Assessment of In Vitro and In Silico Protocols for Sequence-Based Characterization of the Human Vaginal Microbiome

The vaginal microbiome has been connected to a wide range of health outcomes. This has led to a thriving research environment but also to the use of conflicting methodologies to study its microbial composition. Here, we systematically assessed best practices for the sequencing-based characterization...

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Veröffentlicht in:	MSPHERE 2020-11, Vol.5 (6), p.e00448
Hauptverfasser:	Hugerth, Luisa W, Pereira, Marcela, Zha, Yinghua, Seifert, Maike, Kaldhusdal, Vilde, Boulund, Fredrik, Krog, Maria C, Bashir, Zahra, Hamsten, Marica, Fransson, Emma, Svarre-Nielsen, Henriette, Schuppe-Koistinen, Ina, Engstrand, Lars
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rRNA amplicon Host-Microbe Biology human microbiome metagenomics molecular methods PCR quantitative methods vaginal microbiome
Online-Zugang:	Volltext
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