A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols
Tuberculosis (TB) infects about 25% of the world population and claims more human lives than any other infectious disease. TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology co...
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| Veröffentlicht in: | Tuberculosis (Edinburgh, Scotland) Scotland), 2020-01, Vol.120, p.101896-101896, Article 101896 |
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| container_title | Tuberculosis (Edinburgh, Scotland) |
| container_volume | 120 |
| creator | Rufino de Sousa, Nuno Sandström, Niklas Shen, Lei Håkansson, Kathleen Vezozzo, Rafaella Udekwu, Klas I. Croda, Julio Rothfuchs, Antonio Gigliotti |
| description | Tuberculosis (TB) infects about 25% of the world population and claims more human lives than any other infectious disease. TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology could play an important role in TB control by enabling the detection of airborne M. tuberculosis, but tools that are easy to use and scalable in TB hotspots are lacking. We developed an electrostatic air sampler termed the TB Hotspot DetectOR (THOR) and investigated its performance in laboratory aerosol experiments and in a prison hotspot of TB transmission. We show that THOR collects aerosols carrying microspheres, Bacillus globigii spores and M. bovis BCG, concentrating these microparticles onto a collector piece designed for subsequent detection analysis. The unit was also successfully operated in the complex setting of a prison hotspot, enabling detection of a molecular signature for M. tuberculosis in the cough of inmates. Future deployment of this device may lead to a measurable impact on TB case-finding by screening individuals through the aerosols they generate. |
| doi_str_mv | 10.1016/j.tube.2019.101896 |
| format | Article |
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TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology could play an important role in TB control by enabling the detection of airborne M. tuberculosis, but tools that are easy to use and scalable in TB hotspots are lacking. We developed an electrostatic air sampler termed the TB Hotspot DetectOR (THOR) and investigated its performance in laboratory aerosol experiments and in a prison hotspot of TB transmission. We show that THOR collects aerosols carrying microspheres, Bacillus globigii spores and M. bovis BCG, concentrating these microparticles onto a collector piece designed for subsequent detection analysis. The unit was also successfully operated in the complex setting of a prison hotspot, enabling detection of a molecular signature for M. tuberculosis in the cough of inmates. Future deployment of this device may lead to a measurable impact on TB case-finding by screening individuals through the aerosols they generate.</description><identifier>ISSN: 1472-9792</identifier><identifier>ISSN: 1873-281X</identifier><identifier>EISSN: 1873-281X</identifier><identifier>DOI: 10.1016/j.tube.2019.101896</identifier><identifier>PMID: 32090857</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Aerosols ; Air sampling ; Bacillus Calmette-Guerin vaccine ; BCG ; Bioaerosols ; Cough ; Diagnostics ; Disease hot spots ; Disease transmission ; Electrostatic properties ; Infectious diseases ; Inhalation ; Lung diseases ; Microparticles ; Microspheres ; Pathogen detection ; Prisons ; Respiration ; Spores ; Tuberculosis ; World population</subject><ispartof>Tuberculosis (Edinburgh, Scotland), 2020-01, Vol.120, p.101896-101896, Article 101896</ispartof><rights>2019 The Authors</rights><rights>Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. 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TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology could play an important role in TB control by enabling the detection of airborne M. tuberculosis, but tools that are easy to use and scalable in TB hotspots are lacking. We developed an electrostatic air sampler termed the TB Hotspot DetectOR (THOR) and investigated its performance in laboratory aerosol experiments and in a prison hotspot of TB transmission. We show that THOR collects aerosols carrying microspheres, Bacillus globigii spores and M. bovis BCG, concentrating these microparticles onto a collector piece designed for subsequent detection analysis. The unit was also successfully operated in the complex setting of a prison hotspot, enabling detection of a molecular signature for M. tuberculosis in the cough of inmates. Future deployment of this device may lead to a measurable impact on TB case-finding by screening individuals through the aerosols they generate.