Adsorption of Horseradish Peroxidase on Metallic Nanoparticles: Effects on Reactive Oxygen Species Detection Using 2′,7′-Dichlorofluorescin Diacetate

The fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA) together with the enzyme horseradish peroxidase (HRP) is widely used in nanotoxicology to study acellular reactive oxygen species (ROS) production from nanoparticles (NPs). This study examined whether HRP adsorbs onto NPs of Mn, Ni,...

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Veröffentlicht in:	Chemical research in toxicology 2021-06, Vol.34 (6), p.1481-1495
Hauptverfasser:	Kessler, Amanda, Hedberg, Jonas, McCarrick, Sarah, Karlsson, Hanna L, Blomberg, Eva, Odnevall, Inger
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Fluoresceins - chemistry Horseradish Peroxidase - chemistry Horseradish Peroxidase - metabolism Metal Nanoparticles - chemistry Metals, Heavy - chemistry Particle Size Reactive Oxygen Species - analysis Reactive Oxygen Species - metabolism Surface Properties
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