Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects
[Figure: see text] Interleukin 25 (IL-25) is a ligand for IL-25 receptor (IL-25R) with apoptotic effect on breast cancer epithelial cells that are produced by peripheral blood mononuclear cells (PBMCs). In this study, we aimed to evaluate IL-25/IL-25R mRNA expression in PBMCs, and also investigate c...
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| Veröffentlicht in: | Journal of interferon & cytokine research 2020-03, Vol.40 (3), p.139-144 |
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| container_title | Journal of interferon & cytokine research |
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| creator | Barati, Mehdi Sinaeian, Maryam Shokrollahi Barough, Mahdieh Pak, Fatemeh Semnani, Vahid Kokhaei, Parviz Momtazi-Borojeni, Amir Abbas |
| description | [Figure: see text] Interleukin 25 (IL-25) is a ligand for IL-25 receptor (IL-25R) with apoptotic effect on breast cancer epithelial cells that are produced by peripheral blood mononuclear cells (PBMCs). In this study, we aimed to evaluate IL-25/IL-25R mRNA expression in PBMCs, and also investigate correlation of IL-25/IL-25R with tumor stages/grades in patients with breast cancer. PBMCs and serum were isolated from 30 patients with breast cancer and 18 normal subjects. ELISA test was conducted for IL-25 cytokine. Total RNA was isolated from 2 × 10
PBMCs and reverse transcribed to cDNA. Quantitative PCRs were performed for IL-25, IL-25R, and GAPDH genes. IL-25 mRNA expression in PBMCs of breast cancer patients (malignant and benign) was significantly lower than that in normal subjects, Also IL-25 expression in breast cancer patients with malignant tumor was significantly lower than that in nonmalignant patients. IL-25R expression in malignant patients was significantly higher than that of benign and normal subjects (
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| doi_str_mv | 10.1089/jir.2019.0106 |
| format | Article |
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PBMCs and reverse transcribed to cDNA. Quantitative PCRs were performed for IL-25, IL-25R, and GAPDH genes. IL-25 mRNA expression in PBMCs of breast cancer patients (malignant and benign) was significantly lower than that in normal subjects, Also IL-25 expression in breast cancer patients with malignant tumor was significantly lower than that in nonmalignant patients. IL-25R expression in malignant patients was significantly higher than that of benign and normal subjects (
< 0.05). IL-25 in serum of normal subjects was higher than that of benign and malignant patients. There was a direct association between IL-25R expression and tumor grade/stage of cancer. In conclusion, IL-25 seems as a potential prognostic factor in the serum of breast cancer patients and reduction of IL-25 is associated with a higher grade/stage of cancer.</description><identifier>ISSN: 1079-9907</identifier><identifier>ISSN: 1557-7465</identifier><identifier>EISSN: 1557-7465</identifier><identifier>DOI: 10.1089/jir.2019.0106</identifier><identifier>PMID: 31905037</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Apoptosis ; Benign ; Blood ; Breast cancer ; Color imagery ; Cytokines ; Epithelial cells ; Gene expression ; Glyceraldehyde-3-phosphate dehydrogenase ; Image processing ; Interleukins ; Leukocytes (mononuclear) ; Medicin och hälsovetenskap ; Peripheral blood mononuclear cells ; Tumors</subject><ispartof>Journal of interferon & cytokine research, 2020-03, Vol.40 (3), p.139-144</ispartof><rights>Copyright Mary Ann Liebert, Inc. Mar 