Broad anti-SARS-CoV-2 antibody immunity induced by heterologous ChAdOx1/mRNA-1273 vaccination
Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy. We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-specific serological and memory B cell (MBC) responses in individuals who received either homolo...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2022-03, Vol.375 (6584), p.1041-1047 |
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| creator | Kaku, Chengzi I Champney, Elizabeth R Normark, Johan Garcia, Marina Johnson, Carl E Ahlm, Clas Christ, Wanda Sakharkar, Mrunal Ackerman, Margaret E Klingström, Jonas Forsell, Mattias N E Walker, Laura M |
| description | Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy. We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1:ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) prime-boost vaccination. Heterologous messenger RNA (mRNA) booster immunization induced higher serum neutralizing antibody and MBC responses against SARS-CoV-2 variants of concern (VOCs) compared with that of homologous ChAdOx1 boosting. Specificity mapping of circulating B cells revealed that mRNA-1273 boost immunofocused ChAdOx1-primed responses onto epitopes expressed on prefusion-stabilized S. Monoclonal antibodies isolated from mRNA-1273-boosted participants displayed overall higher binding affinities and increased breadth of reactivity against VOCs relative to those isolated from ChAdOx1-boosted individuals. Overall, the results provide molecular insight into the enhanced quality of the B cell response induced after heterologous mRNA booster vaccination. |
| doi_str_mv | 10.1126/science.abn2688 |
| format | Article |
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We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1:ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) prime-boost vaccination. Heterologous messenger RNA (mRNA) booster immunization induced higher serum neutralizing antibody and MBC responses against SARS-CoV-2 variants of concern (VOCs) compared with that of homologous ChAdOx1 boosting. Specificity mapping of circulating B cells revealed that mRNA-1273 boost immunofocused ChAdOx1-primed responses onto epitopes expressed on prefusion-stabilized S. Monoclonal antibodies isolated from mRNA-1273-boosted participants displayed overall higher binding affinities and increased breadth of reactivity against VOCs relative to those isolated from ChAdOx1-boosted individuals. Overall, the results provide molecular insight into the enhanced quality of the B cell response induced after heterologous mRNA booster vaccination.</description><identifier>ISSN: 0036-8075</identifier><identifier>ISSN: 1095-9203</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.abn2688</identifier><identifier>PMID: 35143256</identifier><language>eng</language><publisher>United States: The American Association for the Advancement of Science</publisher><subject>2019-nCoV Vaccine mRNA-1273 - administration & dosage ; 2019-nCoV Vaccine mRNA-1273 - immunology ; Adult ; Antibodies ; Antibodies, Monoclonal - immunology ; Antibodies, Neutralizing - blood ; Antibodies, Neutralizing - immunology ; Antibodies, Viral - blood ; Antibodies, Viral - immunology ; Antibody Specificity ; ChAdOx1 nCoV-19 - administration & dosage ; ChAdOx1 nCoV-19 - immunology ; Conformation ; Coronaviruses ; COVID-19 Vaccines - immunology ; Epitopes ; Female ; Humans ; Immune response ; Immune system ; Immunity ; Immunization Schedule ; Immunization, Secondary ; Immunogenicity, Vaccine ; Immunology ; Male ; Memory B Cells - immunology ; Middle Aged ; mRNA ; mRNA vaccines ; Pandemics ; Protein Conformation ; Protein Domains ; SARS-CoV-2 - immunology ; Severe acute respiratory syndrome coronavirus 2 ; Spike Glycoprotein, Coronavirus - chemistry ; Spike Glycoprotein, Coronavirus - immunology ; Vaccines ; Viral diseases</subject><ispartof>Science (American Association for the Advancement of Science), 2022-03, Vol.375 (6584), p.1041-1047</ispartof><rights>Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. 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Distributed under a Creative Commons Attribution License 4.0 (CC BY). 