Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity

RNA-guided CRISPR-Cas9 proteins have been widely used for genome editing, but their off-target activities limit broad application. The minimal Cas9 ortholog from Staphylococcus aureus (SaCas9) is commonly used for in vivo genome editing; however, no variant conferring high genome-wide specificity is...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2019-10, Vol.116 (42), p.20969-20976
Hauptverfasser:	Tan, Yuanyan, Chu, Athena H. Y., Bao, Siyu, Hoang, Duc Anh, Kebede, Firaol Tamiru, Xiong, Wenjun, Ji, Mingfang, Shi, Jiahai, Zheng, Zongli
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biological Sciences CRISPR CRISPR-Associated Protein 9 - chemistry CRISPR-Associated Protein 9 - genetics CRISPR-Associated Protein 9 - metabolism Editing Epithelium Gene Editing Gene sequencing Genome Genomes Humans Nuclease Physical Sciences PNAS Plus Protein Engineering Retina Retinal pigment epithelium Ribonucleic acid RNA Staphylococcus aureus Staphylococcus aureus - chemistry Staphylococcus aureus - enzymology Staphylococcus aureus - genetics Viruses
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