Cerebrospinal fluid and serum protein markers in autism: A co‐twin study
No robust biomarkers have yet been identified for autism spectrum disorder (ASD) or autistic traits. Familial factors likely influence biomarkers such as protein concentrations. Comparing twins with ASD or high autistic traits to the less affected co‐twin allows estimating the impact of familial con...
Gespeichert in:
| Veröffentlicht in: | Journal of neurochemistry 2021-08, Vol.158 (3), p.798-806 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 806 |
|---|---|
| container_issue | 3 |
| container_start_page | 798 |
| container_title | Journal of neurochemistry |
| container_volume | 158 |
| creator | Smedler, Erik Kleppe, Johanna Neufeld, Janina Lundin, Karl Bölte, Sven Landén, Mikael |
| description | No robust biomarkers have yet been identified for autism spectrum disorder (ASD) or autistic traits. Familial factors likely influence biomarkers such as protein concentrations. Comparing twins with ASD or high autistic traits to the less affected co‐twin allows estimating the impact of familial confounding. We measured 203 proteins in cerebrospinal fluid (n = 86) and serum (n = 127) in twins (mean age 14.2 years, 44.9% females) enriched for ASD and other neurodevelopmental conditions. Autistic traits were assessed by using the parent‐report version of the Social Responsiveness Scale‐2. In cerebrospinal fluid, autistic traits correlated negatively with three proteins and positively with one. In serum, autistic traits correlated positively with 15 and negatively with one. Also in serum, six were positively—and one negatively—associated with ASD. A pathway analysis of these proteins revealed immune system enrichment. In within twin pair analyses, autistic traits were associated with serum B‐cell activating factor (BAFF) only, whereas Cystatin B (CSTB) remained significantly associated with ASD. These associations did not remain significant when only considering monozygotic twins. For the remainder, the within‐pair analysis indicated familial confounding, including shared environment and genes, influencing both autism and protein levels. Our findings indicate proteins involved in immunity as putative biomarkers of autistic traits and ASD with partial genetic confounding. Although some results are in line with previous studies in general, further studies are needed for replication.
Using a twin cohort enriched for autism spectrum disorder (ASD) and other neurodevelopmental conditions, we report putative serum and cerebrospinal fluid (CSF) biomarkers for ASD involved in immunity. Especially, we highlight B‐cell activating factor (BAFF) and Cystatin B (CSTB), that withstood correction for shared genetics. TD, typically developing. ADHD, attention deficit hyperactivity disorder. |
| doi_str_mv | 10.1111/jnc.15338 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_463781</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2560200568</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5148-4f4b1fef61af942ea90f538fc069b21691a5cf19f33076a91da01fc67a913baf3</originalsourceid><addsrcrecordid>eNp9kU1OwzAQhS0EglJYcAEUiRWLFE_8k4QdqvgVgg2sLSexUUrbBDtW1R1H4IychClpYQXeeDT63ht7HiFHQEeA52wyL0cgGMu2yAB4CjEHkW-TAaVJEjPKkz2y7_2EUpBcwi7ZY0ymQrB0QO7GxpnCNb6t53oa2Wmoq0jPq8gbF2ZR65rO1PNopt2rcT7CUoeu9rPz6CIqm8_3j26BPd-FanlAdqyeenO4vofk-eryaXwT3z9e344v7uNSAM9ibnkB1lgJ2uY8MTqnVrDMllTmRQIyBy1KC7lljKZS51BpCraUKZas0JYNSdz7-oVpQ6FaV-PzlqrRtVq3XrEyikuWZoB8_ieP_6t-RRshoBKo5Nm_s15Cq7D1ElaS1ZazBPmTnkfjt2B8pyZNcLhZrxIhaUKpkCvX054qcfHeGfvjC1StElWYqPpOFNnjtWMoZqb6ITcRInDWA4t6apZ_O6m7h3Fv-QWqTayH</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2560200568</pqid></control><display><type>article</type><title>Cerebrospinal fluid and serum protein markers in autism: A co‐twin study</title><source>SWEPUB Freely available online</source><source>Access via Wiley Online Library</source><source>IngentaConnect Free/Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>Free Full-Text Journals in Chemistry</source><creator>Smedler, Erik ; Kleppe, Johanna ; Neufeld, Janina ; Lundin, Karl ; Bölte, Sven ; Landén, Mikael</creator><creatorcontrib>Smedler, Erik ; Kleppe, Johanna ; Neufeld, Janina ; Lundin, Karl ; Bölte, Sven ; Landén, Mikael</creatorcontrib><description>No robust biomarkers have yet been identified for autism spectrum disorder (ASD) or autistic traits. Familial factors likely influence biomarkers such as protein concentrations. Comparing twins with ASD or high autistic traits to the less affected co‐twin allows estimating the impact of familial confounding. We measured 203 proteins in cerebrospinal fluid (n = 86) and serum (n = 127) in twins (mean age 14.2 years, 44.9% females) enriched for ASD and other neurodevelopmental conditions. Autistic traits were assessed by using the parent‐report version of the Social Responsiveness Scale‐2. In cerebrospinal