2022 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-a4b8738ca01a21442232bdb3f1bd62a2c875e6894689104066ce0dd1a8fd8eb23</citedby><cites>FETCH-LOGICAL-c543t-a4b8738ca01a21442232bdb3f1bd62a2c875e6894689104066ce0dd1a8fd8eb23</cites><orcidid>0000-0002-9854-8351 ; 0000-0003-2018-8592 ; 0000-0002-9130-3933 ; 0000-0003-3886-5248 ; 0000-0001-5831-4369 ; 0000-0001-6904-742X ; 0000-0001-7704-3197 ; 0000-0002-2717-5201 ; 0000-0002-4253-3476 ; 0000-0001-9076-1441</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,550,776,780,881,2871,2872,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35143256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-193058$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:149438966$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaku, Chengzi I</creatorcontrib><creatorcontrib>Champney, Elizabeth R</creatorcontrib><creatorcontrib>Normark, Johan</creatorcontrib><creatorcontrib>Garcia, Marina</creatorcontrib><creatorcontrib>Johnson, Carl E</creatorcontrib><creatorcontrib>Ahlm, Clas</creatorcontrib><creatorcontrib>Christ, Wanda</creatorcontrib><creatorcontrib>Sakharkar, Mrunal</creatorcontrib><creatorcontrib>Ackerman, Margaret E</creatorcontrib><creatorcontrib>Klingström, Jonas</creatorcontrib><creatorcontrib>Forsell, Mattias N E</creatorcontrib><creatorcontrib>Walker, Laura M</creatorcontrib><title>Broad anti-SARS-CoV-2 antibody immunity induced by heterologous ChAdOx1/mRNA-1273 vaccination</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy. We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1:ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) prime-boost vaccination. Heterologous messenger RNA (mRNA) booster immunization induced higher serum neutralizing antibody and MBC responses against SARS-CoV-2 variants of concern (VOCs) compared with that of homologous ChAdOx1 boosting. Specificity mapping of circulating B cells revealed that mRNA-1273 boost immunofocused ChAdOx1-primed responses onto epitopes expressed on prefusion-stabilized S. Monoclonal antibodies isolated from mRNA-1273-boosted participants displayed overall higher binding affinities and increased breadth of reactivity against VOCs relative to those isolated from ChAdOx1-boosted individuals. 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We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1:ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) prime-boost vaccination. Heterologous messenger RNA (mRNA) booster immunization induced higher serum neutralizing antibody and MBC responses against SARS-CoV-2 variants of concern (VOCs) compared with that of homologous ChAdOx1 boosting. Specificity mapping of circulating B cells revealed that mRNA-1273 boost immunofocused ChAdOx1-primed responses onto epitopes expressed on prefusion-stabilized S. Monoclonal antibodies isolated from mRNA-1273-boosted participants displayed overall higher binding affinities and increased breadth of reactivity against VOCs relative to those isolated from ChAdOx1-boosted individuals. 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| subjects | 2019-nCoV Vaccine mRNA-1273 - administration & dosage 2019-nCoV Vaccine mRNA-1273 - immunology Adult Antibodies Antibodies, Monoclonal - immunology Antibodies, Neutralizing - blood Antibodies, Neutralizing - immunology Antibodies, Viral - blood Antibodies, Viral - immunology Antibody Specificity ChAdOx1 nCoV-19 - administration & dosage ChAdOx1 nCoV-19 - immunology Conformation Coronaviruses COVID-19 Vaccines - immunology Epitopes Female Humans Immune response Immune system Immunity Immunization Schedule Immunization, Secondary Immunogenicity, Vaccine Immunology Male Memory B Cells - immunology Middle Aged mRNA mRNA vaccines Pandemics Protein Conformation Protein Domains SARS-CoV-2 - immunology Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - chemistry Spike Glycoprotein, Coronavirus - immunology Vaccines Viral diseases |
| title | Broad anti-SARS-CoV-2 antibody immunity induced by heterologous ChAdOx1/mRNA-1273 vaccination |
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