Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies
As the global COVID-19 pandemic progresses, it is paramount to gain knowledge on adaptive immunity to SARS-CoV-2 in children to define immune correlates of protection upon immunization or infection. We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected...
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| Veröffentlicht in: | Cell reports (Cambridge) 2021-03, Vol.34 (11), p.108852-108852, Article 108852 |
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| creator | Cotugno, Nicola Ruggiero, Alessandra Bonfante, Francesco Petrara, Maria Raffaella Zicari, Sonia Pascucci, Giuseppe Rubens Zangari, Paola De Ioris, Maria Antonietta Santilli, Veronica Manno, E.C. Amodio, Donato Bortolami, Alessio Pagliari, Matteo Concato, Carlo Linardos, Giulia Campana, Andrea Donà, Daniele Giaquinto, Carlo Bernardi, Stefania Romani, Lorenza Pansa, Paola Chiurchiú, Sara Finocchi, Andrea Cancrini, Caterina Lancella, Laura Cursi, Laura De Luca, Maia Cutrera, Renato Sessa, Libera Morrocchi, Elena Medri, Chiara Putignani, Lorenza Calò Carducci, F.I. D’Argenio, Patrizia Ciofi degli Atti, Marta D’Amore, Carmen Piccioni, Livia Di Giuseppe, Martina Jenkner, Alessandro Giancotta, Carmela Krzysztofiak, Andrzej Brodin, Petter Rossi, Paolo De Rossi, Anita Palma, Paolo |
| description | As the global COVID-19 pandemic progresses, it is paramount to gain knowledge on adaptive immunity to SARS-CoV-2 in children to define immune correlates of protection upon immunization or infection. We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected children at 7 (±2) days after symptom onset. Individuals with specific humoral responses presented faster virus clearance and lower viral load associated with a reduced in vitro infectivity. We demonstrated that the frequencies of SARS-CoV-2-specific CD4+CD40L+ T cells and Spike-specific B cells were associated with the anti-SARS-CoV-2 antibodies and the magnitude of neutralizing activity. The plasma proteome confirmed the association between cellular and humoral SARS-CoV-2 immunity, and PRNT+ patients show higher viral signal transduction molecules (SLAMF1, CD244, CLEC4G). This work sheds lights on cellular and humoral anti-SARS-CoV-2 responses in children, which may drive future vaccination trial endpoints and quarantine measures policies.
[Display omitted]
•Antibody neutralization is inversely associated with SARS-CoV-2 in nasopharyngeal swabs•Antigen-specific B cells positively associate with anti-SARS-CoV-2 humoral immunity•SARS-CoV-2 neutralizing antibodies are present in patients with higher specific T cells•Antigen-specific B and T cells are features of SARS-CoV-2 immunity in children
Cotugno et al. show that neutralizing antibodies affect SARS-CoV-2 viral load in infected children. Antigen-specific B and T cells are positively associated with virus neutralization. This information provides a basis for defining SARS-CoV-2 adaptive responses in children. |
| doi_str_mv | 10.1016/j.celrep.2021.108852 |
| format | Article |
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[Display omitted]
•Antibody neutralization is inversely associated with SARS-CoV-2 in nasopharyngeal swabs•Antigen-specific B cells positively associate with anti-SARS-CoV-2 humoral immunity•SARS-CoV-2 neutralizing antibodies are present in patients with higher specific T cells•Antigen-specific B and T cells are features of SARS-CoV-2 immunity in children