fluid, autistic traits correlated negatively with three proteins and positively with one. In serum, autistic traits correlated positively with 15 and negatively with one. Also in serum, six were positively—and one negatively—associated with ASD. A pathway analysis of these proteins revealed immune system enrichment. In within twin pair analyses, autistic traits were associated with serum B‐cell activating factor (BAFF) only, whereas Cystatin B (CSTB) remained significantly associated with ASD. These associations did not remain significant when only considering monozygotic twins. For the remainder, the within‐pair analysis indicated familial confounding, including shared environment and genes, influencing both autism and protein levels. Our findings indicate proteins involved in immunity as putative biomarkers of autistic traits and ASD with partial genetic confounding. Although some results are in line with previous studies in general, further studies are needed for replication.
Using a twin cohort enriched for autism spectrum disorder (ASD) and other neurodevelopmental conditions, we report putative serum and cerebrospinal fluid (CSF) biomarkers for ASD involved in immunity. Especially, we highlight B‐cell activating factor (BAFF) and Cystatin B (CSTB), that withstood correction for shared genetics. TD, typically developing. ADHD, attention deficit hyperactivity disorder.</description><identifier>ISSN: 0022-3042</identifier><identifier>ISSN: 1471-4159</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/jnc.15338</identifier><identifier>PMID: 33675537</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Autism ; Autism spectrum disorder ; autistic traits ; Biomarkers ; BLyS protein ; Cerebrospinal fluid ; Clinical Medicine ; Cystatins ; environmental factors ; genetics ; Immune system ; Klinisk medicin ; Medicin och hälsovetenskap ; Proteins ; Psychiatry ; Psykiatri ; Serum proteins ; Twin studies ; Twins</subject><ispartof>Journal of neurochemistry, 2021-08, Vol.158 (3), p.798-806</ispartof><rights>2021 The Authors. published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry</rights><rights>2021 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry.</rights><rights>2021. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5148-4f4b1fef61af942ea90f538fc069b21691a5cf19f33076a91da01fc67a913baf3</citedby><cites>FETCH-LOGICAL-c5148-4f4b1fef61af942ea90f538fc069b21691a5cf19f33076a91da01fc67a913baf3</cites><orcidid>0000-0003-4609-3620</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjnc.15338$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjnc.15338$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33675537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://gup.ub.gu.se/publication/304282$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:146310648$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Smedler, Erik</creatorcontrib><creatorcontrib>Kleppe, Johanna</creatorcontrib><creatorcontrib>Neufeld, Janina</creatorcontrib><creatorcontrib>Lundin, Karl</creatorcontrib><creatorcontrib>Bölte, Sven</creatorcontrib><creatorcontrib>Landén, Mikael</creatorcontrib><title>Cerebrospinal fluid and serum protein markers in autism: A co‐twin study</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>No robust biomarkers have yet been identified for autism spectrum disorder (ASD) or autistic traits. Familial factors likely influence biomarkers such as protein concentrations. Comparing twins with ASD or high autistic traits to the less affected co‐twin allows estimating the impact of familial confounding. We measured 203 proteins in cerebrospinal fluid (n = 86) and serum (n = 127) in twins (mean age 14.2 years, 44.9% females) enriched for ASD and other neurodevelopmental conditions. Autistic traits were assessed by using the parent‐report version of the Social Responsiveness Scale‐2. In cerebrospinal fluid, autistic traits correlated negatively with three proteins and positively with one. In serum, autistic traits correlated positively with 15 and negatively with one. Also in serum, six were positively—and one negatively—associated with ASD. A pathway analysis of these proteins revealed immune system enrichment. In within twin pair analyses, autistic traits were associated with serum B‐cell activating factor (BAFF) only, whereas Cystatin B (CSTB) remained significantly associated with ASD. These associations did not remain significant when only considering monozygotic twins. For the remainder, the within‐pair analysis indicated familial confounding, including shared environment and genes, influencing both autism and protein levels. Our findings indicate proteins involved in immunity as putative biomarkers of autistic traits and ASD with partial genetic confounding. Although some results are in line with previous studies in general, further studies are needed for replication.
