterococcus faecalis</topic><topic>EPS matrix</topic><topic>Extracellular matrix</topic><topic>Hydroxyapatite</topic><topic>Medicin och hälsovetenskap</topic><topic>Microscopy, Confocal</topic><topic>Polysaccharides</topic><topic>Saliva</topic><topic>serum</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, I. A. A.</creatorcontrib><creatorcontrib>Cheung, B. P. K.</creatorcontrib><creatorcontrib>Yau, J. Y. Y.</creatorcontrib><creatorcontrib>Matinlinna, J. P.</creatorcontrib><creatorcontrib>Lévesque, C. M.</creatorcontrib><creatorcontrib>Belibasakis, G. N.</creatorcontrib><creatorcontrib>Neelakantan, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, I. A. A.</au><au>Cheung, B. P. K.</au><au>Yau, J. Y. Y.</au><au>Matinlinna, J. P.</au><au>Lévesque, C. M.</au><au>Belibasakis, G. N.</au><au>Neelakantan, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2020-01</date><risdate>2020</risdate><volume>53</volume><issue>1</issue><spage>53</spage><epage>61</epage><pages>53-61</pages><issn>0143-2885</issn><issn>1365-2591</issn><eissn>1365-2591</eissn><abstract>Aim
To investigate the null hypothesis that neither the surface conditioning (collagen, serum, saliva) of hydroxyapatite (HA) discs, nor the biofilm age (3 days vs. 21 days) has a significant effect on the cellular and matrix composition of biofilms, using Enterococcus faecalis as the model organism.
Methodology
Sterile HA discs were conditioned with collagen, saliva or serum, and inoculated with E. faecalis to form 3‐day and 21‐day‐old biofilms. Unconditioned discs served as controls. The biofilms were analysed using culture‐dependent and independent (confocal microscopy and biochemical analysis) methods, to determine the colony‐forming units and the biofilm matrix composition (polysaccharides and proteins), respectively. Statistical analyses were performed using appropriate parametric and nonparametric tests (P = 0.05).
Results
Collagen conditioning significantly increased the number of CFUs in the 21‐day biofilms, compared to the 3‐day biofilms (P < 0.05). Although the biochemical analysis revealed that surface conditioning had no significant effect on the total carbohydrate content in the 21‐day biofilms, confocal microscopic analysis revealed that collagen and saliva conditioning selectively increased the polysaccharide content of 21‐day biofilms, compared to the 3‐day biofilms (P < 0.05).
Conclusions
The results of this study raise an important methodological concern that the substrate conditioning substances and biofilm age differentially influence the cellular and extracellular matrix components of E. faecalis biofilms.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31408199</pmid><doi>10.1111/iej.13202</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3025-7598</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0143-2885 |
| ispartof | International endodontic journal, 2020-01, Vol.53 (1), p.53-61 |
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| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Age Biochemical analysis biofilm Biofilms Cell culture Collagen Confocal microscopy Dentistry Endodontics Enterococcus faecalis EPS matrix Extracellular matrix Hydroxyapatite Medicin och hälsovetenskap Microscopy, Confocal Polysaccharides Saliva serum Statistical analysis |
| title | The influence of substrate surface conditioning and biofilm age on the composition of Enterococcus faecalis biofilms |
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