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-59553d1eb7aa1f5c7fbf91481c30bd45160329e296fb60758ba48649ebc1e64e3</citedby><cites>FETCH-LOGICAL-c409t-59553d1eb7aa1f5c7fbf91481c30bd45160329e296fb60758ba48649ebc1e64e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31905037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:143088705$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Barati, Mehdi</creatorcontrib><creatorcontrib>Sinaeian, Maryam</creatorcontrib><creatorcontrib>Shokrollahi Barough, Mahdieh</creatorcontrib><creatorcontrib>Pak, Fatemeh</creatorcontrib><creatorcontrib>Semnani, Vahid</creatorcontrib><creatorcontrib>Kokhaei, Parviz</creatorcontrib><creatorcontrib>Momtazi-Borojeni, Amir Abbas</creatorcontrib><title>Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects</title><title>Journal of interferon & cytokine research</title><addtitle>J Interferon Cytokine Res</addtitle><description>[Figure: see text] Interleukin 25 (IL-25) is a ligand for IL-25 receptor (IL-25R) with apoptotic effect on breast cancer epithelial cells that are produced by peripheral blood mononuclear cells (PBMCs). In this study, we aimed to evaluate IL-25/IL-25R mRNA expression in PBMCs, and also investigate correlation of IL-25/IL-25R with tumor stages/grades in patients with breast cancer. PBMCs and serum were isolated from 30 patients with breast cancer and 18 normal subjects. ELISA test was conducted for IL-25 cytokine. Total RNA was isolated from 2 × 10
PBMCs and reverse transcribed to cDNA. Quantitative PCRs were performed for IL-25, IL-25R, and GAPDH genes. IL-25 mRNA expression in PBMCs of breast cancer patients (malignant and benign) was significantly lower than that in normal subjects, Also IL-25 expression in breast cancer patients with malignant tumor was significantly lower than that in nonmalignant patients. IL-25R expression in malignant patients was significantly higher than that of benign and normal subjects (
< 0.05). IL-25 in serum of normal subjects was higher than that of benign and malignant patients. There was a direct association between IL-25R expression and tumor grade/stage of cancer. In conclusion, IL-25 seems as a potential prognostic factor in the serum of breast cancer patients and reduction of IL-25 is associated with a higher grade/stage of cancer.</description><subject>Apoptosis</subject><subject>Benign</subject><subject>Blood</subject><subject>Breast cancer</subject><subject>Color imagery</subject><subject>Cytokines</subject><subject>Epithelial cells</subject><subject>Gene expression</subject><subject>Glyceraldehyde-3-phosphate dehydrogenase</subject><subject>Image processing</subject><subject>Interleukins</subject><subject>Leukocytes (mononuclear)</subject><subject>Medicin och hälsovetenskap</subject><subject>Peripheral blood mononuclear cells</subject><subject>Tumors</subject><issn>1079-9907</issn><issn>1557-7465</issn><issn>1557-7465</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kk1v1DAQhi0Eou3CkSuyxKWXLHb8FR_paoFKBSo-zpbtTES2WTvYCR-_pH8Xh25bqRInj2aed2Y0fhF6Qcmakka_3vVpXROq14QS-QgdUyFUpbgUj0tMlK60JuoIneS8I4TIptZP0RGjmgjC1DG63v60w2ynPgYcO3weJkgDzFd9wLXANrQPU5_BwzjFhLe_xwQ5L8JSuYTUj98h2QGfDTG2-EMMMcx-AJvwBoYhL-3PEtg84Y0NHhK-LGMhTPnfmI8x7Yv4y-x24Kf8DD3p7JDh-eFdoW9vt18376uLT-_ON28uKs-JniqhhWAtBaespZ3wqnOdpryhnhHXckElYbWGWsvOSaJE4yxvJNfgPAXJga1QddM3_4JxdmZM_d6mPyba3hxSVyUCwxXnWhRe_5cfU2zvRbdCyhlpGkUW7emNtoA_ZsiT2ffZl9vYAHHOpmaM17zh5WdW6NUDdBfnFMolCiWl1qqh8n55n2LOCbq7dSgxiztMcYdZ3GEWdxT-5aHr7PbQ3tG3dmB_AQigt4c</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Barati, Mehdi</creator><creator>Sinaeian, Maryam</creator><creator>Shokrollahi Barough, Mahdieh</creator><creator>Pak, Fatemeh</creator><creator>Semnani, Vahid</creator><creator>Kokhaei, Parviz</creator><creator>Momtazi-Borojeni, Amir Abbas</creator><general>Mary Ann Liebert, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20200301</creationdate><title>Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects</title><author>Barati, Mehdi ; Sinaeian, Maryam ; Shokrollahi Barough, Mahdieh ; Pak, Fatemeh ; Semnani, Vahid ; Kokhaei, Parviz ; Momtazi-Borojeni, Amir Abbas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-59553d1eb7aa1f5c7fbf91481c30bd45160329e296fb60758ba48649ebc1e64e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Benign</topic><topic>Blood</topic><topic>Breast cancer</topic><topic>Color imagery</topic><topic>Cytokines</topic><topic>Epithelial cells</topic><topic>Gene expression</topic><topic>Glyceraldehyde-3-phosphate dehydrogenase</topic><topic>Image processing</topic><topic>Interleukins</topic><topic>Leukocytes (mononuclear)</topic><topic>Medicin och hälsovetenskap</topic><topic>Peripheral blood mononuclear cells</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barati, Mehdi</creatorcontrib><creatorcontrib>Sinaeian, Maryam</creatorcontrib><creatorcontrib>Shokrollahi Barough, Mahdieh</creatorcontrib><creatorcontrib>Pak, Fatemeh</creatorcontrib><creatorcontrib>Semnani, Vahid</creatorcontrib><creatorcontrib>Kokhaei, Parviz</creatorcontrib><creatorcontrib>Momtazi-Borojeni, Amir Abbas</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Journal of interferon & cytokine research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barati, Mehdi</au><au>Sinaeian, Maryam</au><au>Shokrollahi Barough, Mahdieh</au><au>Pak, Fatemeh</au><au>Semnani, Vahid</au><au>Kokhaei, Parviz</au><au>Momtazi-Borojeni, Amir Abbas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects</atitle><jtitle>Journal of interferon & cytokine research</jtitle><addtitle>J Interferon Cytokine Res</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>40</volume><issue>3</issue><spage>139</spage><epage>144</epage><pages>139-144</pages><issn>1079-9907</issn><issn>1557-7465</issn><eissn>1557-7465</eissn><abstract>[Figure: see text] Interleukin 25 (IL-25) is a ligand for IL-25 receptor (IL-25R) with apoptotic effect on breast cancer epithelial cells that are produced by peripheral blood mononuclear cells (PBMCs). In this study, we aimed to evaluate IL-25/IL-25R mRNA expression in PBMCs, and also investigate correlation of IL-25/IL-25R with tumor stages/grades in patients with breast cancer. PBMCs and serum were isolated from 30 patients with breast cancer and 18 normal subjects. ELISA test was conducted for IL-25 cytokine. Total RNA was isolated from 2 × 10
PBMCs and reverse transcribed to cDNA. Quantitative PCRs were performed for IL-25, IL-25R, and GAPDH genes. IL-25 mRNA expression in PBMCs of breast cancer patients (malignant and benign) was significantly lower than that in normal subjects, Also IL-25 expression in breast cancer patients with malignant tumor was significantly lower than that in nonmalignant patients. IL-25R expression in malignant patients was significantly higher than that of benign and normal subjects (
< 0.05). IL-25 in serum of normal subjects was higher than that of benign and malignant patients. There was a direct association between IL-25R expression and tumor grade/stage of cancer. In conclusion, IL-25 seems as a potential prognostic factor in the serum of breast cancer patients and reduction of IL-25 is associated with a higher grade/stage of cancer.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>31905037</pmid><doi>10.1089/jir.2019.0106</doi><tpages>6</tpages></addata></record> |
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| subjects | Apoptosis Benign Blood Breast cancer Color imagery Cytokines Epithelial cells Gene expression Glyceraldehyde-3-phosphate dehydrogenase Image processing Interleukins Leukocytes (mononuclear) Medicin och hälsovetenskap Peripheral blood mononuclear cells Tumors |
| title | Evaluation of Interleukin 25 and Interleukin 25 Receptor Expression in Peripheral Blood Mononuclear Cells of Breast Cancer Patients and Normal Subjects |
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