Cotugno et al. show that neutralizing antibodies affect SARS-CoV-2 viral load in infected children. Antigen-specific B and T cells are positively associated with virus neutralization. This information provides a basis for defining SARS-CoV-2 adaptive responses in children.</description><identifier>ISSN: 2211-1247</identifier><identifier>EISSN: 2211-1247</identifier><identifier>DOI: 10.1016/j.celrep.2021.108852</identifier><identifier>PMID: 33730580</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Ab-mediated neutralization activity ; Adaptive Immunity - immunology ; anti-SARS-CoV-2 antibodies ; Antibodies, Neutralizing - immunology ; Antibodies, Viral - immunology ; antigen-specific B cells ; antigen-specific CD4 T cells ; B-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - immunology ; Child ; COVID-19 ; COVID-19 - immunology ; COVID-19 - virology ; Humans ; Immunity, Humoral - immunology ; pediatric COVID-19 ; Proteome - immunology ; proteomic profiling ; SARS-CoV-2 ; SARS-CoV-2 - immunology ; Signal Transduction - immunology ; Viral Load - immunology</subject><ispartof>Cell reports (Cambridge), 2021-03, Vol.34 (11), p.108852-108852, Article 108852</ispartof><rights>2021 The Author(s)</rights><rights>Copyright © 2021 The Author(s). Published by Elsevier Inc. 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We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected children at 7 (±2) days after symptom onset. Individuals with specific humoral responses presented faster virus clearance and lower viral load associated with a reduced in vitro infectivity. We demonstrated that the frequencies of SARS-CoV-2-specific CD4+CD40L+ T cells and Spike-specific B cells were associated with the anti-SARS-CoV-2 antibodies and the magnitude of neutralizing activity. The plasma proteome confirmed the association between cellular and humoral SARS-CoV-2 immunity, and PRNT+ patients show higher viral signal transduction molecules (SLAMF1, CD244, CLEC4G). This work sheds lights on cellular and humoral anti-SARS-CoV-2 responses in children, which may drive future vaccination trial endpoints and quarantine measures policies.
[Display omitted]
•Antibody neutralization is inversely associated with SARS-CoV-2 in nasopharyngeal swabs•Antigen-specific B cells positively associate with anti-SARS-CoV-2 humoral immunity•SARS-CoV-2 neutralizing antibodies are present in patients with higher specific T cells•Antigen-specific B and T cells are features of SARS-CoV-2 immunity in children
Cotugno et al. show that neutralizing antibodies affect SARS-CoV-2 viral load in infected children. Antigen-specific B and T cells are positively associated with virus neutralization. This information provides a basis for defining SARS-CoV-2 adaptive responses in children.</description><subject>Ab-mediated neutralization activity</subject><subject>Adaptive Immunity - immunology</subject><subject>anti-SARS-CoV-2 antibodies</subject><subject>Antibodies, Neutralizing - immunology</subject><subject>Antibodies, Viral - immunology</subject><subject>antigen-specific B cells</subject><subject>antigen-specific CD4 T cells</subject><subject>B-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>Child</subject><subject>COVID-19</subject><subject>COVID-19 - immunology</subject><subject>COVID-19 - virology</subject><subject>Humans</subject><subject>Immunity, Humoral - immunology</subject><subject>pediatric COVID-19</subject><subject>Proteome - immunology</subject><subject>proteomic profiling</subject><subject>SARS-CoV-2</subject><subject>SARS-CoV-2 - immunology</subject><subject>Signal Transduction - immunology</subject><subject>Viral Load - 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immunology</topic><topic>anti-SARS-CoV-2 antibodies</topic><topic>Antibodies, Neutralizing - immunology</topic><topic>Antibodies, Viral - immunology</topic><topic>antigen-specific B cells</topic><topic>antigen-specific CD4 T