Using a twin cohort enriched for autism spectrum disorder (ASD) and other neurodevelopmental conditions, we report putative serum and cerebrospinal fluid (CSF) biomarkers for ASD involved in immunity. Especially, we highlight B‐cell activating factor (BAFF) and Cystatin B (CSTB), that withstood correction for shared genetics. TD, typically developing. ADHD, attention deficit hyperactivity disorder.</description><subject>Autism</subject><subject>Autism spectrum disorder</subject><subject>autistic traits</subject><subject>Biomarkers</subject><subject>BLyS protein</subject><subject>Cerebrospinal fluid</subject><subject>Clinical Medicine</subject><subject>Cystatins</subject><subject>environmental factors</subject><subject>genetics</subject><subject>Immune system</subject><subject>Klinisk medicin</subject><subject>Medicin och hälsovetenskap</subject><subject>Proteins</subject><subject>Psychiatry</subject><subject>Psykiatri</subject><subject>Serum proteins</subject><subject>Twin studies</subject><subject>Twins</subject><issn>0022-3042</issn><issn>1471-4159</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>D8T</sourceid><recordid>eNp9kU1OwzAQhS0EglJYcAEUiRWLFE_8k4QdqvgVgg2sLSexUUrbBDtW1R1H4IychClpYQXeeDT63ht7HiFHQEeA52wyL0cgGMu2yAB4CjEHkW-TAaVJEjPKkz2y7_2EUpBcwi7ZY0ymQrB0QO7GxpnCNb6t53oa2Wmoq0jPq8gbF2ZR65rO1PNopt2rcT7CUoeu9rPz6CIqm8_3j26BPd-FanlAdqyeenO4vofk-eryaXwT3z9e344v7uNSAM9ibnkB1lgJ2uY8MTqnVrDMllTmRQIyBy1KC7lljKZS51BpCraUKZas0JYNSdz7-oVpQ6FaV-PzlqrRtVq3XrEyikuWZoB8_ieP_6t-RRshoBKo5Nm_s15Cq7D1ElaS1ZazBPmTnkfjt2B8pyZNcLhZrxIhaUKpkCvX054qcfHeGfvjC1StElWYqPpOFNnjtWMoZqb6ITcRInDWA4t6apZ_O6m7h3Fv-QWqTayH</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Smedler, Erik</creator><creator>Kleppe, Johanna</creator><creator>Neufeld, Janina</creator><creator>Lundin, Karl</creator><creator>Bölte, Sven</creator><creator>Landén, Mikael</creator><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>F1U</scope><scope>D8T</scope><scope>ZZAVC</scope><orcidid>https://orcid.org/0000-0003-4609-3620</orcidid></search><sort><creationdate>202108</creationdate><title>Cerebrospinal fluid and serum protein markers in autism: A co‐twin study</title><author>Smedler, Erik ; Kleppe, Johanna ; Neufeld, Janina ; Lundin, Karl ; Bölte, Sven ; Landén, Mikael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5148-4f4b1fef61af942ea90f538fc069b21691a5cf19f33076a91da01fc67a913baf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Autism</topic><topic>Autism spectrum disorder</topic><topic>autistic traits</topic><topic>Biomarkers</topic><topic>BLyS protein</topic><topic>Cerebrospinal fluid</topic><topic>Clinical Medicine</topic><topic>Cystatins</topic><topic>environmental factors</topic><topic>genetics</topic><topic>Immune system</topic><topic>Klinisk medicin</topic><topic>Medicin och hälsovetenskap</topic><topic>Proteins</topic><topic>Psychiatry</topic><topic>Psykiatri</topic><topic>Serum proteins</topic><topic>Twin studies</topic><topic>Twins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smedler, Erik</creatorcontrib><creatorcontrib>Kleppe, Johanna</creatorcontrib><creatorcontrib>Neufeld, Janina</creatorcontrib><creatorcontrib>Lundin, Karl</creatorcontrib><creatorcontrib>Bölte, Sven</creatorcontrib><creatorcontrib>Landén, Mikael</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Göteborgs universitet</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smedler, Erik</au><au>Kleppe, Johanna</au><au>Neufeld, Janina</au><au>Lundin, Karl</au><au>Bölte, Sven</au><au>Landén, Mikael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cerebrospinal fluid and serum protein markers in autism: A co‐twin