CRISPR-assisted detection of RNA–protein interactions in living cells

We have developed CRISPR-assisted RNA–protein interaction detection method (CARPID), which leverages CRISPR–CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs) in the native cellular context. We applied CARPID to the nuclear lncRNA...

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Veröffentlicht in:	Nature methods 2020-07, Vol.17 (7), p.685-688
Hauptverfasser:	Yi, Wenkai, Li, Jingyu, Zhu, Xiaoxuan, Wang, Xi, Fan, Ligang, Sun, Wenju, Liao, Linbu, Zhang, Jilin, Li, Xiaoyu, Ye, Jing, Chen, Fulin, Taipale, Jussi, Chan, Kui Ming, Zhang, Liang, Yan, Jian
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2067 631/1647/2210 631/1647/2217 631/208/191 Animals Binders Binding Binding proteins Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biotin Brief Communication Cells (biology) Clustered Regularly Interspaced Short Palindromic Repeats CRISPR DNA-Binding Proteins - metabolism HEK293 Cells Humans Labeling Life Sciences Ligases Medicin och hälsovetenskap Non-coding RNA Protein binding Protein interaction Proteins Proteomics Ribonucleic acid RNA RNA, Long Noncoding - metabolism RNA-binding protein RNA-Binding Proteins - metabolism TATA-Binding Protein Associated Factors - metabolism Transcription Factors - metabolism
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