cells</topic><topic>B-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>Child</topic><topic>COVID-19</topic><topic>COVID-19 - immunology</topic><topic>COVID-19 - virology</topic><topic>Humans</topic><topic>Immunity, Humoral - immunology</topic><topic>pediatric COVID-19</topic><topic>Proteome - immunology</topic><topic>proteomic profiling</topic><topic>SARS-CoV-2</topic><topic>SARS-CoV-2 - immunology</topic><topic>Signal Transduction - immunology</topic><topic>Viral Load - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cotugno, Nicola</creatorcontrib><creatorcontrib>Ruggiero, Alessandra</creatorcontrib><creatorcontrib>Bonfante, Francesco</creatorcontrib><creatorcontrib>Petrara, Maria Raffaella</creatorcontrib><creatorcontrib>Zicari, Sonia</creatorcontrib><creatorcontrib>Pascucci, Giuseppe Rubens</creatorcontrib><creatorcontrib>Zangari, Paola</creatorcontrib><creatorcontrib>De Ioris, Maria Antonietta</creatorcontrib><creatorcontrib>Santilli, Veronica</creatorcontrib><creatorcontrib>Manno, E.C.</creatorcontrib><creatorcontrib>Amodio, Donato</creatorcontrib><creatorcontrib>Bortolami, Alessio</creatorcontrib><creatorcontrib>Pagliari, Matteo</creatorcontrib><creatorcontrib>Concato, Carlo</creatorcontrib><creatorcontrib>Linardos, Giulia</creatorcontrib><creatorcontrib>Campana, Andrea</creatorcontrib><creatorcontrib>Donà, Daniele</creatorcontrib><creatorcontrib>Giaquinto, Carlo</creatorcontrib><creatorcontrib>Bernardi, Stefania</creatorcontrib><creatorcontrib>Romani, Lorenza</creatorcontrib><creatorcontrib>Pansa, Paola</creatorcontrib><creatorcontrib>Chiurchiú, Sara</creatorcontrib><creatorcontrib>Finocchi, Andrea</creatorcontrib><creatorcontrib>Cancrini, Caterina</creatorcontrib><creatorcontrib>Lancella, Laura</creatorcontrib><creatorcontrib>Cursi, Laura</creatorcontrib><creatorcontrib>De Luca, Maia</creatorcontrib><creatorcontrib>Cutrera, Renato</creatorcontrib><creatorcontrib>Sessa, Libera</creatorcontrib><creatorcontrib>Morrocchi, Elena</creatorcontrib><creatorcontrib>Medri, Chiara</creatorcontrib><creatorcontrib>Putignani, Lorenza</creatorcontrib><creatorcontrib>Calò Carducci, F.I.</creatorcontrib><creatorcontrib>D’Argenio, Patrizia</creatorcontrib><creatorcontrib>Ciofi degli Atti, Marta</creatorcontrib><creatorcontrib>D’Amore, Carmen</creatorcontrib><creatorcontrib>Piccioni, Livia</creatorcontrib><creatorcontrib>Di Giuseppe, Martina</creatorcontrib><creatorcontrib>Jenkner, Alessandro</creatorcontrib><creatorcontrib>Giancotta, Carmela</creatorcontrib><creatorcontrib>Krzysztofiak, Andrzej</creatorcontrib><creatorcontrib>Brodin, Petter</creatorcontrib><creatorcontrib>Rossi, Paolo</creatorcontrib><creatorcontrib>De Rossi, Anita</creatorcontrib><creatorcontrib>Palma, Paolo</creatorcontrib><creatorcontrib>The CACTUS Study Team</creatorcontrib><creatorcontrib>CACTUS Study Team</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Cell reports (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cotugno, Nicola</au><au>Ruggiero, Alessandra</au><au>Bonfante, Francesco</au><au>Petrara, Maria Raffaella</au><au>Zicari, Sonia</au><au>Pascucci, Giuseppe Rubens</au><au>Zangari, Paola</au><au>De Ioris, Maria Antonietta</au><au>Santilli, Veronica</au><au>Manno, E.C.</au><au>Amodio, Donato</au><au>Bortolami, Alessio</au><au>Pagliari, Matteo</au><au>Concato, Carlo</au><au>Linardos, Giulia</au><au>Campana, Andrea</au><au>Donà, Daniele</au><au>Giaquinto, Carlo</au><au>Bernardi, Stefania</au><au>Romani, Lorenza</au><au>Pansa, Paola</au><au>Chiurchiú, Sara</au><au>Finocchi, Andrea</au><au>Cancrini, Caterina</au><au>Lancella, Laura</au><au>Cursi, Laura</au><au>De Luca, Maia</au><au>Cutrera, Renato</au><au>Sessa, Libera</au><au>Morrocchi, Elena</au><au>Medri, Chiara</au><au>Putignani, Lorenza</au><au>Calò Carducci, F.I.