Cellular Trafficking of Glutathione Transferase M2-2 Between U373MG and SHSY-S7 Cells is Mediated by Exosomes
The enzyme glutathione transferase M2-2, expressed in human astrocytes, increases its expression in the presence of aminochrome and catalyzes the conjugation of aminochrome, preventing its toxic effects. Secretion of the enzyme glutathione transferase M2-2 from U373MG cells, used as a cellular model...
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| Veröffentlicht in: | Neurotoxicity research 2021-04, Vol.39 (2), p.182-190 |
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| creator | Valdes, Raúl Armijo, Alicia Muñoz, Patricia Hultenby, Kjell Hagg, Andres Inzunza, Jose Nalvarte, Ivan Varshney, Mukesh Mannervik, Bengt Segura-Aguilar, Juan |
| description | The enzyme glutathione transferase M2-2, expressed in human astrocytes, increases its expression in the presence of aminochrome and catalyzes the conjugation of aminochrome, preventing its toxic effects. Secretion of the enzyme glutathione transferase M2-2 from U373MG cells, used as a cellular model for astrocytes, has been reported, and the enzyme is taken up by neuroblastoma SYSH-S7 cells and provide protection against aminochrome. The present study provides evidence that glutathione transferase M2-2 is released in exosomes from U373MG cells, thereby providing a means for intercellular transport of the enzyme. With particular relevance to Parkinson disease and other degenerative conditions, we propose a new mechanism by which astrocytes may protect dopaminergic neurons against the endogenous neurotoxin aminochrome. |
| doi_str_mv | 10.1007/s12640-020-00327-5 |
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Secretion of the enzyme glutathione transferase M2-2 from U373MG cells, used as a cellular model for astrocytes, has been reported, and the enzyme is taken up by neuroblastoma SYSH-S7 cells and provide protection against aminochrome. The present study provides evidence that glutathione transferase M2-2 is released in exosomes from U373MG cells, thereby providing a means for intercellular transport of the enzyme. With particular relevance to Parkinson disease and other degenerative conditions, we propose a new mechanism by which astrocytes may protect dopaminergic neurons against the endogenous neurotoxin aminochrome.</description><subject>Aminochrome</subject><subject>Astrocytes</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Dopamine</subject><subject>Dopaminergic neurons</subject><subject>Exosomes</subject><subject>Medicin och hälsovetenskap</subject><subject>Neurobiology</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neuroprotection</subject><subject>Neurosciences</subject><subject>Original Article</subject><subject>Parkinson´s disease</subject><subject>Pharmacology/Toxicology</subject><issn>1029-8428</issn><issn>1476-3524</issn><issn>1476-3524</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kc1O3DAUhS3Uit--QBeVH6Cm9vVfsqQDHSoxYjGA1JXlJPY0kIlHdiLK2-MwA12BJdtXvuc7tnwQ-sroKaNU_0gMlKCEQp6UgyZyDx0yoRXhEsSnXFMoSSGgOEBHKd1TCkwqvY8OOJd5CHWI1jPXdWNnI76J1vu2fmj7FQ4ez7txsMPfNvRuavXJu2iTwwsggH-64dG5Ht9yzRdzbPsGLy-Xf8hS48kv4TbhhWtaO7gGV0_44l9IYe3SCfrsbZfcl91-jG5_XdzMLsnV9fz37OyK1ELDQErNfF3x2jfKFnVpG1VA5b3koLTijCqotFKyUBpK0ShPq4LlxVegqa-Y4MeIbH3To9uMldnEdm3jkwm2Nbujh1w5I5SQdNKX7-o3MTT_oVeQCVmUgnPI7Pd32fP27syEuDJpNKwEWU5y2MrrGFKKzr8BjJopVrON1eRYzUusRmbo2xbKF6xd84a85pgFfPeI3OpXLpr7MMY-__FHts8aua1u</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Valdes, Raúl</creator><creator>Armijo, Alicia</creator><creator>Muñoz, Patricia</creator><creator>Hultenby, Kjell</creator><creator>Hagg, Andres</creator><creator>Inzunza, Jose</creator><creator>Nalvarte, Ivan</creator><creator>Varshney, Mukesh</creator><creator>Mannervik, Bengt</creator><creator>Segura-Aguilar, Juan</creator><general>Springer