</description><subject>Aerosols</subject><subject>Air sampling</subject><subject>Bacillus Calmette-Guerin vaccine</subject><subject>BCG</subject><subject>Bioaerosols</subject><subject>Cough</subject><subject>Diagnostics</subject><subject>Disease hot spots</subject><subject>Disease transmission</subject><subject>Electrostatic properties</subject><subject>Infectious diseases</subject><subject>Inhalation</subject><subject>Lung diseases</subject><subject>Microparticles</subject><subject>Microspheres</subject><subject>Pathogen detection</subject><subject>Prisons</subject><subject>Respiration</subject><subject>Spores</subject><subject>Tuberculosis</subject><subject>World population</subject><issn>1472-9792</issn><issn>1873-281X</issn><issn>1873-281X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>D8T</sourceid><recordid>eNp9kl-L1DAUxYMo7rr6BXyQgi8-2DFJ26QBEYb1Lyz4sopvIU1uZzObaWaTdsVv7y0dB1dwnxKS3zm5ufcQ8pzRFaNMvNmuxqmDFadMzQetEg_IKWtlVfKW_XiI-1ryUknFT8iTnLcURbSlj8lJxamibSNPyeW66D0EZ7oABQSwY4p5NKO3hfGpyGa3D5AKGBDww6aYX0x2CjH7XDgYUeDjUPih6Hw0gOIY8lPyqDchw7PDeka-ffxwef65vPj66cv5-qK0jRJj6RivQLHacOWUbWsLold15TrX9FwyLNIKwUA60RjX9lijtK5hjrGeMitYdUbKxTf_hP3U6X3yO5N-6Wi8Phxd4w50LSteC-Rf_5d_77-vdUwbnSfNWirq-l77I349Xmku53Yi_27hEd6BszCMyYQ7srs3g7_Sm3irJa2VohINXh0MUryZII9657OFEMwAccqaV6LCsTWUIvryH3QbpzRgt5GSUtJKyZniC2VxMDlBfyyGUT1nSG_1PFE9Z0gvGULRi7-_cZT8CQ0CbxcAcLa3HpLO1sNgwfmEedAu-vv8fwMQWtrI</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Rufino de Sousa, Nuno</creator><creator>Sandström, Niklas</creator><creator>Shen, Lei</creator><creator>Håkansson, Kathleen</creator><creator>Vezozzo, Rafaella</creator><creator>Udekwu, Klas I.</creator><creator>Croda, Julio</creator><creator>Rothfuchs, Antonio Gigliotti</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><general>Churchill Livingstone</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AFDQA</scope><scope>AOWAS</scope><scope>D8T</scope><scope>D8V</scope><scope>ZZAVC</scope><scope>ABAVF</scope><scope>DG7</scope></search><sort><creationdate>202001</creationdate><title>A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols</title><author>Rufino de Sousa, Nuno ; 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TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology could play an important role in TB control by enabling the detection of airborne M. tuberculosis, but tools that are easy to use and scalable in TB hotspots are lacking. We developed an electrostatic air sampler termed the TB Hotspot DetectOR (THOR) and investigated its performance in laboratory aerosol experiments and in a prison hotspot of TB transmission. We show that THOR collects aerosols carrying microspheres, Bacillus globigii spores and M. bovis BCG, concentrating these microparticles onto a collector piece designed for subsequent detection analysis. The unit was also successfully operated in the complex setting of a prison hotspot, enabling detection of a molecular signature for M. tuberculosis in the cough of inmates. Future deployment of this device may lead to a measurable impact on TB case-finding by screening individuals through the aerosols they generate.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>32090857</pmid><doi>10.1016/j.tube.2019.101896</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Tuberculosis (Edinburgh, Scotland), 2020-01, Vol.120, p.101896-101896, Article 101896 |
| issn | 1472-9792 1873-281X 1873-281X |
| language | eng |
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| source | Elsevier ScienceDirect Journals; SWEPUB Freely available online |
| subjects | Aerosols Air sampling Bacillus Calmette-Guerin vaccine BCG Bioaerosols Cough Diagnostics Disease hot spots Disease transmission Electrostatic properties Infectious diseases Inhalation Lung diseases Microparticles Microspheres Pathogen detection Prisons Respiration Spores Tuberculosis World population |
| title | A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols |
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