study</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2021-08</date><risdate>2021</risdate><volume>158</volume><issue>3</issue><spage>798</spage><epage>806</epage><pages>798-806</pages><issn>0022-3042</issn><issn>1471-4159</issn><eissn>1471-4159</eissn><abstract>No robust biomarkers have yet been identified for autism spectrum disorder (ASD) or autistic traits. Familial factors likely influence biomarkers such as protein concentrations. Comparing twins with ASD or high autistic traits to the less affected co‐twin allows estimating the impact of familial confounding. We measured 203 proteins in cerebrospinal fluid (n = 86) and serum (n = 127) in twins (mean age 14.2 years, 44.9% females) enriched for ASD and other neurodevelopmental conditions. Autistic traits were assessed by using the parent‐report version of the Social Responsiveness Scale‐2. In cerebrospinal fluid, autistic traits correlated negatively with three proteins and positively with one. In serum, autistic traits correlated positively with 15 and negatively with one. Also in serum, six were positively—and one negatively—associated with ASD. A pathway analysis of these proteins revealed immune system enrichment. In within twin pair analyses, autistic traits were associated with serum B‐cell activating factor (BAFF) only, whereas Cystatin B (CSTB) remained significantly associated with ASD. These associations did not remain significant when only considering monozygotic twins. For the remainder, the within‐pair analysis indicated familial confounding, including shared environment and genes, influencing both autism and protein levels. Our findings indicate proteins involved in immunity as putative biomarkers of autistic traits and ASD with partial genetic confounding. Although some results are in line with previous studies in general, further studies are needed for replication.
Using a twin cohort enriched for autism spectrum disorder (ASD) and other neurodevelopmental conditions, we report putative serum and cerebrospinal fluid (CSF) biomarkers for ASD involved in immunity. Especially, we highlight B‐cell activating factor (BAFF) and Cystatin B (CSTB), that withstood correction for shared genetics. TD, typically developing. ADHD, attention deficit hyperactivity disorder.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>33675537</pmid><doi>10.1111/jnc.15338</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-4609-3620</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 2021-08, Vol.158 (3), p.798-806 |
| issn | 0022-3042 1471-4159 1471-4159 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_463781 |
| source | SWEPUB Freely available online; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Free Full-Text Journals in Chemistry |
| subjects | Autism Autism spectrum disorder autistic traits Biomarkers BLyS protein Cerebrospinal fluid Clinical Medicine Cystatins environmental factors genetics Immune system Klinisk medicin Medicin och hälsovetenskap Proteins Psychiatry Psykiatri Serum proteins Twin studies Twins |
| title | Cerebrospinal fluid and serum protein markers in autism: A co‐twin study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T20%3A58%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cerebrospinal%20fluid%20and%20serum%20protein%20markers%20in%20autism:%20A%20co%E2%80%90twin%20study&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Smedler,%20Erik&rft.date=2021-08&rft.volume=158&rft.issue=3&rft.spage=798&rft.epage=806&rft.pages=798-806&rft.issn=0022-3042&rft.eissn=1471-4159&rft_id=info:doi/10.1111/jnc.15338&rft_dat=%3Cproquest_swepu%3E2560200568%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2560200568&rft_id=info:pmid/33675537&rfr_iscdi=true |