US</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DG7</scope><orcidid>https://orcid.org/0000-0001-6596-9451</orcidid><orcidid>https://orcid.org/0000-0001-6828-2583</orcidid><orcidid>https://orcid.org/0000-0003-0876-6767</orcidid><orcidid>https://orcid.org/0000-0003-2165-0152</orcidid><orcidid>https://orcid.org/0000-0002-6416-064X</orcidid><orcidid>https://orcid.org/0000-0002-1018-673X</orcidid></search><sort><creationdate>20210401</creationdate><title>Cellular Trafficking of Glutathione Transferase M2-2 Between U373MG and SHSY-S7 Cells is Mediated by Exosomes</title><author>Valdes, Raúl ; Armijo, Alicia ; Muñoz, Patricia ; Hultenby, Kjell ; Hagg, Andres ; Inzunza, Jose ; Nalvarte, Ivan ; Varshney, Mukesh ; Mannervik, Bengt ; Segura-Aguilar, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-971fcb3cfd6a8c9ad682bff53267631062b7665867294d6f0b81f0bfb270fb143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aminochrome</topic><topic>Astrocytes</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Dopamine</topic><topic>Dopaminergic neurons</topic><topic>Exosomes</topic><topic>Medicin och hälsovetenskap</topic><topic>Neurobiology</topic><topic>Neurochemistry</topic><topic>Neurology</topic><topic>Neuroprotection</topic><topic>Neurosciences</topic><topic>Original Article</topic><topic>Parkinson´s disease</topic><topic>Pharmacology/Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valdes, Raúl</creatorcontrib><creatorcontrib>Armijo, Alicia</creatorcontrib><creatorcontrib>Muñoz, Patricia</creatorcontrib><creatorcontrib>Hultenby, Kjell</creatorcontrib><creatorcontrib>Hagg, Andres</creatorcontrib><creatorcontrib>Inzunza, Jose</creatorcontrib><creatorcontrib>Nalvarte, Ivan</creatorcontrib><creatorcontrib>Varshney, Mukesh</creatorcontrib><creatorcontrib>Mannervik, Bengt</creatorcontrib><creatorcontrib>Segura-Aguilar, Juan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Stockholms universitet</collection><jtitle>Neurotoxicity research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valdes, Raúl</au><au>Armijo, Alicia</au><au>Muñoz, Patricia</au><au>Hultenby, Kjell</au><au>Hagg, Andres</au><au>Inzunza, Jose</au><au>Nalvarte, Ivan</au><au>Varshney, Mukesh</au><au>Mannervik, Bengt</au><au>Segura-Aguilar, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular Trafficking of Glutathione Transferase M2-2 Between U373MG and SHSY-S7 Cells is Mediated by Exosomes</atitle><jtitle>Neurotoxicity research</jtitle><stitle>Neurotox Res</stitle><addtitle>Neurotox Res</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>39</volume><issue>2</issue><spage>182</spage><epage>190</epage><pages>182-190</pages><issn>1029-8428</issn><issn>1476-3524</issn><eissn>1476-3524</eissn><abstract>The enzyme glutathione transferase M2-2, expressed in human astrocytes, increases its expression in the presence of aminochrome and catalyzes the conjugation of aminochrome, preventing its toxic effects. 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| subjects | Aminochrome Astrocytes Biomedical and Life Sciences Biomedicine Cell Biology Dopamine Dopaminergic neurons Exosomes Medicin och hälsovetenskap Neurobiology Neurochemistry Neurology Neuroprotection Neurosciences Original Article Parkinson´s disease Pharmacology/Toxicology |
| title | Cellular Trafficking of Glutathione Transferase M2-2 Between U373MG and SHSY-S7 Cells is Mediated by Exosomes |
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