</au><au>D’Argenio, Patrizia</au><au>Ciofi degli Atti, Marta</au><au>D’Amore, Carmen</au><au>Piccioni, Livia</au><au>Di Giuseppe, Martina</au><au>Jenkner, Alessandro</au><au>Giancotta, Carmela</au><au>Krzysztofiak, Andrzej</au><au>Brodin, Petter</au><au>Rossi, Paolo</au><au>De Rossi, Anita</au><au>Palma, Paolo</au><aucorp>The CACTUS Study Team</aucorp><aucorp>CACTUS Study Team</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies</atitle><jtitle>Cell reports (Cambridge)</jtitle><addtitle>Cell Rep</addtitle><date>2021-03-16</date><risdate>2021</risdate><volume>34</volume><issue>11</issue><spage>108852</spage><epage>108852</epage><pages>108852-108852</pages><artnum>108852</artnum><issn>2211-1247</issn><eissn>2211-1247</eissn><abstract>As the global COVID-19 pandemic progresses, it is paramount to gain knowledge on adaptive immunity to SARS-CoV-2 in children to define immune correlates of protection upon immunization or infection. We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected children at 7 (±2) days after symptom onset. Individuals with specific humoral responses presented faster virus clearance and lower viral load associated with a reduced in vitro infectivity. We demonstrated that the frequencies of SARS-CoV-2-specific CD4+CD40L+ T cells and Spike-specific B cells were associated with the anti-SARS-CoV-2 antibodies and the magnitude of neutralizing activity. The plasma proteome confirmed the association between cellular and humoral SARS-CoV-2 immunity, and PRNT+ patients show higher viral signal transduction molecules (SLAMF1, CD244, CLEC4G). This work sheds lights on cellular and humoral anti-SARS-CoV-2 responses in children, which may drive future vaccination trial endpoints and quarantine measures policies.
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•Antibody neutralization is inversely associated with SARS-CoV-2 in nasopharyngeal swabs•Antigen-specific B cells positively associate with anti-SARS-CoV-2 humoral immunity•SARS-CoV-2 neutralizing antibodies are present in patients with higher specific T cells•Antigen-specific B and T cells are features of SARS-CoV-2 immunity in children
Cotugno et al. show that neutralizing antibodies affect SARS-CoV-2 viral load in infected children. Antigen-specific B and T cells are positively associated with virus neutralization. This information provides a basis for defining SARS-CoV-2 adaptive responses in children.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>33730580</pmid><doi>10.1016/j.celrep.2021.108852</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-5978-1193</orcidid><orcidid>https://orcid.org/0000-0003-4550-3018</orcidid><orcidid>https://orcid.org/0000-0003-1240-8057</orcidid><orcidid>https://orcid.org/0000-0002-1041-7489</orcidid><orcidid>https://orcid.org/0000-0001-6435-7509</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2211-1247 |
| ispartof | Cell reports (Cambridge), 2021-03, Vol.34 (11), p.108852-108852, Article 108852 |
| issn | 2211-1247 2211-1247 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; SWEPUB Freely available online |
| subjects | Ab-mediated neutralization activity Adaptive Immunity - immunology anti-SARS-CoV-2 antibodies Antibodies, Neutralizing - immunology Antibodies, Viral - immunology antigen-specific B cells antigen-specific CD4 T cells B-Lymphocytes - immunology CD4-Positive T-Lymphocytes - immunology Child COVID-19 COVID-19 - immunology COVID-19 - virology Humans Immunity, Humoral - immunology pediatric COVID-19 Proteome - immunology proteomic profiling SARS-CoV-2 SARS-CoV-2 - immunology Signal Transduction - immunology Viral Load - immunology |
| title | Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies |
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