Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity
To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in φ amplitude at 10 -10 Hz specifically c...
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| Veröffentlicht in: | Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2015-07, Vol.3 (25), p.4997-5000 |
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| container_issue | 25 |
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| container_title | Journal of materials chemistry. B, Materials for biology and medicine |
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| creator | Löffler, S Richter-Dahlfors, A |
| description | To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in φ amplitude at 10
-10
Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy. |
| doi_str_mv | 10.1039/c5tb00381d |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 2050-750X |
| ispartof | Journal of materials chemistry. B, Materials for biology and medicine, 2015-07, Vol.3 (25), p.4997-5000 |
| issn | 2050-750X 2050-7518 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_472204 |
| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection; SWEPUB Freely available online |
| subjects | Barriers Density Disruption Formations Integrity Monitoring Real time Spectroscopy |
| title | Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity |
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